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The map label for this gene is aspC

Identifier: 15669750

GI number: 15669750

Start: 1531340

End: 1532656

Strand: Direct

Name: aspC

Synonym: MJ1555

Alternate gene names: 15669750

Gene position: 1531340-1532656 (Clockwise)

Preceding gene: 15669749

Following gene: 15669751

Centisome position: 91.97

GC content: 35.54

Gene sequence:

>1317_bases
ATGGTGAATAAAATGAAGTGGAGAAGAACACACTACTCAGCAGATATTAAACCAGAAATGGATGGACAAGAAGTTATTAT
AATGGGATGGGTTCATTCAATTAGAGCTTTAGGGAAAATTATATTTGTTATTTTAAGAGATAGAGAAGGGACAGTGCAAA
TTGTAGCACCAAAACAAAAAGTTGGAGATGAGTTGTTTAGCCAAATAAAAAAACTTGGAGCAGAGGATGTTATAGCAGTT
AAAGGAAAAGTTATAGCAAATGAAAAAGCACCAAATGGTTTTGAAATATTACCATTAGAGTTGGAGGTTATAAACACCGC
TAAAAGACCTCTTCCTTTAGACCCAGCTGAAAAAGTCCCAGCAGAGTTAGATACAAGATTAGAAAATAGATTCTTAGATT
TAAGAAGACCAAAAGTTCAGGCAATATTTAAAATTAGAAGTGAAATGCTAAAATCTGTAAGAAACACACTCTATAATGAG
GGCTTTATTGAGGTAAATACACCAAAATTGGTAGCAAGTTGCACTGAGGGAGGAACTGAACTCTTCCCAATCTCATACTT
TGAGAGAGAGGCATTTTTAGGGCAGAGTCCTCAGTTGTATAAGCAGATGTTAATGGCAACTGGGTTGGATAGAGTTTTTG
AAATAGCTCCAATATTTAGGGCTGAGGAGCATAATACAAGGAGACATTTAAATGAAGCTACATCAATAGACATTGAAATG
GCATTTGCTGATGATAAGGATGCTATGGATATATTGGAGAAAGTTGTTTATAACGCATTTGTAGATGTTTATGAGAATAG
AAAGAAGGAGATTGAAACTTTAGGAATAGAGTTTGAGTTGCCACCAGAGAAGTTTGATAGAATCACTTACGATGAAGCTA
TTGACATTGCAAATGCAAAAGGTGTTGAAATTAGCTGGGGAGAAGATTTGAGTAGAGAGGCAGAGAAGGCTATTGGAGAG
GAGATGGAAGGACTCTACTTTATAACTGACTGGCCTTCTGAAATTAGGCCGTTCTATACAATGCCAGATGAGAAAAATCC
AAATATTTGTAAGGCATTTGATTTAATGTATAAAGATTTAGAGATTTCTTCAGGAGCTCAAAGGATTCACTTATATGATT
TATTAGTTGAGAATATTAAAAAGAAGGGCTTAAACCCTGATGGATTTACATATTACTTAGAGGCATTTAAGTATGGAATG
CCTCCACATGCAGGATGGGGATTAGGAGCGGATAGATTTACCATGGTATTAACTCAGCAAGAGAACATTAGAGAGTGTGT
TTTATTCCCAAGAGATAGGCAGAGATTAACTCCATAA

Upstream 100 bases:

>100_bases
TAAATATATAGATACACAAAAGCTTAGGAAACTATAAAAACCATTTACACAATTATTAACCTTAAAAAGTTTTTAGTGAT
TTGGGAAACACATGATGTAG

Downstream 100 bases:

>100_bases
TTTTTAAATACTTTTTAATGAGAAATTAATAAGATTATTATGATGGATAAGATAATTCAAACACTTACATTGACATTTAC
TTTTTTGTATTACAGCATTC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVNKMKWRRTHYSADIKPEMDGQEVIIMGWVHSIRALGKIIFVILRDREGTVQIVAPKQKVGDELFSQIKKLGAEDVIAV
KGKVIANEKAPNGFEILPLELEVINTAKRPLPLDPAEKVPAELDTRLENRFLDLRRPKVQAIFKIRSEMLKSVRNTLYNE
GFIEVNTPKLVASCTEGGTELFPISYFEREAFLGQSPQLYKQMLMATGLDRVFEIAPIFRAEEHNTRRHLNEATSIDIEM
AFADDKDAMDILEKVVYNAFVDVYENRKKEIETLGIEFELPPEKFDRITYDEAIDIANAKGVEISWGEDLSREAEKAIGE
EMEGLYFITDWPSEIRPFYTMPDEKNPNICKAFDLMYKDLEISSGAQRIHLYDLLVENIKKKGLNPDGFTYYLEAFKYGM
PPHAGWGLGADRFTMVLTQQENIRECVLFPRDRQRLTP

Sequences:

>Translated_438_residues
MVNKMKWRRTHYSADIKPEMDGQEVIIMGWVHSIRALGKIIFVILRDREGTVQIVAPKQKVGDELFSQIKKLGAEDVIAV
KGKVIANEKAPNGFEILPLELEVINTAKRPLPLDPAEKVPAELDTRLENRFLDLRRPKVQAIFKIRSEMLKSVRNTLYNE
GFIEVNTPKLVASCTEGGTELFPISYFEREAFLGQSPQLYKQMLMATGLDRVFEIAPIFRAEEHNTRRHLNEATSIDIEM
AFADDKDAMDILEKVVYNAFVDVYENRKKEIETLGIEFELPPEKFDRITYDEAIDIANAKGVEISWGEDLSREAEKAIGE
EMEGLYFITDWPSEIRPFYTMPDEKNPNICKAFDLMYKDLEISSGAQRIHLYDLLVENIKKKGLNPDGFTYYLEAFKYGM
PPHAGWGLGADRFTMVLTQQENIRECVLFPRDRQRLTP
>Mature_438_residues
MVNKMKWRRTHYSADIKPEMDGQEVIIMGWVHSIRALGKIIFVILRDREGTVQIVAPKQKVGDELFSQIKKLGAEDVIAV
KGKVIANEKAPNGFEILPLELEVINTAKRPLPLDPAEKVPAELDTRLENRFLDLRRPKVQAIFKIRSEMLKSVRNTLYNE
GFIEVNTPKLVASCTEGGTELFPISYFEREAFLGQSPQLYKQMLMATGLDRVFEIAPIFRAEEHNTRRHLNEATSIDIEM
AFADDKDAMDILEKVVYNAFVDVYENRKKEIETLGIEFELPPEKFDRITYDEAIDIANAKGVEISWGEDLSREAEKAIGE
EMEGLYFITDWPSEIRPFYTMPDEKNPNICKAFDLMYKDLEISSGAQRIHLYDLLVENIKKKGLNPDGFTYYLEAFKYGM
PPHAGWGLGADRFTMVLTQQENIRECVLFPRDRQRLTP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI45439306, Length=445, Percent_Identity=37.5280898876404, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI4758762, Length=434, Percent_Identity=31.7972350230415, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI226491502, Length=443, Percent_Identity=26.6365688487585, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI194272210, Length=456, Percent_Identity=28.9473684210526, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI5031815, Length=456, Percent_Identity=28.9473684210526, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI40789249, Length=262, Percent_Identity=35.1145038167939, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1789256, Length=470, Percent_Identity=28.5106382978723, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1790571, Length=470, Percent_Identity=28.5106382978723, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1787161, Length=445, Percent_Identity=26.9662921348315, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788173, Length=262, Percent_Identity=34.7328244274809, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17551876, Length=450, Percent_Identity=34.6666666666667, Blast_Score=281, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI71984122, Length=432, Percent_Identity=32.4074074074074, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17556372, Length=406, Percent_Identity=27.8325123152709, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71994340, Length=475, Percent_Identity=25.8947368421053, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17535925, Length=475, Percent_Identity=26.1052631578947, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17535927, Length=475, Percent_Identity=25.8947368421053, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566633, Length=296, Percent_Identity=31.4189189189189, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323011, Length=451, Percent_Identity=37.4722838137472, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6321807, Length=433, Percent_Identity=32.7944572748268, Blast_Score=225, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6319872, Length=464, Percent_Identity=28.448275862069, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6325153, Length=296, Percent_Identity=32.7702702702703, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6320242, Length=462, Percent_Identity=25.1082251082251, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17136276, Length=459, Percent_Identity=37.2549019607843, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI19921528, Length=435, Percent_Identity=29.4252873563218, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI19922416, Length=460, Percent_Identity=25.4347826086956, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24640851, Length=463, Percent_Identity=27.645788336933, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24640849, Length=463, Percent_Identity=27.645788336933, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24584738, Length=297, Percent_Identity=31.986531986532, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_METJA (Q58950)

