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The map label for this gene is cimA

Identifier: 15669582

GI number: 15669582

Start: 1340251

End: 1341726

Strand: Direct

Name: cimA

Synonym: MJ1392

Alternate gene names: 15669582

Gene position: 1340251-1341726 (Clockwise)

Preceding gene: 15669578

Following gene: 15669583

Centisome position: 80.5

GC content: 34.42

Gene sequence:

>1476_bases
ATGATGGTAAGGATATTTGATACAACACTTAGAGATGGAGAGCAAACACCAGGAGTTTCTTTAACACCAAATGATAAGTT
AGAGATAGCAAAAAAATTGGATGAGCTTGGAGTTGATGTTATAGAGGCAGGTTCAGCTATAACTTCAAAAGGAGAGAGAG
AAGGAATAAAATTAATAACAAAAGAAGGTTTAAATGCAGAAATCTGCTCATTTGTTAGAGCTTTACCTGTAGATATTGAT
GCTGCCTTAGAATGTGATGTAGATAGTGTCCATTTAGTAGTGCCAACATCTCCAATACACATGAAATATAAGCTTAGAAA
AACAGAAGATGAGGTTTTAGAGACAGCTTTAAAGGCTGTAGAGTATGCTAAAGAACATGGATTGATTGTTGAGTTATCTG
CAGAGGATGCAACAAGAAGTGATGTAAATTTCTTAATAAAACTATTTAATGAAGGGGAAAAGGTTGGAGCAGACAGAGTT
TGTGTTTGTGACACAGTAGGAGTTTTAACTCCACAAAAGAGTCAGGAATTATTTAAAAAAATAACTGAAAATGTTAATTT
ACCGGTCTCAGTTCATTGCCACAACGACTTTGGAATGGCTACTGCTAATACTTGCTCAGCAGTTTTAGGTGGAGCTGTTC
AGTGCCACGTAACAGTTAATGGTATTGGAGAGAGAGCAGGAAATGCCTCATTGGAAGAGGTTGTTGCTGCTTTAAAAATA
CTCTATGGCTATGATACTAAGATAAAGATGGAAAAGTTATATGAGGTTTCAAGAATTGTCTCAAGATTGATGAAACTTCC
TGTTCCACCAAATAAAGCAATTGTTGGGGACAATGCATTTGCTCATGAAGCAGGAATACATGTTGATGGATTAATAAAAA
ATACTGAAACCTATGAGCCAATAAAACCAGAAATGGTTGGGAATAGAAGAAGAATTATTTTGGGTAAGCATTCTGGTAGA
AAAGCTTTAAAATACAAACTTGATTTGATGGGCATAAACGTTAGTGATGAGCAATTAAATAAAATATATGAAAGAGTTAA
AGAATTTGGGGATTTGGGTAAATACATTTCAGACGCTGATTTGTTGGCTATAGTTAGAGAAGTTACTGGAAAATTGGTAG
AAGAGAAAATCAAATTAGATGAATTAACTGTTGTATCTGGAAATAAAATAACACCAATTGCATCTGTTAAACTCCATTAT
AAAGGAGAAGATATAACTTTAATAGAAACTGCTTATGGTGTTGGACCGGTAGATGCAGCAATAAATGCTGTGAGAAAGGC
AATAAGTGGAGTTGCAGATATTAAGTTGGTAGAGTATAGAGTTGAAGCAATTGGTGGAGGAACTGATGCGTTAATAGAGG
TTGTTGTTAAATTAAGAAAAGGAACTGAAATTGTTGAAGTTAGAAAATCAGACGCTGATATAATAAGGGCTTCTGTAGAT
GCTGTAATGGAAGGAATCAATATGTTATTGAATTAA

Upstream 100 bases:

>100_bases
AAATATACAATATCAAATATCTTTGAGATGATTCAATTTTATCATCAGATTCATTATGAGTATTTAAATTTCATTAAATT
ATTTTTTATGAGGGATTGTT

Downstream 100 bases:

>100_bases
TATTTTTGCTTATTTTAAAATGTTTTATTAGGTATTGATAATTTTAATATCAAAAGGTTTATATATCGGCTGTTATTATT
TTCAAATTGAAAACCTTGGA

Product: (R)-citramalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MMVRIFDTTLRDGEQTPGVSLTPNDKLEIAKKLDELGVDVIEAGSAITSKGEREGIKLITKEGLNAEICSFVRALPVDID
AALECDVDSVHLVVPTSPIHMKYKLRKTEDEVLETALKAVEYAKEHGLIVELSAEDATRSDVNFLIKLFNEGEKVGADRV
CVCDTVGVLTPQKSQELFKKITENVNLPVSVHCHNDFGMATANTCSAVLGGAVQCHVTVNGIGERAGNASLEEVVAALKI
LYGYDTKIKMEKLYEVSRIVSRLMKLPVPPNKAIVGDNAFAHEAGIHVDGLIKNTETYEPIKPEMVGNRRRIILGKHSGR
KALKYKLDLMGINVSDEQLNKIYERVKEFGDLGKYISDADLLAIVREVTGKLVEEKIKLDELTVVSGNKITPIASVKLHY
KGEDITLIETAYGVGPVDAAINAVRKAISGVADIKLVEYRVEAIGGGTDALIEVVVKLRKGTEIVEVRKSDADIIRASVD
AVMEGINMLLN

Sequences:

>Translated_491_residues
MMVRIFDTTLRDGEQTPGVSLTPNDKLEIAKKLDELGVDVIEAGSAITSKGEREGIKLITKEGLNAEICSFVRALPVDID
AALECDVDSVHLVVPTSPIHMKYKLRKTEDEVLETALKAVEYAKEHGLIVELSAEDATRSDVNFLIKLFNEGEKVGADRV
CVCDTVGVLTPQKSQELFKKITENVNLPVSVHCHNDFGMATANTCSAVLGGAVQCHVTVNGIGERAGNASLEEVVAALKI
LYGYDTKIKMEKLYEVSRIVSRLMKLPVPPNKAIVGDNAFAHEAGIHVDGLIKNTETYEPIKPEMVGNRRRIILGKHSGR
KALKYKLDLMGINVSDEQLNKIYERVKEFGDLGKYISDADLLAIVREVTGKLVEEKIKLDELTVVSGNKITPIASVKLHY
KGEDITLIETAYGVGPVDAAINAVRKAISGVADIKLVEYRVEAIGGGTDALIEVVVKLRKGTEIVEVRKSDADIIRASVD
AVMEGINMLLN
>Mature_491_residues
MMVRIFDTTLRDGEQTPGVSLTPNDKLEIAKKLDELGVDVIEAGSAITSKGEREGIKLITKEGLNAEICSFVRALPVDID
AALECDVDSVHLVVPTSPIHMKYKLRKTEDEVLETALKAVEYAKEHGLIVELSAEDATRSDVNFLIKLFNEGEKVGADRV
CVCDTVGVLTPQKSQELFKKITENVNLPVSVHCHNDFGMATANTCSAVLGGAVQCHVTVNGIGERAGNASLEEVVAALKI
LYGYDTKIKMEKLYEVSRIVSRLMKLPVPPNKAIVGDNAFAHEAGIHVDGLIKNTETYEPIKPEMVGNRRRIILGKHSGR
KALKYKLDLMGINVSDEQLNKIYERVKEFGDLGKYISDADLLAIVREVTGKLVEEKIKLDELTVVSGNKITPIASVKLHY
KGEDITLIETAYGVGPVDAAINAVRKAISGVADIKLVEYRVEAIGGGTDALIEVVVKLRKGTEIVEVRKSDADIIRASVD
AVMEGINMLLN

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family

Homologues:

Organism=Escherichia coli, GI1786261, Length=501, Percent_Identity=37.7245508982036, Blast_Score=298, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6320019, Length=353, Percent_Identity=33.7110481586402, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6320071, Length=373, Percent_Identity=32.7077747989276, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6324682, Length=549, Percent_Identity=21.1293260473588, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582381, Length=284, Percent_Identity=26.056338028169, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): CIMA_METJA (Q58787)

