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The map label for this gene is eno

Identifier: 15678072

GI number: 15678072

Start: 21689

End: 22939

Strand: Direct

Name: eno

Synonym: MTH43

Alternate gene names: 15678072

Gene position: 21689-22939 (Clockwise)

Preceding gene: 15678071

Following gene: 15678073

Centisome position: 1.24

GC content: 52.6

Gene sequence:

>1251_bases
ATGGAAAGTATTATTGAAGATGTTAGAGTAAGGAAAATTCTTGACAGCAGGGGAAACCCGACGGTTGAGGTTGATGTTAT
AACCTGGAACGGCTTTGGAAGGGCTGCAGCCCCCAGCGGTGCAAGCACAGGTTCCAGGGAGGTTGCAGCCTTCCCATCAG
GGGGGGTTGATGAGATAATAACAGAGGTCGAGGATATAATCTCCTCAGAACTCATAGGAATGGATGCAGTGGACCTCCAG
GACATAGACCTCGTCCTAAAGGAGATCGACGGGACCGAAAATCTCTCATCTCTGGGAGGTAACACCGTGGTGGCTGTTTC
AATGGCAACCGCAAAGGCAGCAGCATCATCCTACAACATGCCCCTCTACAGGTTCCTCGGCGGAAACCTTGCAACATCCA
TACCCTACCCCCTGGGTAACATGATAAATGGGGGGGCCCACGCAGGAAAAAACGCCCCTGACATACAGGAGTTCCTGGTT
GTGCCTGTGGGTGCTGAGGACATAACCGAGGCGGTTTTCGCAAATGCAGCCGTCCATAAGAGGATAAGGGAGCTCATCCA
GAAGAAGGACCCGTCCTTTGCAGGAGGTAAGGGTGATGAGGGCGGATGGGTGCCCAGCCTCTCAAACGGGGACGCCCTGG
AGATACAGGCCACTGCCTGTGAGGAGGTAACAGATGAACTCGGTGTTGAGGTGAGACCATCGCTGGACCTTGCAGCGAGC
GAATTCTGGGACCCTGAGATTGAGAAGTACGTCTACAGGCAGGAGAACGTCCAGAAGGACACAGGCGAACAGATAGAATT
TGTTAAGGAGATAATTGAAACCTATGACATGTACTATGTCGAGGACCCCCTCCATGAGGGAGACCTTGAGGGTTTCGCCG
AGCTGACATCCCTGGTTGGTGACCGCTGCATGATCTGTGGTGACGATATCTTCGTGACCAACAGGGAGATACTCAGGGAG
GGTATAGAGATGGGTGCAGCCAACGCCATAATCATCAAGCCCAACCAGATAGGCACCCTCACAGACACCTACCTCACCGT
CAAACTGGCCCTCGAGAACAGGTACACCCCTGTGGTTTCACACAGGTCAGGTGAGACAACAGATGATACCATAGCCCACC
TTGCGGTGGCCTTCGGAGCACCCCTCATAAAGACAGGTGCAATAGGCGGTGAGAGGATAGCCAAACTCAACGAACTCATA
CGCATACAGGAAGAGATTCCATATTCAAGGATGGCTGATCTGCCATTTTAA

Upstream 100 bases:

>100_bases
GGAACTTGAAAAGAAGGTCATGCCACTGGACGTTAGAAGAGACAAATAAACTCTAATAAACCACTATTTTTCAGTCTAGA
GTTTATTATGAGGTGTTTTA

Downstream 100 bases:

>100_bases
ATATTGAAACTTATTTGAGGTGATAGATTGTCAGAACTACTAATACCACTGGACAAATACCTTGCAGCAGGACTGCACAT
TGGAACACAGCAGAAAACAG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MESIIEDVRVRKILDSRGNPTVEVDVITWNGFGRAAAPSGASTGSREVAAFPSGGVDEIITEVEDIISSELIGMDAVDLQ
DIDLVLKEIDGTENLSSLGGNTVVAVSMATAKAAASSYNMPLYRFLGGNLATSIPYPLGNMINGGAHAGKNAPDIQEFLV
VPVGAEDITEAVFANAAVHKRIRELIQKKDPSFAGGKGDEGGWVPSLSNGDALEIQATACEEVTDELGVEVRPSLDLAAS
EFWDPEIEKYVYRQENVQKDTGEQIEFVKEIIETYDMYYVEDPLHEGDLEGFAELTSLVGDRCMICGDDIFVTNREILRE
GIEMGAANAIIIKPNQIGTLTDTYLTVKLALENRYTPVVSHRSGETTDDTIAHLAVAFGAPLIKTGAIGGERIAKLNELI
RIQEEIPYSRMADLPF

Sequences:

>Translated_416_residues
MESIIEDVRVRKILDSRGNPTVEVDVITWNGFGRAAAPSGASTGSREVAAFPSGGVDEIITEVEDIISSELIGMDAVDLQ
DIDLVLKEIDGTENLSSLGGNTVVAVSMATAKAAASSYNMPLYRFLGGNLATSIPYPLGNMINGGAHAGKNAPDIQEFLV
VPVGAEDITEAVFANAAVHKRIRELIQKKDPSFAGGKGDEGGWVPSLSNGDALEIQATACEEVTDELGVEVRPSLDLAAS
EFWDPEIEKYVYRQENVQKDTGEQIEFVKEIIETYDMYYVEDPLHEGDLEGFAELTSLVGDRCMICGDDIFVTNREILRE
GIEMGAANAIIIKPNQIGTLTDTYLTVKLALENRYTPVVSHRSGETTDDTIAHLAVAFGAPLIKTGAIGGERIAKLNELI
RIQEEIPYSRMADLPF
>Mature_416_residues
MESIIEDVRVRKILDSRGNPTVEVDVITWNGFGRAAAPSGASTGSREVAAFPSGGVDEIITEVEDIISSELIGMDAVDLQ
DIDLVLKEIDGTENLSSLGGNTVVAVSMATAKAAASSYNMPLYRFLGGNLATSIPYPLGNMINGGAHAGKNAPDIQEFLV
VPVGAEDITEAVFANAAVHKRIRELIQKKDPSFAGGKGDEGGWVPSLSNGDALEIQATACEEVTDELGVEVRPSLDLAAS
EFWDPEIEKYVYRQENVQKDTGEQIEFVKEIIETYDMYYVEDPLHEGDLEGFAELTSLVGDRCMICGDDIFVTNREILRE
GIEMGAANAIIIKPNQIGTLTDTYLTVKLALENRYTPVVSHRSGETTDDTIAHLAVAFGAPLIKTGAIGGERIAKLNELI
RIQEEIPYSRMADLPF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the archaeal cel

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=422, Percent_Identity=39.0995260663507, Blast_Score=254, Evalue=9e-68,
Organism=Homo sapiens, GI301897477, Length=421, Percent_Identity=38.9548693586698, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI301897469, Length=421, Percent_Identity=38.9548693586698, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI4503571, Length=424, Percent_Identity=36.7924528301887, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI301897479, Length=411, Percent_Identity=37.2262773722628, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.1904761904762, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.1904761904762, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.1904761904762, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789141, Length=419, Percent_Identity=45.5847255369928, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI71995829, Length=423, Percent_Identity=36.6430260047281, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17536383, Length=423, Percent_Identity=36.6430260047281, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI32563855, Length=179, Percent_Identity=35.7541899441341, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321693, Length=424, Percent_Identity=35.8490566037736, Blast_Score=223, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6323985, Length=424, Percent_Identity=35.377358490566, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324974, Length=424, Percent_Identity=35.1415094339623, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6324969, Length=424, Percent_Identity=35.1415094339623, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6321968, Length=426, Percent_Identity=35.6807511737089, Blast_Score=209, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24580918, Length=424, Percent_Identity=37.7358490566038, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24580916, Length=424, Percent_Identity=37.7358490566038, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24580920, Length=424, Percent_Identity=37.7358490566038, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24580914, Length=424, Percent_Identity=37.7358490566038, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI281360527, Length=421, Percent_Identity=37.7672209026128, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI17137654, Length=421, Percent_Identity=37.7672209026128, Blast_Score=236, Evalue=3e-62,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_METTH (O26149)

