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The map label for this gene is hsdM [C]

Identifier: 15669405

GI number: 15669405

Start: 1162253

End: 1163989

Strand: Reverse

Name: hsdM [C]

Synonym: MJ1220

Alternate gene names: 15669405

Gene position: 1163989-1162253 (Counterclockwise)

Preceding gene: 15669407

Following gene: 15669404

Centisome position: 69.91

GC content: 31.16

Gene sequence:

>1737_bases
TTGAAACTTAGAAATGTTGAACCAAGGTTTTTGAAAGCATATAATATATTAATGGATAAATTTGGGCTATTCCCATTTAC
CTATGATATGGCTGAAAAGGTTCTTAAAGATAACTATGAAAATGTGAATGAAGTATTATCCAAGTTGGCTGATGCTGGAT
TATTGGAAAAGACAGCAAAGAAGGAAGATAAAAGAAAGAAAATTTATAAAATAAAGCCATTAACTACTGAAAAAATTGAA
AAGGTAAGTAAAGATAAGTTAATTGGTTTGCTTAAGCAAGGGGCTGATTTGATAAGAACGCAGGTAGATTATAAAGTATT
ACTGTTATTTTTGTTTTTTAAGGCAATTAGTGATAAATATCTGTTAAAAGTTGAGGAGTTGAAGAAGGAGTTTGAAGATT
TGGATGAAGAAGATATATATGTATTGGCAAATGAGGAAATTTTAGAGCTTTATGATGTTGAGGGTAAAAAGTTGTATGTA
TGGCATGAAGTAGCAAATAATCCAGAAGATTTTATAAATGCATTAAATAAAATTGTTGAGATGAATAAGGAGAAATTGAG
TGGTTTAGATGAGTTGATAAAAAGAACTGGACTTCCTACATTATTTGAAAATGAAAATAGGCATATTGTTCAACATTTAA
TTAATTTATTTAGTAGAGCAGATTTTTCAGAAGCATCTTATGATATATTGGGAGATGCTTATGAATGGACTTTAAATTAT
TTTGCTCCAACAAAGGCAAAAGAGGGGGAGGTTTATACTCCTATTGAAGTTAGCAAACTAATTGCCCATTTGGTTGAACC
AAAAGACGATGAGGTAATTTTAGACCCTGCATGTGGTTCTGGTTCTATGTTGATAGAGCAGTATAGATTTGCAGGTAGTA
ATCCAAATATTGTGTTGGTTGGGCAAGAAAGGAATGATGTTACTGCCGTTTTAGCAAAGTTGAATTTTATACTGCATGGA
ATTAACTTAAAAGATGCTAAGGTGTTTATTGGAGATTCTTTACTAAATCCAAAGTTTGAGAGTTTTATTNAAGAAGTTAA
AGGTACTGGAAAAGCTGATAAGGTTGTAGCAAATCCACCATGGAATCAGGATGGTTACGATGAAAACACCCTAAAAGTGA
ATGAAAAATATAAAGATATTTATATGTATGGATTTCCAAATAAAAACTCCGCTGATTGGGCATGGGTTCAGTTGATAAAT
TATTATACTGAAAAAAAGGCGGGGATTGTTTTAGATTCAGGGGCTTTGTTTAGGGGAGGGAAAGAGAAGACAATAAGGAA
GAGATTTGTAGATGATGATTTAATTGAGGCAGTTGTTTTATTGCCTGAGAAGTTATTTTATAACTGTCCTGCACCAGGGA
TTATTTTAATTTTGAATAAAAATAAGCCAGAAGAGAGAAAAGGAAAGATTTTGTTTATAAATGCCTCTAATGAATATATT
AAACATCCAGAGGTTAAAAAATTAAACAAACTCTCTGATGAGAACATTGAGAAAATAGCAAAGGCATATAAAGAGTTTAA
GGATGTTGATGGCTTTTGTAAGGTTGTAGATATTGAGGAGATTAGAAAGAATGATTATAATCTAAATGTTTCTTTGTATA
TCTCTCCAATTGAAGAAGATGAGGATGTTGATTTAGGAGAGGTTTATGAAGAGCTTAATAAATTGCATAATGAGTATTTG
GAGAAGTTTGAGGTTGTTAAAGGTTATTTAGAGGAGATTAATGGGTTGATTAAATAG

Upstream 100 bases:

>100_bases
TCATGTATCTCATTCTCTCACCAAACCACAACCTTTATATACACCCTACATCCAATTACATAGTGAAAACATTAAAATAA
CTATCTAAGTTGGTGATACC

Downstream 100 bases:

