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The map label for this gene is gatA

Identifier: 15679493

GI number: 15679493

Start: 1351797

End: 1353161

Strand: Direct

Name: gatA

Synonym: MTH1496

Alternate gene names: 15679493

Gene position: 1351797-1353161 (Clockwise)

Preceding gene: 15679487

Following gene: 15679494

Centisome position: 77.18

GC content: 51.72

Gene sequence:

>1365_bases
GTGTTAAAGATGGAAACTGTTGATAAGGTTGATATGATTAAGAATCATGGCCTGACCGCATCAGAAAATCTTGAAAAATT
TCTTAAGAGGATTGAGGCAAAAAACGATGACATCAACGCCTTCCTGGAGGTTAGGGGTGAAGAGGCGATTAAGAGGGCTG
AGGAGATAGACGCCAGGATAGCCTCCGGCAAGGAGACAGGGAAACTGGCGGGCCTGGTGATAGGAGTTAAAAGCAACATC
AACGTGGAGGACTTCAACGTATCAGCAGCTTCAAAGACCCTCGAAAATTACACTGGAAGCTACGATGCAACTGTTATAAG
GCGCATAAAGGAAGAAGACGGTATAATAATTGGCATGACCAACATGGACGAATTCGCTGCCGGGAGTTCAACCGAGACAT
CATTCTTTGGCCCAACAGACAATCCAGCAGCCCCCGGGAGGATCCCGGGAGGTTCAAGCGGCGGGAGCGCTGCTGCAGTG
GCTGCTGGTATGTGTGACCTGGCACTGGGATCAGATACAGGGGGTTCAATAAGGAATCCAGCATCGCACTGCGGTGTAAT
GGGCTTCAAACCAACCTATGGGGCAGTATCCAGGCAGGGCCTCCTGGACCTTGCCATGAGCTTTGACCAGATAGGACCCC
TGGCAGCAGATGTATCCGGGATCTCACTTGCACTGGATGTCATCTCAGGTTATGACCCGGCAGACCCCACAACCCTGGAC
AGTTCACCGGACCTTGAGGTGGAGAGGGAACTGAAGGGCCTCAGGGTCGGTGTTGTGAGGGAGTTCCTTGAGGTCACAGA
TGAGGCAATAGATGAGGTTATTCAGGGGAAACTCGGAGCCATGGAGGATGAGGGCGCCGAGATAGTTGAACTGGATTTTG
GATACATAGACCTCTGTCTCCCCACCTACTACCTCATAAACTACGTTGAATTCTTTTCAGCCACCAGGAAGTATGACGGC
AGAAAGTACGGTCACAGGATAGAGGATGTCTGCGGTTCGGAGGTCCTCAGGAGGATACATATGGGTTCCTACATCAGTCA
GAAGGAACTATCTGGAAAATACTACAAGAGGGCCCTCCAGGCAAGATCCCTCATAAGGAGGGAGATAACAGGGCTTCTCA
GCCATGTGGACATAATAGCGGGGCCAACGGTTCCAAAGCTACCCCACACACTGGGTGAGGAGCTTGAACCAATGGAGATG
TACGCCTACGATGTCCTCACGGTCATAGCTAACCTTGCAGGTATACCCGCTGCAAGCATACCTGCAGGGGATGTGGGCGG
AGTCCCTGTTGGCCTGCAGCTCCAGGCAAAGCCAGGAGATGATGGCATGATAGTATCGGCCATGAGGGAGATTGCTTCAC
TGTAA

Upstream 100 bases:

>100_bases
GTCCCGGCGGTCCATGGACCGGAAACTCCACCCTCGAGGCAGTCATGGCAGCTATTAAGGTTGATCAGACTTATATCTCA
CTTCAACTTGAAATATAAGG

Downstream 100 bases:

>100_bases
GAGAGTGTCCCCCATGAGGATTGGACTCGTCTGTATCAGGGGATCGGTCCCTGCCTTTGAGAACTTTGGATTTCTCCCCA
CCGATATAGTTGGAAGCAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MLKMETVDKVDMIKNHGLTASENLEKFLKRIEAKNDDINAFLEVRGEEAIKRAEEIDARIASGKETGKLAGLVIGVKSNI
NVEDFNVSAASKTLENYTGSYDATVIRRIKEEDGIIIGMTNMDEFAAGSSTETSFFGPTDNPAAPGRIPGGSSGGSAAAV
AAGMCDLALGSDTGGSIRNPASHCGVMGFKPTYGAVSRQGLLDLAMSFDQIGPLAADVSGISLALDVISGYDPADPTTLD
SSPDLEVERELKGLRVGVVREFLEVTDEAIDEVIQGKLGAMEDEGAEIVELDFGYIDLCLPTYYLINYVEFFSATRKYDG
RKYGHRIEDVCGSEVLRRIHMGSYISQKELSGKYYKRALQARSLIRREITGLLSHVDIIAGPTVPKLPHTLGEELEPMEM
YAYDVLTVIANLAGIPAASIPAGDVGGVPVGLQLQAKPGDDGMIVSAMREIASL

Sequences:

>Translated_454_residues
MLKMETVDKVDMIKNHGLTASENLEKFLKRIEAKNDDINAFLEVRGEEAIKRAEEIDARIASGKETGKLAGLVIGVKSNI
NVEDFNVSAASKTLENYTGSYDATVIRRIKEEDGIIIGMTNMDEFAAGSSTETSFFGPTDNPAAPGRIPGGSSGGSAAAV
AAGMCDLALGSDTGGSIRNPASHCGVMGFKPTYGAVSRQGLLDLAMSFDQIGPLAADVSGISLALDVISGYDPADPTTLD
SSPDLEVERELKGLRVGVVREFLEVTDEAIDEVIQGKLGAMEDEGAEIVELDFGYIDLCLPTYYLINYVEFFSATRKYDG
RKYGHRIEDVCGSEVLRRIHMGSYISQKELSGKYYKRALQARSLIRREITGLLSHVDIIAGPTVPKLPHTLGEELEPMEM
YAYDVLTVIANLAGIPAASIPAGDVGGVPVGLQLQAKPGDDGMIVSAMREIASL
>Mature_454_residues
MLKMETVDKVDMIKNHGLTASENLEKFLKRIEAKNDDINAFLEVRGEEAIKRAEEIDARIASGKETGKLAGLVIGVKSNI
NVEDFNVSAASKTLENYTGSYDATVIRRIKEEDGIIIGMTNMDEFAAGSSTETSFFGPTDNPAAPGRIPGGSSGGSAAAV
AAGMCDLALGSDTGGSIRNPASHCGVMGFKPTYGAVSRQGLLDLAMSFDQIGPLAADVSGISLALDVISGYDPADPTTLD
SSPDLEVERELKGLRVGVVREFLEVTDEAIDEVIQGKLGAMEDEGAEIVELDFGYIDLCLPTYYLINYVEFFSATRKYDG
RKYGHRIEDVCGSEVLRRIHMGSYISQKELSGKYYKRALQARSLIRREITGLLSHVDIIAGPTVPKLPHTLGEELEPMEM
YAYDVLTVIANLAGIPAASIPAGDVGGVPVGLQLQAKPGDDGMIVSAMREIASL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=488, Percent_Identity=33.1967213114754, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=22.7459016393443, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=464, Percent_Identity=34.2672413793103, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17537465, Length=471, Percent_Identity=25.0530785562633, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=31.7708333333333, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=31.7708333333333, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=29.1666666666667, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=220, Percent_Identity=28.1818181818182, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=32.8703703703704, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6319685, Length=442, Percent_Identity=28.2805429864253, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=220, Percent_Identity=28.6363636363636, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648113, Length=451, Percent_Identity=33.7028824833703, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24648435, Length=492, Percent_Identity=26.8292682926829, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24648437, Length=492, Percent_Identity=26.8292682926829, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI45550774, Length=492, Percent_Identity=26.8292682926829, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24652985, Length=476, Percent_Identity=25.2100840336134, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922090, Length=476, Percent_Identity=25.2100840336134, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652981, Length=476, Percent_Identity=25.2100840336134, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652983, Length=476, Percent_Identity=25.2100840336134, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648441, Length=426, Percent_Identity=26.056338028169, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648439, Length=426, Percent_Identity=26.056338028169, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24644968, Length=484, Percent_Identity=24.5867768595041, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161078093, Length=482, Percent_Identity=25.5186721991701, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21356731, Length=200, Percent_Identity=32.5, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_METTH (O27540)

Other databases:

- EMBL:   AE000666
- PIR:   D69066
- RefSeq:   NP_276610.1
- ProteinModelPortal:   O27540
- SMR:   O27540
- STRING:   O27540
- GeneID:   1471765
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1496
- NMPDR:   fig|187420.1.peg.1465
- eggNOG:   arNOG04872
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   MTHE187420:MTH1496-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 48582; Mature: 48582

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKMETVDKVDMIKNHGLTASENLEKFLKRIEAKNDDINAFLEVRGEEAIKRAEEIDARI
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHEECCHHHHHHHHHHHHHH
ASGKETGKLAGLVIGVKSNINVEDFNVSAASKTLENYTGSYDATVIRRIKEEDGIIIGMT
HCCCCCCCEEEEEEEECCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC
NMDEFAAGSSTETSFFGPTDNPAAPGRIPGGSSGGSAAAVAAGMCDLALGSDTGGSIRNP
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCC
ASHCGVMGFKPTYGAVSRQGLLDLAMSFDQIGPLAADVSGISLALDVISGYDPADPTTLD
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCCCCCC
SSPDLEVERELKGLRVGVVREFLEVTDEAIDEVIQGKLGAMEDEGAEIVELDFGYIDLCL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHH
PTYYLINYVEFFSATRKYDGRKYGHRIEDVCGSEVLRRIHMGSYISQKELSGKYYKRALQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHH
ARSLIRREITGLLSHVDIIAGPTVPKLPHTLGEELEPMEMYAYDVLTVIANLAGIPAASI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PAGDVGGVPVGLQLQAKPGDDGMIVSAMREIASL
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLKMETVDKVDMIKNHGLTASENLEKFLKRIEAKNDDINAFLEVRGEEAIKRAEEIDARI
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHEECCHHHHHHHHHHHHHH
ASGKETGKLAGLVIGVKSNINVEDFNVSAASKTLENYTGSYDATVIRRIKEEDGIIIGMT
HCCCCCCCEEEEEEEECCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC
NMDEFAAGSSTETSFFGPTDNPAAPGRIPGGSSGGSAAAVAAGMCDLALGSDTGGSIRNP
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCC
ASHCGVMGFKPTYGAVSRQGLLDLAMSFDQIGPLAADVSGISLALDVISGYDPADPTTLD
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCCCCCC
SSPDLEVERELKGLRVGVVREFLEVTDEAIDEVIQGKLGAMEDEGAEIVELDFGYIDLCL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHH
PTYYLINYVEFFSATRKYDGRKYGHRIEDVCGSEVLRRIHMGSYISQKELSGKYYKRALQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHH
ARSLIRREITGLLSHVDIIAGPTVPKLPHTLGEELEPMEMYAYDVLTVIANLAGIPAASI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PAGDVGGVPVGLQLQAKPGDDGMIVSAMREIASL
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463