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The map label for this gene is hisA

Identifier: 15669727

GI number: 15669727

Start: 1508877

End: 1509590

Strand: Direct

Name: hisA

Synonym: MJ1532

Alternate gene names: 15669727

Gene position: 1508877-1509590 (Clockwise)

Preceding gene: 15669726

Following gene: 15669729

Centisome position: 90.62

GC content: 30.11

Gene sequence:

>714_bases
ATGATAATAATCCCTGCAGTTGATTTAAAAGATAAAAAGTGCGTTCAGCTAATTCAAGGAGACCCAAATAAAAAGCATTT
GGAACTAAACAATCCAGTAGAAGTTGCTAAAAAATTTGTAGATGAAGGGGCTGAGTATCTACATATAATCGATTTAGATG
CCGCATTTGGAACAGGAAATAATAGAGACGTTATTAAGAACATTATAAAAGAGGTTAATGTTCCAGTTGAGGTTGGAGGA
GGAATTAGAAATTTAGAGATAGCAAAGGAATTAATTAGCTTGGGAGTTGATAGAGTTATAGTGGGAACTAAGGCAATTTT
AGAACCAAAATTCATAGATGATTTAAATAAAGAGATTGGAAAAGATAAGATAGTTTTAGCTGTAGAATGTAAAGAGGGAA
AGGTTGTTATTAAAGGATGGAAGGAAAAAGTAGATAAAACTCCAATAGAAGTTATTAAGGAATTTGAAGATAAGGTTGGC
TATATATTATTTACAAATGTAGATGTTGAGGGCTTATTAAAAGGAATAAATGTTGATATAATTAAAGAGTTAATTGAAAA
GACTGATATTCCTATCATCTACTCTGGTGGAATTACAACCTTAGAAGACATAAAAGCTTTAAAAGAACTTGGTATTTATG
GAGTTGTTATTGGTTCAGCACTATATAAAGGTTTAATTGATTTAAAAAAGGCGTTAGAGATAGTTAAAAATTAA

Upstream 100 bases:

>100_bases
ATAGAAAAAGCAAAAAAGAAAATAATGAATCTACTACTAACTGGAAAAATAAGAGTAAAAACCTAAACAATATCATTACT
TATTCATATAGGTGATTTAA

Downstream 100 bases:

>100_bases
GATAGGATTTTTTATTGCAAATCAACATATATGTCTCTTCCTTCATCAATTGCTTCTAAAATCTCTTTTCCTACCTTTGT
TTTTTTGTTTATCCTCACTG

Product: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase HisA1

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MIIIPAVDLKDKKCVQLIQGDPNKKHLELNNPVEVAKKFVDEGAEYLHIIDLDAAFGTGNNRDVIKNIIKEVNVPVEVGG
GIRNLEIAKELISLGVDRVIVGTKAILEPKFIDDLNKEIGKDKIVLAVECKEGKVVIKGWKEKVDKTPIEVIKEFEDKVG
YILFTNVDVEGLLKGINVDIIKELIEKTDIPIIYSGGITTLEDIKALKELGIYGVVIGSALYKGLIDLKKALEIVKN

Sequences:

>Translated_237_residues
MIIIPAVDLKDKKCVQLIQGDPNKKHLELNNPVEVAKKFVDEGAEYLHIIDLDAAFGTGNNRDVIKNIIKEVNVPVEVGG
GIRNLEIAKELISLGVDRVIVGTKAILEPKFIDDLNKEIGKDKIVLAVECKEGKVVIKGWKEKVDKTPIEVIKEFEDKVG
YILFTNVDVEGLLKGINVDIIKELIEKTDIPIIYSGGITTLEDIKALKELGIYGVVIGSALYKGLIDLKKALEIVKN
>Mature_237_residues
MIIIPAVDLKDKKCVQLIQGDPNKKHLELNNPVEVAKKFVDEGAEYLHIIDLDAAFGTGNNRDVIKNIIKEVNVPVEVGG
GIRNLEIAKELISLGVDRVIVGTKAILEPKFIDDLNKEIGKDKIVLAVECKEGKVVIKGWKEKVDKTPIEVIKEFEDKVG
YILFTNVDVEGLLKGINVDIIKELIEKTDIPIIYSGGITTLEDIKALKELGIYGVVIGSALYKGLIDLKKALEIVKN

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=33.8775510204082, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1788336, Length=239, Percent_Identity=24.2677824267782, Blast_Score=86, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322169, Length=242, Percent_Identity=25.2066115702479, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_METJA (Q58927)

Other databases:

- EMBL:   L77117
- PIR:   C64491
- RefSeq:   NP_248540.1
- ProteinModelPortal:   Q58927
- SMR:   Q58927
- GeneID:   1452440
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1532
- NMPDR:   fig|243232.1.peg.1577
- TIGR:   MJ1532
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   CLSK2517743
- BioCyc:   MJAN243232:MJ_1532-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26132; Mature: 26132

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIIPAVDLKDKKCVQLIQGDPNKKHLELNNPVEVAKKFVDEGAEYLHIIDLDAAFGTGN
CEEECCCCCCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCC
NRDVIKNIIKEVNVPVEVGGGIRNLEIAKELISLGVDRVIVGTKAILEPKFIDDLNKEIG
CHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCEEECHHHHCCCHHHHHHHHHHC
KDKIVLAVECKEGKVVIKGWKEKVDKTPIEVIKEFEDKVGYILFTNVDVEGLLKGINVDI
CCEEEEEEEECCCCEEEECHHHHHCCCHHHHHHHHHHCCCEEEEECCCHHHHHHCCCHHH
IKELIEKTDIPIIYSGGITTLEDIKALKELGIYGVVIGSALYKGLIDLKKALEIVKN
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIIIPAVDLKDKKCVQLIQGDPNKKHLELNNPVEVAKKFVDEGAEYLHIIDLDAAFGTGN
CEEECCCCCCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCC
NRDVIKNIIKEVNVPVEVGGGIRNLEIAKELISLGVDRVIVGTKAILEPKFIDDLNKEIG
CHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCEEECHHHHCCCHHHHHHHHHHC
KDKIVLAVECKEGKVVIKGWKEKVDKTPIEVIKEFEDKVGYILFTNVDVEGLLKGINVDI
CCEEEEEEEECCCCEEEECHHHHHCCCHHHHHHHHHHCCCEEEEECCCHHHHHHCCCHHH
IKELIEKTDIPIIYSGGITTLEDIKALKELGIYGVVIGSALYKGLIDLKKALEIVKN
HHHHHHHCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087