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The map label for this gene is clsB

Identifier: 15673156

GI number: 15673156

Start: 1187132

End: 1188595

Strand: Direct

Name: clsB

Synonym: L186875

Alternate gene names: 15673156

Gene position: 1187132-1188595 (Clockwise)

Preceding gene: 15673155

Following gene: 15673158

Centisome position: 50.18

GC content: 34.97

Gene sequence:

>1464_bases
ATGGAGAAATATTTTATGTCACTTGCGACAATTTATAGTTTGATGGGAACACTAATAACGATTAACACATTGCTTGCCCT
TGTGACAATTTTTCGGAAACCTAGGTCTATTGCCAGTATTTTTGCGTGGTCTATGTTCTTGTTCTTTTTACCAGGGGTTG
GTTTCCTTGGCTATCTTTTTTTAGGAAGAGGGCTTGATCGAACAACAACAAATCGTTTTGAAGAAGAAAATAAATATAAT
CTGTCTATTCTTGCTCAAAGGGTGCGTGAAAATAATGAAAAATTTGAGCCAAAAGAAATGGCACCCCATGAAAGATTACT
GAAAAGGTATTTTAATAATATGGAACGTACACCACTTTGTCGTGGGAATAAAGTGAATTTTTATCTGAATGGAGAAGATA
AATTTTCAGCCTTGTTTGATGACATAAAGAATGCTAAAGATAATATTCATGTAGAATACTATGCATTCTTTAATGACAAA
ATTGGAACTGCTTTTCGCGATAATCTAATTGAAAAAGCTAAAGAAGGGGTGGAAGTTAGAGTAGTTTACGATCCATGGGG
AGGAAAAACGAGAAAGGATTTCTTTAAACCTTTGGAAGAGGCAGGAGGTAAGGTCACAGCCTTTATCACATCGCGTAATA
CACTAACAAAAACCCGACTTAACTATCATTTACATCGTAAAATTGTAGTGATAGATGGTCAAATTGGTTGGACTGGCGGA
TTTAATGTTGGTGATCAATATATTTATAACAGTAAAAAGTTTGGCTTCTGGCGCGATACACACGGTAGAATTGTTGGTAC
AGCAGCTTTTGGTCTTCAAGAAACATTTATTCGAGATTGGAATGTTTCAGTCAGAGATCCTAAAGATAAGCTTGAAAGAG
AAGATTCATATTTTGTGGTACCGGAAGAAGAAGGGAACATTGATATTCAGATTGTCGCTAACGGACCAAAGAGTGATAAT
AAAACCTTAAGAACAGGTTTTATTAAAATGATTATGGATGCGGAAGACTATATTTGGCTTCAATCGCCTTACTTGATACC
TGATGACTCTATGATAACAGCGCTTGTAGCAGCGGCGAATTCTGGTGTTGATGTAAGAATTATGATTCCCAATATGCCAG
ATCATCCGTTTATTTTTAGAGCAACACAATATTACGCAAATTATCTACATAAACACGGTGTGAAAATATACAATTATACA
AATGGCTTCATACACTCTAAAACTCTTGTAATGGATGGAAAATTAGGAGTATTTGGTACAACTAATCAAGATATTCGCAG
TTATGAATTAAATTTCGAGATAAGTGCTTTTTGTTATGATGAAACTGTAGCAAAAGAGATGAGCAGGACCTTTGAGGGAG
ACTTAAGAAATTCAAAGCTACTTACTGATGAAGAAATTGCCCAACAATCTATCTGGCTTAAAATCAAGCAAAACTTTTCG
AGATTACTTAGTCCGATATTATAA

Upstream 100 bases:

>100_bases
ATACTTATAAAAATAACATTAAATTTTGCTTAGATAAAATACATAAAGTATCCAATAAATTTTGATATAATTAGATAAAT
AATATTATTTTATGGAGATT

Downstream 100 bases:

>100_bases
ATGGTCTTGTATATAGGGCTTAATAAGGATGAATTGACTCCTTTAAAATACATTAATATTGAAAAATAAAAATCACTTGA
AAATTTTAGTGATTTTTATT

Product: cardiolipin synthase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MEKYFMSLATIYSLMGTLITINTLLALVTIFRKPRSIASIFAWSMFLFFLPGVGFLGYLFLGRGLDRTTTNRFEEENKYN
LSILAQRVRENNEKFEPKEMAPHERLLKRYFNNMERTPLCRGNKVNFYLNGEDKFSALFDDIKNAKDNIHVEYYAFFNDK
IGTAFRDNLIEKAKEGVEVRVVYDPWGGKTRKDFFKPLEEAGGKVTAFITSRNTLTKTRLNYHLHRKIVVIDGQIGWTGG
FNVGDQYIYNSKKFGFWRDTHGRIVGTAAFGLQETFIRDWNVSVRDPKDKLEREDSYFVVPEEEGNIDIQIVANGPKSDN
KTLRTGFIKMIMDAEDYIWLQSPYLIPDDSMITALVAAANSGVDVRIMIPNMPDHPFIFRATQYYANYLHKHGVKIYNYT
NGFIHSKTLVMDGKLGVFGTTNQDIRSYELNFEISAFCYDETVAKEMSRTFEGDLRNSKLLTDEEIAQQSIWLKIKQNFS
RLLSPIL

Sequences:

