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The map label for this gene is mjaVM

Identifier: 15669692

GI number: 15669692

Start: 1470766

End: 1471644

Strand: Direct

Name: mjaVM

Synonym: MJ1498

Alternate gene names: 15669692

Gene position: 1470766-1471644 (Clockwise)

Preceding gene: 15669691

Following gene: 15669701

Centisome position: 88.34

GC content: 30.15

Gene sequence:

>879_bases
ATGGAGATAAATAAAATCTACTGTATGGATTGTTTAGAGGGAATGAAACAGTTAAAAGATAAGACAGTTGATGTTGTTGT
TACGTCTCCACCCTATAATATTGGCATAAAATACAACAAATACTCTGATAATTTAAGTAGAGAAGATTATTTAAATTGGA
TTGAAGAAGTTGTCAAAGAAATAAAGAGAGTATTAAAAGATGACGGCTCATTTTTTATAAATGTTGGCTATACTGCAAAA
GACCCGTGGATTGCCTTTGATGTTGCTAATGTTATAAGAAAACATTTTAAATTACAAAATACTATACATTGGGTAAAATC
AATTGCAATACAAAAAGAAGATGTTGGAAATTACCCAAATATTATTGGAGATATTGCTGTTGGACATTACAAGCCAATAA
ACAGTGATAGATTTTTAAGTATAATGCATGAATATATTTTCCACTTCACAAAAAATGGGAACGTTAAGTTAGACAAATTA
GCAATTGGTGTTCCTTATCAGGATAAAAGCAATATAAAGAGATTTAACAGAAAAGGAGATTTAAGAGATAGGGGAAATAC
GTGGTTTATCCCTTATGAAACAATCCAATCAAAAGAAAAAGAAAGACCTCATCCTGCAACATTTCCTCCAAAACTTCCGG
AGATGTGTATTAAATTGCATGGTGTTAAAAAGACAAATCTTGTTTTAGACCCATTTATGGGAATTGGAAGCACAGCAATA
GCATGTATTAGGTTAGGGATTGATTATATTGGCTTTGAGATTGATGAATACTATTGCAGAGTTGCAGAGGAGAGGATAAA
AAAAGAACTTTTAAAAACAGATGGAAAATTTGATAATGTAAAAAACAAAAACATAATTACTCTTGATGCATTTATTTAA

Upstream 100 bases:

>100_bases
AAAGAAGAATATTTAAACTTAAATGATTTTGATAGGGGAATTAAAGAAGTAGAGAAGCTTGCAGAATTGATGATAGAATA
AATAATGATGGGGATTAAAA

Downstream 100 bases:

>100_bases
ATAGTGTTCTTAAGATTGTGAACAGTCGTTTTTAGAGAATTTGCTAAATTTTTTATATTTTGAACTCCTGTCGCAGAACC
TTCTAAATTTTCTAATTCAC

Product: type II R/M system modification methyltransferase

Products: NA

Alternate protein names: M.MjaV; N-4 cytosine-specific methyltransferase MjaV

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MEINKIYCMDCLEGMKQLKDKTVDVVVTSPPYNIGIKYNKYSDNLSREDYLNWIEEVVKEIKRVLKDDGSFFINVGYTAK
DPWIAFDVANVIRKHFKLQNTIHWVKSIAIQKEDVGNYPNIIGDIAVGHYKPINSDRFLSIMHEYIFHFTKNGNVKLDKL
AIGVPYQDKSNIKRFNRKGDLRDRGNTWFIPYETIQSKEKERPHPATFPPKLPEMCIKLHGVKKTNLVLDPFMGIGSTAI
ACIRLGIDYIGFEIDEYYCRVAEERIKKELLKTDGKFDNVKNKNIITLDAFI

Sequences:

>Translated_292_residues
MEINKIYCMDCLEGMKQLKDKTVDVVVTSPPYNIGIKYNKYSDNLSREDYLNWIEEVVKEIKRVLKDDGSFFINVGYTAK
DPWIAFDVANVIRKHFKLQNTIHWVKSIAIQKEDVGNYPNIIGDIAVGHYKPINSDRFLSIMHEYIFHFTKNGNVKLDKL
AIGVPYQDKSNIKRFNRKGDLRDRGNTWFIPYETIQSKEKERPHPATFPPKLPEMCIKLHGVKKTNLVLDPFMGIGSTAI
ACIRLGIDYIGFEIDEYYCRVAEERIKKELLKTDGKFDNVKNKNIITLDAFI
>Mature_292_residues
MEINKIYCMDCLEGMKQLKDKTVDVVVTSPPYNIGIKYNKYSDNLSREDYLNWIEEVVKEIKRVLKDDGSFFINVGYTAK
DPWIAFDVANVIRKHFKLQNTIHWVKSIAIQKEDVGNYPNIIGDIAVGHYKPINSDRFLSIMHEYIFHFTKNGNVKLDKL
AIGVPYQDKSNIKRFNRKGDLRDRGNTWFIPYETIQSKEKERPHPATFPPKLPEMCIKLHGVKKTNLVLDPFMGIGSTAI
ACIRLGIDYIGFEIDEYYCRVAEERIKKELLKTDGKFDNVKNKNIITLDAFI

Specific function: This methylase recognizes the double-stranded sequence GTAC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the MjaV endonuclease (Potential)

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily

Homologues:

Organism=Escherichia coli, GI87082238, Length=272, Percent_Identity=27.5735294117647, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTM5_METJA (Q58893)

Other databases:

- EMBL:   L77117
- PIR:   A64487
- RefSeq:   NP_248505.1
- ProteinModelPortal:   Q58893
- SMR:   Q58893
- REBASE:   3897
- GeneID:   1452405
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1498
- NMPDR:   fig|243232.1.peg.1542
- TIGR:   MJ1498
- HOGENOM:   HBG493024
- OMA:   DIAVGHY
- ProtClustDB:   CLSK876599
- BioCyc:   MJAN243232:MJ_1498-MONOMER
- BRENDA:   2.1.1.113
- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091
- PRINTS:   PR00508

Pfam domain/function: PF01555 N6_N4_Mtase

EC number: =2.1.1.113

Molecular weight: Translated: 33878; Mature: 33878

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINKIYCMDCLEGMKQLKDKTVDVVVTSPPYNIGIKYNKYSDNLSREDYLNWIEEVVKE
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
IKRVLKDDGSFFINVGYTAKDPWIAFDVANVIRKHFKLQNTIHWVKSIAIQKEDVGNYPN
HHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IIGDIAVGHYKPINSDRFLSIMHEYIFHFTKNGNVKLDKLAIGVPYQDKSNIKRFNRKGD
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCC
LRDRGNTWFIPYETIQSKEKERPHPATFPPKLPEMCIKLHGVKKTNLVLDPFMGIGSTAI
CCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCCCHHHH
ACIRLGIDYIGFEIDEYYCRVAEERIKKELLKTDGKFDNVKNKNIITLDAFI
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MEINKIYCMDCLEGMKQLKDKTVDVVVTSPPYNIGIKYNKYSDNLSREDYLNWIEEVVKE
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
IKRVLKDDGSFFINVGYTAKDPWIAFDVANVIRKHFKLQNTIHWVKSIAIQKEDVGNYPN
HHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IIGDIAVGHYKPINSDRFLSIMHEYIFHFTKNGNVKLDKLAIGVPYQDKSNIKRFNRKGD
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCC
LRDRGNTWFIPYETIQSKEKERPHPATFPPKLPEMCIKLHGVKKTNLVLDPFMGIGSTAI
CCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCCCHHHH
ACIRLGIDYIGFEIDEYYCRVAEERIKKELLKTDGKFDNVKNKNIITLDAFI
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087