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The map label for this gene is lasT [C]

Identifier: 15669668

GI number: 15669668

Start: 1446816

End: 1447508

Strand: Direct

Name: lasT [C]

Synonym: MJ1476

Alternate gene names: 15669668

Gene position: 1446816-1447508 (Clockwise)

Preceding gene: 15669667

Following gene: 15669669

Centisome position: 86.9

GC content: 31.89

Gene sequence:

>693_bases
GTGATAATAATGATTTCTGTCATCTTAGTTAATCCAAAATACAGTGGAAATGTTGGTAGTATAGCAAGGGTTATGATGAA
TTTTGGATTTGAAGAGCTTAGAATAGTTGGAGATAAAAGCATAATAAATAATGAAGCCTATATGATGGCAGTCCATGCAA
GAGAGATTTTAGACAATGCCAAATTCTACAACACCTTTGATGAAGCTATAGGGGATTTAGATTTTGTTATTGCCACTTCA
GGAGCAAGAGGAGGAGATAGAAATTTAAAGAGAGTTCCAATAACACCAAAAGAGTTGGCAGATAAAATCTTAGAGGTTAA
GGGAAACATTGGGATTGTCTTTGGTAGGGAAGATGATGGATTGAGAAATGAAGAGATAGATAAATGTGATTTGTTAGTTT
CAATACCAACATCTGAAAAGTATCCAATTATGAACCTATCTCATGCTGTTGCAGTTATTTTATATGAGATTTATACAAAA
AAAGTTAGAAATAAATTTTTAGATATAAATATGAGAGAGGCATCAAAAGAGGACAAAGAGCTATTGATAAGGAAATTTAA
TGAGTTTATTGACAAGAATGAAAAAATCCCAGAGCATAAAAAAGAACTCTGCAAAATAATCTTTAAGAGATTGGTTAATA
GGGCTTTTATAAGTGGAAAGGAGGCATGGACGTTAATGAGTGCATTTAAATAA

Upstream 100 bases:

>100_bases
AGTATTTAGATAAAAAACCTAAAGAAATAGCTGAAATTACTGGGATTAATATAAAAACCGTTGAGTACCATTATTATAAG
CTGAAAAAGAAGGAAAAACA

Downstream 100 bases:

>100_bases
AATTAGGGGAAAGAACATGAAGAAAAGCCATATTTTAGGAATAATAATTTGCATTATTTTAATTGTAGGATTTTTTATTT
CATTTGATAGTACTTTTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIIMISVILVNPKYSGNVGSIARVMMNFGFEELRIVGDKSIINNEAYMMAVHAREILDNAKFYNTFDEAIGDLDFVIATS
GARGGDRNLKRVPITPKELADKILEVKGNIGIVFGREDDGLRNEEIDKCDLLVSIPTSEKYPIMNLSHAVAVILYEIYTK
KVRNKFLDINMREASKEDKELLIRKFNEFIDKNEKIPEHKKELCKIIFKRLVNRAFISGKEAWTLMSAFK

Sequences:

>Translated_230_residues
MIIMISVILVNPKYSGNVGSIARVMMNFGFEELRIVGDKSIINNEAYMMAVHAREILDNAKFYNTFDEAIGDLDFVIATS
GARGGDRNLKRVPITPKELADKILEVKGNIGIVFGREDDGLRNEEIDKCDLLVSIPTSEKYPIMNLSHAVAVILYEIYTK
KVRNKFLDINMREASKEDKELLIRKFNEFIDKNEKIPEHKKELCKIIFKRLVNRAFISGKEAWTLMSAFK
>Mature_230_residues
MIIMISVILVNPKYSGNVGSIARVMMNFGFEELRIVGDKSIINNEAYMMAVHAREILDNAKFYNTFDEAIGDLDFVIATS
GARGGDRNLKRVPITPKELADKILEVKGNIGIVFGREDDGLRNEEIDKCDLLVSIPTSEKYPIMNLSHAVAVILYEIYTK
KVRNKFLDINMREASKEDKELLIRKFNEFIDKNEKIPEHKKELCKIIFKRLVNRAFISGKEAWTLMSAFK

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family

Homologues:

Organism=Escherichia coli, GI1790865, Length=155, Percent_Identity=34.1935483870968, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788881, Length=159, Percent_Identity=35.8490566037736, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1476_METJA (Q58871)

Other databases:

- EMBL:   L77117
- PIR:   C64484
- RefSeq:   NP_248481.1
- ProteinModelPortal:   Q58871
- GeneID:   1452381
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1476
- NMPDR:   fig|243232.1.peg.1518
- TIGR:   MJ1476
- HOGENOM:   HBG724367
- OMA:   AFISGKE
- ProtClustDB:   CLSK876589
- BioCyc:   MJAN243232:MJ_1476-MONOMER
- InterPro:   IPR004384
- InterPro:   IPR001537
- PIRSF:   PIRSF004808
- TIGRFAMs:   TIGR00050

Pfam domain/function: PF00588 SpoU_methylase

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26280; Mature: 26280

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIMISVILVNPKYSGNVGSIARVMMNFGFEELRIVGDKSIINNEAYMMAVHAREILDNA
CEEEEEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCHHHCCCCCEEEHHHHHHHHHCC
KFYNTFDEAIGDLDFVIATSGARGGDRNLKRVPITPKELADKILEVKGNIGIVFGREDDG
HHHHHHHHHHCCEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHCCCEEEEECCCCCC
LRNEEIDKCDLLVSIPTSEKYPIMNLSHAVAVILYEIYTKKVRNKFLDINMREASKEDKE
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHH
LLIRKFNEFIDKNEKIPEHKKELCKIIFKRLVNRAFISGKEAWTLMSAFK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MIIMISVILVNPKYSGNVGSIARVMMNFGFEELRIVGDKSIINNEAYMMAVHAREILDNA
CEEEEEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCHHHCCCCCEEEHHHHHHHHHCC
KFYNTFDEAIGDLDFVIATSGARGGDRNLKRVPITPKELADKILEVKGNIGIVFGREDDG
HHHHHHHHHHCCEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHCCCEEEEECCCCCC
LRNEEIDKCDLLVSIPTSEKYPIMNLSHAVAVILYEIYTKKVRNKFLDINMREASKEDKE
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHH
LLIRKFNEFIDKNEKIPEHKKELCKIIFKRLVNRAFISGKEAWTLMSAFK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087