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The map label for this gene is guaB

Identifier: 15669812

GI number: 15669812

Start: 1591573

End: 1593063

Strand: Direct

Name: guaB

Synonym: MJ1616

Alternate gene names: 15669812

Gene position: 1591573-1593063 (Clockwise)

Preceding gene: 15669808

Following gene: 15669813

Centisome position: 95.59

GC content: 38.63

Gene sequence:

>1491_bases
TTGTTTTTAAAAAAACTAATTGAGGCAAAGAAGGCATATACTTTTGATGATGTTTTATTAGTTCCAAATGCCTCATGGGT
AGAGCCAAAGGATACTGATGTTTCTACAGACTTAGCGGGTTTAAAGTTAAACATCCCTATAGTTTCTGCGGCAATGGATA
CAGTAACAGAAAAAGAGATGGCTATTGCTTTAGCAAGATTAGGAGGTTTAGGAGTTATACATAGAAACATGTCCATAGAG
GAACAAGTTCATCAAGTTCAGGCAGTTAAAAAAGCTGATGAAGTAGTTATTAAGGATGTTATCACCGTATCTCCAGATGA
TACTGTTGGAGAGGCAATAAATGTAATGGAAACATACTCCATCAGCGGATTACCAGTTGTTGATAATGAAGATAAATTAG
TTGGGATTATAACACACAGAGATGTTAAAGCCATTGAAGATAAAACAAAGAAGGTTAAAGATGTAATGACTAAAGATGTC
GTTTGTGCTAAGGAAGATGTTGAAGAAGAGGAAGCTTTAGAGTTGATGTATGCAAATAGGGTTGAAAGATTACCTATAGT
TGATGATGAAAATAGACTAATTGGTATTATAACCTTGAGAGATATTTTAAAGAGAAGAAAGTATCCTCAAGCTGCAAGGG
ATAAAAAAGGTAGATTGTTAGTTGCTGCTGCCTGCGGACCACATGACTTTGAGAGGGCTAAGGCATTGATTGAAGCAGAG
GTTGATGCCATTGCCATTGACTGTGCTCATGCCCACAACATGAGGGTTGTTGAAAATGTAAAGAAATTTAAAGAGATGTT
AGAAGGGACTGACATAAAATTAATAGTTGGGAATATAGCAACTAAAGAAGCGGCAGAGGATTTAATTAAAGCTGGAGCAG
ACGTTTTAAAAGTTGGAATAGGGCCAGGTTCAATCTGCACAACAAGAGTTGTTGCTGGAGTAGGAGTTCCTCAATTAACA
GCCGTTGCTGAAGTGGCTGATGTTGCTAAAGAACATAACGTTCCAATTATAGCAGATGGAGGAATAAGATACAGTGGAGA
TATTGCCAAAGCTATAGCAGCTGGAGCAGATGCGGTTATGCTTGGCTCACTGTTAGCTGGAACTGATGAAGCTCCTGGGC
AGTTAATGGTTATCAATGGAAGGAAGTACAAGCAGTATAGAGGAATGGGGTCATTAGGAGCAATGACAGGAGGAGTTGGA
GCTGGGGCTGATAGATACTTCCAAGCACCAGCAAAAAGCCACATGAAACATGTAAAATTAGTTCCTGAGGGTGTTGAGGG
AGCTGTTCCTTATAAAGGGCCTGTAAGTGAAGTAGTATTCCAATTAATTGGTGGTTTGAGAGCTTCAATGGGATACTGTG
GAGCTAAAAACCTAAAAGAAATGCAAGAAAAGGCAAGATTCGTAATAATAACACCAAGTGGGCAAGTTGAAAGTCATCCT
CACGATATAATTATAACTAACGAAGCTCCGAATTATCCACTGGGTAAATAA

Upstream 100 bases:

>100_bases
TTCATAAACTATTTAAATAATCATATACTCAAGGGCATCATATAAAAAGTATAAATACCCCTCTTCTAAATATAGTTAAA
AAGGTTATAAAGGTGAGACT

Downstream 100 bases:

>100_bases
ATCTAAATTTTATTTTTAATTTTTAAAAAAGGGATGATTATGAAGCCAATATTAATATTTTTAGGGATTATTTTAATGTT
TATTGGATTTTTTATGATAA

Product: inosine-5'-monophosphate dehydrogenase GuaB

Products: NA

Alternate protein names: IMP dehydrogenase; IMPD; IMPDH

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MFLKKLIEAKKAYTFDDVLLVPNASWVEPKDTDVSTDLAGLKLNIPIVSAAMDTVTEKEMAIALARLGGLGVIHRNMSIE
EQVHQVQAVKKADEVVIKDVITVSPDDTVGEAINVMETYSISGLPVVDNEDKLVGIITHRDVKAIEDKTKKVKDVMTKDV
VCAKEDVEEEEALELMYANRVERLPIVDDENRLIGIITLRDILKRRKYPQAARDKKGRLLVAAACGPHDFERAKALIEAE
VDAIAIDCAHAHNMRVVENVKKFKEMLEGTDIKLIVGNIATKEAAEDLIKAGADVLKVGIGPGSICTTRVVAGVGVPQLT
AVAEVADVAKEHNVPIIADGGIRYSGDIAKAIAAGADAVMLGSLLAGTDEAPGQLMVINGRKYKQYRGMGSLGAMTGGVG
AGADRYFQAPAKSHMKHVKLVPEGVEGAVPYKGPVSEVVFQLIGGLRASMGYCGAKNLKEMQEKARFVIITPSGQVESHP
HDIIITNEAPNYPLGK

Sequences:

>Translated_496_residues
MFLKKLIEAKKAYTFDDVLLVPNASWVEPKDTDVSTDLAGLKLNIPIVSAAMDTVTEKEMAIALARLGGLGVIHRNMSIE
EQVHQVQAVKKADEVVIKDVITVSPDDTVGEAINVMETYSISGLPVVDNEDKLVGIITHRDVKAIEDKTKKVKDVMTKDV
VCAKEDVEEEEALELMYANRVERLPIVDDENRLIGIITLRDILKRRKYPQAARDKKGRLLVAAACGPHDFERAKALIEAE
VDAIAIDCAHAHNMRVVENVKKFKEMLEGTDIKLIVGNIATKEAAEDLIKAGADVLKVGIGPGSICTTRVVAGVGVPQLT
AVAEVADVAKEHNVPIIADGGIRYSGDIAKAIAAGADAVMLGSLLAGTDEAPGQLMVINGRKYKQYRGMGSLGAMTGGVG
AGADRYFQAPAKSHMKHVKLVPEGVEGAVPYKGPVSEVVFQLIGGLRASMGYCGAKNLKEMQEKARFVIITPSGQVESHP
HDIIITNEAPNYPLGK
>Mature_496_residues
MFLKKLIEAKKAYTFDDVLLVPNASWVEPKDTDVSTDLAGLKLNIPIVSAAMDTVTEKEMAIALARLGGLGVIHRNMSIE
EQVHQVQAVKKADEVVIKDVITVSPDDTVGEAINVMETYSISGLPVVDNEDKLVGIITHRDVKAIEDKTKKVKDVMTKDV
VCAKEDVEEEEALELMYANRVERLPIVDDENRLIGIITLRDILKRRKYPQAARDKKGRLLVAAACGPHDFERAKALIEAE
VDAIAIDCAHAHNMRVVENVKKFKEMLEGTDIKLIVGNIATKEAAEDLIKAGADVLKVGIGPGSICTTRVVAGVGVPQLT
AVAEVADVAKEHNVPIIADGGIRYSGDIAKAIAAGADAVMLGSLLAGTDEAPGQLMVINGRKYKQYRGMGSLGAMTGGVG
AGADRYFQAPAKSHMKHVKLVPEGVEGAVPYKGPVSEVVFQLIGGLRASMGYCGAKNLKEMQEKARFVIITPSGQVESHP
HDIIITNEAPNYPLGK

Specific function: IMP is the rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains

Homologues:

Organism=Homo sapiens, GI66933016, Length=495, Percent_Identity=37.5757575757576, Blast_Score=296, Evalue=4e-80,
Organism=Homo sapiens, GI217035146, Length=495, Percent_Identity=36.969696969697, Blast_Score=287, Evalue=2e-77,
Organism=Homo sapiens, GI34328928, Length=495, Percent_Identity=36.969696969697, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI34328930, Length=495, Percent_Identity=36.969696969697, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI156616279, Length=495, Percent_Identity=36.969696969697, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI217035152, Length=483, Percent_Identity=37.4741200828157, Blast_Score=280, Evalue=3e-75,
Organism=Homo sapiens, GI217035148, Length=495, Percent_Identity=36.5656565656566, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI217035150, Length=495, Percent_Identity=34.5454545454545, Blast_Score=251, Evalue=2e-66,
Organism=Homo sapiens, GI156104880, Length=257, Percent_Identity=40.8560311284047, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI50541954, Length=256, Percent_Identity=40.625, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI50541952, Length=256, Percent_Identity=40.625, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI50541948, Length=256, Percent_Identity=40.625, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI50541956, Length=256, Percent_Identity=40.625, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1788855, Length=492, Percent_Identity=49.5934959349593, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1786293, Length=346, Percent_Identity=34.1040462427746, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71994385, Length=513, Percent_Identity=32.943469785575, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI71994389, Length=434, Percent_Identity=35.0230414746544, Blast_Score=225, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17560440, Length=286, Percent_Identity=36.7132867132867, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323464, Length=498, Percent_Identity=38.3534136546185, Blast_Score=311, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6322012, Length=498, Percent_Identity=37.5502008032129, Blast_Score=309, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6323585, Length=485, Percent_Identity=37.9381443298969, Blast_Score=302, Evalue=7e-83,
Organism=Saccharomyces cerevisiae, GI6319352, Length=336, Percent_Identity=37.797619047619, Blast_Score=226, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6319353, Length=138, Percent_Identity=39.8550724637681, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24641071, Length=495, Percent_Identity=37.7777777777778, Blast_Score=304, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24641073, Length=495, Percent_Identity=37.7777777777778, Blast_Score=304, Evalue=8e-83,
Organism=Drosophila melanogaster, GI28571163, Length=446, Percent_Identity=38.7892376681614, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): IMDH_METJA (Q59011)

