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Identifier: 15678442

GI number: 15678442

Start: 350625

End: 352034

Strand: Direct

Name: Not Available

Synonym: MTH414

Alternate gene names: 15678442

Gene position: 350625-352034 (Clockwise)

Preceding gene: 15678441

Following gene: 15678443

Centisome position: 20.02

GC content: 57.09

Gene sequence:

>1410_bases
GTGACCGCGATGTGCGCCATTGCAGGGTTCAGGGGTGATGACGCCCCGGTAAAGGTACAGGAGATGTTAGATGTCCTCAG
ACACCGGGGCCCTGATGCCACAGGGATCTACCATGACAGCAGGATAGTGATAAGGACAGCGACGGGGGAGGATGTAAAGG
AGGCCCCGCAATCAGCTGAGATTGCAGTGGGCCATAACCTCCTCTCAATCGTGGGAGGTCCTCAGCCAGTATCAGGGGAC
GGCGTACTGGTATTTAACGGCGAGATATACAATCAGGAGGAACTTAACTGGACCGGCAGCGACTCTGAACTCATCCTGGA
CCTCATAGAGGGCCACGGCGGGGACCTGGAGGATGCGCTGCGTTTCACGGTTAACAAGATAGACGGTGACTACGCCTTCG
CCTACACAGACGGCGAGAACCTTGCAAGTCGTCAGGACCCTGTTGGTGTCAAACCCCTCTACCATTCAGGGGAGGCCTTC
GCATCCGAAAAGAAGGCCCTCTGGAGGATAGGTCTGAGGAATGTGGAGACCCTGCCACCTGGCCATGCCATGATAAACGG
TGTGAAGGTGAAGCTGAGGGGACTCCCCAGGGCGGAACCCTCAGATGCAGAACCAGAAGAGCTGAGGGAGAATCTCAAAT
CAGCTCTGAGGGAATCGGTGGAGAGGAGAGTTAGAGGTTTGGATGAGGCTGCCCTGGTATTCTCGGGCGGCGTTGACAGC
ACACTGCTGGCGGTCCTCCTTGATGAACACATCGATGTGAGGCTCTACACCGTGGGGCTCCCTGGTTCCAGCGACCCTCA
GTTCGCATCCAGGGCTGCGGCAGACCTTGGAATGGAACTTGAGGTCCTGGAGGTTACAGAGGATACCATCAGGGAGGCCC
TTCCCCATGTCCTGGGGGCGATAGAGGAGTACAATCTCATGAAGATCGGCGTTGCAATGCCCCTCTACCTGGCATCTGAG
GCTGCCTCTGCAGACGGATACAGGGTCATGTTCTCTGGTCAGGGTGCAGACGAACTTTTCGCAGGATACCACAGGTACAG
GCGCCTCCTGGCTGAGGGAAGACTCGAGGACGCCCTCCGGCATGACCTTGAAAACATCCACCATGTTAACCTTGAAAGGG
ATGACGCGGTTACAATGGCAAACTCGGTGGAGCTGAGGGTCCCCTTCCTGGACCTTCAACTGATAGGGGTGGCCCTGACC
ATACCAGCAGACCTCAAGATACGGGGCCCGGAGGATGAGCTGAGGAAACGCATACTCAGGGAGGCGGCCCTTGAGATGGG
GGTCCCCGAGTACATTGCGATGAGGCCCAAGAAGGCGGCGCAGTACGGTTCAGGGATAGATAAGGTCCTGCGGAGGAAGG
TTCTCCCTGGGTTTGACCATGAGACCTTCCTGAGGAGGCTGATGTTTTAA

Upstream 100 bases:

>100_bases
GAACTTGAACCAAAATCAGCCATACTCATGACCGTGGAGGAGATATACTCAGTAAAACCGGGGCCAGAGGCCGGTGAAAA
GGTGGCCTGAGCTCACAGAA

Downstream 100 bases:

>100_bases
CCCATGTCTGCAGTGGTCATCCTGTCACACAGGATCCATGTTCCCTCTGCTGAATCTGAGGCCATGTTTTTTCTCTCTGT
GACAGGTCTCTGTGGCAAGG

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTAMCAIAGFRGDDAPVKVQEMLDVLRHRGPDATGIYHDSRIVIRTATGEDVKEAPQSAEIAVGHNLLSIVGGPQPVSGD
GVLVFNGEIYNQEELNWTGSDSELILDLIEGHGGDLEDALRFTVNKIDGDYAFAYTDGENLASRQDPVGVKPLYHSGEAF
ASEKKALWRIGLRNVETLPPGHAMINGVKVKLRGLPRAEPSDAEPEELRENLKSALRESVERRVRGLDEAALVFSGGVDS
TLLAVLLDEHIDVRLYTVGLPGSSDPQFASRAAADLGMELEVLEVTEDTIREALPHVLGAIEEYNLMKIGVAMPLYLASE
AASADGYRVMFSGQGADELFAGYHRYRRLLAEGRLEDALRHDLENIHHVNLERDDAVTMANSVELRVPFLDLQLIGVALT
IPADLKIRGPEDELRKRILREAALEMGVPEYIAMRPKKAAQYGSGIDKVLRRKVLPGFDHETFLRRLMF

Sequences:

>Translated_469_residues
MTAMCAIAGFRGDDAPVKVQEMLDVLRHRGPDATGIYHDSRIVIRTATGEDVKEAPQSAEIAVGHNLLSIVGGPQPVSGD
GVLVFNGEIYNQEELNWTGSDSELILDLIEGHGGDLEDALRFTVNKIDGDYAFAYTDGENLASRQDPVGVKPLYHSGEAF
ASEKKALWRIGLRNVETLPPGHAMINGVKVKLRGLPRAEPSDAEPEELRENLKSALRESVERRVRGLDEAALVFSGGVDS
TLLAVLLDEHIDVRLYTVGLPGSSDPQFASRAAADLGMELEVLEVTEDTIREALPHVLGAIEEYNLMKIGVAMPLYLASE
AASADGYRVMFSGQGADELFAGYHRYRRLLAEGRLEDALRHDLENIHHVNLERDDAVTMANSVELRVPFLDLQLIGVALT
IPADLKIRGPEDELRKRILREAALEMGVPEYIAMRPKKAAQYGSGIDKVLRRKVLPGFDHETFLRRLMF
>Mature_468_residues
TAMCAIAGFRGDDAPVKVQEMLDVLRHRGPDATGIYHDSRIVIRTATGEDVKEAPQSAEIAVGHNLLSIVGGPQPVSGDG
VLVFNGEIYNQEELNWTGSDSELILDLIEGHGGDLEDALRFTVNKIDGDYAFAYTDGENLASRQDPVGVKPLYHSGEAFA
SEKKALWRIGLRNVETLPPGHAMINGVKVKLRGLPRAEPSDAEPEELRENLKSALRESVERRVRGLDEAALVFSGGVDST
LLAVLLDEHIDVRLYTVGLPGSSDPQFASRAAADLGMELEVLEVTEDTIREALPHVLGAIEEYNLMKIGVAMPLYLASEA
ASADGYRVMFSGQGADELFAGYHRYRRLLAEGRLEDALRHDLENIHHVNLERDDAVTMANSVELRVPFLDLQLIGVALTI
PADLKIRGPEDELRKRILREAALEMGVPEYIAMRPKKAAQYGSGIDKVLRRKVLPGFDHETFLRRLMF

