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The map label for this gene is pbp2A

Identifier: 15674090

GI number: 15674090

Start: 2177041

End: 2179272

Strand: Reverse

Name: pbp2A

Synonym: L160485

Alternate gene names: 15674090

Gene position: 2179272-2177041 (Counterclockwise)

Preceding gene: 15674091

Following gene: 15674089

Centisome position: 92.12

GC content: 37.37

Gene sequence:

>2232_bases
ATGTCTGAAAATAATAATTTCAGTCGGCGTAATAAAAAGGAATCTAAAAAAAATAGTTTAAAAATTCCTAATTTACGCCC
TAAAAAACAAAAAAAGCTTGAAGAAGAAATAGAAAAACCGGCTAAAACGAAGTTCGGTAAATTCATGAAACCAATTAAAC
GGTTCTGGAAACGTTATAATCTGACAAAAATCACAATTATTTTTGTCTTAGTAGCCATTGTTTCGACAGGTTCATATCTT
TTTTACTTAGCTAAAACAGCTAATGTTAAAGTACTGCAAAGTTCTATTTCGGCACAAACAGTCATTTATGACAAAGATAA
TAATGAAGCTGGAAATCTTTATGGTCAAAAAGGAACACCGGTCAAGATTGACCAAATTTCCAAAAATATTACAAATGCTG
TTGTGGCAACTGAAGATAGAACTTTCTATAAAAATCATGGTGTCAATTTGAAACGTTTTGCTCTAGCAGCCGTTACTCTT
GGGCGTTTTGGTGGTGGTTCAACAATCACTCAGCAATTGGCCAAAAATGCTTATTTGACTCAAGAACAAACGATTGACCG
TAAAGCGCGTGAGTTTTTCTTGGCACTTGAAATCAATAAACATTATAGTAAAGATGAGATTCTTGATATGTATCTGAATA
ATTCTTACTTTGGTAATGGTGTTTGGGGAATTCAGGATGCAGCTTTGAAATACTTTGGTGTTCCAGCAAGTGAAGTAACT
GTTGATGAAGCAGCCAGTCTTGCGGGAATGTTAAAAGGGCCAGAAATTTATAATCCTCTTTACGAAAAAGGAAAATATGC
GACTGACCGACGTAATACAGTTTTGCAAAATATGGTTAATGCTGGTTATTTGGAGCAGTCACAGGCAGATACTTTTATGA
AAGTTGACTTACAAGCTCAATTGCAAGACAATTATCAATCTAAAAGTAGTCAATATAAATATCCAAGCTATTATAATGCT
GTAATTTCTGAAGCGGAACGCAAGTATGGTCTGACATTACAAGAAATCATGAATAATGGTTACAAGATATATACGGGCAT
GGACCAAAATATGCAATCCGGTTTGCAAAAGACTTATTCTGACCCAAGTTTCTTCCCACAAGCATCTGATGGAACTTATG
CCCAATCTGCTTCGGTTGCTATTGACCCTAAAACGGGAGCTGTCAATGCTCTTGTCGGAAATGTTAATACAGAAGGTTCA
AATAGCTTTACTGATTATAACTATGCCACAATGTCTAAACGTTCTCCTGGTTCAGTTATCAAACCTCTTATTGTTTACGC
TCCAGCAATTGAAGCGGGGTGGTCAATTGATAAAACGGTTGATGATTCACCAGCAGATTATAATGGCTGGAAACCAACCG
ATTTTGATAATCAATGGCGTGGACAAATCCCAATGTATACTGCTTTAGCAAATTCATATAATATTCCAGCTATTAATACA
TATCAAGCGATTGGACCAAAAGTCGGAAATGCTTTAGGGCGTGAGTTTGGTTTAGATTTAAGTAGTAAAAATGATGTTTT
ACCGACGGCACTCGGAGCTGGAGTTGAAACGAATCCATGGCAAATTGCTCAGGCTTATCAAGTTTTCGCAAATGGCGGAG
TGATGAATGATGCGCATTTGATTACTAAAATTGAAAATGCTGCAGGACAAGTTGTAAAAACAGCAAAGGTAACCAAAAAA
CGGGTTATTAGTAAAGATACAGCTGACAAAATGACTCAAATGATGCTGGGGACTTATACTAATGGTTCTGCTTGGAAAGC
TTCACCTAAGTCTTATACACTTGCTGGTAAGACAGGAACCAATGAAGACCAAGACCAATGGGTAGTAGGTTATACACCGG
ATGTGGTTATGGCTCTCTGGGTTGGTTATGCGGATGGGAAATATAAACTGACAGGTTCATCAGAAGGACAAACCTCAGTC
ATTTTCCGTCAAGAAGCTAGTTATATGTTGCCTTATACTAAAGGAACGGCATTTACAGTTGAAAATCCTTATGCGGAAGC
AGGTGTTGCCGCACAAGAACCTTATTGGACTCAACAAAGACAATATCAAGATGATATTGTGGACCAAGAACAAGCAGAGG
CTCACACGACTGGAAATACACCAGAAAGCTCTAGTTCTAGCTCTTCATCGTCTTCAGATTCTGGTGGTTTGGACCTCGGA
AAAGTGGGTAAAGATATTGGAGATGCAGCTAAAAATGCTTGGGATAAAGTTAAAGGAATTTTTGGCAACTAA

