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The map label for this gene is topA

Identifier: 15673212

GI number: 15673212

Start: 1254565

End: 1256697

Strand: Direct

Name: topA

Synonym: L0298

Alternate gene names: 15673212

Gene position: 1254565-1256697 (Clockwise)

Preceding gene: 30024029

Following gene: 15673213

Centisome position: 53.03

GC content: 36.94

Gene sequence:

>2133_bases
ATGCCAACTTCAACTAAAAGTAAAACAAAAACAACTACAAAGAAAAAAACAACACGAAAAAGAGTCACTCCAGGTAAAAA
TCTGGTTATTGTAGAATCGCCAGCAAAAGCTAAAACAATTGAAAAATATTTAGGTCGAAACTACAAAGTTGTGGCTTCAG
TAGGTCATATTCGTGATTTGAAAAAATCAACCATGTCTGTTGATATAGAGAATGACTATGAACCTCAATATATTAATATT
CGTGGGAAAGCCCCATTAATCAACTCTCTCAAAAAAGAAGCAAAGGCTGCCAAAGCCGTCTATCTCGCAAGTGACCCGGA
CCGTGAAGGTGAAGCAATTTCTTGGCACTTAGCACATATCTTAAATCTTCCACTTGAAGAAAAAAATCGAGTGGTCTTTA
ATGAAATCACTAAAGATGCTGTTAAAAATGCCTTTAAGGAGCCGCGTCAAATTGATGTGGATCTAGTTGATGCCCAACAA
GCACGCCGAGTTCTTGACCGTTTGGTGGGTTATTCAATTAGTCCTATTCTATGGAAAAAAGTCAAAAAAGGACTTTCAGC
AGGTCGAGTTCAATCTATTGCCTTAAAACTAATTGTTGACCGCGAGAATGAAATTAATGCTTTTATTCCAGAAGAGTATT
GGTCAATTGATGGTGAATTTAAAAAAGGAACTAAAAAATTCAAAGCATCCTTCTGGGGAATTGACGGAAAAAAACATCCT
TTAAAAACCAATGAAGATGTCATCAAAGTCATGGAACGTTTAGACGGTCCTGATTTTAACGTAGATAAAGTTGAAAAGAA
AGAACGTCGCAGAAACGCTCCTCTTCCTTATACAACTAGCTCAATGCAACAAGACGCAGCTAATAAACTTAACTTTCGTA
CCCGTAAAACAATGATGGTTGCTCAACAACTTTATGAAGGTCTTACTTTAGGAAGCCAGGGCCATCAAGGTTTAATTACC
TATATGCGTACAGACTCAACACGTATTTCTCCTGTTGCACAAAGTGCAGCTCACAATTATATTGCTGATCAATTTGGAGA
AAAATATAGTAAACACGGTTCAAAAGTTAAAAATGCCTCTGGAGCCCAAGATGCCCACGAAGCAATTCGACCTTCAAATG
TCTTTAACACTCCAGACGCCATTGCTAAATATCTAGACAAAGATCAACTAAAACTTTACACACTCATCTGGAATCGTTTC
GTTGCCAGTCAAATGGTCGCAGCTATTTTCGATACTGTTAAAGTAAATCTGTCTCAAAATGGTGTTATCTTTATTGCTAA
TGGTTCACAAGTGAAATTTGACGGTTATCTGCGAATCTACAATGATTCTGATAAATCAAATATGCTTCCAGAGATGGAAG
AAAATGAAACTGTCAAAAAAGCGAATGTTAAACCAGAACAACATTTCACTCAACCACCAGCTAGATATTCCGAAGCAACA
CTCATCAAGACATTGGAAGAAAATGGAGTGGGACGTCCCTCAACCTATGCGCCTACACTAGAAACAATTCAAAAACGTTA
CTATGTGAGATTGTCAGCAAAACGTTTTGAACCTACAGAGCTTGGAGAAATTGTCAATAAGCTTATTGTTGAGTTCTTCC
CTAACATTGTAAATACTGAATTTACAGCTGAAATGGAAAAAGATCTTGATGAAGTTGAAGAAGGAAAACGTCAATGGATT
GAGGTTGTTGACCAATTTTACAAACCCTTTGCAAAAGAACTTGAAAATGCCGAAGAAGGTATGGAAAAAATTCAAATCAA
GGATGAACCTGCTGGCTTTGACTGTGATGTTTGTGGAAGTCCGATGGTAATTAAGTTGGGACGTTTTGGAAAATTTTACG
CTTGTAGCAACTTCCCAGATTGCCGAAACACCAAAGCAATTGTCAAAGAAATAGGCGTAAAATGTCCACTTTGCCATGAA
GGAAATATTATTGAACGCAAAACAAAGAAAAATAGAATTTTCTATGGTTGTGATCGTTACCCAGACTGTGAATTCACAAG
TTGGGACAAACCTATCGGACGTGATTGTCCTAAATCTGGACATTTTCTTGTTGAAAAGAAAGTTCGTGGTGGCGGTAAAC
AAGTGGTATGTTCAAATGATGAATGTGATTATCAAGAAGAAAAACAAAAATAA

