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The map label for this gene is pbpX

Identifier: 15672849

GI number: 15672849

Start: 889342

End: 891633

Strand: Direct

Name: pbpX

Synonym: L89079

Alternate gene names: 15672849

Gene position: 889342-891633 (Clockwise)

Preceding gene: 15672848

Following gene: 15672850

Centisome position: 37.59

GC content: 37.04

Gene sequence:

>2292_bases
ATGATAAAATTTTTAAAAAAACTAATTTTCTTTCCTTTTAGAAAAGTATCACGAAATGCCCGAAAAACGAATTGGAGTAC
AGAAAGAAATCGTAAACGAGTAGGTAAGAGCCTGTTTTTCCTAGCTATTGCGCTGTTTACGGTTTTTATATTTCGTTTTG
TCTGGCTAATTACCGTCAATCATGTCGGTGGAACCAATCTTACAACGATGGCCAAGAGCAACTATCAAAGCACAGAAACA
GTTCATGCGAAGCGTGGAACAATATATGACCGTACAGGTGCGGCTATTGCGGTAGACTCAAGTACCTATACAATTTATGC
TGTAATTGATAAAACACAGGTTGATTCAAATGGAAATCCTCTTTATATTGATAAGAAAGATTTTACCAAAGTTGAAGATT
TCTTAAATAGTAAACTTGGAATTGACCGCGATTTAATTAAAAAGCAGCTCAATTCTAAATTGAAACAAGTTCAATTTGGC
CCTAAAGGTAGTGATATCTCTCTTGAAAAAATGAAAGAGATTCAAAAAGCGGCTGAGAATGAAAAAATTGTTGGACTTGG
TTTCACGGCAAATATTTCTCGTTCTTATCCTTTTGGAAATTTTGCTTCTCAATTTATTGGTATTGCTCGTCCTAAAGATG
AAAATGGTACTCAAGCTCTCAAGGGTGATATGGGCTTGGAAAAAGCCTTCAATAATGTTTTGAGTGGTGAAAATGGTAAA
GAAACTTATCAAAAAGATATCTATGGTCGCCCAATTCCAGGAACAACTAAAGTCATTGAACCTGTTAAAAATGGTCAAGA
CGTTTATACTACACTTGATGCACAACTTCAAAGAAATCTTGAAGGATATATGGACAAGGCGGCTACGGATACTGGAGCAC
AACAACTTTCAGGAACTTTAGTTGATGCTCATACCGGTGAGATTTTAGCAACAAGTCAACGTCCAACCTATACGGCAACA
ACGATCAATGATGCTGAGAAACAAAAGTATTTCACTTGGAACAGTTTGCTCTCACAATCAGCCTTTGAACCGGGTTCAAC
ATTTAAAACTTTCTTGATGGCTGGAGCGCTTGATAGTGGAAAAGTAAATCTTAATGAAACTTACCAACGGAAACTTCAAG
TTTATGATACAACGATTAATGACTGGGATGTAACAGAAAATAAAAGTTATACTTTGCCAGAAACCGTAACCTATGCTCAA
GGTTTTGCTTTATCTTCAAATATTGGGATGAGTAAAATTGAAATGAATATGGGAGATGCTTTATGGGGAAGTTATCTTAA
TAAATTTAAATTTGGTCTTAAAGTAAGAGCCGGTTTAGATGGTGAAAATCCGGGAGCCCTTCCTTCGTCAAATGCCGTTT
CGCAAATTCAATCTTCATTTGGTCAAGGGGTTGCGGTTACTCCACTTCAATTGATTAGAGGTTGGACGGCTATTGCTGGA
AATGGAACAATGCTTGAGCCACATATTGTCAGCAAAGTAGTAGATCCGAATACAAAAACAAGTTTGACTTCTAAAGCAGA
AGTTGTTGGACATCCGGTCTCTAATGATGCAGCAAGTGGTGTGCGTGATTTGATGTTAACAGTAAATACTGACCCTGTTT
ACGGAACATCTTATTCAACAAGTGGCGATTCTGAGCAAAATCTTGCTGCTGGTCCTTTATTTATGGTCAATGGTGAACCG
GCTGCGGTTAAAACGGGGACGGCCCAAATTGCCTCAACAACGGGTGGTTATATGACAGGTTCTCAAGATTACCTTTACTC
TGCTGTTGTGATGTATCCTGCTAAGAATCCTGATTTTATTTTCTATATGAATGTAAAAATTCCTACAGAAAGTTGGACAC
TTAAATATATTGCTCGAGTGGCAAATCCATTGTTAACAAGTGCTGAAGCAATGAAAAATGATTTATCAGTTAGTGCTGAT
ACTGATACTAAAGCTGGTAAAGTGACCATTGAGAATTATAAAGGCAAAGATTCAGGAGATACTGCTGATAGCTTACGCCG
CACTGTCCTTAGTCCAGTTATTATCGGGACAGGGGCAAAAGTAACTGCTCAATCGATTGCTGAAGGTGAAAAAGTTTCAG
CAAATTCACGAATTTTACTTTTGACGAATGATAAAGATCAGGTGATGCCTGATATGTATGATTGGTCGAAAAAAGAAGTT
GAGCAATTGGCTAATTGGTTTGGAATTAAAGTAACTTATGAAGGGTCAGGAAATAAAGTCTTGACTCAAAGTATCGAAAC
TTCTACCAATGTTAAAAAAGGTCAAACTCTAACTGTTAAAATGGGAAATTAA

