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The map label for this gene is gidC

Identifier: 15673213

GI number: 15673213

Start: 1256769

End: 1258112

Strand: Direct

Name: gidC

Synonym: L56416

Alternate gene names: 15673213

Gene position: 1256769-1258112 (Clockwise)

Preceding gene: 15673212

Following gene: 15673217

Centisome position: 53.13

GC content: 37.35

Gene sequence:

>1344_bases
ATGAAAAAAACACATATTAACGTTATAGGAGCGGGACTTGCTGGTTCTGAAGCTGCTTATCAAATTGCTAAGCGTGGCAT
TCCCGTTAAACTATACGAAATGCGAGGCGTTAAACAAACCCCTCAACACAAAACAGATAAATTTGCTGAACTAGTCTGTT
CAAATTCTCTTCGTGGAGCTGCAATCACTAACGCGGTTGGTCTCTTAAAAGAAGAAATGAGAAGACTTGATTCTGTAATT
ATCAAGGCAGCGGAAGTGACTCAAGTACCTGCTGGAGGAGCCTTAGCTGTTGATAGAGAAGGTTTCTCTGATTTTGTCAC
AAGAGAAGTTTCTAACCATCCATTGGTTGAAGTAATTCGTGAAGAAATTACTGATCTTCCAGAAGATGAAATTACAATTA
TTGCTACTGGACCTCTAACTTCAGATAAATTAGCAGAAAAAATTCATGAGTTTAACGGTGCGGATGGCTTTTATTTTTAT
GATGCAGCAGCACCAATTATTGACGCCAACTCGATTAATTTTGATAAAGTCTATAAAAAATCTCGATATGATAAAGGTGA
GGCTGACTATATCAACTGTCCAATGACCAAGGAAGAATTTCAAGCCTTTCAAGAAGCTTTAATTTCTGCTGAAGAAGCTC
CTTTAAACTCTTTTGAAGATTTAAAAGTCTTTGAAGGTTGTATGCCTATTGAAGAAATGGCCAAGCGTGGTTATAAAACG
ATGCTATTTGGCCCGATGAAACCTGTTGGATTGGAATATCCTGAAGATTATAAAGGCCCCAGAGATGGTGATTTTAAGAC
TCCCTATGCAGTTGTTCAACTTCGCCAAGATAATGCTTCCGCATCTTTGTACAATATCGTAGGATTTCAAACTCATTTAA
AATGGGGAGAACAAAAACGAGTTTTCCAAATGATTCCAGGTCTTGAAAATGCTGAATTCGTTCGTTATGGAGTCATGCAT
CGTAATTCTTACATGGATTCTCCAAATCTTCTTAAACAAACTTTCCAATCTCGCAAACAAGAGAATCTGTTCTTTGCAGG
ACAAATGACAGGAGTAGAGGGCTATGTAGAATCAGCTGCTTCTGGTTTAGTTGCTGGAATTAATGCTGTCAAACTCTTTA
ATGACGAAGAAGTAGTTATATTCCCAAAAACAACGGCGATTGGAAGTTTACCTTACTACATCACCCATACTGACAGCAAA
CATTTTCAACCAATGAATGTAACCTTCGGAATTGTTGAAGAATTAGAAGGCCCACGAATTCGTGATAAAAAAGAACGTTA
TACTAAGGTAGCAGAGCGTGCTTTAAATAGCTTAACAGATATTATTTCAAAAGATAATCTATAA

Upstream 100 bases:

>100_bases
GTGATTATCAAGAAGAAAAACAAAAATAAAAGGCGCTTCCGCGCTTTTTTATTATATTAATCAATGGAAAAATTTACTAA
ATTTAGATATAATTGGGACT

Downstream 100 bases:

>100_bases
CAAATAAAAAACACTTGGAATTTGAATCAAGTGTTTTTTATTTGTTATAGATTATCTAGAGCATTTTTTTGTTCTTCATT
GATAATATGAGTATAAAGGT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKQTPQHKTDKFAELVCSNSLRGAAITNAVGLLKEEMRRLDSVI
IKAAEVTQVPAGGALAVDREGFSDFVTREVSNHPLVEVIREEITDLPEDEITIIATGPLTSDKLAEKIHEFNGADGFYFY
DAAAPIIDANSINFDKVYKKSRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
MLFGPMKPVGLEYPEDYKGPRDGDFKTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMH
RNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAASGLVAGINAVKLFNDEEVVIFPKTTAIGSLPYYITHTDSK
HFQPMNVTFGIVEELEGPRIRDKKERYTKVAERALNSLTDIISKDNL

Sequences:

>Translated_447_residues
MKKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKQTPQHKTDKFAELVCSNSLRGAAITNAVGLLKEEMRRLDSVI
IKAAEVTQVPAGGALAVDREGFSDFVTREVSNHPLVEVIREEITDLPEDEITIIATGPLTSDKLAEKIHEFNGADGFYFY
DAAAPIIDANSINFDKVYKKSRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
MLFGPMKPVGLEYPEDYKGPRDGDFKTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMH
RNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAASGLVAGINAVKLFNDEEVVIFPKTTAIGSLPYYITHTDSK
HFQPMNVTFGIVEELEGPRIRDKKERYTKVAERALNSLTDIISKDNL
>Mature_447_residues
MKKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKQTPQHKTDKFAELVCSNSLRGAAITNAVGLLKEEMRRLDSVI
IKAAEVTQVPAGGALAVDREGFSDFVTREVSNHPLVEVIREEITDLPEDEITIIATGPLTSDKLAEKIHEFNGADGFYFY
DAAAPIIDANSINFDKVYKKSRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
MLFGPMKPVGLEYPEDYKGPRDGDFKTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMH
RNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAASGLVAGINAVKLFNDEEVVIFPKTTAIGSLPYYITHTDSK
HFQPMNVTFGIVEELEGPRIRDKKERYTKVAERALNSLTDIISKDNL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=42.2222222222222, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17534255, Length=72, Percent_Identity=45.8333333333333, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49992; Mature: 49992

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKQTPQHKTDKFAELVCSNSLRGA
CCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCH
AITNAVGLLKEEMRRLDSVIIKAAEVTQVPAGGALAVDREGFSDFVTREVSNHPLVEVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHH
EEITDLPEDEITIIATGPLTSDKLAEKIHEFNGADGFYFYDAAAPIIDANSINFDKVYKK
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHH
SRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
HCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCEE
MLFGPMKPVGLEYPEDYKGPRDGDFKTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKR
EEECCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHH
VFQMIPGLENAEFVRYGVMHRNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAA
HHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHH
SGLVAGINAVKLFNDEEVVIFPKTTAIGSLPYYITHTDSKHFQPMNVTFGIVEELEGPRI
HHHHHCCCEEEEECCCEEEEEECCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCC
RDKKERYTKVAERALNSLTDIISKDNL
CCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKQTPQHKTDKFAELVCSNSLRGA
CCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCH
AITNAVGLLKEEMRRLDSVIIKAAEVTQVPAGGALAVDREGFSDFVTREVSNHPLVEVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHH
EEITDLPEDEITIIATGPLTSDKLAEKIHEFNGADGFYFYDAAAPIIDANSINFDKVYKK
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHH
SRYDKGEADYINCPMTKEEFQAFQEALISAEEAPLNSFEDLKVFEGCMPIEEMAKRGYKT
HCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCEE
MLFGPMKPVGLEYPEDYKGPRDGDFKTPYAVVQLRQDNASASLYNIVGFQTHLKWGEQKR
EEECCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHH
VFQMIPGLENAEFVRYGVMHRNSYMDSPNLLKQTFQSRKQENLFFAGQMTGVEGYVESAA
HHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHH
SGLVAGINAVKLFNDEEVVIFPKTTAIGSLPYYITHTDSKHFQPMNVTFGIVEELEGPRI
HHHHHCCCEEEEECCCEEEEEECCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCC
RDKKERYTKVAERALNSLTDIISKDNL
CCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA