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The map label for this gene is prfB

Identifier: 15672953

GI number: 15672953

Start: 998162

End: 999259

Strand: Direct

Name: prfB

Synonym: L0374

Alternate gene names: 15672953

Gene position: 998162-999259 (Clockwise)

Preceding gene: 15672950

Following gene: 15672954

Centisome position: 42.2

GC content: 40.26

Gene sequence:

>1098_bases
ATGGAATTATCAGAAATTAGAAATTTACTTGAAGGATATGCTGAGAAGATTAATGGCTTTCGGGAATCATTAGATCTTGA
GCGTTTGGAAGAAGAAATAGCTCTTTTAGAAAACGATATGGCTCAACCTGAATTTTGGAATGATCAAGCAGCGGCTCAAA
AAGTGATTGATGAATCAAATGCTTTGAAAGCCAAGTATGACAATTATCAAGCGATGAATAATATGCTTGAGGAAGCGCAA
ACGATGCTTGAGATGCTTCAAGAAGAAGCTGACGAAGAGATGCAAGCTGAGTTAGAGGAAATGACAATTGCGCTCGGTCA
AAAAATTGAATCTTATGAGCTTGAAATTATGCTCAATCAACCTTATGACCATATGAATGCTGTTCTTGAAATTCACCCAG
GTTCTGGGGGAACAGAGTCGCAGGACTGGGGCTCAATGTTGATGCGGATGTACACTCGTTGGGGCGAAGCACATGGATTT
AAGGTTGAAATTCTCGACTATCAAGACGGAGATGTTGCCGGATTGAAATCAGTTGCCCTTCGATTTGTTGGACGAAATGC
TTATGGTTTTTTGAGAGGAGAAAAAGGAGTACATCGTTTAGTACGTATTTCACCTTTTGATTCAGCCAATCGTCGTCATA
CTTCATTTACCTCAGTTGATGTGATGCCTGAGTTGGACGATTCTATTGAAGTAGATGTTCGTGATGCCGATGTTAAAATG
GATACTTTCCGTTCGGGTGGAGCTGGTGGACAAAATGTCAATAAAGTCTCTACTGGTGTGCGTTTGACTCACATCCCAAC
GGGAATTGTTGTGCAATCTACAATGGATAGAACGCAATATGGTAACCGGGATAAAGCGATGGCTATGCTGAAATCGAAAC
TCTATCAATTAGAAATGGATAAAAAGCAAGCTGAAGTTGATGAACTTCGTGGCGACCAATCAGAAATTTCTTGGGGAAGT
CAAATTCGTTCATACGTATTCATGCCTTATCAATTAGTTAAAGATACGCGTACTGGTTATGAAACTGGACAAATTTCAAA
TGTGATGGACGGAGAATTGGACGGGTTTATAAATGCCTATCTCCGTTGGAACTTGTAG

Upstream 100 bases:

>100_bases
GTGATAGAAGTTCTAACTAATAAATAAATTTTTGATGAATCCTTATCTATTGAATAGCTTACTTTAGGCTTATAAGTGGT
GAATATGGTATAATGCTACT

Downstream 100 bases:

>100_bases
ATTATAAATTAAAGTTTCGTATGTTTGAAATGTTTACGAAATATTAAAGTTATTAATGAAAAGGTTATAAAATAAAAGTG
CCACCTAAAGCACTTTTATT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MELSEIRNLLEGYAEKINGFRESLDLERLEEEIALLENDMAQPEFWNDQAAAQKVIDESNALKAKYDNYQAMNNMLEEAQ
TMLEMLQEEADEEMQAELEEMTIALGQKIESYELEIMLNQPYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGF
KVEILDYQDGDVAGLKSVALRFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVDVRDADVKM
DTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMDKKQAEVDELRGDQSEISWGS
QIRSYVFMPYQLVKDTRTGYETGQISNVMDGELDGFINAYLRWNL

Sequences:

>Translated_365_residues
MELSEIRNLLEGYAEKINGFRESLDLERLEEEIALLENDMAQPEFWNDQAAAQKVIDESNALKAKYDNYQAMNNMLEEAQ
TMLEMLQEEADEEMQAELEEMTIALGQKIESYELEIMLNQPYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGF
KVEILDYQDGDVAGLKSVALRFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVDVRDADVKM
DTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMDKKQAEVDELRGDQSEISWGS
QIRSYVFMPYQLVKDTRTGYETGQISNVMDGELDGFINAYLRWNL
>Mature_365_residues
MELSEIRNLLEGYAEKINGFRESLDLERLEEEIALLENDMAQPEFWNDQAAAQKVIDESNALKAKYDNYQAMNNMLEEAQ
TMLEMLQEEADEEMQAELEEMTIALGQKIESYELEIMLNQPYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGF
KVEILDYQDGDVAGLKSVALRFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVDVRDADVKM
DTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMDKKQAEVDELRGDQSEISWGS
QIRSYVFMPYQLVKDTRTGYETGQISNVMDGELDGFINAYLRWNL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI34577120, Length=307, Percent_Identity=32.2475570032573, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI166795303, Length=313, Percent_Identity=29.7124600638978, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=30.3738317757009, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI2367172, Length=365, Percent_Identity=45.4794520547945, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1787462, Length=303, Percent_Identity=36.3036303630363, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=252, Percent_Identity=30.952380952381, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321295, Length=315, Percent_Identity=29.2063492063492, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI19921226, Length=291, Percent_Identity=29.553264604811, Blast_Score=134, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_LACLA (Q9CGX1)

Other databases:

- EMBL:   AE005176
- PIR:   C86746
- RefSeq:   NP_267127.1
- ProteinModelPortal:   Q9CGX1
- SMR:   Q9CGX1
- GeneID:   1114602
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0374
- NMPDR:   fig|272623.1.peg.994
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- BioCyc:   LLAC272623:L0374-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41570; Mature: 41570

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSEIRNLLEGYAEKINGFRESLDLERLEEEIALLENDMAQPEFWNDQAAAQKVIDESN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
ALKAKYDNYQAMNNMLEEAQTMLEMLQEEADEEMQAELEEMTIALGQKIESYELEIMLNQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
PYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGFKVEILDYQDGDVAGLKSVAL
CHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
RFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVDVRDADVKM
HHHCCCCCEEECCCCCHHHHEEECCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEEH
DTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMD
HHCCCCCCCCCCCHHHHCCEEEEECCCCEEEECHHHHHHCCCHHHHHHHHHHHHHHHHCC
KKQAEVDELRGDQSEISWGSQIRSYVFMPYQLVKDTRTGYETGQISNVMDGELDGFINAY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHH
LRWNL
EEECC
>Mature Secondary Structure
MELSEIRNLLEGYAEKINGFRESLDLERLEEEIALLENDMAQPEFWNDQAAAQKVIDESN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
ALKAKYDNYQAMNNMLEEAQTMLEMLQEEADEEMQAELEEMTIALGQKIESYELEIMLNQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
PYDHMNAVLEIHPGSGGTESQDWGSMLMRMYTRWGEAHGFKVEILDYQDGDVAGLKSVAL
CHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
RFVGRNAYGFLRGEKGVHRLVRISPFDSANRRHTSFTSVDVMPELDDSIEVDVRDADVKM
HHHCCCCCEEECCCCCHHHHEEECCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEEH
DTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTMDRTQYGNRDKAMAMLKSKLYQLEMD
HHCCCCCCCCCCCHHHHCCEEEEECCCCEEEECHHHHHHCCCHHHHHHHHHHHHHHHHCC
KKQAEVDELRGDQSEISWGSQIRSYVFMPYQLVKDTRTGYETGQISNVMDGELDGFINAY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHH
LRWNL
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471