Click here to switch to the map view.

The map label for this gene is pheT

Identifier: 15673910

GI number: 15673910

Start: 2006657

End: 2009050

Strand: Reverse

Name: pheT

Synonym: L0355

Alternate gene names: 15673910

Gene position: 2009050-2006657 (Counterclockwise)

Preceding gene: 15673911

Following gene: 30024062

Centisome position: 84.93

GC content: 37.59

Gene sequence:

>2394_bases
ATGCAAGTATCATATAAATGGTTAAAAGAGTTGGTGCCTAATCTTAGAGCGACATCGGCTGAATTAGAACAAAAGATGTC
AACGTCTGGGATTGAGGTTGAAGGCGTGACAAGTCCAATGGAAGGTTTGTCAAAACTTGTTGTGGGAGAAATTTTATCAA
GTGAAGATATTCCTGACACACACTTACACATCACACAAGTGAATGTTGGAGCTGAAGAATCATTGCAAATTGTTTGTGGT
GCGCCAAATGTTCGTGTGGGTATGAAAGTAATTGTAGCACTTGTGGGTGCTCGAATTGCTGATAATTATAAAATCAAAAA
AGGTAAAATTCGAGGAGTTGAATCTCTCGGGATGCTCTGTGCGCTTGATGAAATTGGGATTGATGAAAAAATTAATCCAA
TGAAGCATGAGGATGGTATTTTTGAAATGCCAGCGGATGCTAAAGTTGGTGATAGTATTTTCTCTTATCTTGATATGGAT
GATGAAATCATTGAATTATCAATTACACCAAACCGTGCGGATGCTTTGTCAATGCATGGGGCAGCTTGGGAAGTTGGTGC
TATTTATGGTTTACCAGTTCAATTAGAAAAGAAAGATTTAGTAGAAGCTTCTGAATCAGCAGCAAGTAAAATTGCAGTAA
AAGTTGAAACAGATAAGGTTCCAACATATAAAATTCGTTTGATTGAAGGGGTTAAGATTGCTAAATCACCACAATGGTTG
CAAAACCGTTTGATGAATGCTGGTGTGAAACCAATCAACAATGTAGTGGATGTGACGAATTATGTTTTGATGGCTTTTGG
TCAACCTTTGCATAGTTTTGATTTTTCTAAATTTGGTTCTGATGAAATTTTGGTTCGTCAAGCTAAGGCTGGTGAAAAAA
TGACAACCTTAGACCATGTTGAACGTGAATTAGATGATTCTGATATTGTGGTTACCGCAAATGGACTTCCTGTAGCGCTT
GGTGGTGTTATGGGTGGCGCTGATAGTGAAATTACTGACGAAACAACAAGTGTTGCCCTTGAAGCGGCTCTATTTGATGG
GACTTCAATTCGTAAAACTTCTCAAAAATTTGCTTTGCGTTCGGAAGCATCAAGTCGTTTTGAAAAAGGAATTAATGAAG
GAACGGTTCGTGAGGCACTTGATTTTGCAGCAGCAATGATAGTTGAATTGGCTGGTGGAAAAGTTCTTTCAGGTGTTGTT
GAATCAAATGATTATCAACCTATTTTGCCAAAAGTATCAATTACTTTGAGTCGTGTTAATAGTGCTTTGGGAACAGATTT
ATCTCTTGAGACTGTGGAAAAAATTTTTGTTCAACTTGGCTTTGGGGTTGAAGTTGAGGGTGAAAAATTTACTTGTGAAA
TCCCATCACGTCGTTGGGATATTCATATTGAAGCTGATTTAGTTGAAGAAGTAGCTCGTATTTATGGCTATGATAATTTG
CCAAGTACTTTGCCTTCCTCACAAAATGCTGGTGAATTGACTCAAATGCAAAAATTCCGTCGTACAGTGCGAACAGGTCT
CGAAAGTTCAGGGCTTAATGAAGTTATTGGTTATTCGCTTGTAACTCCAGAAAAAGCAACTGAATTTGTGGGTCAGCTTG
AAACAACAACGCTTATGATGCCAATGACCGAAGATCGTCAAACACTTCGTGCAAACATGATTCCAGGACTTTTGGATATT
GTAAATTACAATCAGAATCGTAAAAATGCTGATGTGGCAATTTATGAAATTGGCAATATTTTCTTACCAAATCCAGATGA
TATTCGTCCGATAGAAGTACCAAACCTTGCTTTTGCTATTTCTGGAAATGTTGTGGATAAATCATATAATGGGCAAGCTG
TTCCTGTTGATTTCTACTATGCTAAGGGAATTGTGGAAAACCTTCTCGAAGCATACAAAGAAGTAGAATTTATTCCAAGC
AATAATCAAGCAGCGATGCACCCAGGACGGACTGCTGTCATTAAAATTAATGGTCGTGTGGCTGGTTTTGTAGGACAAAT
TCATCCGGCGACAGCTAAAAAATACGATATTGCAGAAACTTATGTTGCTGGTCTTGATATGCAAGTGATGCTTGAAGAAT
TACCAGCTCAAACTATCTTTACTGACATTCCGAAAGTTCAAGCTGTTCACCGTGATATCGCACTTTTGATTGATGCTGAA
GTGACACATGCACAAATTGTTTCTGTCATTAAATCAAGTCGTATCAAAACACTTAGTCAAGTTGAGTTATTTGATATTTA
TCAAGGCAAAAACTTACCGGCTGGTAAGAAATCAATGGCTTACAGTCTCACTTTCCAACCTGTTGAAAATACAATGACAG
ATGAAGAAATTACGGCTGCGGTCAATAAAATTACAAAAAATCTTGTGGAAAAATTGGATATAGAAATCCGTTAA