Other databases:

- EMBL:   L77117
- PIR:   G64501
- RefSeq:   NP_248626.1
- ProteinModelPortal:   Q59011
- SMR:   Q59011
- GeneID:   1452525
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1616
- NMPDR:   fig|243232.1.peg.1663
- TIGR:   MJ1616
- HOGENOM:   HBG298985
- OMA:   INGRKYK
- ProtClustDB:   CLSK876645
- BioCyc:   MJAN243232:MJ_1616-MONOMER
- BRENDA:   1.1.1.205
- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR005990
- InterPro:   IPR018529
- InterPro:   IPR015875
- InterPro:   IPR001093
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000130
- SMART:   SM00116
- TIGRFAMs:   TIGR01302

Pfam domain/function: PF00571 CBS; PF00478 IMPDH

EC number: =1.1.1.205

Molecular weight: Translated: 53317; Mature: 53317

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS51371 CBS; PS00487 IMP_DH_GMP_RED

Important sites: ACT_SITE 306-306 BINDING 339-339 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLKKLIEAKKAYTFDDVLLVPNASWVEPKDTDVSTDLAGLKLNIPIVSAAMDTVTEKEM
CHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
AIALARLGGLGVIHRNMSIEEQVHQVQAVKKADEVVIKDVITVSPDDTVGEAINVMETYS
HHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCC
ISGLPVVDNEDKLVGIITHRDVKAIEDKTKKVKDVMTKDVVCAKEDVEEEEALELMYANR
CCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHH
VERLPIVDDENRLIGIITLRDILKRRKYPQAARDKKGRLLVAAACGPHDFERAKALIEAE
HCCCCCCCCCCCEEEEEEHHHHHHHCCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHC
VDAIAIDCAHAHNMRVVENVKKFKEMLEGTDIKLIVGNIATKEAAEDLIKAGADVLKVGI
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECC
GPGSICTTRVVAGVGVPQLTAVAEVADVAKEHNVPIIADGGIRYSGDIAKAIAAGADAVM
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEECCHHHHHHHCCCCHHH
LGSLLAGTDEAPGQLMVINGRKYKQYRGMGSLGAMTGGVGAGADRYFQAPAKSHMKHVKL
HHHHHCCCCCCCCEEEEECCCHHHHHCCCCCHHHCCCCCCCCCHHHHCCCHHHHHHEEEE
VPEGVEGAVPYKGPVSEVVFQLIGGLRASMGYCGAKNLKEMQEKARFVIITPSGQVESHP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCC
HDIIITNEAPNYPLGK
CCEEEECCCCCCCCCC
>Mature Secondary Structure
MFLKKLIEAKKAYTFDDVLLVPNASWVEPKDTDVSTDLAGLKLNIPIVSAAMDTVTEKEM
CHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
AIALARLGGLGVIHRNMSIEEQVHQVQAVKKADEVVIKDVITVSPDDTVGEAINVMETYS
HHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCC
ISGLPVVDNEDKLVGIITHRDVKAIEDKTKKVKDVMTKDVVCAKEDVEEEEALELMYANR
CCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHH
VERLPIVDDENRLIGIITLRDILKRRKYPQAARDKKGRLLVAAACGPHDFERAKALIEAE
HCCCCCCCCCCCEEEEEEHHHHHHHCCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHHC
VDAIAIDCAHAHNMRVVENVKKFKEMLEGTDIKLIVGNIATKEAAEDLIKAGADVLKVGI
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECC
GPGSICTTRVVAGVGVPQLTAVAEVADVAKEHNVPIIADGGIRYSGDIAKAIAAGADAVM
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEECCHHHHHHHCCCCHHH
LGSLLAGTDEAPGQLMVINGRKYKQYRGMGSLGAMTGGVGAGADRYFQAPAKSHMKHVKL
HHHHHCCCCCCCCEEEEECCCHHHHHCCCCCHHHCCCCCCCCCHHHHCCCHHHHHHEEEE
VPEGVEGAVPYKGPVSEVVFQLIGGLRASMGYCGAKNLKEMQEKARFVIITPSGQVESHP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCC
HDIIITNEAPNYPLGK
CCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087