Click here to switch to the map view.

The map label for this gene is dapA

Identifier: 15673602

GI number: 15673602

Start: 1664751

End: 1665644

Strand: Reverse

Name: dapA

Synonym: L0093

Alternate gene names: 15673602

Gene position: 1665644-1664751 (Counterclockwise)

Preceding gene: 15673603

Following gene: 15673600

Centisome position: 70.41

GC content: 37.81

Gene sequence:

>894_bases
ATGTCAGCAAAAGAAACAATTGAAAAATTACAAAATGCACGTATCATCACGGCCCTAGTGACCCCTTTTAAAGAAAATGG
CCAAATTAATTTTGGAGCATTTCCTAAGCTCATTGAGGATTTACTTGCTAATCATACGGAAGGCCTTATCTTAGCTGGAA
CAACGGCTGAATCGCCTACTCTTACTCATGATGAAGAATTGGCAATTTTTGCTGCGGTTAATAAAATAGTGGATGGCCGT
ATTCCATTGATTGCAGGTGTTGGGACTAATGACACACGTGATTCGGTTGAATTTGTCAAAGAAGTTGCTGAACTTGGTTA
CATCGATGCGGGACTTGCAGTGACTCCTTATTACAACAAACCTTCTCAAGAAGGAATTTATCAACATTTTAAAGCCATTG
CTACTGCGAGTGATTTGCCAATTATTTTGTATAATATTCCAGGAAGGGTGGTCACTGAAATTCAGGTAGAGACCATCTTA
CGATTAGCAGAACTGGAAAATGTAATTGCCATCAAAGAATGTACAAATACTGATAATCTGGCTTATCTCATTGAAAAGCT
ACCCAAAGACTTTTTAGTTTATACTGGAGAAGATGGGCTAGCTTTTCACACTAAAGCTTTGGGAGGTCAGGGTGTAATCT
CGGTTGCCAGTCATATCCTAGGTCAAGAATTTTTTGAAATGTTTGCAGAAATAGACCAGGGCTCTATTCAAAAAGCAGCG
GCTATCCAAAGAAAAATATTACCCAAAATAAATGCACTTTTTAGTGTCACAAGTCCGGCTCCAATCAAAACGGTTTTGAA
TGCAAAAGGATATGAAGTTGGTGGCTTACGTTTACCACTTGTAGCTTGTACTACTGAGGAAAGTAAAATAATTCTTGAAA
AAATTGGAAATTAA

Upstream 100 bases:

>100_bases
AATTTTTGACGAACTTTTGAGAACCTCTTTTGCATAAAAAGAGGTTCTTTTTGACCTCTTGACAAGAGCTTGTCAGTAAG
CTCACACATAAGGAGAAATT

Downstream 100 bases:

>100_bases
AAAATACCCTTTTAGGGTATTTTTCTTTATAAAAGTAGGTCAATATTCAATTGATTTAATCTTATCATTCGATTCCAAAA
GACTCATTAACGAAGCGACT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSAKETIEKLQNARIITALVTPFKENGQINFGAFPKLIEDLLANHTEGLILAGTTAESPTLTHDEELAIFAAVNKIVDGR
IPLIAGVGTNDTRDSVEFVKEVAELGYIDAGLAVTPYYNKPSQEGIYQHFKAIATASDLPIILYNIPGRVVTEIQVETIL
RLAELENVIAIKECTNTDNLAYLIEKLPKDFLVYTGEDGLAFHTKALGGQGVISVASHILGQEFFEMFAEIDQGSIQKAA
AIQRKILPKINALFSVTSPAPIKTVLNAKGYEVGGLRLPLVACTTEESKIILEKIGN

Sequences:

