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Identifier: 15678219

GI number: 15678219

Start: 134949

End: 135866

Strand: Direct

Name: Not Available

Synonym: MTH191

Alternate gene names: 15678219

Gene position: 134949-135866 (Clockwise)

Preceding gene: 15678218

Following gene: 15678220

Centisome position: 7.71

GC content: 49.13

Gene sequence:

>918_bases
TTGTGTGGAATAGCGGGAGTGGTCTACAAGGATGGTAAACTGCACTCAGTCGGGGCAGACATGACAAGGATGCTCCATGC
ACTGCAGCACAGGGGACCAGACTCAGCAGGATTCGCGATATATGGAGGTCTTGGGCTTGAAGAAAACGATTACCTCCTTA
ACATAGAGGTTAAGGATAAACCAGGACTTCTTGACATGGTTAAGGAAACAGTTGAACTTGTAAGCCCCATAGGATCAGAT
GAGGTCATACCATCAGTTGAGAACCACATAATCTACCGGTGCAGGATAACCCTGGAATCATTCTCACAGCTTAAACCACT
CATAATGGATATAGACAGCATAGATGACGTTATAGTACTGAACGGTAGCCACTCATTTGAGATGATAAAGGATGTGGGTT
CGGTTCTTGAGATAGCAGACCGCTACGACACCTGGTCAAAGAAGGGCACCCATGCCATAGGCCACACAAGGTTCTCAACA
GAAAGTATAGTTGACAGGTACCATGCCCACCCATTCCAGAGCTACATCATCCCCGACATAACCGTCGTCCACAATGGCCA
GATAACAAACTACTGGAAGATAAGGGAGCCGCTGGAGCGCAAGGGCCACATATTCGAAACAAACAACGACACAGAGTGCA
TAGTCCACTACGTGGCAGATAAACTGGCATCAGGTTACAGCCTCGAGGAAGCCCTTGAACAGTCAGTAAAGGACATGGAT
GGCCCCTTCTCATACATAGTGGGAACACCCCAGGGTGTTGGAATAGCCAAGGACCAGCTTGGACTGAGGCCAGGGGTCAT
GGCAGAGAATGATGAGGTCTTCGCAGTCGCATCAGAGGAGGTCTCCCTCAGGGAGGTCATGGACACAAGTGAGGTTGAAC
AGATATCCCCTGGAGAGGTAAGGGTCTACGAGATATGA

Upstream 100 bases:

>100_bases
TAATCGCAGTAAAGGACGGCTGCAACCTCGCACTGGCCTTTCACCCTGAACTCGGAGAGGACACAGGACTCCATGAATAC
TTTATAAAGGAGGTATTGAA

Downstream 100 bases:

>100_bases
GGGTGATACCATGAGGGAAGCGGTAATTGATGCAGGATCAAAAACCCCGAGGGAGGTTAACAGGGCAATAAAATCCCTTG
CAGCCGACCACGACAGGATA

Product: glutamine PRPP amidotransferase

Products: L-glutamine; 5-phospho-alpha-D-ribose 1-diphosphate; H2O

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MCGIAGVVYKDGKLHSVGADMTRMLHALQHRGPDSAGFAIYGGLGLEENDYLLNIEVKDKPGLLDMVKETVELVSPIGSD
EVIPSVENHIIYRCRITLESFSQLKPLIMDIDSIDDVIVLNGSHSFEMIKDVGSVLEIADRYDTWSKKGTHAIGHTRFST
ESIVDRYHAHPFQSYIIPDITVVHNGQITNYWKIREPLERKGHIFETNNDTECIVHYVADKLASGYSLEEALEQSVKDMD
GPFSYIVGTPQGVGIAKDQLGLRPGVMAENDEVFAVASEEVSLREVMDTSEVEQISPGEVRVYEI

Sequences:

