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Identifier: 15679626

GI number: 15679626

Start: 1495715

End: 1497001

Strand: Direct

Name: Not Available

Synonym: MTH1631

Alternate gene names: 15679626

Gene position: 1495715-1497001 (Clockwise)

Preceding gene: 15679625

Following gene: 15679627

Centisome position: 85.4

GC content: 55.79

Gene sequence:

>1287_bases
GTGGTTAAAATGAACATGACAGAGAAGATCCTCGCAGAGGCAGCAGGACTCAGGGAGGTCACACCCGGCGAGATAATAGA
GGCAAGGGTTGACCTCGCCATGACCCATGACGGCACCTCACCCCCCACCATCAGGACCTTCAGGGATATAGCCTCAAGGG
GAGGCCCCGCTAGGGTCTGGGACCCCGAGAGGATAGTCATGGTATTTGATCACAACGTACCAGCCAACACAATAGGTGCC
GCGGAGTTCCAGAGGGTCACAAGGGAATTCGCCAGGGAACAGGGTATAGTGAACATATTCCAGAACGCAGCAGGCATATG
CCACCAGGTTCTGCCTGAGAGGGGATTTGTAAGACCTGGCATGGTCATTGTTGGGGCGGACTCCCACACCTGCACCTACG
GAGCCTTCGGAGCCTTCGCAACAGGCATGGGGGCGACTGACATGGCCATGGTCTTTGCAACAGGCAAAACCTGGTTCATG
GTACCTGAGGCAATGAGAATCGAGGTTACAGGTGAACCTGAGGGCCACGTGTATGCAAAGGACGTCATACTCCACATCAT
AGGCGAGATCGGTGTTGATGGTGCAACCTACCGTTCCGTGGAATTCACAGGAGACACCATAGAGAGCATGGATGTCTCCG
GGAGGATGACCATCTGCAACATGGCCGTGGAGATGGGTGCAAAGAACGGTATAATGGAGCCCAACCGGCAGACCCTTGAC
TATGTGAGGGCAAGGACAGGCAGGGAATTCAGGGTTTACAGTTCAGATGAGGACAGCCAGTACCTGGAGGATCACCACTT
CGATGTCTCAGACCTTGAACCCCAGGTGGCCTGTCCAGATGATGTTGACAACGTCTACCCTGTCCACAGGGTTGAGGGAA
CCCACATAGACGAGGCCTTCCTGGGTTCATGCACCAACGGCCGGTACGAGGACCTGAAAATCGCCGCAGAGGTTATAGGC
GACAGGAGGGTCCATGAGGATGTGAGGTTCATAGTCTCGCCGGCATCAAGGGAGATCTACCTTAAGGCACTGGAGGATGG
TATAATAGAGACCTTCATCAGGGCAGGGGCGATAGTCTGCAACCCTGGCTGCGGCCCGTGCCTGGGGGCCCATATGGGAG
TCCTTGCCCCGGGGGAGGTCAGCATAGCAACAACCAACAGAAACTTCAGGGGAAGGATGGGTGACCCTGCATCCAGCGTC
TACCTGGCCAACCCGGCAGTCGTCGCAGAATCCGCCATTGAAGGGGTAATAAGCGCCCCTCAACAGGAGGCAGGAAATGG
ATGTTAA

Upstream 100 bases:

>100_bases
AGTGGAGGAGGTAAAGAAAAACAGGGAGAAGGGTAAGTACATCAACGATGAACTCTTCTACAGGATTGTGAAGTCCGTAA
GGGGACCTGTAGACTTCTAG

Downstream 100 bases:

>100_bases
TGTCATGGAGGAGATTGAACGTATTGAGAGGCTCATAGGGAAACTCTCAAACACCCAGAAGATACTGCTCTCAACAGACG
GCTCGGTAACAAGGATACTT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVKMNMTEKILAEAAGLREVTPGEIIEARVDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPERIVMVFDHNVPANTIGA
AEFQRVTREFAREQGIVNIFQNAAGICHQVLPERGFVRPGMVIVGADSHTCTYGAFGAFATGMGATDMAMVFATGKTWFM
VPEAMRIEVTGEPEGHVYAKDVILHIIGEIGVDGATYRSVEFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLD
YVRARTGREFRVYSSDEDSQYLEDHHFDVSDLEPQVACPDDVDNVYPVHRVEGTHIDEAFLGSCTNGRYEDLKIAAEVIG
DRRVHEDVRFIVSPASREIYLKALEDGIIETFIRAGAIVCNPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGDPASSV
YLANPAVVAESAIEGVISAPQQEAGNGC

Sequences:

>Translated_428_residues
MVKMNMTEKILAEAAGLREVTPGEIIEARVDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPERIVMVFDHNVPANTIGA
AEFQRVTREFAREQGIVNIFQNAAGICHQVLPERGFVRPGMVIVGADSHTCTYGAFGAFATGMGATDMAMVFATGKTWFM
VPEAMRIEVTGEPEGHVYAKDVILHIIGEIGVDGATYRSVEFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLD
YVRARTGREFRVYSSDEDSQYLEDHHFDVSDLEPQVACPDDVDNVYPVHRVEGTHIDEAFLGSCTNGRYEDLKIAAEVIG
DRRVHEDVRFIVSPASREIYLKALEDGIIETFIRAGAIVCNPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGDPASSV
YLANPAVVAESAIEGVISAPQQEAGNGC
>Mature_428_residues
MVKMNMTEKILAEAAGLREVTPGEIIEARVDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPERIVMVFDHNVPANTIGA
AEFQRVTREFAREQGIVNIFQNAAGICHQVLPERGFVRPGMVIVGADSHTCTYGAFGAFATGMGATDMAMVFATGKTWFM
VPEAMRIEVTGEPEGHVYAKDVILHIIGEIGVDGATYRSVEFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLD
YVRARTGREFRVYSSDEDSQYLEDHHFDVSDLEPQVACPDDVDNVYPVHRVEGTHIDEAFLGSCTNGRYEDLKIAAEVIG
DRRVHEDVRFIVSPASREIYLKALEDGIIETFIRAGAIVCNPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGDPASSV
YLANPAVVAESAIEGVISAPQQEAGNGC

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=351, Percent_Identity=31.3390313390313, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1786259, Length=439, Percent_Identity=33.7129840546697, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87081781, Length=352, Percent_Identity=25.2840909090909, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI2367097, Length=434, Percent_Identity=22.1198156682028, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787531, Length=502, Percent_Identity=22.3107569721116, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32564738, Length=380, Percent_Identity=29.2105263157895, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI25149337, Length=380, Percent_Identity=29.2105263157895, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=278, Percent_Identity=30.2158273381295, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=466, Percent_Identity=22.9613733905579, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=450, Percent_Identity=32.6666666666667, Blast_Score=226, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6321429, Length=434, Percent_Identity=29.7235023041475, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6323335, Length=343, Percent_Identity=32.6530612244898, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6322261, Length=354, Percent_Identity=29.9435028248588, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI161076999, Length=344, Percent_Identity=30.5232558139535, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI281365315, Length=344, Percent_Identity=30.5232558139535, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI17864292, Length=344, Percent_Identity=30.5232558139535, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI28571643, Length=450, Percent_Identity=28.2222222222222, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI17137564, Length=521, Percent_Identity=22.8406909788868, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC2_METTH (O27668)

Other databases:

- EMBL:   AE000666
- PIR:   A69085
- RefSeq:   NP_276743.1
- ProteinModelPortal:   O27668
- STRING:   O27668
- GeneID:   1470716
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1631
- NMPDR:   fig|187420.1.peg.1598
- eggNOG:   arNOG04337
- HOGENOM:   HBG330745
- OMA:   PANTIGS
- PhylomeDB:   O27668
- ProtClustDB:   PRK00402
- BioCyc:   MTHE187420:MTH1631-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 46448; Mature: 46448

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKMNMTEKILAEAAGLREVTPGEIIEARVDLAMTHDGTSPPTIRTFRDIASRGGPARVW
CEECCHHHHHHHHHCCCCCCCCCHHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCCEEE
DPERIVMVFDHNVPANTIGAAEFQRVTREFAREQGIVNIFQNAAGICHQVLPERGFVRPG
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
MVIVGADSHTCTYGAFGAFATGMGATDMAMVFATGKTWFMVPEAMRIEVTGEPEGHVYAK
EEEEECCCCCEECCCHHHHHCCCCCCCEEEEEECCCEEEEECCEEEEEEECCCCCCEEHH
DVILHIIGEIGVDGATYRSVEFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLD
HHHHHHHHHHCCCCCEEEEEEECCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHH
YVRARTGREFRVYSSDEDSQYLEDHHFDVSDLEPQVACPDDVDNVYPVHRVEGTHIDEAF
HHHHCCCCEEEEECCCCCHHHHHHCCCCHHHCCCCCCCCCCCCCCCCEEECCCCCCCHHH
LGSCTNGRYEDLKIAAEVIGDRRVHEDVRFIVSPASREIYLKALEDGIIETFIRAGAIVC
HCCCCCCCHHHHHHHHHHHCCHHHHHHHHEEECCCCCEEEHHHHHHHHHHHHHHCCCEEE
NPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGDPASSVYLANPAVVAESAIEGVISAP
CCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCC
QQEAGNGC
HHHCCCCC
>Mature Secondary Structure
MVKMNMTEKILAEAAGLREVTPGEIIEARVDLAMTHDGTSPPTIRTFRDIASRGGPARVW
CEECCHHHHHHHHHCCCCCCCCCHHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCCEEE
DPERIVMVFDHNVPANTIGAAEFQRVTREFAREQGIVNIFQNAAGICHQVLPERGFVRPG
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
MVIVGADSHTCTYGAFGAFATGMGATDMAMVFATGKTWFMVPEAMRIEVTGEPEGHVYAK
EEEEECCCCCEECCCHHHHHCCCCCCCEEEEEECCCEEEEECCEEEEEEECCCCCCEEHH
DVILHIIGEIGVDGATYRSVEFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLD
HHHHHHHHHHCCCCCEEEEEEECCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHH
YVRARTGREFRVYSSDEDSQYLEDHHFDVSDLEPQVACPDDVDNVYPVHRVEGTHIDEAF
HHHHCCCCEEEEECCCCCHHHHHHCCCCHHHCCCCCCCCCCCCCCCCEEECCCCCCCHHH
LGSCTNGRYEDLKIAAEVIGDRRVHEDVRFIVSPASREIYLKALEDGIIETFIRAGAIVC
HCCCCCCCHHHHHHHHHHHCCHHHHHHHHEEECCCCCEEEHHHHHHHHHHHHHHCCCEEE
NPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGDPASSVYLANPAVVAESAIEGVISAP
CCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCC
QQEAGNGC
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463