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=504, Percent_Identity=27.3809523809524, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI168229252, Length=504, Percent_Identity=27.3809523809524, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI168229250, Length=504, Percent_Identity=27.3809523809524, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI296010848, Length=481, Percent_Identity=27.2349272349272, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI296010852, Length=395, Percent_Identity=27.8481012658228, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI296010850, Length=395, Percent_Identity=27.8481012658228, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1786889, Length=491, Percent_Identity=28.9205702647658, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17560178, Length=466, Percent_Identity=27.2532188841202, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25147557, Length=470, Percent_Identity=28.2978723404255, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71993933, Length=468, Percent_Identity=28.8461538461538, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321563, Length=518, Percent_Identity=27.2200772200772, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325403, Length=523, Percent_Identity=26.3862332695985, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323542, Length=458, Percent_Identity=25.3275109170306, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45553209, Length=473, Percent_Identity=30.0211416490486, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 51588; Mature: 51457

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAMCAIAGFRGDDAPVKVQEMLDVLRHRGPDATGIYHDSRIVIRTATGEDVKEAPQSAE
CCCEEEEECCCCCCCCEEHHHHHHHHHHCCCCCCCEEECCEEEEEECCCCHHHHCCCCCE
IAVGHNLLSIVGGPQPVSGDGVLVFNGEIYNQEELNWTGSDSELILDLIEGHGGDLEDAL
EEECCHHHHHCCCCCCCCCCEEEEECCCEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
RFTVNKIDGDYAFAYTDGENLASRQDPVGVKPLYHSGEAFASEKKALWRIGLRNVETLPP
HHHHHCCCCCEEEEEECCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
GHAMINGVKVKLRGLPRAEPSDAEPEELRENLKSALRESVERRVRGLDEAALVFSGGVDS
CHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
TLLAVLLDEHIDVRLYTVGLPGSSDPQFASRAAADLGMELEVLEVTEDTIREALPHVLGA
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHH
IEEYNLMKIGVAMPLYLASEAASADGYRVMFSGQGADELFAGYHRYRRLLAEGRLEDALR
HHHCCEEEEHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHH
HDLENIHHVNLERDDAVTMANSVELRVPFLDLQLIGVALTIPADLKIRGPEDELRKRILR
HHHHHHEECCCCCCCCEEEECCEEEEECEEEEEEEEEEEECCCCEEEECCHHHHHHHHHH
EAALEMGVPEYIAMRPKKAAQYGSGIDKVLRRKVLPGFDHETFLRRLMF
HHHHHCCCCHHHHCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TAMCAIAGFRGDDAPVKVQEMLDVLRHRGPDATGIYHDSRIVIRTATGEDVKEAPQSAE
CCEEEEECCCCCCCCEEHHHHHHHHHHCCCCCCCEEECCEEEEEECCCCHHHHCCCCCE
IAVGHNLLSIVGGPQPVSGDGVLVFNGEIYNQEELNWTGSDSELILDLIEGHGGDLEDAL
EEECCHHHHHCCCCCCCCCCEEEEECCCEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
RFTVNKIDGDYAFAYTDGENLASRQDPVGVKPLYHSGEAFASEKKALWRIGLRNVETLPP
HHHHHCCCCCEEEEEECCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
GHAMINGVKVKLRGLPRAEPSDAEPEELRENLKSALRESVERRVRGLDEAALVFSGGVDS
CHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
TLLAVLLDEHIDVRLYTVGLPGSSDPQFASRAAADLGMELEVLEVTEDTIREALPHVLGA
HHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHH
IEEYNLMKIGVAMPLYLASEAASADGYRVMFSGQGADELFAGYHRYRRLLAEGRLEDALR
HHHCCEEEEHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHH
HDLENIHHVNLERDDAVTMANSVELRVPFLDLQLIGVALTIPADLKIRGPEDELRKRILR
HHHHHHEECCCCCCCCEEEECCEEEEECEEEEEEEEEEEECCCCEEEECCHHHHHHHHHH
EAALEMGVPEYIAMRPKKAAQYGSGIDKVLRRKVLPGFDHETFLRRLMF
HHHHHCCCCHHHHCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]