>Translated_305_residues
MCGIAGVVYKDGKLHSVGADMTRMLHALQHRGPDSAGFAIYGGLGLEENDYLLNIEVKDKPGLLDMVKETVELVSPIGSD
EVIPSVENHIIYRCRITLESFSQLKPLIMDIDSIDDVIVLNGSHSFEMIKDVGSVLEIADRYDTWSKKGTHAIGHTRFST
ESIVDRYHAHPFQSYIIPDITVVHNGQITNYWKIREPLERKGHIFETNNDTECIVHYVADKLASGYSLEEALEQSVKDMD
GPFSYIVGTPQGVGIAKDQLGLRPGVMAENDEVFAVASEEVSLREVMDTSEVEQISPGEVRVYEI
>Mature_305_residues
MCGIAGVVYKDGKLHSVGADMTRMLHALQHRGPDSAGFAIYGGLGLEENDYLLNIEVKDKPGLLDMVKETVELVSPIGSD
EVIPSVENHIIYRCRITLESFSQLKPLIMDIDSIDDVIVLNGSHSFEMIKDVGSVLEIADRYDTWSKKGTHAIGHTRFST
ESIVDRYHAHPFQSYIIPDITVVHNGQITNYWKIREPLERKGHIFETNNDTECIVHYVADKLASGYSLEEALEQSVKDMD
GPFSYIVGTPQGVGIAKDQLGLRPGVMAENDEVFAVASEEVSLREVMDTSEVEQISPGEVRVYEI

Specific function: Catalyzes The First Step In Hexosamine Metabolism, Converting Fructose-6p Into Glucosamine-6p Using Glutamine As A Nitrogen Source. [C]

COG id: COG0067

COG function: function code E; Glutamate synthase domain 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Escherichia coli, GI1790167, Length=157, Percent_Identity=29.9363057324841, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788651, Length=201, Percent_Identity=27.363184079602, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17539970, Length=95, Percent_Identity=37.8947368421053, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17532897, Length=95, Percent_Identity=36.8421052631579, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17532899, Length=121, Percent_Identity=33.0578512396694, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21357745, Length=133, Percent_Identity=32.3308270676692, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y191_METTH (O26293)

Other databases:

- EMBL:   AE000666
- PIR:   A69122
- RefSeq:   NP_275334.1
- ProteinModelPortal:   O26293
- STRING:   O26293
- GeneID:   1470152
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH191
- NMPDR:   fig|187420.1.peg.189
- eggNOG:   arNOG04106
- HOGENOM:   HBG299832
- OMA:   HNGQITN
- ProtClustDB:   CLSK878444
- BioCyc:   MTHE187420:MTH191-MONOMER
- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- PANTHER:   PTHR11907

Pfam domain/function: PF00310 GATase_2

EC number: 2.4.2.14

Molecular weight: Translated: 33911; Mature: 33911

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS51278 GATASE_TYPE_2

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGVVYKDGKLHSVGADMTRMLHALQHRGPDSAGFAIYGGLGLEENDYLLNIEVKDK
CCCCCEEEEECCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCC
PGLLDMVKETVELVSPIGSDEVIPSVENHIIYRCRITLESFSQLKPLIMDIDSIDDVIVL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHHHHHCCHHHEECCCCCCEEEE
NGSHSFEMIKDVGSVLEIADRYDTWSKKGTHAIGHTRFSTESIVDRYHAHPFQSYIIPDI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCE
TVVHNGQITNYWKIREPLERKGHIFETNNDTECIVHYVADKLASGYSLEEALEQSVKDMD
EEEECCCCCEEEECCCHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
GPFSYIVGTPQGVGIAKDQLGLRPGVMAENDEVFAVASEEVSLREVMDTSEVEQISPGEV
CCEEEEEECCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHCHHHHHCCCCCCE
RVYEI
EEEEC
>Mature Secondary Structure
MCGIAGVVYKDGKLHSVGADMTRMLHALQHRGPDSAGFAIYGGLGLEENDYLLNIEVKDK
CCCCCEEEEECCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCC
PGLLDMVKETVELVSPIGSDEVIPSVENHIIYRCRITLESFSQLKPLIMDIDSIDDVIVL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHHHHHCCHHHEECCCCCCEEEE
NGSHSFEMIKDVGSVLEIADRYDTWSKKGTHAIGHTRFSTESIVDRYHAHPFQSYIIPDI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCE
TVVHNGQITNYWKIREPLERKGHIFETNNDTECIVHYVADKLASGYSLEEALEQSVKDMD
EEEECCCCCEEEECCCHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
GPFSYIVGTPQGVGIAKDQLGLRPGVMAENDEVFAVASEEVSLREVMDTSEVEQISPGEV
CCEEEEEECCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHCHHHHHCCCCCCE
RVYEI
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5-phospho-beta-D-ribosylamine; diphosphate; L-glutamate

Specific reaction: 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463