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The map label for this gene is wtpB

Identifier: 15669558

GI number: 15669558

Start: 1317687

End: 1318436

Strand: Reverse

Name: wtpB

Synonym: MJ1368

Alternate gene names: 15669558

Gene position: 1318436-1317687 (Counterclockwise)

Preceding gene: 15669565

Following gene: 15669557

Centisome position: 79.19

GC content: 32.4

Gene sequence:

>750_bases
ATGGAGAAGTTCGATATTGCGATGACAGTGTTTTTGGTAATGATATTCTTATTCATATTTTTACCAATTATTTATATGCT
ATCAAATCCCGGAGATTTAAACCAATTGTTGGATAAAGAGGTTATAGAGGCGTTTAAAACTACTCTATTAGCTGGAGCTG
TTGCTACTCTAATAGCTCTAATTTTTGGAATACCAACTGGCTATATTTTGGCAAGGTATGATTTTAAATTTAAAAGCTTT
GTTGAGGCTGTTTTAGATTTACCGATGGCAATTCCTCACAGCGTTATAGGTATCATAATCCTATCCTTCATTTATGGTAT
TGATATTATAAATTTTATTGGTAGATATGTAGTTGATAACTTTTGGGGGATTGTTACTGTCTATCTATTTGTTGGCATAC
CTTTTATGGTTAATAGTATAAGAGATGGCTTTTTAAGTGTTGATGAAGAGATTGAGTATGTCTCAAGAACCTTGGGGGCT
TCAAAGATAAGGACGTTTTTTGAAATATCTCTCCCATTGATAAAAAATAATATCATCTCTGGGATTATTTTGAGTTTTGC
AAGAGGAATTAGTGAGGTTGGAGCAATATTGATAATAGCATATTATCCAAAAACAGTTCCTATCTTAATATATGAAAGAT
TTATGAGCTTTGGATTAGATGCTTCAAAACCAATATCTGTTGGAATGATTTTGATTAGCATAGCGTTGTTTGCATTACTA
AGGATGTTTGGGAGGATGAGAGGGAGATAA

Upstream 100 bases:

>100_bases
TATTATACTATCAAATGTCATCAATTTTGTTTAACACAAATTTTATATAATTAGGTAATTTAATTACCTTAAAAATGATT
AAGATTGATTAGGGATAGGC

Downstream 100 bases:

>100_bases
TGCTTAAAGTAAATAATCTATCAAAGATTTGGAAAGATTTTAAATTAAAGAATGTCTCTTTTGAAATAGATAGGGAGTAT
TGTGTAATTCTCGGTCCAAG

Product: sulfate transport system permease CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MEKFDIAMTVFLVMIFLFIFLPIIYMLSNPGDLNQLLDKEVIEAFKTTLLAGAVATLIALIFGIPTGYILARYDFKFKSF
VEAVLDLPMAIPHSVIGIIILSFIYGIDIINFIGRYVVDNFWGIVTVYLFVGIPFMVNSIRDGFLSVDEEIEYVSRTLGA
SKIRTFFEISLPLIKNNIISGIILSFARGISEVGAILIIAYYPKTVPILIYERFMSFGLDASKPISVGMILISIALFALL
RMFGRMRGR

Sequences:

>Translated_249_residues
MEKFDIAMTVFLVMIFLFIFLPIIYMLSNPGDLNQLLDKEVIEAFKTTLLAGAVATLIALIFGIPTGYILARYDFKFKSF
VEAVLDLPMAIPHSVIGIIILSFIYGIDIINFIGRYVVDNFWGIVTVYLFVGIPFMVNSIRDGFLSVDEEIEYVSRTLGA
SKIRTFFEISLPLIKNNIISGIILSFARGISEVGAILIIAYYPKTVPILIYERFMSFGLDASKPISVGMILISIALFALL
RMFGRMRGR
>Mature_249_residues
MEKFDIAMTVFLVMIFLFIFLPIIYMLSNPGDLNQLLDKEVIEAFKTTLLAGAVATLIALIFGIPTGYILARYDFKFKSF
VEAVLDLPMAIPHSVIGIIILSFIYGIDIINFIGRYVVDNFWGIVTVYLFVGIPFMVNSIRDGFLSVDEEIEYVSRTLGA
SKIRTFFEISLPLIKNNIISGIILSFARGISEVGAILIIAYYPKTVPILIYERFMSFGLDASKPISVGMILISIALFALL
RMFGRMRGR

Specific function: Part of the ABC transporter complex wtpABC involved in molybdate/tungstate import. Probably responsible for the translocation of the substrate across the membrane

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1788764, Length=200, Percent_Identity=28.5, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786980, Length=231, Percent_Identity=32.9004329004329, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87082099, Length=175, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WTPB_METJA (Q58763)

Other databases:

- EMBL:   L77117
- PIR:   G64470
- RefSeq:   NP_248371.1
- ProteinModelPortal:   Q58763
- SMR:   Q58763
- GeneID:   1452271
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1368
- NMPDR:   fig|243232.1.peg.1408
- TIGR:   MJ1368
- HOGENOM:   HBG685300
- OMA:   LIFASYP
- ProtClustDB:   CLSK876550
- BioCyc:   MJAN243232:MJ_1368-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 27942; Mature: 27942

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbe89470)-; HASH(0xea13138)-; HASH(0xe9adfc4)-; HASH(0xcbf481c)-; HASH(0xe566168)-; HASH(0xe9ad5b4)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFDIAMTVFLVMIFLFIFLPIIYMLSNPGDLNQLLDKEVIEAFKTTLLAGAVATLIAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFGIPTGYILARYDFKFKSFVEAVLDLPMAIPHSVIGIIILSFIYGIDIINFIGRYVVDN
HHHCCCCHHHHEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWGIVTVYLFVGIPFMVNSIRDGFLSVDEEIEYVSRTLGASKIRTFFEISLPLIKNNIIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIILSFARGISEVGAILIIAYYPKTVPILIYERFMSFGLDASKPISVGMILISIALFALL
HHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
RMFGRMRGR
HHHHHHCCH
>Mature Secondary Structure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HHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087