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The map label for this gene is amyL

Identifier: 15673233

GI number: 15673233

Start: 1277443

End: 1278918

Strand: Direct

Name: amyL

Synonym: L77437

Alternate gene names: 15673233

Gene position: 1277443-1278918 (Clockwise)

Preceding gene: 15673225

Following gene: 15673249

Centisome position: 54.0

GC content: 33.2

Gene sequence:

>1476_bases
ATGAAAACAATCCTTCAAGCTTTTGAATGGTATTTACCCTCAGATTCTCAGCACTGGAATAATATAAAAGAGAATATTCC
TGACTTGGGAAAGTTAGGATTTTCAGGATTGTGGTTACCTCCAGCTTCCAAGGCAGCTTCTGGAGTTGAAGATGTTGGTT
ATGGGACCTATGATTTATTCGACTTAGGGGAATTTGATCAAAAAGGGACAATTCCTACAAAATATGGGACTAAAGATGAA
TATCTTGATTTAATTAATACACTACACCACAACAATATTGAAGTGTATGCGGATATCGTTTTTAATCACATGATGGGTGC
TGATGAAACAGAAACTATTGAAGCTGATATTAAAGCAGAAGATAATCATCTTCATAACATCGAAAATAATAAAACTGTTG
AAGTTTGGACCAAATTTACATTTCCAGGAAGACAAGGAAAATACGACAATTATATATGGACCTGGCACAACTTTACGGGA
ATTGATTATGATGAAAGGAAAAATCAAGAAGAAATTCTTGAATTTGAAGGACATGAATGGGATGAGAATGTAGATTCAGA
GAATAATAATTTTGATTATTTAATGGGGGCTGATCTTGATTTTAGTGTTTCGGAAACGGTAGAACAACTTGAAAAATGGG
GACATTGGTTTTCAGAAATGACAAAAATTGATGGTTTTCGATTAGATGCAATTAAACATATTGATTTCAAATATTTTGAT
AAATGGCTTGAGCAGAGAGCAAAGCAATTGGATAGAAAACTTTTTATTGTTGGCGAGTACTGGTCTGATGATCTTGGCAA
ATTAGAGTATTATCTAGAACAATCCAGTGATAGAATACAATTATTTGATGTACCACTACATTTTAATATGAAAGAAGCCT
CTTCAACCAATGGAGAATTTGATATGCGTACTCTTTTTGATCACACACTCACTGCCAGCCAGCCAGAACTCTCGGTCACT
TTTGTTGATAACCATGATACTCAAGAAGGACAGGCTCTTCAATCTTGGATTCCAGCTTGGTTTAAAGAGCACGCTTATAG
CTTAATTTTACTTCGAAAAAAAGAAACTCCTACTGTCTTTTGGGGAGATTTATATGGAATACCCTCACATAATGTTAATC
CTGTAGGTGATAACTTAAGAACTATGATAGCATTACGTAAGGACTCTGAGTTTTTAAGGGAAAATGATTATTTTGATCAT
CCTGATATTATTGGATGGACAAATATTTTAAAAATAGATAATAAGGAATATGGATTATCATGTATTTTAACTAATAAAAA
TGGTGGCTCAAAATATATGATAATTGATAAGGCGTATGCAGGTAAAGTTTATATAGATTTATTTGGAAGACACGAAATAC
CTATTACGCTGGATCAAAATGGTGGAGCTGAATTTTATGTCAATGATGGAAGTGTTTCGGTTTGGGTAGATAAGGAAATT
GTTTCAAAAATTGATCAAATGAACTTTCAAAACTAA

Upstream 100 bases:

>100_bases
CATTCTGCTGTCCTTCAGATTCCTTTTTGTGTATACTAATTATTAATAGCGCTAGTTTTTAGAAAAGAATGTATCAATTT
GAAAAAGGAGAAATGATTTT

Downstream 100 bases:

>100_bases
CTATTTTATCAAATAGGATAAGGATAAAAAAAGTACCTCTAATAAGGTACTTTTTTATTTGAACTATTAGTCAATCAATG
AGGTATGTTTGATTTCTTCA

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKTILQAFEWYLPSDSQHWNNIKENIPDLGKLGFSGLWLPPASKAASGVEDVGYGTYDLFDLGEFDQKGTIPTKYGTKDE
YLDLINTLHHNNIEVYADIVFNHMMGADETETIEADIKAEDNHLHNIENNKTVEVWTKFTFPGRQGKYDNYIWTWHNFTG
IDYDERKNQEEILEFEGHEWDENVDSENNNFDYLMGADLDFSVSETVEQLEKWGHWFSEMTKIDGFRLDAIKHIDFKYFD
KWLEQRAKQLDRKLFIVGEYWSDDLGKLEYYLEQSSDRIQLFDVPLHFNMKEASSTNGEFDMRTLFDHTLTASQPELSVT
FVDNHDTQEGQALQSWIPAWFKEHAYSLILLRKKETPTVFWGDLYGIPSHNVNPVGDNLRTMIALRKDSEFLRENDYFDH
PDIIGWTNILKIDNKEYGLSCILTNKNGGSKYMIIDKAYAGKVYIDLFGRHEIPITLDQNGGAEFYVNDGSVSVWVDKEI
VSKIDQMNFQN

Sequences:

>Translated_491_residues
MKTILQAFEWYLPSDSQHWNNIKENIPDLGKLGFSGLWLPPASKAASGVEDVGYGTYDLFDLGEFDQKGTIPTKYGTKDE
YLDLINTLHHNNIEVYADIVFNHMMGADETETIEADIKAEDNHLHNIENNKTVEVWTKFTFPGRQGKYDNYIWTWHNFTG
IDYDERKNQEEILEFEGHEWDENVDSENNNFDYLMGADLDFSVSETVEQLEKWGHWFSEMTKIDGFRLDAIKHIDFKYFD
KWLEQRAKQLDRKLFIVGEYWSDDLGKLEYYLEQSSDRIQLFDVPLHFNMKEASSTNGEFDMRTLFDHTLTASQPELSVT
FVDNHDTQEGQALQSWIPAWFKEHAYSLILLRKKETPTVFWGDLYGIPSHNVNPVGDNLRTMIALRKDSEFLRENDYFDH
PDIIGWTNILKIDNKEYGLSCILTNKNGGSKYMIIDKAYAGKVYIDLFGRHEIPITLDQNGGAEFYVNDGSVSVWVDKEI
VSKIDQMNFQN
>Mature_491_residues
MKTILQAFEWYLPSDSQHWNNIKENIPDLGKLGFSGLWLPPASKAASGVEDVGYGTYDLFDLGEFDQKGTIPTKYGTKDE
YLDLINTLHHNNIEVYADIVFNHMMGADETETIEADIKAEDNHLHNIENNKTVEVWTKFTFPGRQGKYDNYIWTWHNFTG
IDYDERKNQEEILEFEGHEWDENVDSENNNFDYLMGADLDFSVSETVEQLEKWGHWFSEMTKIDGFRLDAIKHIDFKYFD
KWLEQRAKQLDRKLFIVGEYWSDDLGKLEYYLEQSSDRIQLFDVPLHFNMKEASSTNGEFDMRTLFDHTLTASQPELSVT
FVDNHDTQEGQALQSWIPAWFKEHAYSLILLRKKETPTVFWGDLYGIPSHNVNPVGDNLRTMIALRKDSEFLRENDYFDH
PDIIGWTNILKIDNKEYGLSCILTNKNGGSKYMIIDKAYAGKVYIDLFGRHEIPITLDQNGGAEFYVNDGSVSVWVDKEI
VSKIDQMNFQN

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=494, Percent_Identity=39.6761133603239, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 57200; Mature: 57200

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTILQAFEWYLPSDSQHWNNIKENIPDLGKLGFSGLWLPPASKAASGVEDVGYGTYDLF
CHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHCCCCCEECCCCHHHHCCHHHCCCCCEEEC
DLGEFDQKGTIPTKYGTKDEYLDLINTLHHNNIEVYADIVFNHMMGADETETIEADIKAE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCEEEEEEECC
DNHLHNIENNKTVEVWTKFTFPGRQGKYDNYIWTWHNFTGIDYDERKNQEEILEFEGHEW
CCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHCCHHHHHHCCCCCC
DENVDSENNNFDYLMGADLDFSVSETVEQLEKWGHWFSEMTKIDGFRLDAIKHIDFKYFD
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCHHHHH
KWLEQRAKQLDRKLFIVGEYWSDDLGKLEYYLEQSSDRIQLFDVPLHFNMKEASSTNGEF
HHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECEEECCHHCCCCCCCE
DMRTLFDHTLTASQPELSVTFVDNHDTQEGQALQSWIPAWFKEHAYSLILLRKKETPTVF
EHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCEEE
WGDLYGIPSHNVNPVGDNLRTMIALRKDSEFLRENDYFDHPDIIGWTNILKIDNKEYGLS
EECCCCCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEE
CILTNKNGGSKYMIIDKAYAGKVYIDLFGRHEIPITLDQNGGAEFYVNDGSVSVWVDKEI
EEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCCEEEEEECCEEEEEECHHH
VSKIDQMNFQN
HHHHHHCCCCC
>Mature Secondary Structure
MKTILQAFEWYLPSDSQHWNNIKENIPDLGKLGFSGLWLPPASKAASGVEDVGYGTYDLF
CHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHCCCCCEECCCCHHHHCCHHHCCCCCEEEC
DLGEFDQKGTIPTKYGTKDEYLDLINTLHHNNIEVYADIVFNHMMGADETETIEADIKAE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCEEEEEEECC
DNHLHNIENNKTVEVWTKFTFPGRQGKYDNYIWTWHNFTGIDYDERKNQEEILEFEGHEW
CCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHCCHHHHHHCCCCCC
DENVDSENNNFDYLMGADLDFSVSETVEQLEKWGHWFSEMTKIDGFRLDAIKHIDFKYFD
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCHHHHH
KWLEQRAKQLDRKLFIVGEYWSDDLGKLEYYLEQSSDRIQLFDVPLHFNMKEASSTNGEF
HHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECEEECCHHCCCCCCCE
DMRTLFDHTLTASQPELSVTFVDNHDTQEGQALQSWIPAWFKEHAYSLILLRKKETPTVF
EHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCEEE
WGDLYGIPSHNVNPVGDNLRTMIALRKDSEFLRENDYFDHPDIIGWTNILKIDNKEYGLS
EECCCCCCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCEE
CILTNKNGGSKYMIIDKAYAGKVYIDLFGRHEIPITLDQNGGAEFYVNDGSVSVWVDKEI
EEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCEEEECCCCEEEEEECCEEEEEECHHH
VSKIDQMNFQN
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924897; 3876333; 3009417; 2999073; 11226887 [H]