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Identifier: 15678433

GI number: 15678433

Start: 342980

End: 343765

Strand: Direct

Name: Not Available

Synonym: MTH405

Alternate gene names: 15678433

Gene position: 342980-343765 (Clockwise)

Preceding gene: 15678432

Following gene: 15678434

Centisome position: 19.58

GC content: 51.78

Gene sequence:

>786_bases
TTGAAAGGATGCAGGGGTTGTATCATGGATATCATCTTTAAGAAGAAACTGGAGGACCTCCGGAGGGATGTTGCACTAAA
GTCGATGGACCTTGAGGAGAGACCTGAGGACGTTGATGTGGAACTTGCAAGCTGCAGGGTTCTGGACAAATTCATAGACA
TAACACCGAAGTGTGTAAGGTGCAATCTCTGTTTTGAGGAGTGCCCTGTGGACGCCATATCAGAATCATCAGCATCAAAA
CCTGCAAGGATACTTGATAACTGTGTTAAATGTGAGATATGCGCCCAGACCTGCCCTGTGAGGTGCATAAACGTTGTTGA
GAGCACCGCCACCATCGGTGACGAGGACGTCACCTACAACCTGGAATACGTCAGGATCCCCCACCGCCTGCTCCGGATGA
AGAACATAGAGGTCACAGATAGATGCACTGCATGCGGGACCTGCACCAGGTTCTGCCCCACAGGTGCAATTCAGTTAGAT
AAGGAGATCGCGGTGGTCGATGAATCCATCTGCATTGGCTGCGGGGCCTGCGTCAACGTCTGCCCCTCAGATGCGGTGGA
ACTTGAGAGGGAACTGGGCCCTGTGATTGAGACCCGGCGGCTCCTGGTTGACCAGGATGCCTGCGTCGAGTGCCTTGTCT
GTGAGGAGAACTGTCCCACCGGGGCCATAAGGATTGAGGATGGAGAGGTTGTGGTTGATAAGGATAAATGCATTCTATGT
GAGGTTTGTTCCACAAGGTGTCCTGTGGCAGCACTGAAACTGGAGAGGTTGGCAGATGAAAGTTAA

Upstream 100 bases:

>100_bases
ATCTGGGTGTGCTGAGGCCTGATGTTGATCCCGTGAACCGGAAAAAAATTTAAGTAAATACGTTTAAAAGGTAATAAAGG
GTTGTGTTAACTCTTGAAGC

Downstream 100 bases:

>100_bases
GGAGATTATGGATAAGGAGTTTATAGCAGTCTCCCCTGAGGATCGGGTTGTTGATGTCTCATTGAAGATGGAGGAGACAC
GTAAGTTCACAACACCTGTT

Product: polyferredoxin

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKGCRGCIMDIIFKKKLEDLRRDVALKSMDLEERPEDVDVELASCRVLDKFIDITPKCVRCNLCFEECPVDAISESSASK
PARILDNCVKCEICAQTCPVRCINVVESTATIGDEDVTYNLEYVRIPHRLLRMKNIEVTDRCTACGTCTRFCPTGAIQLD
KEIAVVDESICIGCGACVNVCPSDAVELERELGPVIETRRLLVDQDACVECLVCEENCPTGAIRIEDGEVVVDKDKCILC
EVCSTRCPVAALKLERLADES

Sequences:

>Translated_261_residues
MKGCRGCIMDIIFKKKLEDLRRDVALKSMDLEERPEDVDVELASCRVLDKFIDITPKCVRCNLCFEECPVDAISESSASK
PARILDNCVKCEICAQTCPVRCINVVESTATIGDEDVTYNLEYVRIPHRLLRMKNIEVTDRCTACGTCTRFCPTGAIQLD
KEIAVVDESICIGCGACVNVCPSDAVELERELGPVIETRRLLVDQDACVECLVCEENCPTGAIRIEDGEVVVDKDKCILC
EVCSTRCPVAALKLERLADES
>Mature_261_residues
MKGCRGCIMDIIFKKKLEDLRRDVALKSMDLEERPEDVDVELASCRVLDKFIDITPKCVRCNLCFEECPVDAISESSASK
PARILDNCVKCEICAQTCPVRCINVVESTATIGDEDVTYNLEYVRIPHRLLRMKNIEVTDRCTACGTCTRFCPTGAIQLD
KEIAVVDESICIGCGACVNVCPSDAVELERELGPVIETRRLLVDQDACVECLVCEENCPTGAIRIEDGEVVVDKDKCILC
EVCSTRCPVAALKLERLADES

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 28882; Mature: 28882

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
12.3 %Cys+Met (Translated Protein)
10.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
12.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGCRGCIMDIIFKKKLEDLRRDVALKSMDLEERPEDVDVELASCRVLDKFIDITPKCVR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEHHHHHHHHHHHCCCCCEEE
CNLCFEECPVDAISESSASKPARILDNCVKCEICAQTCPVRCINVVESTATIGDEDVTYN
EEECHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEE
LEYVRIPHRLLRMKNIEVTDRCTACGTCTRFCPTGAIQLDKEIAVVDESICIGCGACVNV
EEEEEHHHHHHHHCCCCHHHCCCCCCCHHHHCCCCCEEECCCCEEECCHHHEECCHHHHC
CPSDAVELERELGPVIETRRLLVDQDACVECLVCEENCPTGAIRIEDGEVVVDKDKCILC
CCCHHHHHHHHHCHHHHHHHHHCCCCHHEEEEEECCCCCCCEEEEECCCEEEECCCEEEE
EVCSTRCPVAALKLERLADES
EHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKGCRGCIMDIIFKKKLEDLRRDVALKSMDLEERPEDVDVELASCRVLDKFIDITPKCVR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEHHHHHHHHHHHCCCCCEEE
CNLCFEECPVDAISESSASKPARILDNCVKCEICAQTCPVRCINVVESTATIGDEDVTYN
EEECHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEE
LEYVRIPHRLLRMKNIEVTDRCTACGTCTRFCPTGAIQLDKEIAVVDESICIGCGACVNV
EEEEEHHHHHHHHCCCCHHHCCCCCCCHHHHCCCCCEEECCCCEEECCHHHEECCHHHHC
CPSDAVELERELGPVIETRRLLVDQDACVECLVCEENCPTGAIRIEDGEVVVDKDKCILC
CCCHHHHHHHHHCHHHHHHHHHCCCCHHEEEEEECCCCCCCEEEEECCCEEEECCCEEEE
EVCSTRCPVAALKLERLADES
EHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]