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The map label for this gene is celB

Identifier: 15672156

GI number: 15672156

Start: 177530

End: 179014

Strand: Direct

Name: celB

Synonym: L177520

Alternate gene names: 15672156

Gene position: 177530-179014 (Clockwise)

Preceding gene: 15672155

Following gene: 30023977

Centisome position: 7.5

GC content: 43.77

Gene sequence:

>1485_bases
ATGAACGGAATTACTGCGTGGATGGAGAAATATCTCGTCCCTGTTGCGGCAAAGATTGGGTCTCAAAAACACCTAGTTGC
GCTGCGTGACTCATTTATCGGTATGTTGCCAGCAACACTTGCTGGTGCCTTAGCTGCCATGATTTCAGCTATCGTGACAA
CATTCCCGTCAGCTATCCAACAAATGATGTTGGGAGCAACAGCATTCTCAAAATTAGCACCAGAGAAAGTCTGGACACTT
GCTAATACACCAATCATTGGTGATTTGAACAATATTTCAGCCCTTGTTAACCAAGGAACACTGACAGTTATTGGTCTTAT
CTTTGCCTTTTCTTGGGGTTACAATTTGGCCCGCGCTTACGGAGTCAATGACCTCGCTGGTGGTATCGTATCACTTGCAA
CCTTATTTGCAGGTTTGCCAAACCAAATGGGTAAATTTACAGCAGCCCTTGGTACAGGTAAAGCTGGTGTAGCAGCAACT
GACAAGCTTAATGGCGTTCTTGGTGATCAAGGACTTGCCGCTTGGAAACCACTCTTTGCCTCAGCTCACCTCGATGCTGG
AGCTTACTTCACAGTTATTATTATGGGTGCCCTTGCAGTTATTATTTATGCAAAACTCATGCTTGCTGACATCACAATTA
AGATGCCAGAATCAGTTCCGCCTGCAGTAGCAAAAGCTTTCTTAGCAATCATTCCTACAATTGCAGCACTTTATATCGTT
GGTCTAATCTACTACATTATTGGTAAATTGACAAATGACTCTGTCATTAACCTGATTACCCACTATATCGCAGAGCCATT
CCAAATCCTGTCACAAAATATCTTCTCAGTACTGATTGTTACACTCTTTGTTTCAGTCTTCTGGTTCTTCGGTCTTCACG
GACCAAACGTTTTGGCACCAGTACTCGATGGTATCTGGGGACCTCTTGGACTTAATAACCAAGCCCTCTACTTCCAAGTT
CACTCACAAGGTATCCGTGACTTGATTGCTAAAGGTGCGGTTGACAAAGCACATGCCATTAATGGTGACTACGTTAACCT
TTGGGTACGTGGTTCATGGGACGCCTTTGCATGGTTTGGTGGATCAGGTGGTACAATTACACTTGTTATTGCAATCATTC
TCTTCTCTAAACGTAAAGACTACAAGATTGTTGGACGTCTCGGACTTGCACCTGGTATCTTCAACATCAACGAACCAGTC
CTCTTCGGTCTGCCAGTTGTCTTGAATGCAATCTTCTTTATTCCATTTGCAGTCGCTCCATTGATTTCTGTAATCATTGC
CTACACTGCAACAGCCCTTCACTTGGTTGACCCAGTTGTCAATGCTGTGCCATGGGTAACACCACCAATTATGAATGCCT
TCATGGCAACCGGTTTTGACTGGCGGGCAATTGTCCTTACAATTATCAACTTGATCATTACCTTTGTTATCTGGGTACCA
TTCGTTATCGCAGCTAACAAGCTGGAAGAAACTGAACTCGACTAA

Upstream 100 bases:

>100_bases
TAATCGATAATCTATGCCTGTCAGTACTGACAGAACCTTAATTATGTTCAAAAAGTTTTTTTGAAAGTTATTTATTATTA
TATTATAAAGGAGGAATTCC

Downstream 100 bases:

>100_bases
ATTTGAAATTAATGGTAGAGCAAGTAAGAAAACTTGCTCTACACATTTTTTGTTAAATCTGGTAAGATTTATAATATAAA
CCTTATCAGATGTGATAAAA

Product: cellobiose-specific PTS system IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Glucomannan utilization protein C; PTS system oligo-beta-mannoside-specific EIIC component [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNGITAWMEKYLVPVAAKIGSQKHLVALRDSFIGMLPATLAGALAAMISAIVTTFPSAIQQMMLGATAFSKLAPEKVWTL
ANTPIIGDLNNISALVNQGTLTVIGLIFAFSWGYNLARAYGVNDLAGGIVSLATLFAGLPNQMGKFTAALGTGKAGVAAT
DKLNGVLGDQGLAAWKPLFASAHLDAGAYFTVIIMGALAVIIYAKLMLADITIKMPESVPPAVAKAFLAIIPTIAALYIV
GLIYYIIGKLTNDSVINLITHYIAEPFQILSQNIFSVLIVTLFVSVFWFFGLHGPNVLAPVLDGIWGPLGLNNQALYFQV
HSQGIRDLIAKGAVDKAHAINGDYVNLWVRGSWDAFAWFGGSGGTITLVIAIILFSKRKDYKIVGRLGLAPGIFNINEPV
LFGLPVVLNAIFFIPFAVAPLISVIIAYTATALHLVDPVVNAVPWVTPPIMNAFMATGFDWRAIVLTIINLIITFVIWVP
FVIAANKLEETELD

Sequences:

>Translated_494_residues
MNGITAWMEKYLVPVAAKIGSQKHLVALRDSFIGMLPATLAGALAAMISAIVTTFPSAIQQMMLGATAFSKLAPEKVWTL
ANTPIIGDLNNISALVNQGTLTVIGLIFAFSWGYNLARAYGVNDLAGGIVSLATLFAGLPNQMGKFTAALGTGKAGVAAT
DKLNGVLGDQGLAAWKPLFASAHLDAGAYFTVIIMGALAVIIYAKLMLADITIKMPESVPPAVAKAFLAIIPTIAALYIV
GLIYYIIGKLTNDSVINLITHYIAEPFQILSQNIFSVLIVTLFVSVFWFFGLHGPNVLAPVLDGIWGPLGLNNQALYFQV
HSQGIRDLIAKGAVDKAHAINGDYVNLWVRGSWDAFAWFGGSGGTITLVIAIILFSKRKDYKIVGRLGLAPGIFNINEPV
LFGLPVVLNAIFFIPFAVAPLISVIIAYTATALHLVDPVVNAVPWVTPPIMNAFMATGFDWRAIVLTIINLIITFVIWVP
FVIAANKLEETELD
>Mature_494_residues
MNGITAWMEKYLVPVAAKIGSQKHLVALRDSFIGMLPATLAGALAAMISAIVTTFPSAIQQMMLGATAFSKLAPEKVWTL
ANTPIIGDLNNISALVNQGTLTVIGLIFAFSWGYNLARAYGVNDLAGGIVSLATLFAGLPNQMGKFTAALGTGKAGVAAT
DKLNGVLGDQGLAAWKPLFASAHLDAGAYFTVIIMGALAVIIYAKLMLADITIKMPESVPPAVAKAFLAIIPTIAALYIV
GLIYYIIGKLTNDSVINLITHYIAEPFQILSQNIFSVLIVTLFVSVFWFFGLHGPNVLAPVLDGIWGPLGLNNQALYFQV
HSQGIRDLIAKGAVDKAHAINGDYVNLWVRGSWDAFAWFGGSGGTITLVIAIILFSKRKDYKIVGRLGLAPGIFNINEPV
LFGLPVVLNAIFFIPFAVAPLISVIIAYTATALHLVDPVVNAVPWVTPPIMNAFMATGFDWRAIVLTIINLIITFVIWVP
FVIAANKLEETELD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=500, Percent_Identity=31, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 52780; Mature: 52780

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCHHCCCC
>Mature Secondary Structure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HHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]