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Identifier: 15678278

GI number: 15678278

Start: 193838

End: 194350

Strand: Direct

Name: Not Available

Synonym: MTH250

Alternate gene names: 15678278

Gene position: 193838-194350 (Clockwise)

Preceding gene: 15678277

Following gene: 15678279

Centisome position: 11.07

GC content: 51.07

Gene sequence:

>513_bases
TTGAGAGTGGAGGGACAGCTCGGAGATGAGGTGGTCACCATAAAGGCCACATCCATCGCAAGGAGGCTTCATGGAAAGAG
CCACTACGGTAAGATGTATGAGGACAGACTTCAGCTTTCACTTATTGAGGCGGCCTACCTCATGGAGAGGGGTAAGCTCA
AACTCATGAAGGACGATGATGAGGTGTCCCCTGAGGAATTCATATCCCTCCTCGGTGAACGGGGACTCTACAGCAAGTAT
CTTGTCTACAGGGATCTCAGGAACAGGGGATACATAGTTAAAACAGGTTTCAAGTATGGTGCAGAGTTCAGACTATATGA
GAGGGGAGGGGCTCCCGGCAGGACCCACTCAGCCTACCTTGTGAGGGTGATCTCAGAGAATGACACCATCCACGCCCTTG
ACTTTTCAAGTTATGTGAGGGTGGCCCATGGGGTTAACAAGAAGCTCCTCATGGCCTTCCTCGACGATGAGGAGGACATA
ACCTACTACCTTGTCGACTGGATAAGGCCATGA

Upstream 100 bases:

>100_bases
GCCTCATAGCCGAGTTCATGTCAATCCTTGGAAAGAGGGAGAAGGACCTCAAGGAGAGGCACGCCATAATCGAGTAGCTC
AACCAGCACTCACGGGAGGT

Downstream 100 bases:

>100_bases
TTTGGCTACTATGACGGCTTAAGCAACAGAAATGACTTAATAACGGTGATATGATTGATAGACCCTTGGGGATCAGCAAA
GCTTGAATACCAGGATCTTA

Product: tRNA-splicing endonuclease subunit alpha

Products: NA

Alternate protein names: tRNA-intron endonuclease

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MRVEGQLGDEVVTIKATSIARRLHGKSHYGKMYEDRLQLSLIEAAYLMERGKLKLMKDDDEVSPEEFISLLGERGLYSKY
LVYRDLRNRGYIVKTGFKYGAEFRLYERGGAPGRTHSAYLVRVISENDTIHALDFSSYVRVAHGVNKKLLMAFLDDEEDI
TYYLVDWIRP

Sequences:

>Translated_170_residues
MRVEGQLGDEVVTIKATSIARRLHGKSHYGKMYEDRLQLSLIEAAYLMERGKLKLMKDDDEVSPEEFISLLGERGLYSKY
LVYRDLRNRGYIVKTGFKYGAEFRLYERGGAPGRTHSAYLVRVISENDTIHALDFSSYVRVAHGVNKKLLMAFLDDEEDI
TYYLVDWIRP
>Mature_170_residues
MRVEGQLGDEVVTIKATSIARRLHGKSHYGKMYEDRLQLSLIEAAYLMERGKLKLMKDDDEVSPEEFISLLGERGLYSKY
LVYRDLRNRGYIVKTGFKYGAEFRLYERGGAPGRTHSAYLVRVISENDTIHALDFSSYVRVAHGVNKKLLMAFLDDEEDI
TYYLVDWIRP

Specific function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate

COG id: COG1676

COG function: function code J; tRNA splicing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENDA_METTH (O07165)

Other databases:

- EMBL:   AF001577
- EMBL:   AE000666
- PIR:   D69131
- RefSeq:   NP_275393.1
- ProteinModelPortal:   O07165
- SMR:   O07165
- STRING:   O07165
- GeneID:   1470211
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH250
- NMPDR:   fig|187420.1.peg.248
- eggNOG:   arNOG05458
- HOGENOM:   HBG297289
- OMA:   DFRLYER
- ProtClustDB:   PRK09297
- BioCyc:   MTHE187420:MTH250-MONOMER
- BRENDA:   3.1.27.9
- HAMAP:   MF_01833
- InterPro:   IPR011856
- InterPro:   IPR006676
- InterPro:   IPR006677
- InterPro:   IPR006678
- InterPro:   IPR016442
- Gene3D:   G3DSA:3.40.1350.10
- PIRSF:   PIRSF005285
- TIGRFAMs:   TIGR00324

Pfam domain/function: PF01974 tRNA_int_endo; PF02778 tRNA_int_endo_N; SSF53032 tRNA_int_endo_C; SSF55267 tRNA_int_endo_N

EC number: =3.1.27.9

Molecular weight: Translated: 19693; Mature: 19693

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: ACT_SITE 106-106 ACT_SITE 116-116 ACT_SITE 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVEGQLGDEVVTIKATSIARRLHGKSHYGKMYEDRLQLSLIEAAYLMERGKLKLMKDDD
CCCCCCCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
EVSPEEFISLLGERGLYSKYLVYRDLRNRGYIVKTGFKYGAEFRLYERGGAPGRTHSAYL
CCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEE
VRVISENDTIHALDFSSYVRVAHGVNKKLLMAFLDDEEDITYYLVDWIRP
EEEECCCCEEEEECHHHHHHHHHCCCHHEEEEEECCCCCEEEEEEEECCC
>Mature Secondary Structure
MRVEGQLGDEVVTIKATSIARRLHGKSHYGKMYEDRLQLSLIEAAYLMERGKLKLMKDDD
CCCCCCCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
EVSPEEFISLLGERGLYSKYLVYRDLRNRGYIVKTGFKYGAEFRLYERGGAPGRTHSAYL
CCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEE
VRVISENDTIHALDFSSYVRVAHGVNKKLLMAFLDDEEDITYYLVDWIRP
EEEECCCCEEEEECHHHHHHHHHCCCHHEEEEEECCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9200602; 9371463