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The map label for this gene is ispB

Identifier: 15673324

GI number: 15673324

Start: 1379791

End: 1380750

Strand: Direct

Name: ispB

Synonym: L179531

Alternate gene names: 15673324

Gene position: 1379791-1380750 (Clockwise)

Preceding gene: 15673323

Following gene: 15673325

Centisome position: 58.33

GC content: 33.54

Gene sequence:

>960_bases
ATGGACTTAAAAAATAACTTTTGGAAAGACTATCCAGAGCTTGAAAAAGAATTGGTTCAAGTTCAACAATTAATGAAAAG
CCACGTTACCATCAAAAATTCAGATGTTAAATCGGCTATTTTTGATATATTTGATGCGGGCGGAAAAATGTTGCGTCCAG
CTTATTTACTTTTATTTTCAGGATTTACTGATCTTTCTGAAAGTGAAAGATTGGCACTTGCGGCTTCTGTCGAAATGATG
CATACAGCGACTTTGGTTCACGATGATGTCATTGACCACGCAGACACACGGCGTGGCGTAGCTACTTTGTCAGCAAAATA
CGGACCAGAAATTGCCGTCTATGCTGGGGACTATTTATTTATTGCTGTCTTTCGTCTAATGTCTGAACACTCATTAGAAT
TATCAAATTTAACCAAAAATATTGGTTCAATTGAGCGTTTATTAGGTGGTGAGCTTGGTCAACTTAATCATCATTTTAAT
CTTCAACAAACTTTAGATGATTATATTGAAAATATTTCAGGAAAAACGGGAGAACTTTTTGCACTTTCTGCTTCTGTTGC
TCCTTTAATTTCTAAAAATAATACATTGACCAAACGAGCTTATAAAATTGGAATGAATATTGGGATTTCTTTCCAAATTA
TGGATGATTATTTAGATTATGCATCGACTGCTCAAACGCTTGGAAAACCGGTACTTGAAGACATTAAACAAGGCATTTAT
TCTGCTCCTGTTCTCTTTGCTTTACAGGAAAACAATGCTCTTGTTTCTGAATTAATTAAAAATGAAAAATTTGATGAAGT
TTATGATTTTATCAAAACTTCTGATGCCCTTGAAAAAACCAAAGCACTTGCAAAATCTTATACTCTTTCAGCATTAAATT
TAATTGATAAATTACCAAAAGGTAAAAATCGTGAGCTGATTGCTGAAATTACTAGAAAACTTTTGGAGCGAACTTTATGA

Upstream 100 bases:

>100_bases
TTTTTGGAATACTTGCTGGTTTTGCAATTGGCATTGCTGGAAATCAATTAATTACACGTGTTAAACCTATTCAAGAACTA
TTTATTAAAGAAAGTAAACA

Downstream 100 bases:

>100_bases
CCCCTGATGAATTTTTGTCAGCACTGACAGAATTTGATATTCAACTCTCTGATAAACAAATCAAACAGTTTGAACGCTAC
TTTGAACTCTTGGTTGAATG

Product: heptaprenyl diphosphate synthase component II

Products: NA

Alternate protein names: HEPPP synthase subunit 2; Spore germination protein C3 [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MDLKNNFWKDYPELEKELVQVQQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFSGFTDLSESERLALAASVEMM
HTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLFIAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFN
LQQTLDDYIENISGKTGELFALSASVAPLISKNNTLTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPKGKNRELIAEITRKLLERTL

Sequences:

>Translated_319_residues
MDLKNNFWKDYPELEKELVQVQQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFSGFTDLSESERLALAASVEMM
HTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLFIAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFN
LQQTLDDYIENISGKTGELFALSASVAPLISKNNTLTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPKGKNRELIAEITRKLLERTL
>Mature_319_residues
MDLKNNFWKDYPELEKELVQVQQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFSGFTDLSESERLALAASVEMM
HTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLFIAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFN
LQQTLDDYIENISGKTGELFALSASVAPLISKNNTLTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPKGKNRELIAEITRKLLERTL

Specific function: Supplies heptaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinone menaquinone-7 (MQ-7) [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=330, Percent_Identity=32.4242424242424, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4758430, Length=273, Percent_Identity=24.9084249084249, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI83700220, Length=273, Percent_Identity=24.9084249084249, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789578, Length=298, Percent_Identity=27.1812080536913, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786623, Length=237, Percent_Identity=29.957805907173, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17505681, Length=292, Percent_Identity=34.2465753424658, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=378, Percent_Identity=29.8941798941799, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6325188, Length=209, Percent_Identity=25.8373205741627, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24651612, Length=311, Percent_Identity=29.903536977492, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI281365769, Length=256, Percent_Identity=28.90625, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24660002, Length=256, Percent_Identity=28.90625, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014119
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.30 [H]

Molecular weight: Translated: 35676; Mature: 35676

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKNNFWKDYPELEKELVQVQQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFS
CCCCCCHHCCCHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHH
GFTDLSESERLALAASVEMMHTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECHHHH
IAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFNLQQTLDDYIENISGKTGELF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCEE
ALSASVAPLISKNNTLTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
EEECHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHH
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPK
CCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKNRELIAEITRKLLERTL
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLKNNFWKDYPELEKELVQVQQLMKSHVTIKNSDVKSAIFDIFDAGGKMLRPAYLLLFS
CCCCCCHHCCCHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHH
GFTDLSESERLALAASVEMMHTATLVHDDVIDHADTRRGVATLSAKYGPEIAVYAGDYLF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECHHHH
IAVFRLMSEHSLELSNLTKNIGSIERLLGGELGQLNHHFNLQQTLDDYIENISGKTGELF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCEE
ALSASVAPLISKNNTLTKRAYKIGMNIGISFQIMDDYLDYASTAQTLGKPVLEDIKQGIY
EEECHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHH
SAPVLFALQENNALVSELIKNEKFDEVYDFIKTSDALEKTKALAKSYTLSALNLIDKLPK
CCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKNRELIAEITRKLLERTL
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 2121900; 9720033 [H]