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The map label for this gene is pyrE [C]

Identifier: 15669842

GI number: 15669842

Start: 1628436

End: 1629071

Strand: Direct

Name: pyrE [C]

Synonym: MJ1646

Alternate gene names: 15669842

Gene position: 1628436-1629071 (Clockwise)

Preceding gene: 15669841

Following gene: 15669843

Centisome position: 97.81

GC content: 32.23

Gene sequence:

>636_bases
ATGAGGGAGATTATGAATAAAGAATTATTAAAAAAGGTTATAGAGTTAAAAAGTAATGGACTTACAATTGGAGAGATTGC
TGAGGAACTAAATGTATCAATGGAGACAGCAAGATATTTGGTTTTAAATGCTGAGAAATTGTTAAAAGAGGAGGAAAAAG
CTATAAAGCTTGAAAATGTTGATATATTCATTGATTGGAAAAATATTGGTAGCTCAGCAAATAGGTTAAAATACATTAGC
TCAATAATAGTTGATATTTTAAAGAGCAGGAATATCGAATTCGATACCGTTGTTGGAGTCTCTACAAGTGGAGTTCCTAT
AGCTACTTTGGTAGCTTCCGAATTAGGTAAAGAATTAACTATCTACATACCAAAGAAGCATATATCTGAAGAAGGGAAAA
AGATAACTGGTTCAATATCACAAAACTTCTCAGCTGTAAATTACAAGAGAGCTGTTATCATAGATGATGTTGTAACAAGT
GGAAGCACTTTAAAGGAGTGTATAAAGCAGTTGAAAGAAGTATGCAGCCCAAAATTAGTAGTTGTATTGATAGATAAAAG
TGGATTGGATGAGATTGAAGGCGTTCCTTTAATTCCATTGATTAGAATAGGGGCTGTAAATGTAGAGCAAAAATAA

Upstream 100 bases:

>100_bases
GGAAGCATGTGGAGAGTATTTGAAAGATAATGTTATGCTAACAATACAAATGCATAAGTATTTGGGGATGTTATAACATA
TACTGTAAAATCATTATTGA

Downstream 100 bases:

>100_bases
AATAAACATTTTATTATCTTTATTTTTATTATTTTTATTTCAAAAATTTTTGGAGGTATATATTTATGTTACCAAAAGCT
ACAGTAAAGAGGATTATGAA

Product: orotate phosphoribosyltransferase-like protein

Products: orotate; 5-phospho-alpha-D-ribose 1-diphosphate

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MREIMNKELLKKVIELKSNGLTIGEIAEELNVSMETARYLVLNAEKLLKEEEKAIKLENVDIFIDWKNIGSSANRLKYIS
SIIVDILKSRNIEFDTVVGVSTSGVPIATLVASELGKELTIYIPKKHISEEGKKITGSISQNFSAVNYKRAVIIDDVVTS
GSTLKECIKQLKEVCSPKLVVVLIDKSGLDEIEGVPLIPLIRIGAVNVEQK

Sequences:

>Translated_211_residues
MREIMNKELLKKVIELKSNGLTIGEIAEELNVSMETARYLVLNAEKLLKEEEKAIKLENVDIFIDWKNIGSSANRLKYIS
SIIVDILKSRNIEFDTVVGVSTSGVPIATLVASELGKELTIYIPKKHISEEGKKITGSISQNFSAVNYKRAVIIDDVVTS
GSTLKECIKQLKEVCSPKLVVVLIDKSGLDEIEGVPLIPLIRIGAVNVEQK
>Mature_211_residues
MREIMNKELLKKVIELKSNGLTIGEIAEELNVSMETARYLVLNAEKLLKEEEKAIKLENVDIFIDWKNIGSSANRLKYIS
SIIVDILKSRNIEFDTVVGVSTSGVPIATLVASELGKELTIYIPKKHISEEGKKITGSISQNFSAVNYKRAVIIDDVVTS
GSTLKECIKQLKEVCSPKLVVVLIDKSGLDEIEGVPLIPLIRIGAVNVEQK

Specific function: Pyrimidine biosynthesis; fifth step. [C]

COG id: COG0856

COG function: function code F; Orotate phosphoribosyltransferase homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYREL_METJA (Q59040)

Other databases:

- EMBL:   L77117
- PIR:   D64505
- RefSeq:   NP_248656.1
- ProteinModelPortal:   Q59040
- GeneID:   1452555
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1646
- NMPDR:   fig|243232.1.peg.1693
- TIGR:   MJ1646
- HOGENOM:   HBG497993
- OMA:   KDVHIDW
- ProtClustDB:   PRK02277
- BioCyc:   MJAN243232:MJ_1646-MONOMER
- HAMAP:   MF_01214
- InterPro:   IPR022854
- InterPro:   IPR000836
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00156 Pribosyltran

EC number: 2.4.2.10

Molecular weight: Translated: 23307; Mature: 23307

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIMNKELLKKVIELKSNGLTIGEIAEELNVSMETARYLVLNAEKLLKEEEKAIKLENV
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHEECHHHHHHHHHHHEEEECE
DIFIDWKNIGSSANRLKYISSIIVDILKSRNIEFDTVVGVSTSGVPIATLVASELGKELT
EEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCEEE
IYIPKKHISEEGKKITGSISQNFSAVNYKRAVIIDDVVTSGSTLKECIKQLKEVCSPKLV
EEECHHHHHCCCCEEECHHHCCCCCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHCCCCEE
VVLIDKSGLDEIEGVPLIPLIRIGAVNVEQK
EEEECCCCCCHHCCCCCCHHEEECCCCCCCC
>Mature Secondary Structure
MREIMNKELLKKVIELKSNGLTIGEIAEELNVSMETARYLVLNAEKLLKEEEKAIKLENV
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHEECHHHHHHHHHHHEEEECE
DIFIDWKNIGSSANRLKYISSIIVDILKSRNIEFDTVVGVSTSGVPIATLVASELGKELT
EEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCEEE
IYIPKKHISEEGKKITGSISQNFSAVNYKRAVIIDDVVTSGSTLKECIKQLKEVCSPKLV
EEECHHHHHCCCCEEECHHHCCCCCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHCCCCEE
VVLIDKSGLDEIEGVPLIPLIRIGAVNVEQK
EEEECCCCCCHHCCCCCCHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ba2+; Ca2+; Co2+; Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.22 {diphosphate}} 0.013 {diphosphate}} 0.0031 {orotidine} 0.44 {orotate}} 0.03 {orotate}} 0.36 {phosphoribose} 0.038 {phosphoribose} [C]

Substrates: orotidine 5'-phosphate; diphosphate

Specific reaction: orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate

General reaction: Glycosyl and Pentosyl group transfer [C]

Inhibitor: HgCl2; IAA; NEM; PCMB; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087