>100_bases
ATATTTTTTTGAGGGATTTTAAGAGCTGGAAGTTAATTTAATTTGTTTGATTGATTAGAAATAAGTAAGGTGGTTAATAT
GGCTCCAAATACAAATTTTG

Product: type I restriction-modification enzyme 1 subunit M

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKLRNVEPRFLKAYNILMDKFGLFPFTYDMAEKVLKDNYENVNEVLSKLADAGLLEKTAKKEDKRKKIYKIKPLTTEKIE
KVSKDKLIGLLKQGADLIRTQVDYKVLLLFLFFKAISDKYLLKVEELKKEFEDLDEEDIYVLANEEILELYDVEGKKLYV
WHEVANNPEDFINALNKIVEMNKEKLSGLDELIKRTGLPTLFENENRHIVQHLINLFSRADFSEASYDILGDAYEWTLNY
FAPTKAKEGEVYTPIEVSKLIAHLVEPKDDEVILDPACGSGSMLIEQYRFAGSNPNIVLVGQERNDVTAVLAKLNFILHG
INLKDAKVFIGDSLLNPKFESFIXEVKGTGKADKVVANPPWNQDGYDENTLKVNEKYKDIYMYGFPNKNSADWAWVQLIN
YYTEKKAGIVLDSGALFRGGKEKTIRKRFVDDDLIEAVVLLPEKLFYNCPAPGIILILNKNKPEERKGKILFINASNEYI
KHPEVKKLNKLSDENIEKIAKAYKEFKDVDGFCKVVDIEEIRKNDYNLNVSLYISPIEEDEDVDLGEVYEELNKLHNEYL
EKFEVVKGYLEEINGLIK

Sequences:

>Translated_578_residues
MKLRNVEPRFLKAYNILMDKFGLFPFTYDMAEKVLKDNYENVNEVLSKLADAGLLEKTAKKEDKRKKIYKIKPLTTEKIE
KVSKDKLIGLLKQGADLIRTQVDYKVLLLFLFFKAISDKYLLKVEELKKEFEDLDEEDIYVLANEEILELYDVEGKKLYV
WHEVANNPEDFINALNKIVEMNKEKLSGLDELIKRTGLPTLFENENRHIVQHLINLFSRADFSEASYDILGDAYEWTLNY
FAPTKAKEGEVYTPIEVSKLIAHLVEPKDDEVILDPACGSGSMLIEQYRFAGSNPNIVLVGQERNDVTAVLAKLNFILHG
INLKDAKVFIGDSLLNPKFESFIXEVKGTGKADKVVANPPWNQDGYDENTLKVNEKYKDIYMYGFPNKNSADWAWVQLIN
YYTEKKAGIVLDSGALFRGGKEKTIRKRFVDDDLIEAVVLLPEKLFYNCPAPGIILILNKNKPEERKGKILFINASNEYI
KHPEVKKLNKLSDENIEKIAKAYKEFKDVDGFCKVVDIEEIRKNDYNLNVSLYISPIEEDEDVDLGEVYEELNKLHNEYL
EKFEVVKGYLEEINGLIK
>Mature_578_residues
MKLRNVEPRFLKAYNILMDKFGLFPFTYDMAEKVLKDNYENVNEVLSKLADAGLLEKTAKKEDKRKKIYKIKPLTTEKIE
KVSKDKLIGLLKQGADLIRTQVDYKVLLLFLFFKAISDKYLLKVEELKKEFEDLDEEDIYVLANEEILELYDVEGKKLYV
WHEVANNPEDFINALNKIVEMNKEKLSGLDELIKRTGLPTLFENENRHIVQHLINLFSRADFSEASYDILGDAYEWTLNY
FAPTKAKEGEVYTPIEVSKLIAHLVEPKDDEVILDPACGSGSMLIEQYRFAGSNPNIVLVGQERNDVTAVLAKLNFILHG
INLKDAKVFIGDSLLNPKFESFIXEVKGTGKADKVVANPPWNQDGYDENTLKVNEKYKDIYMYGFPNKNSADWAWVQLIN
YYTEKKAGIVLDSGALFRGGKEKTIRKRFVDDDLIEAVVLLPEKLFYNCPAPGIILILNKNKPEERKGKILFINASNEYI
KHPEVKKLNKLSDENIEKIAKAYKEFKDVDGFCKVVDIEEIRKNDYNLNVSLYISPIEEDEDVDLGEVYEELNKLHNEYL
EKFEVVKGYLEEINGLIK