Upstream 100 bases:

>100_bases
ACAAAGATGTAAGGCAAAAAAAGCAGAAAAGTATTAAAATAAGGTATTGCTGTATTCATTTGAGTAAGTAAAGAAATAAA
AAGCATCTGAGGAACGATAT

Downstream 100 bases:

>100_bases
AACTGAAAAAAGAGGTAGAAATATGGTAGAATTAGAGGTAAAGCGTAGTAGTTTTAAGACAAGAAAATTTTAAAGAATAA
AGATAGAAATCGAGAAGAAA

Product: penicillin-binding protein 2a

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MSENNNFSRRNKKESKKNSLKIPNLRPKKQKKLEEEIEKPAKTKFGKFMKPIKRFWKRYNLTKITIIFVLVAIVSTGSYL
FYLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTPVKIDQISKNITNAVVATEDRTFYKNHGVNLKRFALAAVTL
GRFGGGSTITQQLAKNAYLTQEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVT
VDEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADTFMKVDLQAQLQDNYQSKSSQYKYPSYYNA
VISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYSDPSFFPQASDGTYAQSASVAIDPKTGAVNALVGNVNTEGS
NSFTDYNYATMSKRSPGSVIKPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINT
YQAIGPKVGNALGREFGLDLSSKNDVLPTALGAGVETNPWQIAQAYQVFANGGVMNDAHLITKIENAAGQVVKTAKVTKK
RVISKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGTNEDQDQWVVGYTPDVVMALWVGYADGKYKLTGSSEGQTSV
IFRQEASYMLPYTKGTAFTVENPYAEAGVAAQEPYWTQQRQYQDDIVDQEQAEAHTTGNTPESSSSSSSSSSDSGGLDLG
KVGKDIGDAAKNAWDKVKGIFGN

Sequences:

>Translated_743_residues
MSENNNFSRRNKKESKKNSLKIPNLRPKKQKKLEEEIEKPAKTKFGKFMKPIKRFWKRYNLTKITIIFVLVAIVSTGSYL
FYLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTPVKIDQISKNITNAVVATEDRTFYKNHGVNLKRFALAAVTL
GRFGGGSTITQQLAKNAYLTQEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVT
VDEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADTFMKVDLQAQLQDNYQSKSSQYKYPSYYNA
VISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYSDPSFFPQASDGTYAQSASVAIDPKTGAVNALVGNVNTEGS
NSFTDYNYATMSKRSPGSVIKPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINT
YQAIGPKVGNALGREFGLDLSSKNDVLPTALGAGVETNPWQIAQAYQVFANGGVMNDAHLITKIENAAGQVVKTAKVTKK
RVISKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGTNEDQDQWVVGYTPDVVMALWVGYADGKYKLTGSSEGQTSV
IFRQEASYMLPYTKGTAFTVENPYAEAGVAAQEPYWTQQRQYQDDIVDQEQAEAHTTGNTPESSSSSSSSSSDSGGLDLG
KVGKDIGDAAKNAWDKVKGIFGN
>Mature_742_residues
SENNNFSRRNKKESKKNSLKIPNLRPKKQKKLEEEIEKPAKTKFGKFMKPIKRFWKRYNLTKITIIFVLVAIVSTGSYLF
YLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTPVKIDQISKNITNAVVATEDRTFYKNHGVNLKRFALAAVTLG
RFGGGSTITQQLAKNAYLTQEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVTV
DEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADTFMKVDLQAQLQDNYQSKSSQYKYPSYYNAV
ISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYSDPSFFPQASDGTYAQSASVAIDPKTGAVNALVGNVNTEGSN
SFTDYNYATMSKRSPGSVIKPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINTY
QAIGPKVGNALGREFGLDLSSKNDVLPTALGAGVETNPWQIAQAYQVFANGGVMNDAHLITKIENAAGQVVKTAKVTKKR
VISKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGTNEDQDQWVVGYTPDVVMALWVGYADGKYKLTGSSEGQTSVI
FRQEASYMLPYTKGTAFTVENPYAEAGVAAQEPYWTQQRQYQDDIVDQEQAEAHTTGNTPESSSSSSSSSSDSGGLDLGK
VGKDIGDAAKNAWDKVKGIFGN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=530, Percent_Identity=31.1320754716981, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI87082258, Length=303, Percent_Identity=35.6435643564356, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1788867, Length=574, Percent_Identity=27.0034843205575, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1789601, Length=177, Percent_Identity=37.8531073446328, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 81976; Mature: 81844