Upstream 100 bases:

>100_bases
ATCAACAAATTTTAGATTTAACATCAGGTACTAAAGTGCTTGATACGGCGAATAAAGCTGATTTAAAACAAAATCCTTCT
AATGTATTGAAAGCAACGAA

Downstream 100 bases:

>100_bases
AAATTATTAATTGAGGCTCCACAGACACTAGTCTATGTGGAGCTTCAAAAATTAGGAGAAGAAAATGTTATTGAATGGGA
TAGTGGCGGCGGTAATTACA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2x; PBP2x [H]

Number of amino acids: Translated: 763; Mature: 763

Protein sequence:

>763_residues
MIKFLKKLIFFPFRKVSRNARKTNWSTERNRKRVGKSLFFLAIALFTVFIFRFVWLITVNHVGGTNLTTMAKSNYQSTET
VHAKRGTIYDRTGAAIAVDSSTYTIYAVIDKTQVDSNGNPLYIDKKDFTKVEDFLNSKLGIDRDLIKKQLNSKLKQVQFG
PKGSDISLEKMKEIQKAAENEKIVGLGFTANISRSYPFGNFASQFIGIARPKDENGTQALKGDMGLEKAFNNVLSGENGK
ETYQKDIYGRPIPGTTKVIEPVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTLVDAHTGEILATSQRPTYTAT
TINDAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSGKVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQ
GFALSSNIGMSKIEMNMGDALWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
NGTMLEPHIVSKVVDPNTKTSLTSKAEVVGHPVSNDAASGVRDLMLTVNTDPVYGTSYSTSGDSEQNLAAGPLFMVNGEP
AAVKTGTAQIASTTGGYMTGSQDYLYSAVVMYPAKNPDFIFYMNVKIPTESWTLKYIARVANPLLTSAEAMKNDLSVSAD
TDTKAGKVTIENYKGKDSGDTADSLRRTVLSPVIIGTGAKVTAQSIAEGEKVSANSRILLLTNDKDQVMPDMYDWSKKEV
EQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN

Sequences:

>Translated_763_residues
MIKFLKKLIFFPFRKVSRNARKTNWSTERNRKRVGKSLFFLAIALFTVFIFRFVWLITVNHVGGTNLTTMAKSNYQSTET
VHAKRGTIYDRTGAAIAVDSSTYTIYAVIDKTQVDSNGNPLYIDKKDFTKVEDFLNSKLGIDRDLIKKQLNSKLKQVQFG
PKGSDISLEKMKEIQKAAENEKIVGLGFTANISRSYPFGNFASQFIGIARPKDENGTQALKGDMGLEKAFNNVLSGENGK
ETYQKDIYGRPIPGTTKVIEPVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTLVDAHTGEILATSQRPTYTAT
TINDAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSGKVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQ
GFALSSNIGMSKIEMNMGDALWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
NGTMLEPHIVSKVVDPNTKTSLTSKAEVVGHPVSNDAASGVRDLMLTVNTDPVYGTSYSTSGDSEQNLAAGPLFMVNGEP
AAVKTGTAQIASTTGGYMTGSQDYLYSAVVMYPAKNPDFIFYMNVKIPTESWTLKYIARVANPLLTSAEAMKNDLSVSAD
TDTKAGKVTIENYKGKDSGDTADSLRRTVLSPVIIGTGAKVTAQSIAEGEKVSANSRILLLTNDKDQVMPDMYDWSKKEV
EQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN
>Mature_763_residues
MIKFLKKLIFFPFRKVSRNARKTNWSTERNRKRVGKSLFFLAIALFTVFIFRFVWLITVNHVGGTNLTTMAKSNYQSTET
VHAKRGTIYDRTGAAIAVDSSTYTIYAVIDKTQVDSNGNPLYIDKKDFTKVEDFLNSKLGIDRDLIKKQLNSKLKQVQFG
PKGSDISLEKMKEIQKAAENEKIVGLGFTANISRSYPFGNFASQFIGIARPKDENGTQALKGDMGLEKAFNNVLSGENGK
ETYQKDIYGRPIPGTTKVIEPVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTLVDAHTGEILATSQRPTYTAT
TINDAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSGKVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQ
GFALSSNIGMSKIEMNMGDALWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
NGTMLEPHIVSKVVDPNTKTSLTSKAEVVGHPVSNDAASGVRDLMLTVNTDPVYGTSYSTSGDSEQNLAAGPLFMVNGEP
AAVKTGTAQIASTTGGYMTGSQDYLYSAVVMYPAKNPDFIFYMNVKIPTESWTLKYIARVANPLLTSAEAMKNDLSVSAD
TDTKAGKVTIENYKGKDSGDTADSLRRTVLSPVIIGTGAKVTAQSIAEGEKVSANSRILLLTNDKDQVMPDMYDWSKKEV
EQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=583, Percent_Identity=26.0720411663808, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1786854, Length=572, Percent_Identity=21.1538461538462, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 83442; Mature: 83442