Other databases:

- EMBL:   L77117
- PIR:   B64494
- RefSeq:   NP_248563.1
- ProteinModelPortal:   Q58950
- SMR:   Q58950
- GeneID:   1452463
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1555
- NMPDR:   fig|243232.1.peg.1600
- TIGR:   MJ1555
- HOGENOM:   HBG745843
- OMA:   TDWPSEI
- ProtClustDB:   PRK05159
- BioCyc:   MJAN243232:MJ_1555-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_A
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00458

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 50351; Mature: 50351

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNKMKWRRTHYSADIKPEMDGQEVIIMGWVHSIRALGKIIFVILRDREGTVQIVAPKQK
CCCCCCHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHEEEEEECCCCEEEEEECHHH
VGDELFSQIKKLGAEDVIAVKGKVIANEKAPNGFEILPLELEVINTAKRPLPLDPAEKVP
HHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHCC
AELDTRLENRFLDLRRPKVQAIFKIRSEMLKSVRNTLYNEGFIEVNTPKLVASCTEGGTE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHEEECCCCCCE
LFPISYFEREAFLGQSPQLYKQMLMATGLDRVFEIAPIFRAEEHNTRRHLNEATSIDIEM
EEEEHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEE
AFADDKDAMDILEKVVYNAFVDVYENRKKEIETLGIEFELPPEKFDRITYDEAIDIANAK
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCHHHHHCCCCCC
GVEISWGEDLSREAEKAIGEEMEGLYFITDWPSEIRPFYTMPDEKNPNICKAFDLMYKDL
CCEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHCCCCEECCCCCCCCHHHHHHHHHHHC
EISSGAQRIHLYDLLVENIKKKGLNPDGFTYYLEAFKYGMPPHAGWGLGADRFTMVLTQQ
CCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
ENIRECVLFPRDRQRLTP
CCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVNKMKWRRTHYSADIKPEMDGQEVIIMGWVHSIRALGKIIFVILRDREGTVQIVAPKQK
CCCCCCHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHEEEEEECCCCEEEEEECHHH
VGDELFSQIKKLGAEDVIAVKGKVIANEKAPNGFEILPLELEVINTAKRPLPLDPAEKVP
HHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHCC
AELDTRLENRFLDLRRPKVQAIFKIRSEMLKSVRNTLYNEGFIEVNTPKLVASCTEGGTE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHEEECCCCCCE
LFPISYFEREAFLGQSPQLYKQMLMATGLDRVFEIAPIFRAEEHNTRRHLNEATSIDIEM
EEEEHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEE
AFADDKDAMDILEKVVYNAFVDVYENRKKEIETLGIEFELPPEKFDRITYDEAIDIANAK
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCHHHHHCCCCCC
GVEISWGEDLSREAEKAIGEEMEGLYFITDWPSEIRPFYTMPDEKNPNICKAFDLMYKDL
CCEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHCCCCEECCCCCCCCHHHHHHHHHHHC
EISSGAQRIHLYDLLVENIKKKGLNPDGFTYYLEAFKYGMPPHAGWGLGADRFTMVLTQQ
CCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECC
ENIRECVLFPRDRQRLTP
CCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087