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The map label for this gene is ycgF [H]

Identifier: 156936581

GI number: 156936581

Start: 27774

End: 28994

Strand: Direct

Name: ycgF [H]

Synonym: ESA_pESA3p05462

Alternate gene names: 156936581

Gene position: 27774-28994 (Clockwise)

Preceding gene: 156936579

Following gene: 156936582

Centisome position: 21.17

GC content: 56.84

Gene sequence:

>1221_bases
ATGATGCTCACAACCATAATTTATCGCAGCCATATCTGCGACGACGTTCCGGTCACCGTGCTGGAAGAGATGGTGGATGC
AGCGAATAACAAAAATGGCCAGGCCGACGTGACCGGCATTCTGCTCTTTAACGGCCGCCACTTTTTCCAGCTGCTGGAAG
GCCCGGAAGAAGGGGTTAAAGCTATCTATCGCGCCATTTGCGCGGATACGCGTCATTACAATCTGGTGGAGCTGCTGTGC
GACTACGCGCCGTCGCGTCGCTTCGGTAAATCGGGTATGGAACTCTTTGATTTACGGGATTATGAGCGTGACGAAGTGCT
CCAGCATGTGCTGGATAAAGGCACCTCGAAATACCAGCTGGTGTACGGCGACCGCGCGCTGCAGTTCTTCCAGACGTTTG
TCGAAGCCACTGAAAAAGAAAACTATTTTGAGATCCCGCCGGGTGACAGCTGGGATTTCATCATCGAAGACGACGGCGTT
CAGGCGCCCGCGTCTGTCAGCGCGCGTACCGATTGTACCTTCGCGTTTCAGCCGATTATCGACCCGCTCTCCCGCGAAGT
GGTCTCGCTGGAGGCGCTGCTGCGTACCCCTGACGGCGCGCCGCCGCAGACGTTTTTTGAAGGTCTGGAAGGCGAAGCGC
TGTATGAGGCCGATCTGAAGAGCAAGAAAACGGCGTTTGAAATCGCCGGCGCGCTGAATATTGGCCAGCAGACGCTGTGC
GTAAACCTGCAGCCGATGACCCTGGTGAAAATCCCTGGCGCGGTGGAGTTTCTGGTACAGGAGATCCACGCCAACGGGCT
GGTGCCGGATCAGGTGATTGTTGAGTTTACCGAAAGCGAAGTGATTTCCCGTCTCGATGAGTTTACCGGTGCGATTCGCC
AGCTAAAAGCGGCGGGGCTGCGCGTGGCGATTGACCACTTTGGTGCCGGTTTCGCCGGGCTGTTGCTGCTGGCGCAGTTC
CAGCCAGACCGCATCAAAATCAACCGCGAGCTTATTCGCGATGTGCATAAGAGCGGCCCGCGACAGGCCATTGTGCAGGC
GCTGATCAAATGCTGCGCGTCGCTGGAAATCGCGATTTCTGCCGTGGGCGTGGAGAAAGCCGAAGAGTGGATGTGGCTCG
AATCCGCAGGCATTACGCTCTTCCAGGGGCATCTTTTCGCAAAACCAGCGCTCGGTGGCATTCCTGCCGTCGCCTGGCCT
GAAAAAAAGTTCGGCTTGTAA

Upstream 100 bases:

>100_bases
CCTTACCTGACTCATTATTTGTCTCTTTATAGACATCACTGCAAGACGCCCGGCAGGTCATCTGTTAAGGTGGCTGCTTA
TTCACCTATTCACAGGATGC

Downstream 100 bases:

>100_bases
CGGTTTGACCTTCTCTCTGGCGCATGTCAAAGCCGCATGCGCCATCTCTCTGTGATTTAATGCATTTTATGTACACTTTT
TTGTTGCCCGCAGCACGTTG

Product: hypothetical protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MMLTTIIYRSHICDDVPVTVLEEMVDAANNKNGQADVTGILLFNGRHFFQLLEGPEEGVKAIYRAICADTRHYNLVELLC
DYAPSRRFGKSGMELFDLRDYERDEVLQHVLDKGTSKYQLVYGDRALQFFQTFVEATEKENYFEIPPGDSWDFIIEDDGV
QAPASVSARTDCTFAFQPIIDPLSREVVSLEALLRTPDGAPPQTFFEGLEGEALYEADLKSKKTAFEIAGALNIGQQTLC
VNLQPMTLVKIPGAVEFLVQEIHANGLVPDQVIVEFTESEVISRLDEFTGAIRQLKAAGLRVAIDHFGAGFAGLLLLAQF
QPDRIKINRELIRDVHKSGPRQAIVQALIKCCASLEIAISAVGVEKAEEWMWLESAGITLFQGHLFAKPALGGIPAVAWP
EKKFGL

Sequences:

>Translated_406_residues
MMLTTIIYRSHICDDVPVTVLEEMVDAANNKNGQADVTGILLFNGRHFFQLLEGPEEGVKAIYRAICADTRHYNLVELLC
DYAPSRRFGKSGMELFDLRDYERDEVLQHVLDKGTSKYQLVYGDRALQFFQTFVEATEKENYFEIPPGDSWDFIIEDDGV
QAPASVSARTDCTFAFQPIIDPLSREVVSLEALLRTPDGAPPQTFFEGLEGEALYEADLKSKKTAFEIAGALNIGQQTLC
VNLQPMTLVKIPGAVEFLVQEIHANGLVPDQVIVEFTESEVISRLDEFTGAIRQLKAAGLRVAIDHFGAGFAGLLLLAQF
QPDRIKINRELIRDVHKSGPRQAIVQALIKCCASLEIAISAVGVEKAEEWMWLESAGITLFQGHLFAKPALGGIPAVAWP
EKKFGL
>Mature_406_residues
MMLTTIIYRSHICDDVPVTVLEEMVDAANNKNGQADVTGILLFNGRHFFQLLEGPEEGVKAIYRAICADTRHYNLVELLC
DYAPSRRFGKSGMELFDLRDYERDEVLQHVLDKGTSKYQLVYGDRALQFFQTFVEATEKENYFEIPPGDSWDFIIEDDGV
QAPASVSARTDCTFAFQPIIDPLSREVVSLEALLRTPDGAPPQTFFEGLEGEALYEADLKSKKTAFEIAGALNIGQQTLC
VNLQPMTLVKIPGAVEFLVQEIHANGLVPDQVIVEFTESEVISRLDEFTGAIRQLKAAGLRVAIDHFGAGFAGLLLLAQF
QPDRIKINRELIRDVHKSGPRQAIVQALIKCCASLEIAISAVGVEKAEEWMWLESAGITLFQGHLFAKPALGGIPAVAWP
EKKFGL

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=403, Percent_Identity=66.0049627791563, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1787541, Length=240, Percent_Identity=31.6666666666667, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788502, Length=227, Percent_Identity=25.9911894273128, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082096, Length=151, Percent_Identity=31.1258278145695, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI226510982, Length=224, Percent_Identity=29.9107142857143, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787055, Length=238, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790496, Length=224, Percent_Identity=27.2321428571429, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081743, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081921, Length=232, Percent_Identity=26.7241379310345, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789650, Length=117, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081980, Length=224, Percent_Identity=23.6607142857143, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 45023; Mature: 45023

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50925 BLUF ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLTTIIYRSHICDDVPVTVLEEMVDAANNKNGQADVTGILLFNGRHFFQLLEGPEEGVK
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHCCHHHHHH
AIYRAICADTRHYNLVELLCDYAPSRRFGKSGMELFDLRDYERDEVLQHVLDKGTSKYQL
HHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCEEHHCCCCCHHHHHHHHHHCCCCEEEE
VYGDRALQFFQTFVEATEKENYFEIPPGDSWDFIIEDDGVQAPASVSARTDCTFAFQPII
EECHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCCEEEHHHHH
DPLSREVVSLEALLRTPDGAPPQTFFEGLEGEALYEADLKSKKTAFEIAGALNIGQQTLC
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEHHHHHCCHHHHHHHHHHCCCCEEEE
VNLQPMTLVKIPGAVEFLVQEIHANGLVPDQVIVEFTESEVISRLDEFTGAIRQLKAAGL
EECCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVAIDHFGAGFAGLLLLAQFQPDRIKINRELIRDVHKSGPRQAIVQALIKCCASLEIAIS
EEEHHHHCCHHHHHHHHHHCCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AVGVEKAEEWMWLESAGITLFQGHLFAKPALGGIPAVAWPEKKFGL
HHCHHHHHHHEEHCCCCCEEEECCEEECCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MMLTTIIYRSHICDDVPVTVLEEMVDAANNKNGQADVTGILLFNGRHFFQLLEGPEEGVK
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHCCHHHHHH
AIYRAICADTRHYNLVELLCDYAPSRRFGKSGMELFDLRDYERDEVLQHVLDKGTSKYQL
HHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCEEHHCCCCCHHHHHHHHHHCCCCEEEE
VYGDRALQFFQTFVEATEKENYFEIPPGDSWDFIIEDDGVQAPASVSARTDCTFAFQPII
EECHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCCEEEHHHHH
DPLSREVVSLEALLRTPDGAPPQTFFEGLEGEALYEADLKSKKTAFEIAGALNIGQQTLC
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEHHHHHCCHHHHHHHHHHCCCCEEEE
VNLQPMTLVKIPGAVEFLVQEIHANGLVPDQVIVEFTESEVISRLDEFTGAIRQLKAAGL
EECCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVAIDHFGAGFAGLLLLAQFQPDRIKINRELIRDVHKSGPRQAIVQALIKCCASLEIAIS
EEEHHHHCCHHHHHHHHHHCCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AVGVEKAEEWMWLESAGITLFQGHLFAKPALGGIPAVAWPEKKFGL
HHCHHHHHHHEEHCCCCCEEEECCEEECCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]