Other databases:

- EMBL:   AE000666
- PIR:   A69156
- RefSeq:   NP_275186.1
- ProteinModelPortal:   O26149
- SMR:   O26149
- STRING:   O26149
- GeneID:   1470005
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH43
- NMPDR:   fig|187420.1.peg.41
- eggNOG:   arNOG04113
- HOGENOM:   HBG726599
- OMA:   LICGDDL
- ProtClustDB:   PRK00077
- BioCyc:   MetaCyc:MONOMER-14533
- BioCyc:   MTHE187420:MTH43-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44941; Mature: 44941

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 200-200 ACT_SITE 333-333 BINDING 147-147 BINDING 157-157 BINDING 281-281 BINDING 308-308 BINDING 333-333 BINDING 384-384

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIIEDVRVRKILDSRGNPTVEVDVITWNGFGRAAAPSGASTGSREVAAFPSGGVDEII
CHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCHHHHH
TEVEDIISSELIGMDAVDLQDIDLVLKEIDGTENLSSLGGNTVVAVSMATAKAAASSYNM
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEHHHHHHHHCCCC
PLYRFLGGNLATSIPYPLGNMINGGAHAGKNAPDIQEFLVVPVGAEDITEAVFANAAVHK
CHHHHHCCCHHCCCCCCCHHHCCCCCCCCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHH
RIRELIQKKDPSFAGGKGDEGGWVPSLSNGDALEIQATACEEVTDELGVEVRPSLDLAAS
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCEECCCCCHHHH
EFWDPEIEKYVYRQENVQKDTGEQIEFVKEIIETYDMYYVEDPLHEGDLEGFAELTSLVG
HCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHC
DRCMICGDDIFVTNREILREGIEMGAANAIIIKPNQIGTLTDTYLTVKLALENRYTPVVS
CCEEEECCCEEECCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEEEECCCCCCEEE
HRSGETTDDTIAHLAVAFGAPLIKTGAIGGERIAKLNELIRIQEEIPYSRMADLPF
CCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCC
>Mature Secondary Structure
MESIIEDVRVRKILDSRGNPTVEVDVITWNGFGRAAAPSGASTGSREVAAFPSGGVDEII
CHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCHHHHH
TEVEDIISSELIGMDAVDLQDIDLVLKEIDGTENLSSLGGNTVVAVSMATAKAAASSYNM
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEHHHHHHHHCCCC
PLYRFLGGNLATSIPYPLGNMINGGAHAGKNAPDIQEFLVVPVGAEDITEAVFANAAVHK
CHHHHHCCCHHCCCCCCCHHHCCCCCCCCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHH
RIRELIQKKDPSFAGGKGDEGGWVPSLSNGDALEIQATACEEVTDELGVEVRPSLDLAAS
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCEECCCCCHHHH
EFWDPEIEKYVYRQENVQKDTGEQIEFVKEIIETYDMYYVEDPLHEGDLEGFAELTSLVG
HCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHC
DRCMICGDDIFVTNREILREGIEMGAANAIIIKPNQIGTLTDTYLTVKLALENRYTPVVS
CCEEEECCCEEECCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEEEECCCCCCEEE
HRSGETTDDTIAHLAVAFGAPLIKTGAIGGERIAKLNELIRIQEEIPYSRMADLPF
CCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463