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The map label for this gene is kdgA

Identifier: 15673608

GI number: 15673608

Start: 1671413

End: 1672054

Strand: Reverse

Name: kdgA

Synonym: L0022

Alternate gene names: 15673608

Gene position: 1672054-1671413 (Counterclockwise)

Preceding gene: 15673609

Following gene: 15673607

Centisome position: 70.68

GC content: 40.81

Gene sequence:

>642_bases
ATGCAAAGAGCAGAATTATTAAATAAAGTGATTCAATCAGGAGTCGTCTCGGTCGTCCGAGCTGAGAGTGGCGAAAAAGC
ATTAAAAATCGTAGAAGCTGTCATCTCAGGAGGAATCACTTCTATCGAATTGACCTATTCAGTCCCGCGAGCTAACGAAG
TTATTTCTGATTTGGTAGCCAAGTATCTAGGAACAGAAGTAGTGATCGGAGCAGGGACAGTTTTAGATCCGACCTCTGCT
CGTTTGGCCATTGTTGCAGGGGCTCAATTTATTGTCAGTCCAAGTTTTAACAAAGAAGTCGCAAAGATATGTAATCTTTA
TCAGATTCCCTATGTTCCGGGAATTTTAACTCCACTCGAAGCTCAAAAAGCTTTAGAATATGGGTCAGAGTTAATCAAAC
TTTTCCCAGGTGATATTACAGGTCCCAAAATGATAAAAGACTTAAAAGGTCCATTTCCTTATATTAATATCCTTCCATCG
GGAGGAGTAAATGTTGATAATGTTGCTGAATGGTTTGCAGCAGGAGCAGTAGCTGTAAGTGCTGGAGGTGGGGTTACAGC
TCCAGCAGCCAACAACAATTATAGTGGGGTTACTGAAAATGCCAAGAAATTTATGGCAGCATTTAAGGCAGCTAAGAAAT
AA

Upstream 100 bases:

>100_bases
GTGCCTGTGCCGTTGGAGCATTAGCAGTTCAAAGCTCAGGAGATAGTGAAGGTTATCCCACAAAAGAAGAATTAAAAGCA
TTTTACAAGGAGATGTAGTC

Downstream 100 bases:

>100_bases
AAATTAGCTATTAATTTTCCCTTTTGAATAATAAAAAACTTCATTTTTTGAAGTTTTTTTATTTTAAATATTTTTTTATA
AAACAGGTCAACCGCAACCT

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQRAELLNKVIQSGVVSVVRAESGEKALKIVEAVISGGITSIELTYSVPRANEVISDLVAKYLGTEVVIGAGTVLDPTSA
RLAIVAGAQFIVSPSFNKEVAKICNLYQIPYVPGILTPLEAQKALEYGSELIKLFPGDITGPKMIKDLKGPFPYINILPS
GGVNVDNVAEWFAAGAVAVSAGGGVTAPAANNNYSGVTENAKKFMAAFKAAKK

Sequences:

>Translated_213_residues
MQRAELLNKVIQSGVVSVVRAESGEKALKIVEAVISGGITSIELTYSVPRANEVISDLVAKYLGTEVVIGAGTVLDPTSA
RLAIVAGAQFIVSPSFNKEVAKICNLYQIPYVPGILTPLEAQKALEYGSELIKLFPGDITGPKMIKDLKGPFPYINILPS
GGVNVDNVAEWFAAGAVAVSAGGGVTAPAANNNYSGVTENAKKFMAAFKAAKK
>Mature_213_residues
MQRAELLNKVIQSGVVSVVRAESGEKALKIVEAVISGGITSIELTYSVPRANEVISDLVAKYLGTEVVIGAGTVLDPTSA
RLAIVAGAQFIVSPSFNKEVAKICNLYQIPYVPGILTPLEAQKALEYGSELIKLFPGDITGPKMIKDLKGPFPYINILPS
GGVNVDNVAEWFAAGAVAVSAGGGVTAPAANNNYSGVTENAKKFMAAFKAAKK

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=198, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788156, Length=183, Percent_Identity=30.0546448087432, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22238; Mature: 22238

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRAELLNKVIQSGVVSVVRAESGEKALKIVEAVISGGITSIELTYSVPRANEVISDLVA
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHH
KYLGTEVVIGAGTVLDPTSARLAIVAGAQFIVSPSFNKEVAKICNLYQIPYVPGILTPLE
HHCCCEEEEECCCEECCCCCEEEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHH
AQKALEYGSELIKLFPGDITGPKMIKDLKGPFPYINILPSGGVNVDNVAEWFAAGAVAVS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEE
AGGGVTAPAANNNYSGVTENAKKFMAAFKAAKK
CCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQRAELLNKVIQSGVVSVVRAESGEKALKIVEAVISGGITSIELTYSVPRANEVISDLVA
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHH
KYLGTEVVIGAGTVLDPTSARLAIVAGAQFIVSPSFNKEVAKICNLYQIPYVPGILTPLE
HHCCCEEEEECCCEECCCCCEEEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHH
AQKALEYGSELIKLFPGDITGPKMIKDLKGPFPYINILPSGGVNVDNVAEWFAAGAVAVS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEE
AGGGVTAPAANNNYSGVTENAKKFMAAFKAAKK
CCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]