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The map label for this gene is gltX

Identifier: 15669567

GI number: 15669567

Start: 1325028

End: 1326689

Strand: Reverse

Name: gltX

Synonym: MJ1377

Alternate gene names: 15669567

Gene position: 1326689-1325028 (Counterclockwise)

Preceding gene: 15669572

Following gene: 15669565

Centisome position: 79.68

GC content: 33.39

Gene sequence:

>1662_bases
GTGGAAGAGAAGATATTGCCAATTGCATTAAGAAATGCCATAAAATACAATGGAAAAGCTAATCCAAAGGCAGTTTTAGG
GATATTTTTGTCAGAAAATCCAGAATATAGGAGTAAAGCAAAGGAGGTAATGCCAATTGTTGAGAAAGTTGTTGAAGAAG
TTAATAAACTATCATTGGATGAAATTAAGAAAAAGTTGGAAGAATTAGGAGAAGATGTTAAAAAGAAAGAAAAAAAGGAG
AAAGGTTTAGAATTACCAAACGTTAAAGATAAGGTAGTTATGAGATTCGCTCCTAATCCATCAGGGCCTTTACATATAGG
GCATGCAAGAGCAGCAGTTTTAAATGACTACTTTGTTAAAAAATATGGTGGAAAGTTAATTTTAAGATTAGAGGATACAG
ACCCAAAGAGAGTTCTGCCAGAAGCTTATGACATGATTAAAGAAGATTTGGATTGGCTGGGGGTTAAAGTTGATGAAGTG
GTTATACAATCAGATAGAATAGAGCTTTATTATGAATATGGTAGAAAATTGATTGAAATGGGACATGCTTATGTTTGTGA
CTGCAATCCAGAAGAATTTAGGGAATTGAGAAATAAAGGAGTTCCATGTAAGTGTAGAGATAGAGCCATTGAGGATAACT
TAGAGCTTTGGGAAAAGATGCTGAATGGAGAACTTGAAAATGTAGCTGTTAGATTAAAAACAGACATAAAACACAAAAAC
CCATCAATTAGGGACTTTCCAATATTCAGAGTTGAAAAAACTCCACATCCAAGAACTGGAGATAAATACTGTGTATATCC
TTTAATGAACTTCTCTGTTCCAGTTGATGATCATCTTTTAGGAATGACTCATGTTTTGAGAGGAAAAGACCACATTGTAA
ATACTGAGAAGCAAGCTTATATTTACAAATACTTTGGTTGGGAAATGCCAGAATTCATCCACTATGGGATTTTGAAGATA
GAGGACATTGTTTTAAGCACTTCATCAATGTATAAAGGAATTAAAGAAGGTCTCTATAGTGGATGGGATGACGTTAGATT
AGGAACTTTAAGAGCTTTAAGAAGAAGAGGGATTAAACCAGAGGCAATATATGAGATAATGAAAAGAATTGGAATTAAAC
AGGCAGATGTTAAGTTTTCTTGGGAGAATTTATATGCAATAAATAAGGAGCTTATTGATAAAGATGCAAGGAGATTCTTC
TTTGTCTGGAATCCAAAGAAACTTATTATCGAAGGGGCAGAGAAAAAGGTCTTAAAACTTAGAATGCATCCAGATAGACC
AGAATTTGGAGAGAGGGAGTTAATATTTGATGGAGAGGTTTATGTTGTTGGAGATGAGTTGGAAGAGAATAAGATGTATA
GATTGATGGAGTTATTTAACATAGTTGTTGAAAAAGTTGATGATATAGCATTAGCTAAATATCACTCAGATGACTTTAAA
ATAGCAAGGAAGAACAAAGCTAAGATTATACACTGGATTCCTGTAAAGGATAGTGTAAAGGTTAAAGTTTTAATGCCTGA
TGGAGAGATAAAGGAAGGCTTTGCTGAAAAAGATTTTGCTAAAGTAGAGGTTGATGATATTATCCAATTTGAGAGGTTTG
GATTTGTTAGAATAGATAAAAAAGATAATGATGGATTCGTATGTTGCTATGCACATAGATAA

Upstream 100 bases:

>100_bases
TAAAATTGCATAACTATATTTATGTTACTTAACTTTAAGTATCCTTTTCCTAATAATCAGTTAAGGTTTTTAAAGTTAAT
GGTAGGTAAATGGTGATAAT

Downstream 100 bases:

>100_bases
AAATAATTTTTTTATTTTTTAGATTTTAATTTCCTAATCTCTTTAATTTTTTTAGCTAAAAGTTCATTATCTTCATTTTC
AACTTTTTTAACCCTTTTTA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MEEKILPIALRNAIKYNGKANPKAVLGIFLSENPEYRSKAKEVMPIVEKVVEEVNKLSLDEIKKKLEELGEDVKKKEKKE
KGLELPNVKDKVVMRFAPNPSGPLHIGHARAAVLNDYFVKKYGGKLILRLEDTDPKRVLPEAYDMIKEDLDWLGVKVDEV
VIQSDRIELYYEYGRKLIEMGHAYVCDCNPEEFRELRNKGVPCKCRDRAIEDNLELWEKMLNGELENVAVRLKTDIKHKN
PSIRDFPIFRVEKTPHPRTGDKYCVYPLMNFSVPVDDHLLGMTHVLRGKDHIVNTEKQAYIYKYFGWEMPEFIHYGILKI
EDIVLSTSSMYKGIKEGLYSGWDDVRLGTLRALRRRGIKPEAIYEIMKRIGIKQADVKFSWENLYAINKELIDKDARRFF
FVWNPKKLIIEGAEKKVLKLRMHPDRPEFGERELIFDGEVYVVGDELEENKMYRLMELFNIVVEKVDDIALAKYHSDDFK
IARKNKAKIIHWIPVKDSVKVKVLMPDGEIKEGFAEKDFAKVEVDDIIQFERFGFVRIDKKDNDGFVCCYAHR

Sequences:

>Translated_553_residues
MEEKILPIALRNAIKYNGKANPKAVLGIFLSENPEYRSKAKEVMPIVEKVVEEVNKLSLDEIKKKLEELGEDVKKKEKKE
KGLELPNVKDKVVMRFAPNPSGPLHIGHARAAVLNDYFVKKYGGKLILRLEDTDPKRVLPEAYDMIKEDLDWLGVKVDEV
VIQSDRIELYYEYGRKLIEMGHAYVCDCNPEEFRELRNKGVPCKCRDRAIEDNLELWEKMLNGELENVAVRLKTDIKHKN
PSIRDFPIFRVEKTPHPRTGDKYCVYPLMNFSVPVDDHLLGMTHVLRGKDHIVNTEKQAYIYKYFGWEMPEFIHYGILKI
EDIVLSTSSMYKGIKEGLYSGWDDVRLGTLRALRRRGIKPEAIYEIMKRIGIKQADVKFSWENLYAINKELIDKDARRFF
FVWNPKKLIIEGAEKKVLKLRMHPDRPEFGERELIFDGEVYVVGDELEENKMYRLMELFNIVVEKVDDIALAKYHSDDFK
IARKNKAKIIHWIPVKDSVKVKVLMPDGEIKEGFAEKDFAKVEVDDIIQFERFGFVRIDKKDNDGFVCCYAHR
>Mature_553_residues
MEEKILPIALRNAIKYNGKANPKAVLGIFLSENPEYRSKAKEVMPIVEKVVEEVNKLSLDEIKKKLEELGEDVKKKEKKE
KGLELPNVKDKVVMRFAPNPSGPLHIGHARAAVLNDYFVKKYGGKLILRLEDTDPKRVLPEAYDMIKEDLDWLGVKVDEV
VIQSDRIELYYEYGRKLIEMGHAYVCDCNPEEFRELRNKGVPCKCRDRAIEDNLELWEKMLNGELENVAVRLKTDIKHKN
PSIRDFPIFRVEKTPHPRTGDKYCVYPLMNFSVPVDDHLLGMTHVLRGKDHIVNTEKQAYIYKYFGWEMPEFIHYGILKI
EDIVLSTSSMYKGIKEGLYSGWDDVRLGTLRALRRRGIKPEAIYEIMKRIGIKQADVKFSWENLYAINKELIDKDARRFF
FVWNPKKLIIEGAEKKVLKLRMHPDRPEFGERELIFDGEVYVVGDELEENKMYRLMELFNIVVEKVDDIALAKYHSDDFK
IARKNKAKIIHWIPVKDSVKVKVLMPDGEIKEGFAEKDFAKVEVDDIIQFERFGFVRIDKKDNDGFVCCYAHR

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=506, Percent_Identity=31.4229249011858, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI4826960, Length=320, Percent_Identity=31.875, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI134288884, Length=251, Percent_Identity=34.2629482071713, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1786895, Length=504, Percent_Identity=30.5555555555556, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1788743, Length=301, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI226510925, Length=297, Percent_Identity=29.2929292929293, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17511007, Length=359, Percent_Identity=33.7047353760446, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17543332, Length=502, Percent_Identity=28.0876494023904, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI71993394, Length=326, Percent_Identity=30.6748466257669, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321192, Length=527, Percent_Identity=33.3965844402277, Blast_Score=246, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6324742, Length=419, Percent_Identity=31.0262529832936, Blast_Score=210, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6324540, Length=296, Percent_Identity=32.0945945945946, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24649466, Length=537, Percent_Identity=29.7951582867784, Blast_Score=234, Evalue=9e-62,
Organism=Drosophila melanogaster, GI17864488, Length=500, Percent_Identity=29, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24665321, Length=256, Percent_Identity=34.375, Blast_Score=135, Evalue=8e-32,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_METJA (Q58772)

