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The map label for this gene is mleR

Identifier: 15672854

GI number: 15672854

Start: 895741

End: 896616

Strand: Direct

Name: mleR

Synonym: L0148

Alternate gene names: 15672854

Gene position: 895741-896616 (Clockwise)

Preceding gene: 15672853

Following gene: 15672855

Centisome position: 37.87

GC content: 29.91

Gene sequence:

>876_bases
ATGAGTTTAAATTTAAGAGATCTTGAATATTTTTATCAATTGTCAAAATTGCGTTCGTTCACCAATGTTGCCAAACATTT
CAGGGTTAGTCAACCCACTATTTCTTACGCAATCAAACGTTTAGAAACCTACTATGACTGTGATTTATTTTATAAAGATT
CCTCGCATCAGGTAGTTGATTTAACTCCGGAGGGCGAACTTTTGGCAATGAGAACCAATGAAATTCTCTCTGAACTAAAT
CACACGAAAAAAGCTATTCATCGTTCGAGTATGAAAGATTTTAAAGTTGGTTTTCCGCCAATTATAAGTTCATATCTTCT
ATCAAAGCAAGATAATTTGAACTTTTTTAATGCCTTACATATGATTTATGGTGGTTCTAATGAACTCTTGAGCCTACTTT
TAAATGAAGAAATAGATTCGAGCCTTTTAGGAAGTCTAGAAAAGATTAAGCATCCCCAATTAGAAATAGAAGAACTCTTT
CAAAAAGAATTTTTTATCATTATGTCGGATAGTCATCCACTAGCCAATGAAAAAGAATTAGGATTTAAAGACTTACTTAA
TGAAGACTTTATCTTATTGGGAGAGCATAATATCCACTTTAATGCCTTTAATCGTTTAAATGAAAAATATAATCAATCAG
CTAATGTAGTTTTGAAATTAGATGATGCTCATACCATAAAAGAACTTGTCAGGAAAAATTTAGGGATCAGTCTTATGGCA
GATATCCGCTTATCAGAAGACTTTAAAAATCTAGTTAAAGTACCATTTATTGAAGAAGATAAACAATTCTTTCATATTAA
TTATGCCTATCAAAAAAATTCTATTCTCTCTGAAAGTGAAAAAAACTTTTTAGAAATATTGAAAACATTAAAATAA

Upstream 100 bases:

>100_bases
CTCAAGGTTTATGGACAATTAAACTAGAGTTGCTCAATAATACTTATGGCACTAAGAATAATTACAAAATTATGTTAAAA
TAGCTTCTAGGAGTATAGCC

Downstream 100 bases:

>100_bases
ATATTGCATAAGAGCATCAATGATTTGGTGCTTTTTTGATAGTAATTATTTACAGTAGAAAGCGCACAAAAAACTTTCAG
GAAATGTTATAATTAGAAGT

Product: malolactic fermentation system transcription activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSLNLRDLEYFYQLSKLRSFTNVAKHFRVSQPTISYAIKRLETYYDCDLFYKDSSHQVVDLTPEGELLAMRTNEILSELN
HTKKAIHRSSMKDFKVGFPPIISSYLLSKQDNLNFFNALHMIYGGSNELLSLLLNEEIDSSLLGSLEKIKHPQLEIEELF
QKEFFIIMSDSHPLANEKELGFKDLLNEDFILLGEHNIHFNAFNRLNEKYNQSANVVLKLDDAHTIKELVRKNLGISLMA
DIRLSEDFKNLVKVPFIEEDKQFFHINYAYQKNSILSESEKNFLEILKTLK

Sequences:

>Translated_291_residues
MSLNLRDLEYFYQLSKLRSFTNVAKHFRVSQPTISYAIKRLETYYDCDLFYKDSSHQVVDLTPEGELLAMRTNEILSELN
HTKKAIHRSSMKDFKVGFPPIISSYLLSKQDNLNFFNALHMIYGGSNELLSLLLNEEIDSSLLGSLEKIKHPQLEIEELF
QKEFFIIMSDSHPLANEKELGFKDLLNEDFILLGEHNIHFNAFNRLNEKYNQSANVVLKLDDAHTIKELVRKNLGISLMA
DIRLSEDFKNLVKVPFIEEDKQFFHINYAYQKNSILSESEKNFLEILKTLK
>Mature_290_residues
SLNLRDLEYFYQLSKLRSFTNVAKHFRVSQPTISYAIKRLETYYDCDLFYKDSSHQVVDLTPEGELLAMRTNEILSELNH
TKKAIHRSSMKDFKVGFPPIISSYLLSKQDNLNFFNALHMIYGGSNELLSLLLNEEIDSSLLGSLEKIKHPQLEIEELFQ
KEFFIIMSDSHPLANEKELGFKDLLNEDFILLGEHNIHFNAFNRLNEKYNQSANVVLKLDDAHTIKELVRKNLGISLMAD
IRLSEDFKNLVKVPFIEEDKQFFHINYAYQKNSILSESEKNFLEILKTLK

Specific function: Required for malolactic fermentation. It is most probably a transcriptional activator

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=23.972602739726, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790399, Length=302, Percent_Identity=23.5099337748344, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787879, Length=304, Percent_Identity=22.3684210526316, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=249, Percent_Identity=23.6947791164659, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLER_LACLA (P16400)

Other databases:

- EMBL:   M90762
- EMBL:   AE005176
- PIR:   A44510
- PIR:   H86733
- RefSeq:   NP_267028.1
- ProteinModelPortal:   P16400
- GeneID:   1114502
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0148
- NMPDR:   fig|272623.1.peg.894
- HOGENOM:   HBG697416
- OMA:   TNITRAG
- ProtClustDB:   CLSK876798
- BioCyc:   LLAC272623:L0148-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33965; Mature: 33834

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNLRDLEYFYQLSKLRSFTNVAKHFRVSQPTISYAIKRLETYYDCDLFYKDSSHQVVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEE
LTPEGELLAMRTNEILSELNHTKKAIHRSSMKDFKVGFPPIISSYLLSKQDNLNFFNALH
ECCCCCEEEEHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHCCCCCCHHHHHHH
MIYGGSNELLSLLLNEEIDSSLLGSLEKIKHPQLEIEELFQKEFFIIMSDSHPLANEKEL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEEECCCCCCCCCCC
GFKDLLNEDFILLGEHNIHFNAFNRLNEKYNQSANVVLKLDDAHTIKELVRKNLGISLMA
CHHHHCCCCEEEEECCCEEHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCEEEEE
DIRLSEDFKNLVKVPFIEEDKQFFHINYAYQKNSILSESEKNFLEILKTLK
ECEEHHHHHHHHCCCCEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLNLRDLEYFYQLSKLRSFTNVAKHFRVSQPTISYAIKRLETYYDCDLFYKDSSHQVVD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEE
LTPEGELLAMRTNEILSELNHTKKAIHRSSMKDFKVGFPPIISSYLLSKQDNLNFFNALH
ECCCCCEEEEHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHCCCCCCHHHHHHH
MIYGGSNELLSLLLNEEIDSSLLGSLEKIKHPQLEIEELFQKEFFIIMSDSHPLANEKEL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEEECCCCCCCCCCC
GFKDLLNEDFILLGEHNIHFNAFNRLNEKYNQSANVVLKLDDAHTIKELVRKNLGISLMA
CHHHHCCCCEEEEECCCEEHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCEEEEE
DIRLSEDFKNLVKVPFIEEDKQFFHINYAYQKNSILSESEKNFLEILKTLK
ECEEHHHHHHHHCCCCEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2498286; 11337471