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENNNFSRRNKKESKKNSLKIPNLRPKKQKKLEEEIEKPAKTKFGKFMKPIKRFWKRYN
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTKITIIFVLVAIVSTGSYLFYLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTP
CHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCC
VKIDQISKNITNAVVATEDRTFYKNHGVNLKRFALAAVTLGRFGGGSTITQQLAKNAYLT
EEHHHHHHHHHHCEEECCCCEEHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
QEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVT
HHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
VDEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADTFMKVDLQAQ
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHH
LQDNYQSKSSQYKYPSYYNAVISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYS
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCHHHHHHHHHHCC
DPSFFPQASDGTYAQSASVAIDPKTGAVNALVGNVNTEGSNSFTDYNYATMSKRSPGSVI
CCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHH
KPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINT
CCHHEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCCC
YQAIGPKVGNALGREFGLDLSSKNDVLPTALGAGVETNPWQIAQAYQVFANGGVMNDAHL
HHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
ITKIENAAGQVVKTAKVTKKRVISKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
NEDQDQWVVGYTPDVVMALWVGYADGKYKLTGSSEGQTSVIFRQEASYMLPYTKGTAFTV
CCCCCCEEEECCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCEECEECCCCEEEE
ENPYAEAGVAAQEPYWTQQRQYQDDIVDQEQAEAHTTGNTPESSSSSSSSSSDSGGLDLG
CCCCHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
KVGKDIGDAAKNAWDKVKGIFGN
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SENNNFSRRNKKESKKNSLKIPNLRPKKQKKLEEEIEKPAKTKFGKFMKPIKRFWKRYN
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTKITIIFVLVAIVSTGSYLFYLAKTANVKVLQSSISAQTVIYDKDNNEAGNLYGQKGTP
CHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCC
VKIDQISKNITNAVVATEDRTFYKNHGVNLKRFALAAVTLGRFGGGSTITQQLAKNAYLT
EEHHHHHHHHHHCEEECCCCEEHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
QEQTIDRKAREFFLALEINKHYSKDEILDMYLNNSYFGNGVWGIQDAALKYFGVPASEVT
HHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
VDEAASLAGMLKGPEIYNPLYEKGKYATDRRNTVLQNMVNAGYLEQSQADTFMKVDLQAQ
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHH
LQDNYQSKSSQYKYPSYYNAVISEAERKYGLTLQEIMNNGYKIYTGMDQNMQSGLQKTYS
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCHHHHHHHHHHCC
DPSFFPQASDGTYAQSASVAIDPKTGAVNALVGNVNTEGSNSFTDYNYATMSKRSPGSVI
CCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHH
KPLIVYAPAIEAGWSIDKTVDDSPADYNGWKPTDFDNQWRGQIPMYTALANSYNIPAINT
CCHHEEECCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCCC
YQAIGPKVGNALGREFGLDLSSKNDVLPTALGAGVETNPWQIAQAYQVFANGGVMNDAHL
HHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
ITKIENAAGQVVKTAKVTKKRVISKDTADKMTQMMLGTYTNGSAWKASPKSYTLAGKTGT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
NEDQDQWVVGYTPDVVMALWVGYADGKYKLTGSSEGQTSVIFRQEASYMLPYTKGTAFTV
CCCCCCEEEECCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCEECEECCCCEEEE
ENPYAEAGVAAQEPYWTQQRQYQDDIVDQEQAEAHTTGNTPESSSSSSSSSSDSGGLDLG
CCCCHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
KVGKDIGDAAKNAWDKVKGIFGN
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]