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The map label for this gene is aspC

Identifier: 15678254

GI number: 15678254

Start: 168672

End: 169985

Strand: Direct

Name: aspC

Synonym: MTH226

Alternate gene names: 15678254

Gene position: 168672-169985 (Clockwise)

Preceding gene: 15678253

Following gene: 15678255

Centisome position: 9.63

GC content: 50.0

Gene sequence:

>1314_bases
TTGTTACTTGGAGATCTTAGAAGGACTCATTACTCAAAGGACATAGAACCGGAGATGGATGGAGATGAAGTCACGGTTAT
GGGCTGGGTCCATGAAATAAGGGACCTTGGAGGGATAATATTCGTACTCCTAAGGGACCGTGACGGGCTCATACAGATAA
CAGCCCCCAGTAAAAAGATTGAAAAGGACCTCTTCAAATCCATAAGGAAGCTCAAAAAGGAATCCGTGGTCGCCTTTGGT
GGTACTGTGCAGGAGTCAGGCAAGGCACCGGGCGGATTTGAGATAATACCCTCCTTCCTCAAGGTACTTAACATATCAAA
GCAGCCCCTCCCACTGGACCCAACAGAGAAGGTTAAGGCTGAGATAGACACAAGACTCGACGCAAGATTCCTTGACCTCA
GAAAACCATCCGTAAGCGCAATATTCAAGATAAAAAGCAGAATGCTGCATTCAGTCAGGGTATTCCTTGAAGAACAGGGA
TTCCTCGAGATAAACACCCCAAAGTTAGTTGCATCTGCAACAGAGGGAGGTACAGAGCTTTTCCCCATAACCTACTTTGA
GAGGGAGGCCTTCCTCGGCCAGAGCCCACAGCTGTACAAGCAGATCATGATGTCAACGGGCCTGGACCGTGTGTACGAAA
TCGCCCCAATATTCAGGGCAGAGGAACACGACACCCTCAGGCACCTCAACGAGGTCATATCAATCGACATCGAGGCATCC
TTTGTTGACCACGAGGACGTGATGAAGATACTTGAAAACCTCGTGGTGAGGGTCATTGAAGACGTGAATGAGCACTGCAC
TGATGCCCTGGAAACCCTTGGAAGGACACTGGAGGTGCCTGAAACACCATTCGAGCGCCTGGAGTATGATGAGGCCGTTG
AAATGGTTAACTCAAAGGGAGTTCCGATGAAACATGGAGAAGACCTCCCAAGAGCCGCTGAGAAGGCCCTGGGTGAGATC
ATGGATGGCTACTACTTCATAACATCCTGGCCAACCGCCATAAAACCATTCTATGTCATGCCAGACGAGGATGACCCTGA
GAGGAGTCATGCATTTGACCTCATGTACAGGGACCTTGAGATATCCTCAGGGGCCATGAGGGTACACCAGCATGACCTCC
TTGTTGAGAAGATAAAAAGACAGGGTCTTAACCCTGATTCATTTGAGAGCTACCTTTCAGCCTTTGAGTACGGCATGCCA
CCCCACGCAGGATGGGGACTCGGTGCTGAGAGGTTCAACATGACCCTCACAGGACTTAAAAACATAAGGGAGACGGTCCT
CTTCCCGAGGGACAGGAGGAGGCTCACGCCGTAG

Upstream 100 bases:

>100_bases
GGACTGATTGATGAAGGCAGATTAATCTGCTGAACTCTGATAATGCATGGTTTCATCAGAGACCTTAAATATTCACTTTA
AACTGTTAGAGGTGATTTGA

Downstream 100 bases:

>100_bases
GTGATAATTTGATGGGAGCATCCACAGGCCAGGGCTATGAAATAGCAAGGAAGAGCAGGGAGATAGTGAGGGAACTCATC
TCCGAGGATATATCCTCGCT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MLLGDLRRTHYSKDIEPEMDGDEVTVMGWVHEIRDLGGIIFVLLRDRDGLIQITAPSKKIEKDLFKSIRKLKKESVVAFG
GTVQESGKAPGGFEIIPSFLKVLNISKQPLPLDPTEKVKAEIDTRLDARFLDLRKPSVSAIFKIKSRMLHSVRVFLEEQG
FLEINTPKLVASATEGGTELFPITYFEREAFLGQSPQLYKQIMMSTGLDRVYEIAPIFRAEEHDTLRHLNEVISIDIEAS
FVDHEDVMKILENLVVRVIEDVNEHCTDALETLGRTLEVPETPFERLEYDEAVEMVNSKGVPMKHGEDLPRAAEKALGEI
MDGYYFITSWPTAIKPFYVMPDEDDPERSHAFDLMYRDLEISSGAMRVHQHDLLVEKIKRQGLNPDSFESYLSAFEYGMP
PHAGWGLGAERFNMTLTGLKNIRETVLFPRDRRRLTP

Sequences:

>Translated_437_residues
MLLGDLRRTHYSKDIEPEMDGDEVTVMGWVHEIRDLGGIIFVLLRDRDGLIQITAPSKKIEKDLFKSIRKLKKESVVAFG
GTVQESGKAPGGFEIIPSFLKVLNISKQPLPLDPTEKVKAEIDTRLDARFLDLRKPSVSAIFKIKSRMLHSVRVFLEEQG
FLEINTPKLVASATEGGTELFPITYFEREAFLGQSPQLYKQIMMSTGLDRVYEIAPIFRAEEHDTLRHLNEVISIDIEAS
FVDHEDVMKILENLVVRVIEDVNEHCTDALETLGRTLEVPETPFERLEYDEAVEMVNSKGVPMKHGEDLPRAAEKALGEI
MDGYYFITSWPTAIKPFYVMPDEDDPERSHAFDLMYRDLEISSGAMRVHQHDLLVEKIKRQGLNPDSFESYLSAFEYGMP
PHAGWGLGAERFNMTLTGLKNIRETVLFPRDRRRLTP
>Mature_437_residues
MLLGDLRRTHYSKDIEPEMDGDEVTVMGWVHEIRDLGGIIFVLLRDRDGLIQITAPSKKIEKDLFKSIRKLKKESVVAFG
GTVQESGKAPGGFEIIPSFLKVLNISKQPLPLDPTEKVKAEIDTRLDARFLDLRKPSVSAIFKIKSRMLHSVRVFLEEQG
FLEINTPKLVASATEGGTELFPITYFEREAFLGQSPQLYKQIMMSTGLDRVYEIAPIFRAEEHDTLRHLNEVISIDIEAS
FVDHEDVMKILENLVVRVIEDVNEHCTDALETLGRTLEVPETPFERLEYDEAVEMVNSKGVPMKHGEDLPRAAEKALGEI
MDGYYFITSWPTAIKPFYVMPDEDDPERSHAFDLMYRDLEISSGAMRVHQHDLLVEKIKRQGLNPDSFESYLSAFEYGMP
PHAGWGLGAERFNMTLTGLKNIRETVLFPRDRRRLTP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI45439306, Length=448, Percent_Identity=35.4910714285714, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI4758762, Length=428, Percent_Identity=29.2056074766355, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI226491502, Length=440, Percent_Identity=27.0454545454545, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI194272210, Length=469, Percent_Identity=28.997867803838, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI5031815, Length=469, Percent_Identity=28.997867803838, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI40789249, Length=263, Percent_Identity=34.9809885931559, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1787161, Length=450, Percent_Identity=27.3333333333333, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788173, Length=257, Percent_Identity=34.6303501945525, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1790571, Length=474, Percent_Identity=25.9493670886076, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789256, Length=473, Percent_Identity=25.5813953488372, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17551876, Length=461, Percent_Identity=33.6225596529284, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71984122, Length=427, Percent_Identity=30.6791569086651, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI71994340, Length=462, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17535927, Length=462, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17535925, Length=462, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17556372, Length=407, Percent_Identity=28.2555282555283, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566633, Length=301, Percent_Identity=29.5681063122924, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323011, Length=460, Percent_Identity=36.5217391304348, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321807, Length=432, Percent_Identity=32.8703703703704, Blast_Score=230, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6319872, Length=458, Percent_Identity=25.1091703056769, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320242, Length=458, Percent_Identity=24.235807860262, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325153, Length=258, Percent_Identity=30.6201550387597, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136276, Length=445, Percent_Identity=35.5056179775281, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI19921528, Length=425, Percent_Identity=31.2941176470588, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI19922416, Length=451, Percent_Identity=24.8337028824834, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24640851, Length=463, Percent_Identity=28.2937365010799, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24640849, Length=463, Percent_Identity=28.2937365010799, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24584738, Length=317, Percent_Identity=29.0220820189274, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYD_METTH (O26328)