Upstream 100 bases:

>100_bases
AATCAATGATATTCGTGGATTCTATCAAGGAGATGTACGATTCTTAGAGCAATTTGGAAAATAAAAAGGTCAAAAGACTG
TGAATAGAAAGTGAAATAAA

Downstream 100 bases:

>100_bases
TTTAAAAAAGTACTGACAAAAAAACTGTCAGTAAAAATCTCTATTGGTATTTTACTGATAGAGATTTTTTTGCAAATAAA
TAATAAAGAAGCGAATGAAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 797; Mature: 797

Protein sequence:

>797_residues
MQVSYKWLKELVPNLRATSAELEQKMSTSGIEVEGVTSPMEGLSKLVVGEILSSEDIPDTHLHITQVNVGAEESLQIVCG
APNVRVGMKVIVALVGARIADNYKIKKGKIRGVESLGMLCALDEIGIDEKINPMKHEDGIFEMPADAKVGDSIFSYLDMD
DEIIELSITPNRADALSMHGAAWEVGAIYGLPVQLEKKDLVEASESAASKIAVKVETDKVPTYKIRLIEGVKIAKSPQWL
QNRLMNAGVKPINNVVDVTNYVLMAFGQPLHSFDFSKFGSDEILVRQAKAGEKMTTLDHVERELDDSDIVVTANGLPVAL
GGVMGGADSEITDETTSVALEAALFDGTSIRKTSQKFALRSEASSRFEKGINEGTVREALDFAAAMIVELAGGKVLSGVV
ESNDYQPILPKVSITLSRVNSALGTDLSLETVEKIFVQLGFGVEVEGEKFTCEIPSRRWDIHIEADLVEEVARIYGYDNL
PSTLPSSQNAGELTQMQKFRRTVRTGLESSGLNEVIGYSLVTPEKATEFVGQLETTTLMMPMTEDRQTLRANMIPGLLDI
VNYNQNRKNADVAIYEIGNIFLPNPDDIRPIEVPNLAFAISGNVVDKSYNGQAVPVDFYYAKGIVENLLEAYKEVEFIPS
NNQAAMHPGRTAVIKINGRVAGFVGQIHPATAKKYDIAETYVAGLDMQVMLEELPAQTIFTDIPKVQAVHRDIALLIDAE
VTHAQIVSVIKSSRIKTLSQVELFDIYQGKNLPAGKKSMAYSLTFQPVENTMTDEEITAAVNKITKNLVEKLDIEIR

Sequences:

>Translated_797_residues
MQVSYKWLKELVPNLRATSAELEQKMSTSGIEVEGVTSPMEGLSKLVVGEILSSEDIPDTHLHITQVNVGAEESLQIVCG
APNVRVGMKVIVALVGARIADNYKIKKGKIRGVESLGMLCALDEIGIDEKINPMKHEDGIFEMPADAKVGDSIFSYLDMD
DEIIELSITPNRADALSMHGAAWEVGAIYGLPVQLEKKDLVEASESAASKIAVKVETDKVPTYKIRLIEGVKIAKSPQWL
QNRLMNAGVKPINNVVDVTNYVLMAFGQPLHSFDFSKFGSDEILVRQAKAGEKMTTLDHVERELDDSDIVVTANGLPVAL
GGVMGGADSEITDETTSVALEAALFDGTSIRKTSQKFALRSEASSRFEKGINEGTVREALDFAAAMIVELAGGKVLSGVV
ESNDYQPILPKVSITLSRVNSALGTDLSLETVEKIFVQLGFGVEVEGEKFTCEIPSRRWDIHIEADLVEEVARIYGYDNL
PSTLPSSQNAGELTQMQKFRRTVRTGLESSGLNEVIGYSLVTPEKATEFVGQLETTTLMMPMTEDRQTLRANMIPGLLDI
VNYNQNRKNADVAIYEIGNIFLPNPDDIRPIEVPNLAFAISGNVVDKSYNGQAVPVDFYYAKGIVENLLEAYKEVEFIPS
NNQAAMHPGRTAVIKINGRVAGFVGQIHPATAKKYDIAETYVAGLDMQVMLEELPAQTIFTDIPKVQAVHRDIALLIDAE
VTHAQIVSVIKSSRIKTLSQVELFDIYQGKNLPAGKKSMAYSLTFQPVENTMTDEEITAAVNKITKNLVEKLDIEIR
>Mature_797_residues
MQVSYKWLKELVPNLRATSAELEQKMSTSGIEVEGVTSPMEGLSKLVVGEILSSEDIPDTHLHITQVNVGAEESLQIVCG
APNVRVGMKVIVALVGARIADNYKIKKGKIRGVESLGMLCALDEIGIDEKINPMKHEDGIFEMPADAKVGDSIFSYLDMD
DEIIELSITPNRADALSMHGAAWEVGAIYGLPVQLEKKDLVEASESAASKIAVKVETDKVPTYKIRLIEGVKIAKSPQWL
QNRLMNAGVKPINNVVDVTNYVLMAFGQPLHSFDFSKFGSDEILVRQAKAGEKMTTLDHVERELDDSDIVVTANGLPVAL
GGVMGGADSEITDETTSVALEAALFDGTSIRKTSQKFALRSEASSRFEKGINEGTVREALDFAAAMIVELAGGKVLSGVV
ESNDYQPILPKVSITLSRVNSALGTDLSLETVEKIFVQLGFGVEVEGEKFTCEIPSRRWDIHIEADLVEEVARIYGYDNL
PSTLPSSQNAGELTQMQKFRRTVRTGLESSGLNEVIGYSLVTPEKATEFVGQLETTTLMMPMTEDRQTLRANMIPGLLDI
VNYNQNRKNADVAIYEIGNIFLPNPDDIRPIEVPNLAFAISGNVVDKSYNGQAVPVDFYYAKGIVENLLEAYKEVEFIPS
NNQAAMHPGRTAVIKINGRVAGFVGQIHPATAKKYDIAETYVAGLDMQVMLEELPAQTIFTDIPKVQAVHRDIALLIDAE
VTHAQIVSVIKSSRIKTLSQVELFDIYQGKNLPAGKKSMAYSLTFQPVENTMTDEEITAAVNKITKNLVEKLDIEIR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=390, Percent_Identity=23.0769230769231, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1788006, Length=811, Percent_Identity=35.8816276202219, Blast_Score=446, Evalue=1e-126,
Organism=Drosophila melanogaster, GI21355211, Length=390, Percent_Identity=21.5384615384615, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_LACLA (Q9CEB5)

Other databases:

- EMBL:   AE005176
- PIR:   H86865
- RefSeq:   NP_268085.1
- ProteinModelPortal:   Q9CEB5
- SMR:   Q9CEB5
- GeneID:   1115596
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0355
- NMPDR:   fig|272623.1.peg.1975
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BioCyc:   LLAC272623:L0355-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 87198; Mature: 87198

