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Identifier: 15678524

GI number: 15678524

Start: 436138

End: 436665

Strand: Direct

Name: Not Available

Synonym: MTH496

Alternate gene names: 15678524

Gene position: 436138-436665 (Clockwise)

Preceding gene: 15678523

Following gene: 15678525

Centisome position: 24.9

GC content: 42.05

Gene sequence:

>528_bases
ATGCTTCACAGAACACGCGCAGAACAGGTGCTTGAAATTTATGAAAACTTTGTTGAAAAATTTCCTGATTTTAAATCGGT
GTGTGAAGCGGGACAGGAAACAATAGAAAAAGAAATGGAGTCCCTTGGTCTCAGATGGAGAGCCAGAAATCTCCATAAAC
TCGCATGTGAGATTGAAAGCAGACACGGGGGGGCTGTTCCAAAAAACAAAAATGATCTCCTTGAACTTCCAGGCATTGGG
AACTACATTTCCTCGGCGTTTTTATGTTTTTCCAAAAATATTCCAGAACCCCTTCTGGACACGAACACCGTCAGAATCAT
TGGTAGATTGTTTGATCTTGAGATCAGTGATTCATCCCGCAGAAAAAAGGATTTTGAAACAGTAATGAGGAAAATTCTTG
AATTTGGTGACTGCAGACACCTTTCACTATCGATGATCGATTTCGGAGAGGCTGTGTGCAGAGCTAGCGATCCCCTCTGC
CATGAATGTCCCCTGAAACTATCATGCAACTTTTACAGGAGATGTTAG

Upstream 100 bases:

>100_bases
TGATATTCATACCAGAATCTGCGAATGGGGAAAAAAGAATTTAAGGAAATTTCCCTGGCGCGAAAGAAGAGATGACTATG
GAATTCTGCTGGCTGAAATA

Downstream 100 bases:

>100_bases
AATGAGAAAAACAATGATTGAAGAGGTAATAAAGGAGGTGCTGGGACCACGAAGTGGCCCTGAGGAATCCATTAAAGGGG
ACCCCTACGATGAGTATATA

Product: endonuclease III-like protein

Products: NA

Alternate protein names: Mismatch glycosylase; MIG; Thymine-DNA glycosylase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MLHRTRAEQVLEIYENFVEKFPDFKSVCEAGQETIEKEMESLGLRWRARNLHKLACEIESRHGGAVPKNKNDLLELPGIG
NYISSAFLCFSKNIPEPLLDTNTVRIIGRLFDLEISDSSRRKKDFETVMRKILEFGDCRHLSLSMIDFGEAVCRASDPLC
HECPLKLSCNFYRRC

Sequences:

>Translated_175_residues
MLHRTRAEQVLEIYENFVEKFPDFKSVCEAGQETIEKEMESLGLRWRARNLHKLACEIESRHGGAVPKNKNDLLELPGIG
NYISSAFLCFSKNIPEPLLDTNTVRIIGRLFDLEISDSSRRKKDFETVMRKILEFGDCRHLSLSMIDFGEAVCRASDPLC
HECPLKLSCNFYRRC
>Mature_175_residues
MLHRTRAEQVLEIYENFVEKFPDFKSVCEAGQETIEKEMESLGLRWRARNLHKLACEIESRHGGAVPKNKNDLLELPGIG
NYISSAFLCFSKNIPEPLLDTNTVRIIGRLFDLEISDSSRRKKDFETVMRKILEFGDCRHLSLSMIDFGEAVCRASDPLC
HECPLKLSCNFYRRC

Specific function: Could act in DNA repair. Restriction methylase M.mthtI, which is encoded by this plasmid, generates 5-methylcytosine which is, especially under thermophilic conditions, subject to deamination resulting in G-T mismatches. This protein could correct these m

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298650, Length=176, Percent_Identity=23.2954545454545, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI115298654, Length=176, Percent_Identity=23.2954545454545, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI115298652, Length=176, Percent_Identity=23.2954545454545, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI115298648, Length=176, Percent_Identity=23.2954545454545, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI6912520, Length=176, Percent_Identity=23.2954545454545, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI190358497, Length=176, Percent_Identity=23.2954545454545, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1789331, Length=170, Percent_Identity=27.6470588235294, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 20188; Mature: 20188

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
5.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHRTRAEQVLEIYENFVEKFPDFKSVCEAGQETIEKEMESLGLRWRARNLHKLACEIES
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RHGGAVPKNKNDLLELPGIGNYISSAFLCFSKNIPEPLLDTNTVRIIGRLFDLEISDSSR
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHCCCCCCHH
RKKDFETVMRKILEFGDCRHLSLSMIDFGEAVCRASDPLCHECPLKLSCNFYRRC
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MLHRTRAEQVLEIYENFVEKFPDFKSVCEAGQETIEKEMESLGLRWRARNLHKLACEIES
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RHGGAVPKNKNDLLELPGIGNYISSAFLCFSKNIPEPLLDTNTVRIIGRLFDLEISDSSR
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHCCCCCCHH
RKKDFETVMRKILEFGDCRHLSLSMIDFGEAVCRASDPLCHECPLKLSCNFYRRC
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1336177; 11786018 [H]