>Translated_487_residues
MEKYFMSLATIYSLMGTLITINTLLALVTIFRKPRSIASIFAWSMFLFFLPGVGFLGYLFLGRGLDRTTTNRFEEENKYN
LSILAQRVRENNEKFEPKEMAPHERLLKRYFNNMERTPLCRGNKVNFYLNGEDKFSALFDDIKNAKDNIHVEYYAFFNDK
IGTAFRDNLIEKAKEGVEVRVVYDPWGGKTRKDFFKPLEEAGGKVTAFITSRNTLTKTRLNYHLHRKIVVIDGQIGWTGG
FNVGDQYIYNSKKFGFWRDTHGRIVGTAAFGLQETFIRDWNVSVRDPKDKLEREDSYFVVPEEEGNIDIQIVANGPKSDN
KTLRTGFIKMIMDAEDYIWLQSPYLIPDDSMITALVAAANSGVDVRIMIPNMPDHPFIFRATQYYANYLHKHGVKIYNYT
NGFIHSKTLVMDGKLGVFGTTNQDIRSYELNFEISAFCYDETVAKEMSRTFEGDLRNSKLLTDEEIAQQSIWLKIKQNFS
RLLSPIL
>Mature_487_residues
MEKYFMSLATIYSLMGTLITINTLLALVTIFRKPRSIASIFAWSMFLFFLPGVGFLGYLFLGRGLDRTTTNRFEEENKYN
LSILAQRVRENNEKFEPKEMAPHERLLKRYFNNMERTPLCRGNKVNFYLNGEDKFSALFDDIKNAKDNIHVEYYAFFNDK
IGTAFRDNLIEKAKEGVEVRVVYDPWGGKTRKDFFKPLEEAGGKVTAFITSRNTLTKTRLNYHLHRKIVVIDGQIGWTGG
FNVGDQYIYNSKKFGFWRDTHGRIVGTAAFGLQETFIRDWNVSVRDPKDKLEREDSYFVVPEEEGNIDIQIVANGPKSDN
KTLRTGFIKMIMDAEDYIWLQSPYLIPDDSMITALVAAANSGVDVRIMIPNMPDHPFIFRATQYYANYLHKHGVKIYNYT
NGFIHSKTLVMDGKLGVFGTTNQDIRSYELNFEISAFCYDETVAKEMSRTFEGDLRNSKLLTDEEIAQQSIWLKIKQNFS
RLLSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=500, Percent_Identity=25.8, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787007, Length=363, Percent_Identity=25.068870523416, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 56323; Mature: 56323

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYFMSLATIYSLMGTLITINTLLALVTIFRKPRSIASIFAWSMFLFFLPGVGFLGYLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
LGRGLDRTTTNRFEEENKYNLSILAQRVRENNEKFEPKEMAPHERLLKRYFNNMERTPLC
HCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHCCCCCCCCC
RGNKVNFYLNGEDKFSALFDDIKNAKDNIHVEYYAFFNDKIGTAFRDNLIEKAKEGVEVR
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEE
VVYDPWGGKTRKDFFKPLEEAGGKVTAFITSRNTLTKTRLNYHLHRKIVVIDGQIGWTGG
EEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEHHHHCEEEEEEEEEEECCCCCCCC
FNVGDQYIYNSKKFGFWRDTHGRIVGTAAFGLQETFIRDWNVSVRDPKDKLEREDSYFVV
CCCCCHHEECCCCCCEEECCCCCEEEEEHHHHHHHHHHCCCCEECCCHHHHCCCCCEEEE
PEEEGNIDIQIVANGPKSDNKTLRTGFIKMIMDAEDYIWLQSPYLIPDDSMITALVAAAN
ECCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCEECCCCHHHHHHHHHCC
SGVDVRIMIPNMPDHPFIFRATQYYANYLHKHGVKIYNYTNGFIHSKTLVMDGKLGVFGT
CCCEEEEEECCCCCCCEEEEHHHHHHHHHHHCCCEEEEECCCEEEEEEEEECCCEEEEEC
TNQDIRSYELNFEISAFCYDETVAKEMSRTFEGDLRNSKLLTDEEIAQQSIWLKIKQNFS
CCCCCEEEEEEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLLSPIL
HHHHCCC
>Mature Secondary Structure
MEKYFMSLATIYSLMGTLITINTLLALVTIFRKPRSIASIFAWSMFLFFLPGVGFLGYLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
LGRGLDRTTTNRFEEENKYNLSILAQRVRENNEKFEPKEMAPHERLLKRYFNNMERTPLC
HCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHCCCCCCCCC
RGNKVNFYLNGEDKFSALFDDIKNAKDNIHVEYYAFFNDKIGTAFRDNLIEKAKEGVEVR
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEE
VVYDPWGGKTRKDFFKPLEEAGGKVTAFITSRNTLTKTRLNYHLHRKIVVIDGQIGWTGG
EEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEHHHHCEEEEEEEEEEECCCCCCCC
FNVGDQYIYNSKKFGFWRDTHGRIVGTAAFGLQETFIRDWNVSVRDPKDKLEREDSYFVV
CCCCCHHEECCCCCCEEECCCCCEEEEEHHHHHHHHHHCCCCEECCCHHHHCCCCCEEEE
PEEEGNIDIQIVANGPKSDNKTLRTGFIKMIMDAEDYIWLQSPYLIPDDSMITALVAAAN
ECCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCEECCCCHHHHHHHHHCC
SGVDVRIMIPNMPDHPFIFRATQYYANYLHKHGVKIYNYTNGFIHSKTLVMDGKLGVFGT
CCCEEEEEECCCCCCCEEEEHHHHHHHHHHHCCCEEEEECCCEEEEEEEEECCCEEEEEC
TNQDIRSYELNFEISAFCYDETVAKEMSRTFEGDLRNSKLLTDEEIAQQSIWLKIKQNFS
CCCCCEEEEEEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLLSPIL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]