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The map label for this gene is pheS

Identifier: 15673911

GI number: 15673911

Start: 2009087

End: 2010127

Strand: Reverse

Name: pheS

Synonym: L0354

Alternate gene names: 15673911

Gene position: 2010127-2009087 (Counterclockwise)

Preceding gene: 15673914

Following gene: 15673910

Centisome position: 84.97

GC content: 39.1

Gene sequence:

>1041_bases
ATGAACTTACAAGAAAAAATTGAAGACCTTCGCAAGCGGACTTTGTCTGACTTACTCTCTGTTGCTGATGAAAAAACACT
GAATAATTTAAGAACTGTGATGCTTGGTAAAAAGGGTGAATTGACTGAAATTCTGAAAGGGATGAAAGACCTAACTAATG
AAGAACGTCCAGTTATTGGCGCACTTGCCAATGCTTTTCGTGATGAGTTTGGGGCAAAATTTGAAGCTAAAAAAGTGGAA
ATTGAACAAGCAGTAATGAATGCCGCTTTAGAATCAGAAACTTTGGATGTGACTTTGCCAGGAAAAGCTCAGAAAAAAGG
AAGTCGCCATATCTTAACTCAAACGCAAGAAGAAATTGAAGAAATTTTCCTTGGAATGGGTTATGAAATTGTTGATGGCT
ATGAAGTGGAAACGGACCATTATAATTTTGAGCGAATGAATCTTCCAAAAGATCATCCAGCTCGTGATATGCAAGATACC
TTCTATATCACTAATGAAGTTTTACTTCGTACGCATACTTCACCTATGCAAGCTCGGACAATGGATGCTCATGATTTCTC
TAAAGGTGGTTTACGCATGATTGCTCCTGGTCGTGTTTATCGTCGTGATACAGATGATGCCACTCACAGCCACCAATTTC
ATCAAATTGAAGGTTTGGTTGTAGATAAAAATATCACAATGGCGGACCTTAAAGGAACGCTTGACCTTGTCATGAAAAAA
ATGTTTGGTCAAGACCGTGAATTACGCTGGCGTCCAAGTTATTTCCCATTTACTGAACCTTCTGTTGAGGTTGATATTTC
TTGTTTCAAATGTGGCGGAAAAGGCTGTAACGTCTGCAAACATACCGGCTGGATTGAAATTCTTGGTGCAGGTATGGTCC
ATCCCAATGTGCTTGAAATGTCTGGTTTAGATTCTTCAGTTTATTCTGGTTTTGCTTTCGGGCTTGGTCAAGAACGTATT
GCGATGCTCCGTTATGGAATCAATGATATTCGTGGATTCTATCAAGGAGATGTACGATTCTTAGAGCAATTTGGAAAATA
A

Upstream 100 bases:

>100_bases
TTGGAGATGAGAGTTGTGGTATTTTTTATTTTCTATTAAATCTGGCTCAAATCTCTTTTTAATAACAAATATATATTTTT
CATAAGTTGAAAGGATAAAA

Downstream 100 bases:

>100_bases
AAAGGTCAAAAGACTGTGAATAGAAAGTGAAATAAAATGCAAGTATCATATAAATGGTTAAAAGAGTTGGTGCCTAATCT
TAGAGCGACATCGGCTGAAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIGALANAFRDEFGAKFEAKKVE
IEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIEEIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDT
FYITNEVLLRTHTSPMQARTMDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
MFGQDRELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEMSGLDSSVYSGFAFGLGQERI
AMLRYGINDIRGFYQGDVRFLEQFGK

Sequences:

>Translated_346_residues
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIGALANAFRDEFGAKFEAKKVE
IEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIEEIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDT
FYITNEVLLRTHTSPMQARTMDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
MFGQDRELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEMSGLDSSVYSGFAFGLGQERI
AMLRYGINDIRGFYQGDVRFLEQFGK
>Mature_346_residues
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIGALANAFRDEFGAKFEAKKVE
IEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIEEIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDT
FYITNEVLLRTHTSPMQARTMDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
MFGQDRELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEMSGLDSSVYSGFAFGLGQERI
AMLRYGINDIRGFYQGDVRFLEQFGK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=293, Percent_Identity=31.3993174061433, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4758340, Length=270, Percent_Identity=31.4814814814815, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1788007, Length=324, Percent_Identity=47.2222222222222, Blast_Score=314, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI32563657, Length=277, Percent_Identity=31.0469314079422, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=277, Percent_Identity=31.0469314079422, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=252, Percent_Identity=35.3174603174603, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321087, Length=366, Percent_Identity=28.9617486338798, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=298, Percent_Identity=28.5234899328859, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI18858079, Length=277, Percent_Identity=31.4079422382672, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI17137424, Length=295, Percent_Identity=30.1694915254237, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_LACLA (Q9CEB4)

Other databases:

- EMBL:   AE005176
- PIR:   A86866
- RefSeq:   NP_268086.1
- ProteinModelPortal:   Q9CEB4
- SMR:   Q9CEB4
- GeneID:   1115597
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0354
- NMPDR:   fig|272623.1.peg.1976
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   LLAC272623:L0354-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39273; Mature: 39273

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHH
ALANAFRDEFGAKFEAKKVEIEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCHHHHHHHHHH
EIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDTFYITNEVLLRTHTSPMQART
HHHHHCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHEEEECCEEEEECCCCCHHHC
MDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
CCCCCCCCCCEEEECCCCEEECCCCCCHHCCHHHEECCEEEECCCCHHHHHHHHHHHHHH
MFGQDRELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEM
HHCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCHHEEEECCCCCCCEEEE
SGLDSSVYSGFAFGLGQERIAMLRYGINDIRGFYQGDVRFLEQFGK
CCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHH
ALANAFRDEFGAKFEAKKVEIEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCHHHHHHHHHH
EIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDTFYITNEVLLRTHTSPMQART
HHHHHCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHEEEECCEEEEECCCCCHHHC
MDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
CCCCCCCCCCEEEECCCCEEECCCCCCHHCCHHHEECCEEEECCCCHHHHHHHHHHHHHH
MFGQDRELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEM
HHCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCHHEEEECCCCCCCEEEE
SGLDSSVYSGFAFGLGQERIAMLRYGINDIRGFYQGDVRFLEQFGK
CCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471