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The map label for this gene is hyfB [C]

Identifier: 15669499

GI number: 15669499

Start: 1254451

End: 1255896

Strand: Reverse

Name: hyfB [C]

Synonym: MJ1309

Alternate gene names: 15669499

Gene position: 1255896-1254451 (Counterclockwise)

Preceding gene: 15669500

Following gene: 15669498

Centisome position: 75.43

GC content: 35.2

Gene sequence:

>1446_bases
ATGAATTATCTGCCGATGATGATAGTGTTTCCATTAATCATGGCAATAATAATGAATTTATTGCATGGAAAAGAGAAAGC
AGTAAAATATATAACATTTATTACAGCTGCTATTTTGATTATTTTGCCATTTATCAGCCAGTATGGTTATTATTACTTTG
GTGGGCATGGAGTTGTTAATGGATGGGTATCTGGTATTGCCTATCTATATAACCCAGCAAAGCAGGCAATTATTGTAACT
CTGTCTTTAATTGCCTCTCTTGTTTTAATTACAGGAATGGGAGAGAAATTAAAGAATAATATGTTTGTTACCCTCTCATT
AATGGGATTTGCAAGTATTGCAGCTATAGTTTTGGCTGATGATATATTCAACTTGTATGTGTTCTTTGAGATAGTTTCAA
TTGTCCAAGCTGGATTAGTATTTTTATCTGGAACTGAAGAGGCATATAAAGCAGGATTAAGATATATGATAATGGGGAAT
GTTGCGGCAGCCTTAATGCTATTAGGAATAGCGTTCTTATTAGCTTCAACTGGAACTCTAAACATCACAGACATGAAACA
CTATCTGTTAGTTGATAATCCAATGATTTATGGTGGCTTGTTGTTGCTAATTGTTGGTTTAGCTTATGGGGCTGGATTGC
CGCCATTCCACAACGTTAAAGCTGATTTATACGCAAGGTCTAAGGGATTTATCTCTGCAATGCTCCAAACATACTCAAAA
TTTGTGTTAGTTGGCTTGATGATAATTATTCTAAAATTATTTAATGGATTAGATTATTTTGCAAGTGCTCATGCTGTTTT
AATTGCATTGGGAGTTTTGGCAATGGTATTTGGGGTTGTAATGGCGTTATTGCAAAGTGATTATAAAAAGCTTTTGGCAT
ATCACGCTATAAGTCAAGGTGGCTATGTGGCTACTGGCTTAGCTTTAGGAACACCATTAGGAATTGTTGCTGGTATCTTC
CACGCTATAAATCACGTTATTTATAAATCTGCCTTGTTTTTGGGGGCGTATATTGTAAGCTGTAAGAGAGGAAGTAATTT
GCATAAGTTGGGAGGTTTATTGCCTCTAATGCCCTCTGTGGCATTTATGGTTTTATGTGCAAAGCTTGCGATTAGTGGAA
TTCCACCATTTAACGGATTTCAGAGTAAGTGGATGCTTGCCCAAGCAGCTATGCAAGTGAATATGCCAGAAATAGCTATA
ATAATGATTATTGTTAGTATAGGGACGTTTGTCTCAATGATGAAGGCATTCTATTTAATTTACTTAAAACCAGTTGATGA
AGAAACTCTGAAAGAGTATCAAAACAAGGAAGTTCCTAAACTTGCTGTCTTTAGCTTGTTTGTATTAACTGCTCTATGCA
TAATAATTGGTCTCTATCCAGACATTGTAACAAACTATCTCTGGGACTATGCAAAGGAGTTAGGGGTTAATTATTATTTA
AAATAG

Upstream 100 bases:

>100_bases
TCTATAATTTTGTATAAAAAATATAAAACACTCAGAAGCTCTGTAATACTAAAAGAGGATTAATTCTATTTAACTTTCTC
TAAAAGATTGGAGGGAGAAT

Downstream 100 bases:

>100_bases
ACAAAATAACTTAATTTTTGGTGGATTTTATGGATTATAATGACTTTCAAAAAAAGTTGGATAAAGAAGAGCATGGGGAT
GGAATCACAGTTGGAGCAGT

Product: hydrogenase subunit F

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MNYLPMMIVFPLIMAIIMNLLHGKEKAVKYITFITAAILIILPFISQYGYYYFGGHGVVNGWVSGIAYLYNPAKQAIIVT
LSLIASLVLITGMGEKLKNNMFVTLSLMGFASIAAIVLADDIFNLYVFFEIVSIVQAGLVFLSGTEEAYKAGLRYMIMGN
VAAALMLLGIAFLLASTGTLNITDMKHYLLVDNPMIYGGLLLLIVGLAYGAGLPPFHNVKADLYARSKGFISAMLQTYSK
FVLVGLMIIILKLFNGLDYFASAHAVLIALGVLAMVFGVVMALLQSDYKKLLAYHAISQGGYVATGLALGTPLGIVAGIF
HAINHVIYKSALFLGAYIVSCKRGSNLHKLGGLLPLMPSVAFMVLCAKLAISGIPPFNGFQSKWMLAQAAMQVNMPEIAI
IMIIVSIGTFVSMMKAFYLIYLKPVDEETLKEYQNKEVPKLAVFSLFVLTALCIIIGLYPDIVTNYLWDYAKELGVNYYL
K

Sequences:

>Translated_481_residues
MNYLPMMIVFPLIMAIIMNLLHGKEKAVKYITFITAAILIILPFISQYGYYYFGGHGVVNGWVSGIAYLYNPAKQAIIVT
LSLIASLVLITGMGEKLKNNMFVTLSLMGFASIAAIVLADDIFNLYVFFEIVSIVQAGLVFLSGTEEAYKAGLRYMIMGN
VAAALMLLGIAFLLASTGTLNITDMKHYLLVDNPMIYGGLLLLIVGLAYGAGLPPFHNVKADLYARSKGFISAMLQTYSK
FVLVGLMIIILKLFNGLDYFASAHAVLIALGVLAMVFGVVMALLQSDYKKLLAYHAISQGGYVATGLALGTPLGIVAGIF
HAINHVIYKSALFLGAYIVSCKRGSNLHKLGGLLPLMPSVAFMVLCAKLAISGIPPFNGFQSKWMLAQAAMQVNMPEIAI
IMIIVSIGTFVSMMKAFYLIYLKPVDEETLKEYQNKEVPKLAVFSLFVLTALCIIIGLYPDIVTNYLWDYAKELGVNYYL
K
>Mature_481_residues
MNYLPMMIVFPLIMAIIMNLLHGKEKAVKYITFITAAILIILPFISQYGYYYFGGHGVVNGWVSGIAYLYNPAKQAIIVT
LSLIASLVLITGMGEKLKNNMFVTLSLMGFASIAAIVLADDIFNLYVFFEIVSIVQAGLVFLSGTEEAYKAGLRYMIMGN
VAAALMLLGIAFLLASTGTLNITDMKHYLLVDNPMIYGGLLLLIVGLAYGAGLPPFHNVKADLYARSKGFISAMLQTYSK
FVLVGLMIIILKLFNGLDYFASAHAVLIALGVLAMVFGVVMALLQSDYKKLLAYHAISQGGYVATGLALGTPLGIVAGIF
HAINHVIYKSALFLGAYIVSCKRGSNLHKLGGLLPLMPSVAFMVLCAKLAISGIPPFNGFQSKWMLAQAAMQVNMPEIAI
IMIIVSIGTFVSMMKAFYLIYLKPVDEETLKEYQNKEVPKLAVFSLFVLTALCIIIGLYPDIVTNYLWDYAKELGVNYYL
K

