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The map label for this gene is hemC

Identifier: 15678894

GI number: 15678894

Start: 795208

End: 796077

Strand: Direct

Name: hemC

Synonym: MTH874

Alternate gene names: 15678894

Gene position: 795208-796077 (Clockwise)

Preceding gene: 15678893

Following gene: 15678895

Centisome position: 45.4

GC content: 52.64

Gene sequence:

>870_bases
TTGATAGCCGGAACAAGGGGGAGTCGCCTGGCGCTGGTCCAGACGAACCATGTCATCGAGATGCTCAGTGAAGTCTGCAA
AGAGAAAATCGAGAAGAAGATCATAAAGACAAAGGGCGACAGGATAAGGGATTCCCAGCTCTACAGCATGGACTCAAGGG
GTCTATTCACAAGGGAACTCGACATGGCGGTCCTGAATGAGGAGGTGGACCTCGCAGTCCACAGCCTGAAGGACGTCCCC
AGCGACCTTGACCCTGACCTTGCCATCGCAGCCGTGCCCCCAAGGGAATCACCTGCAGAGGTCCTGGTATCCCGCCTTGA
CTGGGAAGATCTCCCCCAGGGTTCCAAACTTGGAACCAGCAGTCTACGCAGGGAAGCATTCTGCAACCATCACCAAAAAA
ATTTTAAGATGGAGCCCCTAAGAGGAAACATAGATACAAGGATAAGGAAGGTTATGGATGGCGAGGTCCATGCAACCATA
ATGGCCGAGGCAGGATTAAAACGTCTCGGACTGGAGGAGCACATAAAAAGAAGGTTCCCTGTGGAATACTTCACACCTGC
CGCCGGTCAGGGAGCCCTCGCAGTTATAACCAGGGCTGACAGTGAATTGATAAGCTCCATTGGGAGGATAACCCACCATC
CCTCCCTGCAGGAGGTCACCGCTGAGAAGACTCTCCTCAGAGAGCTCGGTGCAGGTTGCCAGTGCCCCCTGGGTGTCATA
GGAAGGGCCACTGGAAACCAGCTAACCCTCTACGCTGTTCTTCTAACCAGAGAAGGTGAAATGCTCAGGAAGGTAACCGT
TAGGGGGCCTCTTGCTGAAGCAGAGGATATAGGTAAAAAAGCTGCCAAAGAAATGGAGGATTATATTTGA

Upstream 100 bases:

>100_bases
CCATAATTATGTGATTTTTAATAAACTGGAGTTTTTGAAAAGTTATATAATAGGATACAATAAATTTCTCAAATGATTAA
CGTTTACTGGAGATGATTCA

Downstream 100 bases:

>100_bases
GACAGATAAATGTGGGTGTAATCGGCGTTGGAGCCATGGGTTACAACCATGCCAGAGTTTACTACAGACTTAAAAATGCA
AATCTTATGGCTGTATCAGA

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MIAGTRGSRLALVQTNHVIEMLSEVCKEKIEKKIIKTKGDRIRDSQLYSMDSRGLFTRELDMAVLNEEVDLAVHSLKDVP
SDLDPDLAIAAVPPRESPAEVLVSRLDWEDLPQGSKLGTSSLRREAFCNHHQKNFKMEPLRGNIDTRIRKVMDGEVHATI
MAEAGLKRLGLEEHIKRRFPVEYFTPAAGQGALAVITRADSELISSIGRITHHPSLQEVTAEKTLLRELGAGCQCPLGVI
GRATGNQLTLYAVLLTREGEMLRKVTVRGPLAEAEDIGKKAAKEMEDYI

Sequences:

