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Identifier: 15679293

GI number: 15679293

Start: 1170071

End: 1170595

Strand: Direct

Name: Not Available

Synonym: MTH1289

Alternate gene names: 15679293

Gene position: 1170071-1170595 (Clockwise)

Preceding gene: 15679291

Following gene: 15679294

Centisome position: 66.81

GC content: 46.1

Gene sequence:

>525_bases
ATGTGCGAAGATAAAAAAACTGATTCTAGGTCCCAACAGGAATGCCAGAAGGAACTTGAGGAACTCAGGGAGCGTCTTAA
AGATTTAGAGAACGAAATCAAAAAGAAGGAAGAGGAAGTCAGGGAGTACACCTCCCACCTCCAGAGACTCCAGGCAGATT
TCGATAACTACAAGAAACAGATGGAGAAACAGGAACTCGAAATCATAAAAAACGCCAACGAAAGGCTCATACTCAAGCTC
CTGGATGTCTATGAGGACCTCGAAAGGGCCATTGAAAATCAGGACAGCAGCATGGATGGTCTTGAGGTCATCTACCGGAA
GTTCAGAGACACCCTCACAAAGGAGGGTCTCAGTGAGATACCTGCAGAGGGGGAGAAATTCGACCCCTTCCTCCACGAGG
CCGTTATGGTTGAGGACCATGACGGATATGAGGATGGTATCATCATCGAGGAGTTATCACGTGGTTACAGACTGAACGAC
CGCATAATAAAGCACTCAATTGTTAAGGTATGTAAGAAAAGCTGA

Upstream 100 bases:

>100_bases
ATGTCGAAAGGTTTATATAACCTTTTGTAAGTATAATAGCTATAAGGTTATAGGTAACCTCCGGTAACTTTAATTTCTGT
AAAAAATCAAAGGTGAACTG

Downstream 100 bases:

>100_bases
TTTGAGGTGATCACATGGCAAAGAAAGAAAAAATTCTCGGTATTGACCTTGGAACAAGTAACTCTGCAGCCGCAGTTCTC
ATAGGCGGTAAACCAACCAT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MCEDKKTDSRSQQECQKELEELRERLKDLENEIKKKEEEVREYTSHLQRLQADFDNYKKQMEKQELEIIKNANERLILKL
LDVYEDLERAIENQDSSMDGLEVIYRKFRDTLTKEGLSEIPAEGEKFDPFLHEAVMVEDHDGYEDGIIIEELSRGYRLND
RIIKHSIVKVCKKS

Sequences:

>Translated_174_residues
MCEDKKTDSRSQQECQKELEELRERLKDLENEIKKKEEEVREYTSHLQRLQADFDNYKKQMEKQELEIIKNANERLILKL
LDVYEDLERAIENQDSSMDGLEVIYRKFRDTLTKEGLSEIPAEGEKFDPFLHEAVMVEDHDGYEDGIIIEELSRGYRLND
RIIKHSIVKVCKKS
>Mature_174_residues
MCEDKKTDSRSQQECQKELEELRERLKDLENEIKKKEEEVREYTSHLQRLQADFDNYKKQMEKQELEIIKNANERLILKL
LDVYEDLERAIENQDSSMDGLEVIYRKFRDTLTKEGLSEIPAEGEKFDPFLHEAVMVEDHDGYEDGIIIEELSRGYRLND
RIIKHSIVKVCKKS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=156, Percent_Identity=29.4871794871795, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_METTH (O27350)

Other databases:

- EMBL:   AE000666
- PIR:   E69038
- RefSeq:   NP_276410.1
- ProteinModelPortal:   O27350
- SMR:   O27350
- STRING:   O27350
- GeneID:   1471697
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1289
- NMPDR:   fig|187420.1.peg.1265
- eggNOG:   arNOG11319
- HOGENOM:   HBG732630
- OMA:   FKTANQE
- ProtClustDB:   CLSK798127
- BioCyc:   MTHE187420:MTH1289-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20741; Mature: 20741

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCEDKKTDSRSQQECQKELEELRERLKDLENEIKKKEEEVREYTSHLQRLQADFDNYKKQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEKQELEIIKNANERLILKLLDVYEDLERAIENQDSSMDGLEVIYRKFRDTLTKEGLSEI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PAEGEKFDPFLHEAVMVEDHDGYEDGIIIEELSRGYRLNDRIIKHSIVKVCKKS
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCEDKKTDSRSQQECQKELEELRERLKDLENEIKKKEEEVREYTSHLQRLQADFDNYKKQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEKQELEIIKNANERLILKLLDVYEDLERAIENQDSSMDGLEVIYRKFRDTLTKEGLSEI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PAEGEKFDPFLHEAVMVEDHDGYEDGIIIEELSRGYRLNDRIIKHSIVKVCKKS
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463