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788827, Length=385, Percent_Identity=29.3506493506493, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788831, Length=293, Percent_Identity=28.3276450511945, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788829, Length=332, Percent_Identity=29.2168674698795, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI2367154, Length=370, Percent_Identity=27.5675675675676, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI145693160, Length=293, Percent_Identity=27.6450511945392, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788614, Length=358, Percent_Identity=27.0949720670391, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788613, Length=411, Percent_Identity=23.3576642335766, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1309_METJA (Q58705)

Other databases:

- EMBL:   L77117
- PIR:   D64463
- RefSeq:   NP_248309.1
- ProteinModelPortal:   Q58705
- GeneID:   1452211
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1309
- NMPDR:   fig|243232.1.peg.1346
- TIGR:   MJ1309
- HOGENOM:   HBG541027
- OMA:   CAKLAIS
- ProtClustDB:   PRK06277
- BioCyc:   MJAN243232:MJ_1309-MONOMER
- InterPro:   IPR001750

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52544; Mature: 52544

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe9aadd0)-; HASH(0xe9479f8)-; HASH(0xe72e048)-; HASH(0xe70ae44)-; HASH(0xe947af4)-; HASH(0xe70afb8)-; HASH(0xe72def8)-; HASH(0xe72df40)-; HASH(0xe70af04)-; HASH(0xe43c7e8)-; HASH(0xe9455cc)-; HASH(0xe9455a8)-; HASH(0xe925bfc)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLPMMIVFPLIMAIIMNLLHGKEKAVKYITFITAAILIILPFISQYGYYYFGGHGVVN
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHH
GWVSGIAYLYNPAKQAIIVTLSLIASLVLITGMGEKLKNNMFVTLSLMGFASIAAIVLAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEHHHHHHHHHHHHHHH
DIFNLYVFFEIVSIVQAGLVFLSGTEEAYKAGLRYMIMGNVAAALMLLGIAFLLASTGTL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
NITDMKHYLLVDNPMIYGGLLLLIVGLAYGAGLPPFHNVKADLYARSKGFISAMLQTYSK
EEECCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FVLVGLMIIILKLFNGLDYFASAHAVLIALGVLAMVFGVVMALLQSDYKKLLAYHAISQG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYVATGLALGTPLGIVAGIFHAINHVIYKSALFLGAYIVSCKRGSNLHKLGGLLPLMPSV
CCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHH
AFMVLCAKLAISGIPPFNGFQSKWMLAQAAMQVNMPEIAIIMIIVSIGTFVSMMKAFYLI
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YLKPVDEETLKEYQNKEVPKLAVFSLFVLTALCIIIGLYPDIVTNYLWDYAKELGVNYYL
HCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
K
H
>Mature Secondary Structure
MNYLPMMIVFPLIMAIIMNLLHGKEKAVKYITFITAAILIILPFISQYGYYYFGGHGVVN
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHH
GWVSGIAYLYNPAKQAIIVTLSLIASLVLITGMGEKLKNNMFVTLSLMGFASIAAIVLAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEHHHHHHHHHHHHHHH
DIFNLYVFFEIVSIVQAGLVFLSGTEEAYKAGLRYMIMGNVAAALMLLGIAFLLASTGTL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
NITDMKHYLLVDNPMIYGGLLLLIVGLAYGAGLPPFHNVKADLYARSKGFISAMLQTYSK
EEECCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FVLVGLMIIILKLFNGLDYFASAHAVLIALGVLAMVFGVVMALLQSDYKKLLAYHAISQG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYVATGLALGTPLGIVAGIFHAINHVIYKSALFLGAYIVSCKRGSNLHKLGGLLPLMPSV
CCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHH
AFMVLCAKLAISGIPPFNGFQSKWMLAQAAMQVNMPEIAIIMIIVSIGTFVSMMKAFYLI
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YLKPVDEETLKEYQNKEVPKLAVFSLFVLTALCIIIGLYPDIVTNYLWDYAKELGVNYYL
HCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
K
H

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087