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFLKKLIFFPFRKVSRNARKTNWSTERNRKRVGKSLFFLAIALFTVFIFRFVWLITVN
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
HVGGTNLTTMAKSNYQSTETVHAKRGTIYDRTGAAIAVDSSTYTIYAVIDKTQVDSNGNP
CCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCEEEECCCCEEEEEEEEEEEECCCCCE
LYIDKKDFTKVEDFLNSKLGIDRDLIKKQLNSKLKQVQFGPKGSDISLEKMKEIQKAAEN
EEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCC
EKIVGLGFTANISRSYPFGNFASQFIGIARPKDENGTQALKGDMGLEKAFNNVLSGENGK
CEEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCH
ETYQKDIYGRPIPGTTKVIEPVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTL
HHHHHHCCCCCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHCCEE
VDAHTGEILATSQRPTYTATTINDAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSG
EECCCCCEEEECCCCCEEEEECCCHHHHHEEEHHHHHHHHCCCCCCHHHHHHHHCCCCCC
KVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQGFALSSNIGMSKIEMNMGDA
CCCCCHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCEEEEECCCHH
LWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECHHHHHCCCEEEEC
NGTMLEPHIVSKVVDPNTKTSLTSKAEVVGHPVSNDAASGVRDLMLTVNTDPVYGTSYST
CCCEECHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHCCEEEEEEEECCCCCCCCCCC
SGDSEQNLAAGPLFMVNGEPAAVKTGTAQIASTTGGYMTGSQDYLYSAVVMYPAKNPDFI
CCCCCCCCCCCCEEEECCCCCEEECCCEEEEECCCCCCCCCHHHEEEEEEEECCCCCCEE
FYMNVKIPTESWTLKYIARVANPLLTSAEAMKNDLSVSADTDTKAGKVTIENYKGKDSGD
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCCC
TADSLRRTVLSPVIIGTGAKVTAQSIAEGEKVSANSRILLLTNDKDQVMPDMYDWSKKEV
HHHHHHHHHHCCEEECCCCCHHHHHHHCCCEECCCCEEEEEECCCHHCCCCHHCCCHHHH
EQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN
HHHHHHEEEEEEEECCCCEEEEECHHCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MIKFLKKLIFFPFRKVSRNARKTNWSTERNRKRVGKSLFFLAIALFTVFIFRFVWLITVN
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
HVGGTNLTTMAKSNYQSTETVHAKRGTIYDRTGAAIAVDSSTYTIYAVIDKTQVDSNGNP
CCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCEEEECCCCEEEEEEEEEEEECCCCCE
LYIDKKDFTKVEDFLNSKLGIDRDLIKKQLNSKLKQVQFGPKGSDISLEKMKEIQKAAEN
EEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCC
EKIVGLGFTANISRSYPFGNFASQFIGIARPKDENGTQALKGDMGLEKAFNNVLSGENGK
CEEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCH
ETYQKDIYGRPIPGTTKVIEPVKNGQDVYTTLDAQLQRNLEGYMDKAATDTGAQQLSGTL
HHHHHHCCCCCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHCCEE
VDAHTGEILATSQRPTYTATTINDAEKQKYFTWNSLLSQSAFEPGSTFKTFLMAGALDSG
EECCCCCEEEECCCCCEEEEECCCHHHHHEEEHHHHHHHHCCCCCCHHHHHHHHCCCCCC
KVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYAQGFALSSNIGMSKIEMNMGDA
CCCCCHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCEEEEECCCHH
LWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVSQIQSSFGQGVAVTPLQLIRGWTAIAG
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEECHHHHHCCCEEEEC
NGTMLEPHIVSKVVDPNTKTSLTSKAEVVGHPVSNDAASGVRDLMLTVNTDPVYGTSYST
CCCEECHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHCCEEEEEEEECCCCCCCCCCC
SGDSEQNLAAGPLFMVNGEPAAVKTGTAQIASTTGGYMTGSQDYLYSAVVMYPAKNPDFI
CCCCCCCCCCCCEEEECCCCCEEECCCEEEEECCCCCCCCCHHHEEEEEEEECCCCCCEE
FYMNVKIPTESWTLKYIARVANPLLTSAEAMKNDLSVSADTDTKAGKVTIENYKGKDSGD
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCCC
TADSLRRTVLSPVIIGTGAKVTAQSIAEGEKVSANSRILLLTNDKDQVMPDMYDWSKKEV
HHHHHHHHHHCCEEECCCCCHHHHHHHCCCEECCCCEEEEEECCCHHCCCCHHCCCHHHH
EQLANWFGIKVTYEGSGNKVLTQSIETSTNVKKGQTLTVKMGN
HHHHHHEEEEEEEECCCCEEEEECHHCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916; 8605631 [H]