Upstream 100 bases:

>100_bases
TAAACTATTATTTTACTTCCACATTCGATATATACCTCTATGAATTAAGATACAATAACTAAAAGATAGTGGATACCAAA
AAGAATAAATAGGAAAAATT

Downstream 100 bases:

>100_bases
AAGGCGCTTCCGCGCTTTTTTATTATATTAATCAATGGAAAAATTTACTAAATTTAGATATAATTGGGACTATGAAAAAA
ACACATATTAACGTTATAGG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MPTSTKSKTKTTTKKKTTRKRVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLKKSTMSVDIENDYEPQYINI
RGKAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHILNLPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQ
ARRVLDRLVGYSISPILWKKVKKGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHP
LKTNEDVIKVMERLDGPDFNVDKVEKKERRRNAPLPYTTSSMQQDAANKLNFRTRKTMMVAQQLYEGLTLGSQGHQGLIT
YMRTDSTRISPVAQSAAHNYIADQFGEKYSKHGSKVKNASGAQDAHEAIRPSNVFNTPDAIAKYLDKDQLKLYTLIWNRF
VASQMVAAIFDTVKVNLSQNGVIFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENETVKKANVKPEQHFTQPPARYSEAT
LIKTLEENGVGRPSTYAPTLETIQKRYYVRLSAKRFEPTELGEIVNKLIVEFFPNIVNTEFTAEMEKDLDEVEEGKRQWI
EVVDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGSPMVIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHE
GNIIERKTKKNRIFYGCDRYPDCEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK

Sequences:

>Translated_710_residues
MPTSTKSKTKTTTKKKTTRKRVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLKKSTMSVDIENDYEPQYINI
RGKAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHILNLPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQ
ARRVLDRLVGYSISPILWKKVKKGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHP
LKTNEDVIKVMERLDGPDFNVDKVEKKERRRNAPLPYTTSSMQQDAANKLNFRTRKTMMVAQQLYEGLTLGSQGHQGLIT
YMRTDSTRISPVAQSAAHNYIADQFGEKYSKHGSKVKNASGAQDAHEAIRPSNVFNTPDAIAKYLDKDQLKLYTLIWNRF
VASQMVAAIFDTVKVNLSQNGVIFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENETVKKANVKPEQHFTQPPARYSEAT
LIKTLEENGVGRPSTYAPTLETIQKRYYVRLSAKRFEPTELGEIVNKLIVEFFPNIVNTEFTAEMEKDLDEVEEGKRQWI
EVVDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGSPMVIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHE
GNIIERKTKKNRIFYGCDRYPDCEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK
>Mature_709_residues
PTSTKSKTKTTTKKKTTRKRVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLKKSTMSVDIENDYEPQYINIR
GKAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHILNLPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQA
RRVLDRLVGYSISPILWKKVKKGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHPL
KTNEDVIKVMERLDGPDFNVDKVEKKERRRNAPLPYTTSSMQQDAANKLNFRTRKTMMVAQQLYEGLTLGSQGHQGLITY
MRTDSTRISPVAQSAAHNYIADQFGEKYSKHGSKVKNASGAQDAHEAIRPSNVFNTPDAIAKYLDKDQLKLYTLIWNRFV
ASQMVAAIFDTVKVNLSQNGVIFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENETVKKANVKPEQHFTQPPARYSEATL
IKTLEENGVGRPSTYAPTLETIQKRYYVRLSAKRFEPTELGEIVNKLIVEFFPNIVNTEFTAEMEKDLDEVEEGKRQWIE
VVDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGSPMVIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHEG
NIIERKTKKNRIFYGCDRYPDCEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI10835218, Length=617, Percent_Identity=26.904376012966, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI4507635, Length=538, Percent_Identity=23.6059479553903, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787529, Length=752, Percent_Identity=37.6329787234042, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI48994931, Length=111, Percent_Identity=38.7387387387387, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788061, Length=543, Percent_Identity=22.6519337016575, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17555378, Length=510, Percent_Identity=24.9019607843137, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=653, Percent_Identity=23.8897396630934, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=556, Percent_Identity=23.9208633093525, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24585251, Length=672, Percent_Identity=22.6190476190476, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP1_LACLA (Q9CG80)