Other databases:

- EMBL:   L77117
- PIR:   G64473
- RefSeq:   NP_248395.1
- ProteinModelPortal:   Q58787
- SMR:   Q58787
- GeneID:   1452295
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1392
- NMPDR:   fig|243232.1.peg.1432
- TIGR:   MJ1392
- HOGENOM:   HBG701974
- OMA:   CFCDTVG
- ProtClustDB:   PRK09389
- BioCyc:   MetaCyc:MONOMER-11899
- BioCyc:   MJAN243232:MJ_1392-MONOMER
- BRENDA:   2.3.1.182
- HAMAP:   MF_01028
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.1.182

Molecular weight: Translated: 53494; Mature: 53494

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVRIFDTTLRDGEQTPGVSLTPNDKLEIAKKLDELGVDVIEAGSAITSKGEREGIKLIT
CEEEEEECHHCCCCCCCCCEECCCCHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCEEEEE
KEGLNAEICSFVRALPVDIDAALECDVDSVHLVVPTSPIHMKYKLRKTEDEVLETALKAV
CCCCCHHHHHHHHHCCCCCCCEEECCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHH
EYAKEHGLIVELSAEDATRSDVNFLIKLFNEGEKVGADRVCVCDTVGVLTPQKSQELFKK
HHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
ITENVNLPVSVHCHNDFGMATANTCSAVLGGAVQCHVTVNGIGERAGNASLEEVVAALKI
HHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
LYGYDTKIKMEKLYEVSRIVSRLMKLPVPPNKAIVGDNAFAHEAGIHVDGLIKNTETYEP
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHCCCEEEEEECCCCCCCC
IKPEMVGNRRRIILGKHSGRKALKYKLDLMGINVSDEQLNKIYERVKEFGDLGKYISDAD
CCHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHH
LLAIVREVTGKLVEEKIKLDELTVVSGNKITPIASVKLHYKGEDITLIETAYGVGPVDAA
HHHHHHHHHHHHHHHHHCCCEEEEECCCEECEEEEEEEEECCCCEEEEEECCCCCHHHHH
INAVRKAISGVADIKLVEYRVEAIGGGTDALIEVVVKLRKGTEIVEVRKSDADIIRASVD
HHHHHHHHCCHHHEEEEEEEHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
AVMEGINMLLN
HHHHHHHHHCC
>Mature Secondary Structure
MMVRIFDTTLRDGEQTPGVSLTPNDKLEIAKKLDELGVDVIEAGSAITSKGEREGIKLIT
CEEEEEECHHCCCCCCCCCEECCCCHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCEEEEE
KEGLNAEICSFVRALPVDIDAALECDVDSVHLVVPTSPIHMKYKLRKTEDEVLETALKAV
CCCCCHHHHHHHHHCCCCCCCEEECCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHH
EYAKEHGLIVELSAEDATRSDVNFLIKLFNEGEKVGADRVCVCDTVGVLTPQKSQELFKK
HHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
ITENVNLPVSVHCHNDFGMATANTCSAVLGGAVQCHVTVNGIGERAGNASLEEVVAALKI
HHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
LYGYDTKIKMEKLYEVSRIVSRLMKLPVPPNKAIVGDNAFAHEAGIHVDGLIKNTETYEP
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHCCCEEEEEECCCCCCCC
IKPEMVGNRRRIILGKHSGRKALKYKLDLMGINVSDEQLNKIYERVKEFGDLGKYISDAD
CCHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHH
LLAIVREVTGKLVEEKIKLDELTVVSGNKITPIASVKLHYKGEDITLIETAYGVGPVDAA
HHHHHHHHHHHHHHHHHCCCEEEEECCCEECEEEEEEEEECCCCEEEEEECCCCCHHHHH
INAVRKAISGVADIKLVEYRVEAIGGGTDALIEVVVKLRKGTEIVEVRKSDADIIRASVD
HHHHHHHHCCHHHEEEEEEEHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
AVMEGINMLLN
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 9864346