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The map label for this gene is murE

Identifier: 15673788

GI number: 15673788

Start: 1870593

End: 1872044

Strand: Direct

Name: murE

Synonym: L53929

Alternate gene names: 15673788

Gene position: 1870593-1872044 (Clockwise)

Preceding gene: 15673785

Following gene: 15673790

Centisome position: 79.08

GC content: 36.23

Gene sequence:

>1452_bases
ATGATTAGGCTTGAACAAGTTATAGAAATACTAAAAAAAGATAATAATTTCCGTGAGATTTCTTCTGCTGGAGAATATTA
TTTCAACTGGCCAAAGGAAGTAAACTTTGACCAACTCTCTTATGATTCACGAAAATCAACAGAAAATACCCTTTTCTTTG
CCAAGGGTTTGAATTTTAAAAAAGAATATTTAACAGACTTAGAAGCTGCTTTTTATGTTTCAGAATTTGATTATGAAGTA
GCTCTCCCAGCAATTATTGTCACTGATGTTAAACGAGCAATGGCTTTAATCGCTGCAAGCTTTTATGATTTTCCACAAAA
TAAACTCAAAACCTTAGCTTTGACTGGTACAAAAGGAAAAACCACTTCTGCTTATTTTGCTAAAGCAATACTTGACAAAA
TGAACGGTGGCAAAACTGCACTTTTATCTACTGCCCAAACAACTTTGGATGGAAAAAATTATTTTAAATCAGAATTGACA
ACTCCTGAAAGTCTTGATTTACTAGAAATGATGGCAAAAGCTCTGACAAATGGAATGACACATTTGGTCATGGAAGTTTC
AAGCCAAGCTTACAAAACAGAACGGGTTTACGGTTTAACATTTGATGTTGGAGTTTTCTTAAACATTTCTCCTGACCACA
TTGGCCCAGTTGAACATCCAACGCTTGAAGATTATTTTTACTGCAAACGTCAACTTCTCAAAAATTCACGTTATTTTGTT
GCTAATTCCGAAATGAATCACTTTGCTATCATTAAAGAGGAACTTAAGGAACGTAAAATTCCACATGCTTTCTATGGTGC
TGATTCTGAAAATAAAATTATTGAATCAAAAGGACTTCATTTTGTTACTGACGGTTCTGTCAGTGGAGCATTTGACATTC
GCTTATTAGGACGCTTCAACCAAGAAAATGCCTTAGCTACAGCATTAGCAACTAAAGCTTTAGGCGCTTCATTAGAAAAT
ATCCATGACGGTTTAGCTCTTGCTGTTGTTCCAGGACGCATGGAACTTCTTACTGCCAAAAATGGCGCTCACATCTATAT
TGATTATGCTCACAACGGCCTGTCATTAGAAAATCTTGTTGAAGTTGTTGAAGAACACCATGCTGGTCAGCTTGTTCTAG
TCCTTGGTTCAACCGGAAATAAAGGCGAAAGTCGCCGGAAAGATTTCGGACAAGTGATTGAAAAACATCCGCGACTTGAT
GTCATTTTAACTACTGATGATTCAAATCGAGAAGACCCTAAAATAATTGCTGACGAGATTGCTTCTTTTGTCAGTCGCAC
ACTTGATTTTGAACTTGACCGTGAATTGGCAATAAAAAAAGCTGTCAGTAAAACTAAAAGTACTGACGATGCTGTGATTA
TCGCTGGAAAAGGGACTGATATGTTCCAATTAAAAGACGGAAAACGTCAACCTTATATTGGAGATACAGCTGCTGCTCAA
AAATATCTCTAA

Upstream 100 bases:

>100_bases
CTCTTGGCTTGTGCTATAATATAGGGTAATGGGATTTTTCCCTTAGTTTCCTTGTTTTAGAAAGGTATCGCGAATTTAGC
GATAAATAGGCATTCCTCAC

Downstream 100 bases:

>100_bases
TTTACTGACAAAAGTTCTGTCAGTAAATTAATCATAAAAAAACGACTCAATAGGAGTCGTTTTTTCTTTTTCTTCTGACT
AGGCAAAAGAATTAACAACA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MIRLEQVIEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTENTLFFAKGLNFKKEYLTDLEAAFYVSEFDYEV
ALPAIIVTDVKRAMALIAASFYDFPQNKLKTLALTGTKGKTTSAYFAKAILDKMNGGKTALLSTAQTTLDGKNYFKSELT
TPESLDLLEMMAKALTNGMTHLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
ANSEMNHFAIIKEELKERKIPHAFYGADSENKIIESKGLHFVTDGSVSGAFDIRLLGRFNQENALATALATKALGASLEN
IHDGLALAVVPGRMELLTAKNGAHIYIDYAHNGLSLENLVEVVEEHHAGQLVLVLGSTGNKGESRRKDFGQVIEKHPRLD
VILTTDDSNREDPKIIADEIASFVSRTLDFELDRELAIKKAVSKTKSTDDAVIIAGKGTDMFQLKDGKRQPYIGDTAAAQ
KYL

