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The map label for this gene is thrA

Identifier: 15672722

GI number: 15672722

Start: 747291

End: 748643

Strand: Reverse

Name: thrA

Synonym: L0097

Alternate gene names: 15672722

Gene position: 748643-747291 (Counterclockwise)

Preceding gene: 15672723

Following gene: 15672718

Centisome position: 31.65

GC content: 37.84

Gene sequence:

>1353_bases
GTGAAAGTTGCAAAATTTGGAGGTTCGTCTCTCGCATCAGCTTCGCAAATCAAAAAAGTTTTTGATATTGTTCAAGCCGA
TAAACAAAGAAAATTTATTGTGGTTTCTGCACCAGGCAAACGTTCGGGGGCTGATACTAAAGTTACCGATGCTTTGATTG
CTTATTATAAAGCTTATAAATCAGCTGATGAAAAAGGAATTCACTATTATCAGAATTGGATTATTGAAAGATTTCGAGAA
ATCTATGATGAACTTGGCTTAAAAAGTCCAGTCATTAATCAAATCGCAGAAAATATCAACCATTTAGCCCAGTTAGATTT
AGATAATGTTTTTACTTATGATACTTTTTTAGCCGCTGGTGAAAATAATAATGCTAAATTAGTCGCAGATTTTTTCAATC
ATTCTGGCTTACCAAGTCGCTATGTTCATCCATCAGATGCTGGAATTATGGTTTCATCTGATCCTGGAAATGCTCGATTG
CTTGGTGGAGCTTATGAACATATTAAATATCTTAATGAACTTGAAGAAACATTAATCATCCCTGGTTTTTTTGGAATCAC
AAAAGAGGGTGAGGTTTGCACTTTTTCCCGTGGTGGTTCTGATATTACTGGCTCAATTATTGCGGCTGGTGTGCGAGCTG
AACTCTATGAAAACTTTACTGATGTGAATGGAATTTTCGCTGCTCACCCTGGCATCGTTAAGAATCCAGCTTATATCTCT
GAATTAACTTACCGTGAAATGCGAGAACTTGCTTATTCTGGTTTTTCTGTTTTACATGACGAAGCACTCGTTCCTGCCTA
TCGTGCCCATGTTCCTTTGGTCATCAAGAATACAAATAACCCTGATCATCCCGGAACAAAAATTGTTGTTCAAAGAGAGA
CTAAAGGCTCACCTGTCGTTGGAATTGCTTCATCTAGTGCCTTCTCTTCTCTTTATGTCAGTAAATATCTGATGAACCGT
GAAGTTGGATTCGGCCGAAAAGTCCTTTCTGTATTGGAAAATTTAGGAGTTCGTTTTGAGCATATGCCAACTGGTATTGA
TGACATGTCTATCATTATTCGCAGCCGCTATCTAACTCAACCTATTTCAAAAGCCCTTATTTCTAATCTTACAGAATCGC
TCCATCCGGATGAAGTCTATATTGACCGTGATTTCTCTATTATCACCATTGTTGGTGAAGATATGAAAGAACACGTTGGG
GTTACCGCTAGAGCAACGGCAGCACTTTCTAAACAGGGCATTTCAATCGCTATGCTTTCGCAAGGTTCAAGCGAAGTTTC
CGTCATGTTTATTGTCAAAGAAAAATACGAAAAAGAAGCTCTAAAAGCGATTTATTCAGCCTTTTTTGACTAA

Upstream 100 bases:

>100_bases
AAATAGGCTATAAATGAGTTATTTATTGAAAATTATCTGAAAATTAGCTATAATAAATCAATTTATAATTTAATTTAATC
TTGAAGGAAGGTAATATTTA

Downstream 100 bases:

>100_bases
AATATGTTAATAAAAAACAGCTCTAAGAGCTGTTTTTTTATTCTTCTATTTTTTCGACTTTTTCAAGTGTCTCATTGGAA
TTACCACGACTGCTAAAATT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKVAKFGGSSLASASQIKKVFDIVQADKQRKFIVVSAPGKRSGADTKVTDALIAYYKAYKSADEKGIHYYQNWIIERFRE
IYDELGLKSPVINQIAENINHLAQLDLDNVFTYDTFLAAGENNNAKLVADFFNHSGLPSRYVHPSDAGIMVSSDPGNARL
LGGAYEHIKYLNELEETLIIPGFFGITKEGEVCTFSRGGSDITGSIIAAGVRAELYENFTDVNGIFAAHPGIVKNPAYIS
ELTYREMRELAYSGFSVLHDEALVPAYRAHVPLVIKNTNNPDHPGTKIVVQRETKGSPVVGIASSSAFSSLYVSKYLMNR
EVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQPISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVG
VTARATAALSKQGISIAMLSQGSSEVSVMFIVKEKYEKEALKAIYSAFFD