>Translated_289_residues
MIAGTRGSRLALVQTNHVIEMLSEVCKEKIEKKIIKTKGDRIRDSQLYSMDSRGLFTRELDMAVLNEEVDLAVHSLKDVP
SDLDPDLAIAAVPPRESPAEVLVSRLDWEDLPQGSKLGTSSLRREAFCNHHQKNFKMEPLRGNIDTRIRKVMDGEVHATI
MAEAGLKRLGLEEHIKRRFPVEYFTPAAGQGALAVITRADSELISSIGRITHHPSLQEVTAEKTLLRELGAGCQCPLGVI
GRATGNQLTLYAVLLTREGEMLRKVTVRGPLAEAEDIGKKAAKEMEDYI
>Mature_289_residues
MIAGTRGSRLALVQTNHVIEMLSEVCKEKIEKKIIKTKGDRIRDSQLYSMDSRGLFTRELDMAVLNEEVDLAVHSLKDVP
SDLDPDLAIAAVPPRESPAEVLVSRLDWEDLPQGSKLGTSSLRREAFCNHHQKNFKMEPLRGNIDTRIRKVMDGEVHATI
MAEAGLKRLGLEEHIKRRFPVEYFTPAAGQGALAVITRADSELISSIGRITHHPSLQEVTAEKTLLRELGAGCQCPLGVI
GRATGNQLTLYAVLLTREGEMLRKVTVRGPLAEAEDIGKKAAKEMEDYI

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=264, Percent_Identity=36.7424242424242, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI20149500, Length=264, Percent_Identity=36.7424242424242, Blast_Score=146, Evalue=2e-35,
Organism=Escherichia coli, GI48994974, Length=288, Percent_Identity=36.8055555555556, Blast_Score=163, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319996, Length=271, Percent_Identity=33.9483394833948, Blast_Score=142, Evalue=4e-35,
Organism=Drosophila melanogaster, GI20130425, Length=255, Percent_Identity=37.2549019607843, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_METTH (O26960)

Other databases:

- EMBL:   AE000666
- PIR:   G69216
- RefSeq:   NP_276011.1
- ProteinModelPortal:   O26960
- SMR:   O26960
- STRING:   O26960
- GeneID:   1471282
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH874
- NMPDR:   fig|187420.1.peg.866
- eggNOG:   arNOG08614
- HOGENOM:   HBG624842
- OMA:   RATEVFD
- ProtClustDB:   PRK00072
- BioCyc:   MTHE187420:MTH874-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 32026; Mature: 32026

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGTRGSRLALVQTNHVIEMLSEVCKEKIEKKIIKTKGDRIRDSQLYSMDSRGLFTREL
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHH
DMAVLNEEVDLAVHSLKDVPSDLDPDLAIAAVPPRESPAEVLVSRLDWEDLPQGSKLGTS
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCHHHCCCCCCCCHH
SLRREAFCNHHQKNFKMEPLRGNIDTRIRKVMDGEVHATIMAEAGLKRLGLEEHIKRRFP
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHEEHHHHHHHHHCCHHHHHHHHCC
VEYFTPAAGQGALAVITRADSELISSIGRITHHPSLQEVTAEKTLLRELGAGCQCPLGVI
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
GRATGNQLTLYAVLLTREGEMLRKVTVRGPLAEAEDIGKKAAKEMEDYI
CCCCCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAGTRGSRLALVQTNHVIEMLSEVCKEKIEKKIIKTKGDRIRDSQLYSMDSRGLFTREL
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHH
DMAVLNEEVDLAVHSLKDVPSDLDPDLAIAAVPPRESPAEVLVSRLDWEDLPQGSKLGTS
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCHHHCCCCCCCCHH
SLRREAFCNHHQKNFKMEPLRGNIDTRIRKVMDGEVHATIMAEAGLKRLGLEEHIKRRFP
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHEEHHHHHHHHHCCHHHHHHHHCC
VEYFTPAAGQGALAVITRADSELISSIGRITHHPSLQEVTAEKTLLRELGAGCQCPLGVI
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
GRATGNQLTLYAVLLTREGEMLRKVTVRGPLAEAEDIGKKAAKEMEDYI
CCCCCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463