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The map label for this gene is ilvA

Identifier: 15673209

GI number: 15673209

Start: 1250814

End: 1252064

Strand: Direct

Name: ilvA

Synonym: L0081

Alternate gene names: 15673209

Gene position: 1250814-1252064 (Clockwise)

Preceding gene: 15673208

Following gene: 15673210

Centisome position: 52.88

GC content: 36.05

Gene sequence:

>1251_bases
ATGATAAGTGCCAAAGAGGTTGAAGATGCCTATGATTTGTTAAAAGCAGTTGTCACTAAAACACCTTTACAATTAGACCC
CTACCTTTCCAATAAATATCAAGCAAATATTTACTTAAAAGAAGAAAATTTACAGAAAGTTCGTTCTTTTAAATTACGAG
GAGCTTATTATTCTATCAGTAAATTATCTGATGAGCAACGCTCTAAAGGAGTGGTTTGTGCCTCCGCAGGTAATCACGCA
CAAGGAGTTGCTTTTGCTGCAAATCAATTAAATATTTCTGCGACAATTTTCATGCCCGTTACTACACCTAACCAAAAAAT
TTCACAAGTTAAATTTTTTGGCGAAAGTCATGTAACAATTCGTTTAATTGGTGATACTTTTGATGAATCAGCCAGAGCAG
CAAAAGCTTTTTCTCAAGATAATGACAAACCATTTATAGACCCTTTTGATGATGAAAATGTAATTGCTGGTCAAGGGACA
GTGGCTTTAGAAATTTTTGCGCAAGCTAAAAAACAAGGAATAAGTTTAGATAAGATTTTTGTACAGATTGGTGGAGGTGG
TTTAATTGCAGGAATTACGGCCTACAGTAAGGAGCGCTATCCCCAAACTGAAATTATTGGAGTTGAAGCAAAAGGGGCAA
CAAGTATGAAAGCTGCCTACTCTGCTGGTCAGCCCGTCACCTTGGAACACATTGATAAATTTGCTGACGGAATTGCGGTT
GCGACTGTTGGTCAGAAAACTTACCAACTTATTAATGACAAAGTGAAACAATTGCTTGCGGTTGATGAAGGTTTAATTTC
TCAAACCATACTCGAATTGTATTCAAAATTAGGAATTGTCGCCGAACCAGCAGGTGCAACATCTGTTGCCGCACTTGAAC
TTATTAAAGATGAAATCAAGGGTAAAAATATTGTCTGTATCATCAGCGGCGGAAATAATGATATTAGTCGAATGCAAGAA
ATTGAAGAAAGAGCTTTGGTTTATGAAGGTCTAAAACATTATTTTGTCATTAACTTTCCTCAAAGACCAGGAGCCTTAAG
AACTTTTGTCAGTGATATTTTAGGGCCAAATGATGATATTACCCGATTTGAGTACATCAAAAGGGCTGATAAAGGTAAAG
GGCCTTGTCTTGTTGGGATTTTACTTTCAGATGCTAGTGATTATGATTCATTGATTGATCGGATTGAAAGATTTGATAAT
CGTTATGTTAACTTACATGGAAATGATAGTTTATACGAACTTTTGGTCTAA

Upstream 100 bases:

>100_bases
TTCACACAATCTGGTGATGACGATGCCTTTAAAATCTATCAGTAATTTCTCTTATTGATTGAACAAAAACATAAAAGCAT
TTTATGGAGGAATGACATAA

Downstream 100 bases:

>100_bases
CTAACCAATTGGTTTGAGCCATTTTCTAGTTTCAATTCTCTTTAAATCACTAGAAATTTGATATAATTTAATTAATAAAT
TATTTAAATTTAATGAGATA

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MISAKEVEDAYDLLKAVVTKTPLQLDPYLSNKYQANIYLKEENLQKVRSFKLRGAYYSISKLSDEQRSKGVVCASAGNHA
QGVAFAANQLNISATIFMPVTTPNQKISQVKFFGESHVTIRLIGDTFDESARAAKAFSQDNDKPFIDPFDDENVIAGQGT
VALEIFAQAKKQGISLDKIFVQIGGGGLIAGITAYSKERYPQTEIIGVEAKGATSMKAAYSAGQPVTLEHIDKFADGIAV
ATVGQKTYQLINDKVKQLLAVDEGLISQTILELYSKLGIVAEPAGATSVAALELIKDEIKGKNIVCIISGGNNDISRMQE
IEERALVYEGLKHYFVINFPQRPGALRTFVSDILGPNDDITRFEYIKRADKGKGPCLVGILLSDASDYDSLIDRIERFDN
RYVNLHGNDSLYELLV

Sequences:

>Translated_416_residues
MISAKEVEDAYDLLKAVVTKTPLQLDPYLSNKYQANIYLKEENLQKVRSFKLRGAYYSISKLSDEQRSKGVVCASAGNHA
QGVAFAANQLNISATIFMPVTTPNQKISQVKFFGESHVTIRLIGDTFDESARAAKAFSQDNDKPFIDPFDDENVIAGQGT
VALEIFAQAKKQGISLDKIFVQIGGGGLIAGITAYSKERYPQTEIIGVEAKGATSMKAAYSAGQPVTLEHIDKFADGIAV
ATVGQKTYQLINDKVKQLLAVDEGLISQTILELYSKLGIVAEPAGATSVAALELIKDEIKGKNIVCIISGGNNDISRMQE
IEERALVYEGLKHYFVINFPQRPGALRTFVSDILGPNDDITRFEYIKRADKGKGPCLVGILLSDASDYDSLIDRIERFDN
RYVNLHGNDSLYELLV
>Mature_416_residues
MISAKEVEDAYDLLKAVVTKTPLQLDPYLSNKYQANIYLKEENLQKVRSFKLRGAYYSISKLSDEQRSKGVVCASAGNHA
QGVAFAANQLNISATIFMPVTTPNQKISQVKFFGESHVTIRLIGDTFDESARAAKAFSQDNDKPFIDPFDDENVIAGQGT
VALEIFAQAKKQGISLDKIFVQIGGGGLIAGITAYSKERYPQTEIIGVEAKGATSMKAAYSAGQPVTLEHIDKFADGIAV
ATVGQKTYQLINDKVKQLLAVDEGLISQTILELYSKLGIVAEPAGATSVAALELIKDEIKGKNIVCIISGGNNDISRMQE
IEERALVYEGLKHYFVINFPQRPGALRTFVSDILGPNDDITRFEYIKRADKGKGPCLVGILLSDASDYDSLIDRIERFDN
RYVNLHGNDSLYELLV

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family

Homologues:

Organism=Homo sapiens, GI11345492, Length=325, Percent_Identity=30.1538461538462, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33469958, Length=319, Percent_Identity=28.2131661442006, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI19923959, Length=310, Percent_Identity=27.4193548387097, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790207, Length=365, Percent_Identity=37.2602739726027, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789505, Length=322, Percent_Identity=35.4037267080745, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71991565, Length=341, Percent_Identity=35.4838709677419, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17537387, Length=365, Percent_Identity=31.2328767123288, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17508781, Length=318, Percent_Identity=26.7295597484277, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320930, Length=355, Percent_Identity=38.8732394366197, Blast_Score=206, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6322631, Length=312, Percent_Identity=35.5769230769231, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319788, Length=295, Percent_Identity=28.4745762711864, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322024, Length=188, Percent_Identity=26.5957446808511, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355833, Length=351, Percent_Identity=31.0541310541311, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24645328, Length=255, Percent_Identity=27.843137254902, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): THD1_LACLA (Q02145)

Other databases:

- EMBL:   U92974
- EMBL:   AE005176
- PIR:   C86778
- PIR:   S35141
- RefSeq:   NP_267383.1
- ProteinModelPortal:   Q02145
- SMR:   Q02145
- GeneID:   1114876
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0081
- NMPDR:   fig|272623.1.peg.1262
- HOGENOM:   HBG714501
- OMA:   DILMAHN
- ProtClustDB:   PRK08639
- BioCyc:   LLAC272623:L0081-MONOMER
- BRENDA:   4.3.1.19
- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820
- TIGRFAMs:   TIGR02079

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.3.1.19

Molecular weight: Translated: 45577; Mature: 45577

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAKEVEDAYDLLKAVVTKTPLQLDPYLSNKYQANIYLKEENLQKVRSFKLRGAYYSIS
CCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEHHHHHHHHHHHHCCCEEEHH
KLSDEQRSKGVVCASAGNHAQGVAFAANQLNISATIFMPVTTPNQKISQVKFFGESHVTI
HHCCHHHHCCEEEECCCCCCCCEEEEECEEEEEEEEEEEECCCCHHHHHEEEECCCCEEE
RLIGDTFDESARAAKAFSQDNDKPFIDPFDDENVIAGQGTVALEIFAQAKKQGISLDKIF
EEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEHHHHHHHHHHCCCEEEEE
VQIGGGGLIAGITAYSKERYPQTEIIGVEAKGATSMKAAYSAGQPVTLEHIDKFADGIAV
EEECCCEEEEEEEHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCEEHHHHHHHHCCEEE
ATVGQKTYQLINDKVKQLLAVDEGLISQTILELYSKLGIVAEPAGATSVAALELIKDEIK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHC
GKNIVCIISGGNNDISRMQEIEERALVYEGLKHYFVINFPQRPGALRTFVSDILGPNDDI
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCH
TRFEYIKRADKGKGPCLVGILLSDASDYDSLIDRIERFDNRYVNLHGNDSLYELLV
HHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHCC
>Mature Secondary Structure
MISAKEVEDAYDLLKAVVTKTPLQLDPYLSNKYQANIYLKEENLQKVRSFKLRGAYYSIS
CCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEHHHHHHHHHHHHCCCEEEHH
KLSDEQRSKGVVCASAGNHAQGVAFAANQLNISATIFMPVTTPNQKISQVKFFGESHVTI
HHCCHHHHCCEEEECCCCCCCCEEEEECEEEEEEEEEEEECCCCHHHHHEEEECCCCEEE
RLIGDTFDESARAAKAFSQDNDKPFIDPFDDENVIAGQGTVALEIFAQAKKQGISLDKIF
EEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEHHHHHHHHHHCCCEEEEE
VQIGGGGLIAGITAYSKERYPQTEIIGVEAKGATSMKAAYSAGQPVTLEHIDKFADGIAV
EEECCCEEEEEEEHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCEEHHHHHHHHCCEEE
ATVGQKTYQLINDKVKQLLAVDEGLISQTILELYSKLGIVAEPAGATSVAALELIKDEIK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHC
GKNIVCIISGGNNDISRMQEIEERALVYEGLKHYFVINFPQRPGALRTFVSDILGPNDDI
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCH
TRFEYIKRADKGKGPCLVGILLSDASDYDSLIDRIERFDNRYVNLHGNDSLYELLV
HHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471