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The map label for this gene is gidB

Identifier: 15673325

GI number: 15673325

Start: 1380747

End: 1381463

Strand: Direct

Name: gidB

Synonym: L180469

Alternate gene names: 15673325

Gene position: 1380747-1381463 (Clockwise)

Preceding gene: 15673324

Following gene: 30024037

Centisome position: 58.37

GC content: 34.45

Gene sequence:

>717_bases
ATGACCCCTGATGAATTTTTGTCAGCACTGACAGAATTTGATATTCAACTCTCTGATAAACAAATCAAACAGTTTGAACG
CTACTTTGAACTCTTGGTTGAATGGAATGAAAAAATTAACTTGACAGCAATTACTGAAAAAAATGAAGTCTATTTAAAAC
ATTTTTATGATTCTATTGCACCAATTTTATATGGATTGATTACTGACCAACCTGTCAGCATTTTAGATATTGGTGCTGGA
GCTGGTTTTCCTAGCTTACCAATGAAAATTATTTTCCCTGAATTAAAAGTGACTATCATTGATTCTCTCAATAAGCGAAT
TAATTTTTTAAGTTTACTGACAGAGGAATTAGGCCTCGAAAATGTGACTTTACTTCACGGACGAGCTGAAGATTTTGGTC
AGGATGCTAATTATCGTGGAACTTTTGATTTTGTTACTGCCCGTGCGGTTGCTAGACTTTCTGTTTTGACTGAATTTACG
ATTCCCTTTCTCAAGAAAAATGGTATTTTATTGTCATTAAAAGCAGCTCAATTTGAAGAAGAATTGAATGACGCTAAAAA
GGCAATTGCTACACTTGGTGGAAAATTTATCAAAGAAGTCGCTTATGAATTGCCTAACGGTGATGAGCGTCACATTGCTC
TGATTGAAAAGAAAAAAGAAACCCCTAAAAAATACCCACGCAAAGCCGGAACTCCTGCAAAAAATCCAATAAAATAA

Upstream 100 bases:

>100_bases
CTTATACTCTTTCAGCATTAAATTTAATTGATAAATTACCAAAAGGTAAAAATCGTGAGCTGATTGCTGAAATTACTAGA
AAACTTTTGGAGCGAACTTT

Downstream 100 bases:

>100_bases
AAAAATCGGCCATTTTTAGCCGATTTTTTTGCTGAGATTAATCACCAATACTGAAATAGAAACAATCGCAGTAAAACTAT
TCATGACCATTGCAGGTGCC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTPDEFLSALTEFDIQLSDKQIKQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSIAPILYGLITDQPVSILDIGAG
AGFPSLPMKIIFPELKVTIIDSLNKRINFLSLLTEELGLENVTLLHGRAEDFGQDANYRGTFDFVTARAVARLSVLTEFT
IPFLKKNGILLSLKAAQFEEELNDAKKAIATLGGKFIKEVAYELPNGDERHIALIEKKKETPKKYPRKAGTPAKNPIK

Sequences:

>Translated_238_residues
MTPDEFLSALTEFDIQLSDKQIKQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSIAPILYGLITDQPVSILDIGAG
AGFPSLPMKIIFPELKVTIIDSLNKRINFLSLLTEELGLENVTLLHGRAEDFGQDANYRGTFDFVTARAVARLSVLTEFT
IPFLKKNGILLSLKAAQFEEELNDAKKAIATLGGKFIKEVAYELPNGDERHIALIEKKKETPKKYPRKAGTPAKNPIK
>Mature_237_residues
TPDEFLSALTEFDIQLSDKQIKQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSIAPILYGLITDQPVSILDIGAGA
GFPSLPMKIIFPELKVTIIDSLNKRINFLSLLTEELGLENVTLLHGRAEDFGQDANYRGTFDFVTARAVARLSVLTEFTI
PFLKKNGILLSLKAAQFEEELNDAKKAIATLGGKFIKEVAYELPNGDERHIALIEKKKETPKKYPRKAGTPAKNPIK

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=215, Percent_Identity=33.4883720930233, Blast_Score=107, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_LACLA (Q9CFX1)

Other databases:

- EMBL:   AE005176
- PIR:   G86792
- RefSeq:   NP_267499.1
- ProteinModelPortal:   Q9CFX1
- SMR:   Q9CFX1
- GeneID:   1114993
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L180469
- NMPDR:   fig|272623.1.peg.1378
- HOGENOM:   HBG686577
- OMA:   YELLVEW
- ProtClustDB:   PRK00107
- BioCyc:   LLAC272623:L180469-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 26892; Mature: 26761

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDEFLSALTEFDIQLSDKQIKQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSIA
CCHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHH
PILYGLITDQPVSILDIGAGAGFPSLPMKIIFPELKVTIIDSLNKRINFLSLLTEELGLE
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVTLLHGRAEDFGQDANYRGTFDFVTARAVARLSVLTEFTIPFLKKNGILLSLKAAQFEE
CEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
ELNDAKKAIATLGGKFIKEVAYELPNGDERHIALIEKKKETPKKYPRKAGTPAKNPIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPDEFLSALTEFDIQLSDKQIKQFERYFELLVEWNEKINLTAITEKNEVYLKHFYDSIA
CHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHH
PILYGLITDQPVSILDIGAGAGFPSLPMKIIFPELKVTIIDSLNKRINFLSLLTEELGLE
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVTLLHGRAEDFGQDANYRGTFDFVTARAVARLSVLTEFTIPFLKKNGILLSLKAAQFEE
CEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
ELNDAKKAIATLGGKFIKEVAYELPNGDERHIALIEKKKETPKKYPRKAGTPAKNPIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471