Other databases:

- EMBL:   L77117
- PIR:   H64471
- RefSeq:   NP_248380.1
- ProteinModelPortal:   Q58772
- SMR:   Q58772
- GeneID:   1452280
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1377
- NMPDR:   fig|243232.1.peg.1417
- TIGR:   MJ1377
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- ProtClustDB:   PRK04156
- BioCyc:   MJAN243232:MJ_1377-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR001412
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 64578; Mature: 64578

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKILPIALRNAIKYNGKANPKAVLGIFLSENPEYRSKAKEVMPIVEKVVEEVNKLSLD
CCCCEEHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
EIKKKLEELGEDVKKKEKKEKGLELPNVKDKVVMRFAPNPSGPLHIGHARAAVLNDYFVK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEECCHHHHHHHHHHHH
KYGGKLILRLEDTDPKRVLPEAYDMIKEDLDWLGVKVDEVVIQSDRIELYYEYGRKLIEM
HCCCEEEEEEECCCCHHCCHHHHHHHHHHHHHHCCCHHHEEEECCCEEHHHHHHHHHHHC
GHAYVCDCNPEEFRELRNKGVPCKCRDRAIEDNLELWEKMLNGELENVAVRLKTDIKHKN
CCEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCC
PSIRDFPIFRVEKTPHPRTGDKYCVYPLMNFSVPVDDHLLGMTHVLRGKDHIVNTEKQAY
CCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEE
IYKYFGWEMPEFIHYGILKIEDIVLSTSSMYKGIKEGLYSGWDDVRLGTLRALRRRGIKP
EEEECCCCCHHHHHHHHHEEEHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
EAIYEIMKRIGIKQADVKFSWENLYAINKELIDKDARRFFFVWNPKKLIIEGAEKKVLKL
HHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEE
RMHPDRPEFGERELIFDGEVYVVGDELEENKMYRLMELFNIVVEKVDDIALAKYHSDDFK
EECCCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCE
IARKNKAKIIHWIPVKDSVKVKVLMPDGEIKEGFAEKDFAKVEVDDIIQFERFGFVRIDK
EEECCCCEEEEEEECCCCEEEEEECCCCCHHCCCCCCCCCEEEHHHHHHHHHCCEEEEEC
KDNDGFVCCYAHR
CCCCCEEEEEECC
>Mature Secondary Structure
MEEKILPIALRNAIKYNGKANPKAVLGIFLSENPEYRSKAKEVMPIVEKVVEEVNKLSLD
CCCCEEHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
EIKKKLEELGEDVKKKEKKEKGLELPNVKDKVVMRFAPNPSGPLHIGHARAAVLNDYFVK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEECCHHHHHHHHHHHH
KYGGKLILRLEDTDPKRVLPEAYDMIKEDLDWLGVKVDEVVIQSDRIELYYEYGRKLIEM
HCCCEEEEEEECCCCHHCCHHHHHHHHHHHHHHCCCHHHEEEECCCEEHHHHHHHHHHHC
GHAYVCDCNPEEFRELRNKGVPCKCRDRAIEDNLELWEKMLNGELENVAVRLKTDIKHKN
CCEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCC
PSIRDFPIFRVEKTPHPRTGDKYCVYPLMNFSVPVDDHLLGMTHVLRGKDHIVNTEKQAY
CCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEE
IYKYFGWEMPEFIHYGILKIEDIVLSTSSMYKGIKEGLYSGWDDVRLGTLRALRRRGIKP
EEEECCCCCHHHHHHHHHEEEHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
EAIYEIMKRIGIKQADVKFSWENLYAINKELIDKDARRFFFVWNPKKLIIEGAEKKVLKL
HHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEE
RMHPDRPEFGERELIFDGEVYVVGDELEENKMYRLMELFNIVVEKVDDIALAKYHSDDFK
EECCCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCE
IARKNKAKIIHWIPVKDSVKVKVLMPDGEIKEGFAEKDFAKVEVDDIIQFERFGFVRIDK
EEECCCCEEEEEEECCCCEEEEEECCCCCHHCCCCCCCCCEEEHHHHHHHHHCCEEEEEC
KDNDGFVCCYAHR
CCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087