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The map label for this gene is ppnK

Identifier: 15678892

GI number: 15678892

Start: 792918

End: 793769

Strand: Direct

Name: ppnK

Synonym: MTH872

Alternate gene names: 15678892

Gene position: 792918-793769 (Clockwise)

Preceding gene: 15678891

Following gene: 15678893

Centisome position: 45.27

GC content: 50.7

Gene sequence:

>852_bases
ATGATGCGCATAGGGATAATAGCCCGTTTTGATGTTGCAGAGGCCGTTGAAATCGCTGAAAGAGTGGCCTCATTCCTCCT
GAACAGGGGGGTTGAAATTACGGTTGACCTCAAGCTCACAGAGGAACTCCCACAGCTGAGGGAATACGGTGAGGACATAA
GGAACATGGATGCAGACATGATACTCACCATCGGCGGGGATGGGACAATACTCAGGACCCGGAGCCTCATAGAGGATAAG
GAGATACCTATACTCGGTATAAACATGGGGACAGTTGGTTTTTTAACTGAGGTTGACCCTGAAAATGTATTCTCAGCCCT
TGAAGCTGTCCTCAGGGGCGAATACGCCGTTGAGAAGAGGACCCTCCTGAGTGTCTACCACAATGATGAGCTGCCCTCCG
CCCTCAACGAGGTCGTTCTCATGACCCGTAGGCCAGCGAAGATGCTGCATATAGAGATATCTGTTGATGATGAGGTGGTT
GAGGAGCTACGCGCCGACGGGATAATCATAGCAACGCCAAGTGGCTCAACAGCGTACTCGATGTCAGCAGGGGGCCCCAT
TGTCGACCCCAGGGTTGAAGCGTTCCTCATAGTGCCAATATGTCCCTTCAAACTCAGCGCAAGACCCCTTGTGGTTTCAA
ACAAGAGTGTAATCCGGGTTAAACTCCTCAGGAAGGGTAAAAAGGCCATCGCCGTTATCGACGGCCAGTATGAGGAGGAG
ATCAACCACATGGATGAGGTCATATTCAGGAAGTCAGAACGAAAGGCCCACTTCGTGAGGCTCAGCAAGGATTTCTACAG
GAAGGTCCGTGAAAAACTCATAGAGGGCGGAATAGACTCTATAAAGGGTTAA

Upstream 100 bases:

>100_bases
GAGAAATAGACGGCCTTCTGAACGTTAAGGCAAGAACATCCCTTGTTGCCGCCGGAAACCTGGAGCTCCACGAGAAGATA
ATGCAGACCCTGGAGGTCAT

Downstream 100 bases:

>100_bases
AGGTTGTGTCCATGAAATTTAACATTTCAAGGCCCCTCGTGACTGACCTCACACATGGCGGTGTCACCATTGCCCTGGAG
ATGAGGAAACACTTCCCTGA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MMRIGIIARFDVAEAVEIAERVASFLLNRGVEITVDLKLTEELPQLREYGEDIRNMDADMILTIGGDGTILRTRSLIEDK
EIPILGINMGTVGFLTEVDPENVFSALEAVLRGEYAVEKRTLLSVYHNDELPSALNEVVLMTRRPAKMLHIEISVDDEVV
EELRADGIIIATPSGSTAYSMSAGGPIVDPRVEAFLIVPICPFKLSARPLVVSNKSVIRVKLLRKGKKAIAVIDGQYEEE
INHMDEVIFRKSERKAHFVRLSKDFYRKVREKLIEGGIDSIKG

Sequences:

>Translated_283_residues
MMRIGIIARFDVAEAVEIAERVASFLLNRGVEITVDLKLTEELPQLREYGEDIRNMDADMILTIGGDGTILRTRSLIEDK
EIPILGINMGTVGFLTEVDPENVFSALEAVLRGEYAVEKRTLLSVYHNDELPSALNEVVLMTRRPAKMLHIEISVDDEVV
EELRADGIIIATPSGSTAYSMSAGGPIVDPRVEAFLIVPICPFKLSARPLVVSNKSVIRVKLLRKGKKAIAVIDGQYEEE
INHMDEVIFRKSERKAHFVRLSKDFYRKVREKLIEGGIDSIKG
>Mature_283_residues
MMRIGIIARFDVAEAVEIAERVASFLLNRGVEITVDLKLTEELPQLREYGEDIRNMDADMILTIGGDGTILRTRSLIEDK
EIPILGINMGTVGFLTEVDPENVFSALEAVLRGEYAVEKRTLLSVYHNDELPSALNEVVLMTRRPAKMLHIEISVDDEVV
EELRADGIIIATPSGSTAYSMSAGGPIVDPRVEAFLIVPICPFKLSARPLVVSNKSVIRVKLLRKGKKAIAVIDGQYEEE
INHMDEVIFRKSERKAHFVRLSKDFYRKVREKLIEGGIDSIKG

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=235, Percent_Identity=32.3404255319149, Blast_Score=98, Evalue=7e-21,
Organism=Escherichia coli, GI1788968, Length=271, Percent_Identity=35.4243542435424, Blast_Score=159, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325068, Length=259, Percent_Identity=32.4324324324324, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6320794, Length=238, Percent_Identity=30.2521008403361, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322509, Length=230, Percent_Identity=33.0434782608696, Blast_Score=105, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28573828, Length=250, Percent_Identity=28.8, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28573830, Length=250, Percent_Identity=28.8, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573826, Length=250, Percent_Identity=28.8, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161077047, Length=214, Percent_Identity=31.3084112149533, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573832, Length=214, Percent_Identity=31.3084112149533, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653424, Length=261, Percent_Identity=28.735632183908, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129957, Length=261, Percent_Identity=28.735632183908, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653422, Length=261, Percent_Identity=28.735632183908, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281363323, Length=261, Percent_Identity=28.735632183908, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281363321, Length=261, Percent_Identity=28.735632183908, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_METTH (O26958)

Other databases:

- EMBL:   AE000666
- PIR:   E69216
- RefSeq:   NP_276009.1
- ProteinModelPortal:   O26958
- SMR:   O26958
- STRING:   O26958
- GeneID:   1471280
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH872
- NMPDR:   fig|187420.1.peg.864
- eggNOG:   arNOG04399
- HOGENOM:   HBG713904
- OMA:   DPRLDVI
- PhylomeDB:   O26958
- ProtClustDB:   PRK03708
- BioCyc:   MTHE187420:MTH872-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 31604; Mature: 31604

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIGIIARFDVAEAVEIAERVASFLLNRGVEITVDLKLTEELPQLREYGEDIRNMDADM
CEEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCE
ILTIGGDGTILRTRSLIEDKEIPILGINMGTVGFLTEVDPENVFSALEAVLRGEYAVEKR
EEEECCCCCEEEEHHHHCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHH
TLLSVYHNDELPSALNEVVLMTRRPAKMLHIEISVDDEVVEELRADGIIIATPSGSTAYS
HHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCEEEECCCCCCEEE
MSAGGPIVDPRVEAFLIVPICPFKLSARPLVVSNKSVIRVKLLRKGKKAIAVIDGQYEEE
ECCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCEEEEEEHHCCCCEEEEECCCHHHH
INHMDEVIFRKSERKAHFVRLSKDFYRKVREKLIEGGIDSIKG
HHHHHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MMRIGIIARFDVAEAVEIAERVASFLLNRGVEITVDLKLTEELPQLREYGEDIRNMDADM
CEEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCE
ILTIGGDGTILRTRSLIEDKEIPILGINMGTVGFLTEVDPENVFSALEAVLRGEYAVEKR
EEEECCCCCEEEEHHHHCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHH
TLLSVYHNDELPSALNEVVLMTRRPAKMLHIEISVDDEVVEELRADGIIIATPSGSTAYS
HHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCEEEECCCCCCEEE
MSAGGPIVDPRVEAFLIVPICPFKLSARPLVVSNKSVIRVKLLRKGKKAIAVIDGQYEEE
ECCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCEEEEEEHHCCCCEEEEECCCHHHH
INHMDEVIFRKSERKAHFVRLSKDFYRKVREKLIEGGIDSIKG
HHHHHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463