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Identifier: 15678427

GI number: 15678427

Start: 337628

End: 338653

Strand: Direct

Name: Not Available

Synonym: MTH399

Alternate gene names: 15678427

Gene position: 337628-338653 (Clockwise)

Preceding gene: 15678426

Following gene: 15678428

Centisome position: 19.28

GC content: 53.12

Gene sequence:

>1026_bases
ATGTCCTCTGTAATATGGTACCTTTACGAGTTTGCCCGTAAGTCCTGGGCAGAGAAATTCGCCAATGCACACACAGAACA
CGAGATCCTTGAAAAACCAGAGAGATTCAGGGATTTCCCCACAGTTAAGAGGGAGTACTGCATAGGCTGTGGGGCCTGCA
CAACGGCCTGTCCTGCCCCTGGCGCAATAAAACTGGTCCGTGACACCGACACGGCCGAGGAGGAGGGTCAGACCTACCCT
GTCATAGTCAGGGGTGCGTGCATCAGGTGCGGCTTCTGCGCCGAGGTCTGCCCCACCGACCCCAAGACAATTGAATGCGG
AGAGAATCACCTCATAAGGGAAGAGTTCACCATAGTACCCTCAGAGAAGCTCTATGTCATAGATGACTACCTCTGCATAC
GCTGCAAGAAGTGCATGAAGGCCTGCCCGGTGGATGCAATAACCGAGAAGGACGGCAGGGTCGAGGTTGACCAGGGCAGG
TGCATAGCCTGCGGTGAATGCCTCGAGAAGTGTCCTGTCAAGGGCGCCCTCAAGGTGATACACGTCGCCTACGTCGAGGA
ACAGAAGATGGTCATAAACCTGGCGGTCAATGAACTGGAATCAGCCATAGAGGAGAAGAGCGAGGATATAAAGAAACTCG
AAGCCGAAGGTGTCTACAGGATGAATTACCCCCTGAAACCCCTCCTCGAGAGGGCCCTTGAGATCCTCCCGGATGAGGAG
ATAGTCAGGGACCTCCTCGAGAAGATAACCGACCGCCTCAAGATGCGCATCATAACATGGAGCCCCGAAAAATGTGTGCA
GTGCCGTTTATGCGTGGATGAATGCCCATCAGGAGCCATAACCTACTCAGAGGATGAAGGAATCGTGAGGGACCCTGATA
AGTGTCTCAGGTGCAGCACATGCTACCAGACATGCCCCTTCGGTGTTGCAGGGTACTACGTTGCAAGGTTCCTCATAGAT
GAATCCAATGGAGAAGAGATGATAAGGATAACCATAAAACCGGCGGCTCTTCCTGTAAAGCGATGA

Upstream 100 bases:

>100_bases
CTGATCCATGCATAGCATGCGCAGAGAGGGTCATGGTTGTTGATGAAGAGAGTGGGGAGAGGGAGATACTCCTCTAATAA
ATGCGTGATGTTGATGTCCT

Downstream 100 bases:

>100_bases
GGAGATGATAAAATTCCAGCTGCAGCAAAACCCGTGAAGCCCCTGAGGGAAGTTGACGTTGACTATGATATTGACAGTGA
GAAGTGCAGGGAATGCCCTG

Product: polyferredoxin

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSSVIWYLYEFARKSWAEKFANAHTEHEILEKPERFRDFPTVKREYCIGCGACTTACPAPGAIKLVRDTDTAEEEGQTYP
VIVRGACIRCGFCAEVCPTDPKTIECGENHLIREEFTIVPSEKLYVIDDYLCIRCKKCMKACPVDAITEKDGRVEVDQGR
CIACGECLEKCPVKGALKVIHVAYVEEQKMVINLAVNELESAIEEKSEDIKKLEAEGVYRMNYPLKPLLERALEILPDEE
IVRDLLEKITDRLKMRIITWSPEKCVQCRLCVDECPSGAITYSEDEGIVRDPDKCLRCSTCYQTCPFGVAGYYVARFLID
ESNGEEMIRITIKPAALPVKR

Sequences:

>Translated_341_residues
MSSVIWYLYEFARKSWAEKFANAHTEHEILEKPERFRDFPTVKREYCIGCGACTTACPAPGAIKLVRDTDTAEEEGQTYP
VIVRGACIRCGFCAEVCPTDPKTIECGENHLIREEFTIVPSEKLYVIDDYLCIRCKKCMKACPVDAITEKDGRVEVDQGR
CIACGECLEKCPVKGALKVIHVAYVEEQKMVINLAVNELESAIEEKSEDIKKLEAEGVYRMNYPLKPLLERALEILPDEE
IVRDLLEKITDRLKMRIITWSPEKCVQCRLCVDECPSGAITYSEDEGIVRDPDKCLRCSTCYQTCPFGVAGYYVARFLID
ESNGEEMIRITIKPAALPVKR
>Mature_340_residues
SSVIWYLYEFARKSWAEKFANAHTEHEILEKPERFRDFPTVKREYCIGCGACTTACPAPGAIKLVRDTDTAEEEGQTYPV
IVRGACIRCGFCAEVCPTDPKTIECGENHLIREEFTIVPSEKLYVIDDYLCIRCKKCMKACPVDAITEKDGRVEVDQGRC
IACGECLEKCPVKGALKVIHVAYVEEQKMVINLAVNELESAIEEKSEDIKKLEAEGVYRMNYPLKPLLERALEILPDEEI
VRDLLEKITDRLKMRIITWSPEKCVQCRLCVDECPSGAITYSEDEGIVRDPDKCLRCSTCYQTCPFGVAGYYVARFLIDE
SNGEEMIRITIKPAALPVKR

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 8 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 38639; Mature: 38508

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
7.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVIWYLYEFARKSWAEKFANAHTEHEILEKPERFRDFPTVKREYCIGCGACTTACPAP
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCCHHHHHHCCCCCCCCCCCCC
GAIKLVRDTDTAEEEGQTYPVIVRGACIRCGFCAEVCPTDPKTIECGENHLIREEFTIVP
CCEEEEECCCCHHHCCCCCEEEEECCHHCCCCHHHCCCCCCCEEECCCCCEEHHHEEECC
SEKLYVIDDYLCIRCKKCMKACPVDAITEKDGRVEVDQGRCIACGECLEKCPVKGALKVI
CCCEEEECHHHHHHHHHHHHHCCCCHHCCCCCCEEECCCCEEEHHHHHHHCCCCHHHHHH
HVAYVEEQKMVINLAVNELESAIEEKSEDIKKLEAEGVYRMNYPLKPLLERALEILPDEE
HHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCHH
IVRDLLEKITDRLKMRIITWSPEKCVQCRLCVDECPSGAITYSEDEGIVRDPDKCLRCST
HHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHH
CYQTCPFGVAGYYVARFLIDESNGEEMIRITIKPAALPVKR
HHHHCCCCHHHHHHHHHHEECCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
SSVIWYLYEFARKSWAEKFANAHTEHEILEKPERFRDFPTVKREYCIGCGACTTACPAP
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCCHHHHHHCCCCCCCCCCCCC
GAIKLVRDTDTAEEEGQTYPVIVRGACIRCGFCAEVCPTDPKTIECGENHLIREEFTIVP
CCEEEEECCCCHHHCCCCCEEEEECCHHCCCCHHHCCCCCCCEEECCCCCEEHHHEEECC
SEKLYVIDDYLCIRCKKCMKACPVDAITEKDGRVEVDQGRCIACGECLEKCPVKGALKVI
CCCEEEECHHHHHHHHHHHHHCCCCHHCCCCCCEEECCCCEEEHHHHHHHCCCCHHHHHH
HVAYVEEQKMVINLAVNELESAIEEKSEDIKKLEAEGVYRMNYPLKPLLERALEILPDEE
HHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCHH
IVRDLLEKITDRLKMRIITWSPEKCVQCRLCVDECPSGAITYSEDEGIVRDPDKCLRCST
HHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHH
CYQTCPFGVAGYYVARFLIDESNGEEMIRITIKPAALPVKR
HHHHCCCCHHHHHHHHHHEECCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]