Sequences:

>Translated_483_residues
MIRLEQVIEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTENTLFFAKGLNFKKEYLTDLEAAFYVSEFDYEV
ALPAIIVTDVKRAMALIAASFYDFPQNKLKTLALTGTKGKTTSAYFAKAILDKMNGGKTALLSTAQTTLDGKNYFKSELT
TPESLDLLEMMAKALTNGMTHLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
ANSEMNHFAIIKEELKERKIPHAFYGADSENKIIESKGLHFVTDGSVSGAFDIRLLGRFNQENALATALATKALGASLEN
IHDGLALAVVPGRMELLTAKNGAHIYIDYAHNGLSLENLVEVVEEHHAGQLVLVLGSTGNKGESRRKDFGQVIEKHPRLD
VILTTDDSNREDPKIIADEIASFVSRTLDFELDRELAIKKAVSKTKSTDDAVIIAGKGTDMFQLKDGKRQPYIGDTAAAQ
KYL
>Mature_483_residues
MIRLEQVIEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTENTLFFAKGLNFKKEYLTDLEAAFYVSEFDYEV
ALPAIIVTDVKRAMALIAASFYDFPQNKLKTLALTGTKGKTTSAYFAKAILDKMNGGKTALLSTAQTTLDGKNYFKSELT
TPESLDLLEMMAKALTNGMTHLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
ANSEMNHFAIIKEELKERKIPHAFYGADSENKIIESKGLHFVTDGSVSGAFDIRLLGRFNQENALATALATKALGASLEN
IHDGLALAVVPGRMELLTAKNGAHIYIDYAHNGLSLENLVEVVEEHHAGQLVLVLGSTGNKGESRRKDFGQVIEKHPRLD
VILTTDDSNREDPKIIADEIASFVSRTLDFELDRELAIKKAVSKTKSTDDAVIIAGKGTDMFQLKDGKRQPYIGDTAAAQ
KYL

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=498, Percent_Identity=26.5060240963855, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_LACLA (Q9CEN1)

Other databases:

- EMBL:   AE005176
- PIR:   F86850
- RefSeq:   NP_267963.1
- ProteinModelPortal:   Q9CEN1
- SMR:   Q9CEN1
- GeneID:   1115471
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L53929
- NMPDR:   fig|272623.1.peg.1852
- HOGENOM:   HBG351099
- OMA:   GHEEGQT
- ProtClustDB:   PRK00139
- BioCyc:   LLAC272623:L53929-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: 6.3.2.13

Molecular weight: Translated: 53927; Mature: 53927

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: BINDING 43-43 BINDING 187-187 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLEQVIEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTENTLFFAKGLNFK
CCCHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCH
KEYLTDLEAAFYVSEFDYEVALPAIIVTDVKRAMALIAASFYDFPQNKLKTLALTGTKGK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
TTSAYFAKAILDKMNGGKTALLSTAQTTLDGKNYFKSELTTPESLDLLEMMAKALTNGMT
CHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
HLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
HHHHHHHHHHHHHCEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
ANSEMNHFAIIKEELKERKIPHAFYGADSENKIIESKGLHFVTDGSVSGAFDIRLLGRFN
ECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCEEEECCCCCCEEEEEEEECCC
QENALATALATKALGASLENIHDGLALAVVPGRMELLTAKNGAHIYIDYAHNGLSLENLV
CCHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCEEEEEECCCCEEEEEECCCCCCHHHHH
EVVEEHHAGQLVLVLGSTGNKGESRRKDFGQVIEKHPRLDVILTTDDSNREDPKIIADEI
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHH
ASFVSRTLDFELDRELAIKKAVSKTKSTDDAVIIAGKGTDMFQLKDGKRQPYIGDTAAAQ
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHH
KYL
CCC
>Mature Secondary Structure
MIRLEQVIEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTENTLFFAKGLNFK
CCCHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCH
KEYLTDLEAAFYVSEFDYEVALPAIIVTDVKRAMALIAASFYDFPQNKLKTLALTGTKGK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
TTSAYFAKAILDKMNGGKTALLSTAQTTLDGKNYFKSELTTPESLDLLEMMAKALTNGMT
CHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
HLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
HHHHHHHHHHHHHCEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
ANSEMNHFAIIKEELKERKIPHAFYGADSENKIIESKGLHFVTDGSVSGAFDIRLLGRFN
ECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCEEEECCCCCCEEEEEEEECCC
QENALATALATKALGASLENIHDGLALAVVPGRMELLTAKNGAHIYIDYAHNGLSLENLV
CCHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCEEEEEECCCCEEEEEECCCCCCHHHHH
EVVEEHHAGQLVLVLGSTGNKGESRRKDFGQVIEKHPRLDVILTTDDSNREDPKIIADEI
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHH
ASFVSRTLDFELDRELAIKKAVSKTKSTDDAVIIAGKGTDMFQLKDGKRQPYIGDTAAAQ
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHH
KYL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 1.5 {meso-lanthionine}} 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471