Other databases:

- EMBL:   AE005176
- PIR:   F86778
- RefSeq:   NP_267386.1
- ProteinModelPortal:   Q9CG80
- SMR:   Q9CG80
- GeneID:   1114880
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0298
- NMPDR:   fig|272623.1.peg.1266
- HOGENOM:   HBG512935
- OMA:   EEYWSID
- ProtClustDB:   PRK05582
- BioCyc:   LLAC272623:L0298-MONOMER
- BRENDA:   5.99.1.2
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01051

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 80742; Mature: 80611

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 321-321

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSTKSKTKTTTKKKTTRKRVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDL
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCEEEEEEHHHHHHH
KKSTMSVDIENDYEPQYINIRGKAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHI
HHHHEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEHHHHH
LNLPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQARRVLDRLVGYSISPILWKK
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECEEEECHHHHHHHHHHHHCCCCCHHHHHH
VKKGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHP
HHHCCCCCHHHHEEEEEEECCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
LKTNEDVIKVMERLDGPDFNVDKVEKKERRRNAPLPYTTSSMQQDAANKLNFRTRKTMMV
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHH
AQQLYEGLTLGSQGHQGLITYMRTDSTRISPVAQSAAHNYIADQFGEKYSKHGSKVKNAS
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GAQDAHEAIRPSNVFNTPDAIAKYLDKDQLKLYTLIWNRFVASQMVAAIFDTVKVNLSQN
CCHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
GVIFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENETVKKANVKPEQHFTQPPARYSEAT
CEEEEECCCEEEECCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHCCCCCCHHHHHH
LIKTLEENGVGRPSTYAPTLETIQKRYYVRLSAKRFEPTELGEIVNKLIVEFFPNIVNTE
HHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCH
FTAEMEKDLDEVEEGKRQWIEVVDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCC
PMVIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHEGNIIERKTKKNRIFYGCDRY
CCEEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEHHHHCCCCEEECCCCC
PDCEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHCC
>Mature Secondary Structure 
PTSTKSKTKTTTKKKTTRKRVTPGKNLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDL
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCEEEEEEHHHHHHH
KKSTMSVDIENDYEPQYINIRGKAPLINSLKKEAKAAKAVYLASDPDREGEAISWHLAHI
HHHHEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEHHHHH
LNLPLEEKNRVVFNEITKDAVKNAFKEPRQIDVDLVDAQQARRVLDRLVGYSISPILWKK
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECEEEECHHHHHHHHHHHHCCCCCHHHHHH
VKKGLSAGRVQSIALKLIVDRENEINAFIPEEYWSIDGEFKKGTKKFKASFWGIDGKKHP
HHHCCCCCHHHHEEEEEEECCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
LKTNEDVIKVMERLDGPDFNVDKVEKKERRRNAPLPYTTSSMQQDAANKLNFRTRKTMMV
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHH
AQQLYEGLTLGSQGHQGLITYMRTDSTRISPVAQSAAHNYIADQFGEKYSKHGSKVKNAS
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GAQDAHEAIRPSNVFNTPDAIAKYLDKDQLKLYTLIWNRFVASQMVAAIFDTVKVNLSQN
CCHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
GVIFIANGSQVKFDGYLRIYNDSDKSNMLPEMEENETVKKANVKPEQHFTQPPARYSEAT
CEEEEECCCEEEECCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHCCCCCCHHHHHH
LIKTLEENGVGRPSTYAPTLETIQKRYYVRLSAKRFEPTELGEIVNKLIVEFFPNIVNTE
HHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCH
FTAEMEKDLDEVEEGKRQWIEVVDQFYKPFAKELENAEEGMEKIQIKDEPAGFDCDVCGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCC
PMVIKLGRFGKFYACSNFPDCRNTKAIVKEIGVKCPLCHEGNIIERKTKKNRIFYGCDRY
CCEEEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEHHHHCCCCEEECCCCC
PDCEFTSWDKPIGRDCPKSGHFLVEKKVRGGGKQVVCSNDECDYQEEKQK
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471