Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 156936825

GI number: 156936825

Start: 28875

End: 29468

Strand: Direct

Name: hisH [H]

Synonym: Igni_0030

Alternate gene names: 156936825

Gene position: 28875-29468 (Clockwise)

Preceding gene: 156936824

Following gene: 156936827

Centisome position: 2.23

GC content: 52.02

Gene sequence:

>594_bases
GTGCTGGCGGCAATAGTTAACTACGGAATAGGGAACTTATTTAGCGTTAAGAAAGGTTTCGAGAGGGCGGGCTTCAGAGT
TGTAGTATCTAACGACAAAGAGGTCTTGAAGGGAAGCGACGTAATAGTGTTGCCGGGAGTGGGCTCCCACAAGGCCGCCA
TTGCTAACATGACCTCGTTAGGTATAGTTGACGTAATCAAAGAAGTTGACAACTTCAAGTTCGGGATCTGTTTGGGCATG
CAATTGCTTTACGAAGAGAGCGAAGAGGGCGGGCTCAAGGGCCTCGGAATCTTGAAGGGAAAGGTGATTAGGCTTAGAGG
AGTTCCCAAAGTCCCGCATATGGGGTGGAACACCGTAGTGGGGGAGTCGCCTCTGCTCGAGGGCTTGAGCGGCGAGTACT
TCTACTTCGCCCATAGCTACTACAAAGAGTACGAGGGGAGGAAAGAGGAGGAAGCGGTCAGCGATTATGAGGGAGTCAAG
ATAACGGCGCTTGTCTGTTCAGGGAAGGTCTGCGCGACCCAGTTCCACCCCGAGAAGAGCTACGTTAACGGGGTACGACT
GCTGAAGAACTTTTACACGTATGCGAGGCGCTAG

Upstream 100 bases:

>100_bases
GCTGACGTAATCTACCACTTGATGGTGTTGTTAGCCTTGAGAGGGGTGGACTGGGCTGAGGTTATCAAGGAGCTAGAGAG
GAGGTCAAGGGCGAAGAGCG

Downstream 100 bases:

>100_bases
AGCGGTAAGACCTCTTCCACCTCTTCCGGACTCAGCCTGAGGGCGCCCATGCGGGCGTAGCCGCCGATGTCTATGTTGGT
CATCTTTCCGTTCAACACGT

Product: imidazole glycerol phosphate synthase subunit hisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MLAAIVNYGIGNLFSVKKGFERAGFRVVVSNDKEVLKGSDVIVLPGVGSHKAAIANMTSLGIVDVIKEVDNFKFGICLGM
QLLYEESEEGGLKGLGILKGKVIRLRGVPKVPHMGWNTVVGESPLLEGLSGEYFYFAHSYYKEYEGRKEEEAVSDYEGVK
ITALVCSGKVCATQFHPEKSYVNGVRLLKNFYTYARR

Sequences:

>Translated_197_residues
MLAAIVNYGIGNLFSVKKGFERAGFRVVVSNDKEVLKGSDVIVLPGVGSHKAAIANMTSLGIVDVIKEVDNFKFGICLGM
QLLYEESEEGGLKGLGILKGKVIRLRGVPKVPHMGWNTVVGESPLLEGLSGEYFYFAHSYYKEYEGRKEEEAVSDYEGVK
ITALVCSGKVCATQFHPEKSYVNGVRLLKNFYTYARR
>Mature_197_residues
MLAAIVNYGIGNLFSVKKGFERAGFRVVVSNDKEVLKGSDVIVLPGVGSHKAAIANMTSLGIVDVIKEVDNFKFGICLGM
QLLYEESEEGGLKGLGILKGKVIRLRGVPKVPHMGWNTVVGESPLLEGLSGEYFYFAHSYYKEYEGRKEEEAVSDYEGVK
ITALVCSGKVCATQFHPEKSYVNGVRLLKNFYTYARR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=40.1015228426396, Blast_Score=117, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=203, Percent_Identity=30.5418719211823, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21710; Mature: 21710

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAAIVNYGIGNLFSVKKGFERAGFRVVVSNDKEVLKGSDVIVLPGVGSHKAAIANMTSL
CEEEEEECCCCCHHHHHHHHHHCCEEEEEECCHHHHCCCCEEEECCCCCCHHHHHHHHHH
GIVDVIKEVDNFKFGICLGMQLLYEESEEGGLKGLGILKGKVIRLRGVPKVPHMGWNTVV
HHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCC
GESPLLEGLSGEYFYFAHSYYKEYEGRKEEEAVSDYEGVKITALVCSGKVCATQFHPEKS
CCCHHHHCCCCCEEEEHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCEEEEEECCCHH
YVNGVRLLKNFYTYARR
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLAAIVNYGIGNLFSVKKGFERAGFRVVVSNDKEVLKGSDVIVLPGVGSHKAAIANMTSL
CEEEEEECCCCCHHHHHHHHHHCCEEEEEECCHHHHCCCCEEEECCCCCCHHHHHHHHHH
GIVDVIKEVDNFKFGICLGMQLLYEESEEGGLKGLGILKGKVIRLRGVPKVPHMGWNTVV
HHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCC
GESPLLEGLSGEYFYFAHSYYKEYEGRKEEEAVSDYEGVKITALVCSGKVCATQFHPEKS
CCCHHHHCCCCCEEEEHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCEEEEEECCCHH
YVNGVRLLKNFYTYARR
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA