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The map label for this gene is aksA

Identifier: 15679625

GI number: 15679625

Start: 1494539

End: 1495714

Strand: Direct

Name: aksA

Synonym: MTH1630

Alternate gene names: 15679625

Gene position: 1494539-1495714 (Clockwise)

Preceding gene: 15679624

Following gene: 15679626

Centisome position: 85.34

GC content: 49.57

Gene sequence:

>1176_bases
TTGAGATACTTTGTTAGCCCCTTCAATAAAGAAGCTGAATTAAAATTTCCTGACAGGATCACGATTTACGATACAACACT
GCGTGACGGGGAGCAGACCCCCGGTGTATGCCTTGGAACAGAAGAAAAACTTGAAATAGCCAGAAAACTTGATGAGCTCG
GCATACATCAGATAGAGAGCGGATTTCCGGTTGTATCAGAACAGGAAAGAGTTTCAGTGAAGTCCATTGCAAATGAGGGC
CTCAATGCAGAGATACTGGCACTCTGCAGGACAAAGAAGGATGACATCGACGCAGCAATAGACTGTGATGTGGATGGAGT
CATAACCTTCATGGCAACATCGGACCTCCACCTGAAACACAAACTCAAACTCACAAGGGAGGAGGCCCTCAACGTCTGTA
TGAACTCCATAGAGTATGCCAAGGATCACGGGCTTTTCCTGGCATTTTCAGCAGAGGATGCAACAAGGACAGACCTCGAC
TTCCTCAAGCAGATCTACCGGAAGGCCGAGAACTATGGTGCAGACAGGGTCCACATTGCAGATACCGTGGGCGCCATAAG
CCCCCAGGGTATGGACTACCTTGTCAGGGAACTGCGCAGGGACATCAAGGTTGACATAGCACTCCACTGCCACAACGACT
TTGGAATGGCCCTATCGAACTCAATAGCAGGGCTACTTGCAGGTGGGACTGCAGTCTCAACCACAGTTAACGGTATAGGT
GAAAGGGCGGGCAACACATCCCTTGAGGAGCTGATCATGGCCCTGAGGATAATCTATGAGGTTGACCTCGGATTCAACAT
AGGCGTCCTCTATGAACTTTCAAGGCTTGTGGAGAAACACACAAGAATGAAGGTACCTGAGAACAAGCCGATAGTCGGCA
GGAACGTATTCAGACACGAATCAGGTATACACGTCGACGCGGTCATAGAGGAGCCCCTAACCTATGAGCCATTCCTTCCA
GAGATGATAGGCCACCAGCGCAAGATAGTGCTGGGGAAACACTCCGGGTGCCGTGCGGTCAAGGCCAAACTTGAGGAGTA
CGGCATCGATGTTACGAGGGATGAACTCTGCAGGATAGTGGAGGAGGTAAAGAAAAACAGGGAGAAGGGTAAGTACATCA
ACGATGAACTCTTCTACAGGATTGTGAAGTCCGTAAGGGGACCTGTAGACTTCTAG

Upstream 100 bases:

>100_bases
ACCGGAATATGGATGCACTCTCAAACCTTTTATACTAATATATCTAATTTTAAATAAATGCCAAGTATTCATTTTAATAA
AGCATTAAGGTGGTAATCCC

Downstream 100 bases:

>100_bases
GTGGTTAAAATGAACATGACAGAGAAGATCCTCGCAGAGGCAGCAGGACTCAGGGAGGTCACACCCGGCGAGATAATAGA
GGCAAGGGTTGACCTCGCCA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRYFVSPFNKEAELKFPDRITIYDTTLRDGEQTPGVCLGTEEKLEIARKLDELGIHQIESGFPVVSEQERVSVKSIANEG
LNAEILALCRTKKDDIDAAIDCDVDGVITFMATSDLHLKHKLKLTREEALNVCMNSIEYAKDHGLFLAFSAEDATRTDLD
FLKQIYRKAENYGADRVHIADTVGAISPQGMDYLVRELRRDIKVDIALHCHNDFGMALSNSIAGLLAGGTAVSTTVNGIG
ERAGNTSLEELIMALRIIYEVDLGFNIGVLYELSRLVEKHTRMKVPENKPIVGRNVFRHESGIHVDAVIEEPLTYEPFLP
EMIGHQRKIVLGKHSGCRAVKAKLEEYGIDVTRDELCRIVEEVKKNREKGKYINDELFYRIVKSVRGPVDF

Sequences:

>Translated_391_residues
MRYFVSPFNKEAELKFPDRITIYDTTLRDGEQTPGVCLGTEEKLEIARKLDELGIHQIESGFPVVSEQERVSVKSIANEG
LNAEILALCRTKKDDIDAAIDCDVDGVITFMATSDLHLKHKLKLTREEALNVCMNSIEYAKDHGLFLAFSAEDATRTDLD
FLKQIYRKAENYGADRVHIADTVGAISPQGMDYLVRELRRDIKVDIALHCHNDFGMALSNSIAGLLAGGTAVSTTVNGIG
ERAGNTSLEELIMALRIIYEVDLGFNIGVLYELSRLVEKHTRMKVPENKPIVGRNVFRHESGIHVDAVIEEPLTYEPFLP
EMIGHQRKIVLGKHSGCRAVKAKLEEYGIDVTRDELCRIVEEVKKNREKGKYINDELFYRIVKSVRGPVDF
>Mature_391_residues
MRYFVSPFNKEAELKFPDRITIYDTTLRDGEQTPGVCLGTEEKLEIARKLDELGIHQIESGFPVVSEQERVSVKSIANEG
LNAEILALCRTKKDDIDAAIDCDVDGVITFMATSDLHLKHKLKLTREEALNVCMNSIEYAKDHGLFLAFSAEDATRTDLD
FLKQIYRKAENYGADRVHIADTVGAISPQGMDYLVRELRRDIKVDIALHCHNDFGMALSNSIAGLLAGGTAVSTTVNGIG
ERAGNTSLEELIMALRIIYEVDLGFNIGVLYELSRLVEKHTRMKVPENKPIVGRNVFRHESGIHVDAVIEEPLTYEPFLP
EMIGHQRKIVLGKHSGCRAVKAKLEEYGIDVTRDELCRIVEEVKKNREKGKYINDELFYRIVKSVRGPVDF

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=287, Percent_Identity=22.6480836236934, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786261, Length=345, Percent_Identity=37.9710144927536, Blast_Score=216, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6320019, Length=352, Percent_Identity=34.6590909090909, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6320071, Length=348, Percent_Identity=34.7701149425287, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324225, Length=400, Percent_Identity=25, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324682, Length=433, Percent_Identity=22.8637413394919, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582381, Length=288, Percent_Identity=23.6111111111111, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU11_METTH (O27667)

Other databases:

- EMBL:   AE000666
- PIR:   H69084
- RefSeq:   NP_276742.1
- ProteinModelPortal:   O27667
- SMR:   O27667
- STRING:   O27667
- GeneID:   1470715
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1630
- NMPDR:   fig|187420.1.peg.1597
- eggNOG:   arNOG04336
- HOGENOM:   HBG701974
- OMA:   QIEAGFP
- PhylomeDB:   O27667
- ProtClustDB:   PRK11858
- BioCyc:   MTHE187420:MTH1630-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.13

Molecular weight: Translated: 43967; Mature: 43967

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYFVSPFNKEAELKFPDRITIYDTTLRDGEQTPGVCLGTEEKLEIARKLDELGIHQIES
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHCHHHHHC
GFPVVSEQERVSVKSIANEGLNAEILALCRTKKDDIDAAIDCDVDGVITFMATSDLHLKH
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCHH
KLKLTREEALNVCMNSIEYAKDHGLFLAFSAEDATRTDLDFLKQIYRKAENYGADRVHIA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEH
DTVGAISPQGMDYLVRELRRDIKVDIALHCHNDFGMALSNSIAGLLAGGTAVSTTVNGIG
HHHCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
ERAGNTSLEELIMALRIIYEVDLGFNIGVLYELSRLVEKHTRMKVPENKPIVGRNVFRHE
HCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCC
SGIHVDAVIEEPLTYEPFLPEMIGHQRKIVLGKHSGCRAVKAKLEEYGIDVTRDELCRIV
CCCEEEEEECCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHH
EEVKKNREKGKYINDELFYRIVKSVRGPVDF
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRYFVSPFNKEAELKFPDRITIYDTTLRDGEQTPGVCLGTEEKLEIARKLDELGIHQIES
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHCHHHHHC
GFPVVSEQERVSVKSIANEGLNAEILALCRTKKDDIDAAIDCDVDGVITFMATSDLHLKH
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCHH
KLKLTREEALNVCMNSIEYAKDHGLFLAFSAEDATRTDLDFLKQIYRKAENYGADRVHIA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEH
DTVGAISPQGMDYLVRELRRDIKVDIALHCHNDFGMALSNSIAGLLAGGTAVSTTVNGIG
HHHCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
ERAGNTSLEELIMALRIIYEVDLGFNIGVLYELSRLVEKHTRMKVPENKPIVGRNVFRHE
HCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCC
SGIHVDAVIEEPLTYEPFLPEMIGHQRKIVLGKHSGCRAVKAKLEEYGIDVTRDELCRIV
CCCEEEEEECCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHH
EEVKKNREKGKYINDELFYRIVKSVRGPVDF
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463