>Translated_297_residues
MSAKETIEKLQNARIITALVTPFKENGQINFGAFPKLIEDLLANHTEGLILAGTTAESPTLTHDEELAIFAAVNKIVDGR
IPLIAGVGTNDTRDSVEFVKEVAELGYIDAGLAVTPYYNKPSQEGIYQHFKAIATASDLPIILYNIPGRVVTEIQVETIL
RLAELENVIAIKECTNTDNLAYLIEKLPKDFLVYTGEDGLAFHTKALGGQGVISVASHILGQEFFEMFAEIDQGSIQKAA
AIQRKILPKINALFSVTSPAPIKTVLNAKGYEVGGLRLPLVACTTEESKIILEKIGN
>Mature_296_residues
SAKETIEKLQNARIITALVTPFKENGQINFGAFPKLIEDLLANHTEGLILAGTTAESPTLTHDEELAIFAAVNKIVDGRI
PLIAGVGTNDTRDSVEFVKEVAELGYIDAGLAVTPYYNKPSQEGIYQHFKAIATASDLPIILYNIPGRVVTEIQVETILR
LAELENVIAIKECTNTDNLAYLIEKLPKDFLVYTGEDGLAFHTKALGGQGVISVASHILGQEFFEMFAEIDQGSIQKAAA
IQRKILPKINALFSVTSPAPIKTVLNAKGYEVGGLRLPLVACTTEESKIILEKIGN

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=277, Percent_Identity=24.5487364620939, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI13540533, Length=292, Percent_Identity=26.027397260274, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788823, Length=271, Percent_Identity=39.1143911439114, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082415, Length=284, Percent_Identity=26.7605633802817, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786463, Length=250, Percent_Identity=26.8, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789620, Length=254, Percent_Identity=27.1653543307087, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_LACLA (Q9CF61)

Other databases:

- EMBL:   AE005176
- PIR:   D86827
- RefSeq:   NP_267776.1
- ProteinModelPortal:   Q9CF61
- SMR:   Q9CF61
- GeneID:   1115280
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0093
- NMPDR:   fig|272623.1.peg.1662
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- ProtClustDB:   PRK03170
- BioCyc:   LLAC272623:L0093-MONOMER
- BRENDA:   4.2.1.52
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32190; Mature: 32059

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 172-172 BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKETIEKLQNARIITALVTPFKENGQINFGAFPKLIEDLLANHTEGLILAGTTAESPT
CCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
LTHDEELAIFAAVNKIVDGRIPLIAGVGTNDTRDSVEFVKEVAELGYIDAGLAVTPYYNK
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCC
PSQEGIYQHFKAIATASDLPIILYNIPGRVVTEIQVETILRLAELENVIAIKECTNTDNL
CCHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHEEEEEECCCCCHH
AYLIEKLPKDFLVYTGEDGLAFHTKALGGQGVISVASHILGQEFFEMFAEIDQGSIQKAA
HHHHHHCCCCCEEEECCCCCEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AIQRKILPKINALFSVTSPAPIKTVLNAKGYEVGGLRLPLVACTTEESKIILEKIGN
HHHHHHHHHHHHHEECCCCCCHHHHHCCCCCEECCEEEEEEEECCCCHHHHHHHCCC
>Mature Secondary Structure 
SAKETIEKLQNARIITALVTPFKENGQINFGAFPKLIEDLLANHTEGLILAGTTAESPT
CHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
LTHDEELAIFAAVNKIVDGRIPLIAGVGTNDTRDSVEFVKEVAELGYIDAGLAVTPYYNK
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCC
PSQEGIYQHFKAIATASDLPIILYNIPGRVVTEIQVETILRLAELENVIAIKECTNTDNL
CCHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHEEEEEECCCCCHH
AYLIEKLPKDFLVYTGEDGLAFHTKALGGQGVISVASHILGQEFFEMFAEIDQGSIQKAA
HHHHHHCCCCCEEEECCCCCEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AIQRKILPKINALFSVTSPAPIKTVLNAKGYEVGGLRLPLVACTTEESKIILEKIGN
HHHHHHHHHHHHHEECCCCCCHHHHHCCCCCEECCEEEEEEEECCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471