Sequences:

>Translated_450_residues
MKVAKFGGSSLASASQIKKVFDIVQADKQRKFIVVSAPGKRSGADTKVTDALIAYYKAYKSADEKGIHYYQNWIIERFRE
IYDELGLKSPVINQIAENINHLAQLDLDNVFTYDTFLAAGENNNAKLVADFFNHSGLPSRYVHPSDAGIMVSSDPGNARL
LGGAYEHIKYLNELEETLIIPGFFGITKEGEVCTFSRGGSDITGSIIAAGVRAELYENFTDVNGIFAAHPGIVKNPAYIS
ELTYREMRELAYSGFSVLHDEALVPAYRAHVPLVIKNTNNPDHPGTKIVVQRETKGSPVVGIASSSAFSSLYVSKYLMNR
EVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQPISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVG
VTARATAALSKQGISIAMLSQGSSEVSVMFIVKEKYEKEALKAIYSAFFD
>Mature_450_residues
MKVAKFGGSSLASASQIKKVFDIVQADKQRKFIVVSAPGKRSGADTKVTDALIAYYKAYKSADEKGIHYYQNWIIERFRE
IYDELGLKSPVINQIAENINHLAQLDLDNVFTYDTFLAAGENNNAKLVADFFNHSGLPSRYVHPSDAGIMVSSDPGNARL
LGGAYEHIKYLNELEETLIIPGFFGITKEGEVCTFSRGGSDITGSIIAAGVRAELYENFTDVNGIFAAHPGIVKNPAYIS
ELTYREMRELAYSGFSVLHDEALVPAYRAHVPLVIKNTNNPDHPGTKIVVQRETKGSPVVGIASSSAFSSLYVSKYLMNR
EVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQPISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVG
VTARATAALSKQGISIAMLSQGSSEVSVMFIVKEKYEKEALKAIYSAFFD

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=480, Percent_Identity=25, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1790455, Length=472, Percent_Identity=23.9406779661017, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790376, Length=464, Percent_Identity=22.4137931034483, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=379, Percent_Identity=25.5936675461741, Blast_Score=108, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49593; Mature: 49593

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAKFGGSSLASASQIKKVFDIVQADKQRKFIVVSAPGKRSGADTKVTDALIAYYKAYK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
SADEKGIHYYQNWIIERFREIYDELGLKSPVINQIAENINHLAQLDLDNVFTYDTFLAAG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHEEEEC
ENNNAKLVADFFNHSGLPSRYVHPSDAGIMVSSDPGNARLLGGAYEHIKYLNELEETLII
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHEEC
PGFFGITKEGEVCTFSRGGSDITGSIIAAGVRAELYENFTDVNGIFAAHPGIVKNPAYIS
CCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
ELTYREMRELAYSGFSVLHDEALVPAYRAHVPLVIKNTNNPDHPGTKIVVQRETKGSPVV
HHHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCEE
GIASSSAFSSLYVSKYLMNREVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQ
EEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
PISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVGVTARATAALSKQGISIAMLS
HHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCHHHHCCCCHHHHHHHHHCCCEEEEEC
QGSSEVSVMFIVKEKYEKEALKAIYSAFFD
CCCCCEEEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVAKFGGSSLASASQIKKVFDIVQADKQRKFIVVSAPGKRSGADTKVTDALIAYYKAYK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
SADEKGIHYYQNWIIERFREIYDELGLKSPVINQIAENINHLAQLDLDNVFTYDTFLAAG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHEEEEC
ENNNAKLVADFFNHSGLPSRYVHPSDAGIMVSSDPGNARLLGGAYEHIKYLNELEETLII
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHEEC
PGFFGITKEGEVCTFSRGGSDITGSIIAAGVRAELYENFTDVNGIFAAHPGIVKNPAYIS
CCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
ELTYREMRELAYSGFSVLHDEALVPAYRAHVPLVIKNTNNPDHPGTKIVVQRETKGSPVV
HHHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCEE
GIASSSAFSSLYVSKYLMNREVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQ
EEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
PISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVGVTARATAALSKQGISIAMLS
HHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCHHHHCCCCHHHHHHHHHCCCEEEEEC
QGSSEVSVMFIVKEKYEKEALKAIYSAFFD
CCCCCEEEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]