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Identifier: 15679862

GI number: 15679862

Start: 1709290

End: 1710084

Strand: Direct

Name: Not Available

Synonym: MTH1874

Alternate gene names: 15679862

Gene position: 1709290-1710084 (Clockwise)

Preceding gene: 15679860

Following gene: 15679864

Centisome position: 97.6

GC content: 51.57

Gene sequence:

>795_bases
ATGCTATACATAATTGGACTGGGACTCTACGACGAGAATGACATATCAATCAAGGGCCTGAGGGCCATAGAGGACTGTGA
CGAGGTTTACGCTGAATTCTACACTGCAATGCTCCAGGGGTCCAGTTTATCGGCAATCGAGAGGAGAATAGGTAGGAGTG
TGAAGGTCCTGGGGAGGGGAGAAATCGAGGAGGAAAGAATCCCCATTGAAAGGGCCCTGGAGCTTGATGTGGCCCTCCTT
GTTGCAGGGGATCCCCTTGTTGCAACAACACACACGGAGCTTCTCATAGACGCCCACAGGAGGGGCATAGAGACAAGGGT
TATCCATTCGTCATCCATAATATCAGCCGCTCCTGGTCTCGCAGGCCTTCAGGCCTACAAGTTTGGAAGGATAATCACAA
TACCATTCACCTCTGATAACTACTTTCCCACATCACCCTACACCAACATAGGGGAGAACCTTAAGGCAGGTTCCCACTCC
CTTGTGCTCCTGGATATCGAGGCCCACAGGAATAGATACATGACGGCAACTGAGGGGCTCAGGTATCTCCTGAAGGCATC
AGAGAAACTTGATGATGGTACAATTAGTGGCGAAACCCTTGCAGTTGTCATAGCAAGGGCTGGCTCTGAGAAGCCCCTTG
TGAGGGCTGATAAAATAGCTACACTCGTTGAGGAGGACTTCGGTGATCCACTCCACTGTCTTGTGGTCCCGGCGGGCCTT
CATTTCATTGAGGCGGACTATCTTGTTGAGATTGCAGGGGCACCCCGGGCAGTGGTGGAGAGGATGATCCTATAG

Upstream 100 bases:

>100_bases
TCATGATTGAAACTTCCCCTATCTTGAGTCACTTATTATATTGGTCACGTGCATATATACCGTTATTAATGGTTTCTGAT
ACTGATTAGGGAGGGTTAAG

Downstream 100 bases:

>100_bases
CCTATCTCAGGAGATACTCACATTTTTCAACATTCTGGAGGGGGATGATTATCTCCCTTTTCCCGAATTCCCTCTCAGAG
AAGCTCACCAGTATGACGCA

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MLYIIGLGLYDENDISIKGLRAIEDCDEVYAEFYTAMLQGSSLSAIERRIGRSVKVLGRGEIEEERIPIERALELDVALL
VAGDPLVATTHTELLIDAHRRGIETRVIHSSSIISAAPGLAGLQAYKFGRIITIPFTSDNYFPTSPYTNIGENLKAGSHS
LVLLDIEAHRNRYMTATEGLRYLLKASEKLDDGTISGETLAVVIARAGSEKPLVRADKIATLVEEDFGDPLHCLVVPAGL
HFIEADYLVEIAGAPRAVVERMIL

Sequences:

>Translated_264_residues
MLYIIGLGLYDENDISIKGLRAIEDCDEVYAEFYTAMLQGSSLSAIERRIGRSVKVLGRGEIEEERIPIERALELDVALL
VAGDPLVATTHTELLIDAHRRGIETRVIHSSSIISAAPGLAGLQAYKFGRIITIPFTSDNYFPTSPYTNIGENLKAGSHS
LVLLDIEAHRNRYMTATEGLRYLLKASEKLDDGTISGETLAVVIARAGSEKPLVRADKIATLVEEDFGDPLHCLVVPAGL
HFIEADYLVEIAGAPRAVVERMIL
>Mature_264_residues
MLYIIGLGLYDENDISIKGLRAIEDCDEVYAEFYTAMLQGSSLSAIERRIGRSVKVLGRGEIEEERIPIERALELDVALL
VAGDPLVATTHTELLIDAHRRGIETRVIHSSSIISAAPGLAGLQAYKFGRIITIPFTSDNYFPTSPYTNIGENLKAGSHS
LVLLDIEAHRNRYMTATEGLRYLLKASEKLDDGTISGETLAVVIARAGSEKPLVRADKIATLVEEDFGDPLHCLVVPAGL
HFIEADYLVEIAGAPRAVVERMIL

Specific function: Required for the methylation step in diphthamide biosynthesis

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family

Homologues:

Organism=Homo sapiens, GI117190326, Length=269, Percent_Identity=36.4312267657993, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI117190262, Length=269, Percent_Identity=36.4312267657993, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI117190324, Length=268, Percent_Identity=36.5671641791045, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531369, Length=269, Percent_Identity=35.3159851301115, Blast_Score=151, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6323201, Length=252, Percent_Identity=38.0952380952381, Blast_Score=166, Evalue=3e-42,
Organism=Drosophila melanogaster, GI28572120, Length=273, Percent_Identity=36.6300366300366, Blast_Score=160, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPHB_METTH (O27902)

Other databases:

- EMBL:   AE000666
- PIR:   G69117
- RefSeq:   NP_276980.1
- ProteinModelPortal:   O27902
- SMR:   O27902
- STRING:   O27902
- GeneID:   1470959
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1874
- NMPDR:   fig|187420.1.peg.1835
- eggNOG:   arNOG04257
- HOGENOM:   HBG460511
- OMA:   YAEFYTA
- PhylomeDB:   O27902
- ProtClustDB:   PRK04160
- BioCyc:   MTHE187420:MTH1874-MONOMER
- BRENDA:   2.1.1.98
- HAMAP:   MF_01084
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- PANTHER:   PTHR10882
- PIRSF:   PIRSF036432
- TIGRFAMs:   TIGR00522

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.98

Molecular weight: Translated: 28859; Mature: 28859

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYIIGLGLYDENDISIKGLRAIEDCDEVYAEFYTAMLQGSSLSAIERRIGRSVKVLGRG
CEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEECC
EIEEERIPIERALELDVALLVAGDPLVATTHTELLIDAHRRGIETRVIHSSSIISAAPGL
CCCHHCCCHHHHHEEEEEEEEECCCEEEECHHHHHHHHHHCCCEEEEEECCCHHHCCCCC
AGLQAYKFGRIITIPFTSDNYFPTSPYTNIGENLKAGSHSLVLLDIEAHRNRYMTATEGL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEHHHHH
RYLLKASEKLDDGTISGETLAVVIARAGSEKPLVRADKIATLVEEDFGDPLHCLVVPAGL
HHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
HFIEADYLVEIAGAPRAVVERMIL
EEEECCEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MLYIIGLGLYDENDISIKGLRAIEDCDEVYAEFYTAMLQGSSLSAIERRIGRSVKVLGRG
CEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEECC
EIEEERIPIERALELDVALLVAGDPLVATTHTELLIDAHRRGIETRVIHSSSIISAAPGL
CCCHHCCCHHHHHEEEEEEEEECCCEEEECHHHHHHHHHHCCCEEEEEECCCHHHCCCCC
AGLQAYKFGRIITIPFTSDNYFPTSPYTNIGENLKAGSHSLVLLDIEAHRNRYMTATEGL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEHHHHH
RYLLKASEKLDDGTISGETLAVVIARAGSEKPLVRADKIATLVEEDFGDPLHCLVVPAGL
HHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
HFIEADYLVEIAGAPRAVVERMIL
EEEECCEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463