Other databases:

- EMBL:   AE000666
- PIR:   A69128
- RefSeq:   NP_275369.1
- ProteinModelPortal:   O26328
- SMR:   O26328
- STRING:   O26328
- GeneID:   1470187
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH226
- NMPDR:   fig|187420.1.peg.224
- eggNOG:   arNOG04316
- HOGENOM:   HBG745843
- OMA:   ASAFPRD
- PhylomeDB:   O26328
- ProtClustDB:   PRK05159
- BioCyc:   MTHE187420:MTH226-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_A
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00458

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 49839; Mature: 49839

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGDLRRTHYSKDIEPEMDGDEVTVMGWVHEIRDLGGIIFVLLRDRDGLIQITAPSKKI
CCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEEECCCCEEEEECCHHHH
EKDLFKSIRKLKKESVVAFGGTVQESGKAPGGFEIIPSFLKVLNISKQPLPLDPTEKVKA
HHHHHHHHHHHHHHCEEEECCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
EIDTRLDARFLDLRKPSVSAIFKIKSRMLHSVRVFLEEQGFLEINTPKLVASATEGGTEL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCEE
FPITYFEREAFLGQSPQLYKQIMMSTGLDRVYEIAPIFRAEEHDTLRHLNEVISIDIEAS
EEEEEEHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEE
FVDHEDVMKILENLVVRVIEDVNEHCTDALETLGRTLEVPETPFERLEYDEAVEMVNSKG
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHCCC
VPMKHGEDLPRAAEKALGEIMDGYYFITSWPTAIKPFYVMPDEDDPERSHAFDLMYRDLE
CCCCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHCCEEECCCCCCCHHHHHHHHHHHHHC
ISSGAMRVHQHDLLVEKIKRQGLNPDSFESYLSAFEYGMPPHAGWGLGAERFNMTLTGLK
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHH
NIRETVLFPRDRRRLTP
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLLGDLRRTHYSKDIEPEMDGDEVTVMGWVHEIRDLGGIIFVLLRDRDGLIQITAPSKKI
CCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEEECCCCEEEEECCHHHH
EKDLFKSIRKLKKESVVAFGGTVQESGKAPGGFEIIPSFLKVLNISKQPLPLDPTEKVKA
HHHHHHHHHHHHHHCEEEECCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
EIDTRLDARFLDLRKPSVSAIFKIKSRMLHSVRVFLEEQGFLEINTPKLVASATEGGTEL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCEE
FPITYFEREAFLGQSPQLYKQIMMSTGLDRVYEIAPIFRAEEHDTLRHLNEVISIDIEAS
EEEEEEHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEE
FVDHEDVMKILENLVVRVIEDVNEHCTDALETLGRTLEVPETPFERLEYDEAVEMVNSKG
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHCCC
VPMKHGEDLPRAAEKALGEIMDGYYFITSWPTAIKPFYVMPDEDDPERSHAFDLMYRDLE
CCCCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHCCEEECCCCCCCHHHHHHHHHHHHHC
ISSGAMRVHQHDLLVEKIKRQGLNPDSFESYLSAFEYGMPPHAGWGLGAERFNMTLTGLK
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHH
NIRETVLFPRDRRRLTP
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463