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Identifier: 15679559

GI number: 15679559

Start: 1428437

End: 1429846

Strand: Direct

Name: Not Available

Synonym: MTH1563

Alternate gene names: 15679559

Gene position: 1428437-1429846 (Clockwise)

Preceding gene: 15679558

Following gene: 15679560

Centisome position: 81.56

GC content: 44.96

Gene sequence:

>1410_bases
ATGCCATTTAAACTGTTTGATGTTGACGCTGAAATACCTGAGAGAAAAAAACATGTCTATATAAAGAAGAAGGAAGATCC
CGAAGAGGATCTGCCCTTATGTAACACAAAGACAATCCCTGGTTGCATGACTGAGAGGGGCTGCGCCTTTGCGGGTGCGA
AGGGTGTCATAACCGGTGCAATAAAGGATGCTCTGCATGTTATCCATTCCCCTGTTGGATGCACAGCATATGGCTACGGT
ACAAAGAGGTATCCCACATCACAGGAAATGCCTGATGGCAGCCTTTTTCCCATTGAAAACTTCAACCTGAAGTATATAAC
GGGCACAAATCTCACAGAGACAGATGTTGTGTTTGGGGGTATGGATAAACTCAAAAGATGCATCATCGAGGCTGCTAAAG
AATTCCCAGAGGCAAATGCTGTTTACACATATGCAACATGCACAACCGGTCTCATAGGAGATGATATTGAAGCCGTTTCA
AGGGAGGTTTCAGAAGAAATAGGGAAGGACGTGGTTGCAATAAATGCCCCCGGGTTTGCGGGTCCAACACAGTCAAAGGG
GCATCAGGTTGCAAACTACACACTGTTTGAGAACCTTGTGGGAACCGCAGAGCCTCCGGTAGTAACAGACTATGATGTAA
ACCTCATAGGAGAGTACAACATCGATGGTGACCTCTGGGTTCTCAAGAGATACTTCGAGGAGATGGGAATAAATGTCCTC
AGCACATTCACTGGAGACTGCTGCCATGATGAGATAAAATGGATGCACAGAGCTAAGCTTAGCCTGGTCCGGTGTCAGCG
TTCAGCCACATATATTGCAAGGCTTCTGGAGGAACGTTACAATGTTCCATACATGAAGGTTGATTTCTTTGGAATAGAAT
ACTGCAAGAGGAATCTCATGGCTACAGGGGAATACTTTGGGATTCCTGAAAGGGCAGAGGAGGTCATAAAGGATAGGATG
GAGAAGATAGGCCCCGAAATTGAATATCTTCGTAATAAATTGGCTGGTAAGAAGGTCTGGGTATTTTCAGGGGGTCCTAA
GAACTGGCACCTTCCACGCCCCCTTGAGGATGAACTTGGAATGGAGGTAGTTGCAGTTTCAACAATGTTCGAACACGAAG
ACGGCTATGAAAAAATCAAGAAAAGGGTCGGAGAAAATACGGTGATAGTTGATGACCCAAACTCACTGGAACTTGAGGAA
ATCATTGAAAAATACAGGCCAGATATCATCCTTTCAGGTATAAAGGAGAAGTATCTTGCCCATAAACTTGGTGTACCATG
CGTTCTCATCCATTCCTACGAAAATGGACCTTACATAGGTTTTGAGGGGTTCCTGAATCTCGCAAGGGACATGTATGCAT
CAATATACAGTCCTGTCTGGGATCTCCTTGAATTTGAGGCAGGTGATTGA

Upstream 100 bases:

>100_bases
TGCCCAGTGGATGATGCTGTAAGGATAAGAACCGGCGAGAATGGGGATGTGGCACTTCAATAGAGTGAATTGAATGGACT
TACTTGAAGGATGATTTAAA

Downstream 100 bases:

>100_bases
ATGTCAGAAATAAATGTGATGGAAAGAACAAGGGAACTTGTGGTAAACCCTCTTGTGACCTGTCAGCCATTCGGGGCAAT
GTTCGCAACCCTTGGCATAC

Product: nitrogenase NifD subunit

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MPFKLFDVDAEIPERKKHVYIKKKEDPEEDLPLCNTKTIPGCMTERGCAFAGAKGVITGAIKDALHVIHSPVGCTAYGYG
TKRYPTSQEMPDGSLFPIENFNLKYITGTNLTETDVVFGGMDKLKRCIIEAAKEFPEANAVYTYATCTTGLIGDDIEAVS
REVSEEIGKDVVAINAPGFAGPTQSKGHQVANYTLFENLVGTAEPPVVTDYDVNLIGEYNIDGDLWVLKRYFEEMGINVL
STFTGDCCHDEIKWMHRAKLSLVRCQRSATYIARLLEERYNVPYMKVDFFGIEYCKRNLMATGEYFGIPERAEEVIKDRM
EKIGPEIEYLRNKLAGKKVWVFSGGPKNWHLPRPLEDELGMEVVAVSTMFEHEDGYEKIKKRVGENTVIVDDPNSLELEE
IIEKYRPDIILSGIKEKYLAHKLGVPCVLIHSYENGPYIGFEGFLNLARDMYASIYSPVWDLLEFEAGD

Sequences:

>Translated_469_residues
MPFKLFDVDAEIPERKKHVYIKKKEDPEEDLPLCNTKTIPGCMTERGCAFAGAKGVITGAIKDALHVIHSPVGCTAYGYG
TKRYPTSQEMPDGSLFPIENFNLKYITGTNLTETDVVFGGMDKLKRCIIEAAKEFPEANAVYTYATCTTGLIGDDIEAVS
REVSEEIGKDVVAINAPGFAGPTQSKGHQVANYTLFENLVGTAEPPVVTDYDVNLIGEYNIDGDLWVLKRYFEEMGINVL
STFTGDCCHDEIKWMHRAKLSLVRCQRSATYIARLLEERYNVPYMKVDFFGIEYCKRNLMATGEYFGIPERAEEVIKDRM
EKIGPEIEYLRNKLAGKKVWVFSGGPKNWHLPRPLEDELGMEVVAVSTMFEHEDGYEKIKKRVGENTVIVDDPNSLELEE
IIEKYRPDIILSGIKEKYLAHKLGVPCVLIHSYENGPYIGFEGFLNLARDMYASIYSPVWDLLEFEAGD
>Mature_468_residues
PFKLFDVDAEIPERKKHVYIKKKEDPEEDLPLCNTKTIPGCMTERGCAFAGAKGVITGAIKDALHVIHSPVGCTAYGYGT
KRYPTSQEMPDGSLFPIENFNLKYITGTNLTETDVVFGGMDKLKRCIIEAAKEFPEANAVYTYATCTTGLIGDDIEAVSR
EVSEEIGKDVVAINAPGFAGPTQSKGHQVANYTLFENLVGTAEPPVVTDYDVNLIGEYNIDGDLWVLKRYFEEMGINVLS
TFTGDCCHDEIKWMHRAKLSLVRCQRSATYIARLLEERYNVPYMKVDFFGIEYCKRNLMATGEYFGIPERAEEVIKDRME
KIGPEIEYLRNKLAGKKVWVFSGGPKNWHLPRPLEDELGMEVVAVSTMFEHEDGYEKIKKRVGENTVIVDDPNSLELEEI
IEKYRPDIILSGIKEKYLAHKLGVPCVLIHSYENGPYIGFEGFLNLARDMYASIYSPVWDLLEFEAGD

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFD_METTH (O27605)

Other databases:

- EMBL:   AE000666
- PIR:   F69075
- RefSeq:   NP_276676.1
- ProteinModelPortal:   O27605
- SMR:   O27605
- STRING:   O27605
- GeneID:   1471832
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1563
- NMPDR:   fig|187420.1.peg.1531
- eggNOG:   arNOG04892
- HOGENOM:   HBG392742
- OMA:   FAGPSQS
- ProtClustDB:   CLSK878514
- BioCyc:   MTHE187420:MTH1563-MONOMER
- BRENDA:   1.18.6.1
- InterPro:   IPR000510
- InterPro:   IPR005974
- InterPro:   IPR010143
- InterPro:   IPR000318
- TIGRFAMs:   TIGR01284
- TIGRFAMs:   TIGR01862

Pfam domain/function: PF00148 Oxidored_nitro

EC number: =1.18.6.1

Molecular weight: Translated: 52867; Mature: 52736

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKLFDVDAEIPERKKHVYIKKKEDPEEDLPLCNTKTIPGCMTERGCAFAGAKGVITGA
CCCEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
IKDALHVIHSPVGCTAYGYGTKRYPTSQEMPDGSLFPIENFNLKYITGTNLTETDVVFGG
HHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHEECC
MDKLKRCIIEAAKEFPEANAVYTYATCTTGLIGDDIEAVSREVSEEIGKDVVAINAPGFA
HHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCC
GPTQSKGHQVANYTLFENLVGTAEPPVVTDYDVNLIGEYNIDGDLWVLKRYFEEMGINVL
CCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHCCHHH
STFTGDCCHDEIKWMHRAKLSLVRCQRSATYIARLLEERYNVPYMKVDFFGIEYCKRNLM
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
ATGEYFGIPERAEEVIKDRMEKIGPEIEYLRNKLAGKKVWVFSGGPKNWHLPRPLEDELG
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHC
MEVVAVSTMFEHEDGYEKIKKRVGENTVIVDDPNSLELEEIIEKYRPDIILSGIKEKYLA
CEEEEHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCEEHHHHHHHHH
HKLGVPCVLIHSYENGPYIGFEGFLNLARDMYASIYSPVWDLLEFEAGD
HHCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PFKLFDVDAEIPERKKHVYIKKKEDPEEDLPLCNTKTIPGCMTERGCAFAGAKGVITGA
CCEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
IKDALHVIHSPVGCTAYGYGTKRYPTSQEMPDGSLFPIENFNLKYITGTNLTETDVVFGG
HHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHEECC
MDKLKRCIIEAAKEFPEANAVYTYATCTTGLIGDDIEAVSREVSEEIGKDVVAINAPGFA
HHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCC
GPTQSKGHQVANYTLFENLVGTAEPPVVTDYDVNLIGEYNIDGDLWVLKRYFEEMGINVL
CCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHCCHHH
STFTGDCCHDEIKWMHRAKLSLVRCQRSATYIARLLEERYNVPYMKVDFFGIEYCKRNLM
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
ATGEYFGIPERAEEVIKDRMEKIGPEIEYLRNKLAGKKVWVFSGGPKNWHLPRPLEDELG
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHC
MEVVAVSTMFEHEDGYEKIKKRVGENTVIVDDPNSLELEEIIEKYRPDIILSGIKEKYLA
CEEEEHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCEEHHHHHHHHH
HKLGVPCVLIHSYENGPYIGFEGFLNLARDMYASIYSPVWDLLEFEAGD
HHCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463