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSYKWLKELVPNLRATSAELEQKMSTSGIEVEGVTSPMEGLSKLVVGEILSSEDIPDT
CCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCC
HLHITQVNVGAEESLQIVCGAPNVRVGMKVIVALVGARIADNYKIKKGKIRGVESLGMLC
EEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
ALDEIGIDEKINPMKHEDGIFEMPADAKVGDSIFSYLDMDDEIIELSITPNRADALSMHG
HHHHCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCC
AAWEVGAIYGLPVQLEKKDLVEASESAASKIAVKVETDKVPTYKIRLIEGVKIAKSPQWL
CCEECCEEECCCEEECHHHHHHHHHHHHCEEEEEEECCCCCEEEEEEEECCEECCCHHHH
QNRLMNAGVKPINNVVDVTNYVLMAFGQPLHSFDFSKFGSDEILVRQAKAGEKMTTLDHV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHCCCHHCCCCHHEEEEHHCCCCHHHHHHH
ERELDDSDIVVTANGLPVALGGVMGGADSEITDETTSVALEAALFDGTSIRKTSQKFALR
HHHCCCCCEEEEECCCCEEECCCCCCCCCCCCCHHHHHEEEHHHCCCCCHHHHHHHHHHH
SEASSRFEKGINEGTVREALDFAAAMIVELAGGKVLSGVVESNDYQPILPKVSITLSRVN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHEEHHHCCCCCCCCCCHHHHHHHHHH
SALGTDLSLETVEKIFVQLGFGVEVEGEKFTCEIPSRRWDIHIEADLVEEVARIYGYDNL
HHHCCCCCHHHHHHHHHHHCCCEEECCCEEEEECCCCEEEEEEEHHHHHHHHHHCCCCCC
PSTLPSSQNAGELTQMQKFRRTVRTGLESSGLNEVIGYSLVTPEKATEFVGQLETTTLMM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCEEECCHHHHHHHHHHCCEEEEE
PMTEDRQTLRANMIPGLLDIVNYNQNRKNADVAIYEIGNIFLPNPDDIRPIEVPNLAFAI
CCCCCHHHHHHHHCHHHHHHHCCCCCCCCCCEEEEEECCEECCCCCCCCCEECCCEEEEE
SGNVVDKSYNGQAVPVDFYYAKGIVENLLEAYKEVEFIPSNNQAAMHPGRTAVIKINGRV
ECCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHEEECCCCCCCEECCCCEEEEEECCEE
AGFVGQIHPATAKKYDIAETYVAGLDMQVMLEELPAQTIFTDIPKVQAVHRDIALLIDAE
EEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHCCHHHHHHHCCEEEEEECC
VTHAQIVSVIKSSRIKTLSQVELFDIYQGKNLPAGKKSMAYSLTFQPVENTMTDEEITAA
CHHHHHHHHHHHHHHHHHHHHHEEEHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHH
VNKITKNLVEKLDIEIR
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQVSYKWLKELVPNLRATSAELEQKMSTSGIEVEGVTSPMEGLSKLVVGEILSSEDIPDT
CCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCC
HLHITQVNVGAEESLQIVCGAPNVRVGMKVIVALVGARIADNYKIKKGKIRGVESLGMLC
EEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
ALDEIGIDEKINPMKHEDGIFEMPADAKVGDSIFSYLDMDDEIIELSITPNRADALSMHG
HHHHCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCC
AAWEVGAIYGLPVQLEKKDLVEASESAASKIAVKVETDKVPTYKIRLIEGVKIAKSPQWL
CCEECCEEECCCEEECHHHHHHHHHHHHCEEEEEEECCCCCEEEEEEEECCEECCCHHHH
QNRLMNAGVKPINNVVDVTNYVLMAFGQPLHSFDFSKFGSDEILVRQAKAGEKMTTLDHV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHCCCHHCCCCHHEEEEHHCCCCHHHHHHH
ERELDDSDIVVTANGLPVALGGVMGGADSEITDETTSVALEAALFDGTSIRKTSQKFALR
HHHCCCCCEEEEECCCCEEECCCCCCCCCCCCCHHHHHEEEHHHCCCCCHHHHHHHHHHH
SEASSRFEKGINEGTVREALDFAAAMIVELAGGKVLSGVVESNDYQPILPKVSITLSRVN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHEEHHHCCCCCCCCCCHHHHHHHHHH
SALGTDLSLETVEKIFVQLGFGVEVEGEKFTCEIPSRRWDIHIEADLVEEVARIYGYDNL
HHHCCCCCHHHHHHHHHHHCCCEEECCCEEEEECCCCEEEEEEEHHHHHHHHHHCCCCCC
PSTLPSSQNAGELTQMQKFRRTVRTGLESSGLNEVIGYSLVTPEKATEFVGQLETTTLMM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCEEECCHHHHHHHHHHCCEEEEE
PMTEDRQTLRANMIPGLLDIVNYNQNRKNADVAIYEIGNIFLPNPDDIRPIEVPNLAFAI
CCCCCHHHHHHHHCHHHHHHHCCCCCCCCCCEEEEEECCEECCCCCCCCCEECCCEEEEE
SGNVVDKSYNGQAVPVDFYYAKGIVENLLEAYKEVEFIPSNNQAAMHPGRTAVIKINGRV
ECCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHEEECCCCCCCEECCCCEEEEEECCEE
AGFVGQIHPATAKKYDIAETYVAGLDMQVMLEELPAQTIFTDIPKVQAVHRDIALLIDAE
EEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHCCHHHHHHHCCEEEEEECC
VTHAQIVSVIKSSRIKTLSQVELFDIYQGKNLPAGKKSMAYSLTFQPVENTMTDEEITAA
CHHHHHHHHHHHHHHHHHHHHHEEEHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHH
VNKITKNLVEKLDIEIR
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471