Specific function: The M And S Subunits Together Form A Methyltransferase (Mtase) That Methylates Two Adenine Residues In Complementary Strands Of A Bipartite DNA Recognition Sequence. In The Presence Of The R Subunit The Complex Can Also Act As An Endonuclease, Binding To

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI1790808, Length=345, Percent_Identity=24.9275362318841, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1220_METJA (Q58617)

Other databases:

- EMBL:   L77117
- PIR:   C64452
- RefSeq:   NP_248215.1
- ProteinModelPortal:   Q58617
- REBASE:   3905
- PRIDE:   Q58617
- GeneID:   1452116
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1220
- NMPDR:   fig|243232.1.peg.1252
- TIGR:   MJ1220
- HOGENOM:   HBG515557
- OMA:   DTIRNPL
- ProtClustDB:   CLSK688100
- BioCyc:   MJAN243232:MJ_1220-MONOMER
- BRENDA:   2.1.1.72
- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00507

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase

EC number: =2.1.1.72

Molecular weight: Translated: 66584; Mature: 66584

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRNVEPRFLKAYNILMDKFGLFPFTYDMAEKVLKDNYENVNEVLSKLADAGLLEKTAK
CCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
KEDKRKKIYKIKPLTTEKIEKVSKDKLIGLLKQGADLIRTQVDYKVLLLFLFFKAISDKY
HHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHH
LLKVEELKKEFEDLDEEDIYVLANEEILELYDVEGKKLYVWHEVANNPEDFINALNKIVE
HHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCEEEEEEHHCCCHHHHHHHHHHHHH
MNKEKLSGLDELIKRTGLPTLFENENRHIVQHLINLFSRADFSEASYDILGDAYEWTLNY
CCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCHHHCCCHHEEEEEE
FAPTKAKEGEVYTPIEVSKLIAHLVEPKDDEVILDPACGSGSMLIEQYRFAGSNPNIVLV
ECCCCCCCCCEECCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEE
GQERNDVTAVLAKLNFILHGINLKDAKVFIGDSLLNPKFESFIXEVKGTGKADKVVANPP
ECCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCC
WNQDGYDENTLKVNEKYKDIYMYGFPNKNSADWAWVQLINYYTEKKAGIVLDSGALFRGG
CCCCCCCCCEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCC
KEKTIRKRFVDDDLIEAVVLLPEKLFYNCPAPGIILILNKNKPEERKGKILFINASNEYI
CHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCEEEEECCCCCC
KHPEVKKLNKLSDENIEKIAKAYKEFKDVDGFCKVVDIEEIRKNDYNLNVSLYISPIEED
CCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCEEEEEEEEEECCCC
EDVDLGEVYEELNKLHNEYLEKFEVVKGYLEEINGLIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLRNVEPRFLKAYNILMDKFGLFPFTYDMAEKVLKDNYENVNEVLSKLADAGLLEKTAK
CCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
KEDKRKKIYKIKPLTTEKIEKVSKDKLIGLLKQGADLIRTQVDYKVLLLFLFFKAISDKY
HHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHH
LLKVEELKKEFEDLDEEDIYVLANEEILELYDVEGKKLYVWHEVANNPEDFINALNKIVE
HHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCEEEEEEHHCCCHHHHHHHHHHHHH
MNKEKLSGLDELIKRTGLPTLFENENRHIVQHLINLFSRADFSEASYDILGDAYEWTLNY
CCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCHHHCCCHHEEEEEE
FAPTKAKEGEVYTPIEVSKLIAHLVEPKDDEVILDPACGSGSMLIEQYRFAGSNPNIVLV
ECCCCCCCCCEECCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEE
GQERNDVTAVLAKLNFILHGINLKDAKVFIGDSLLNPKFESFIXEVKGTGKADKVVANPP
ECCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCC
WNQDGYDENTLKVNEKYKDIYMYGFPNKNSADWAWVQLINYYTEKKAGIVLDSGALFRGG
CCCCCCCCCEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCC
KEKTIRKRFVDDDLIEAVVLLPEKLFYNCPAPGIILILNKNKPEERKGKILFINASNEYI
CHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCEEEEECCCCCC
KHPEVKKLNKLSDENIEKIAKAYKEFKDVDGFCKVVDIEEIRKNDYNLNVSLYISPIEED
CCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCEEEEEEEEEECCCC
EDVDLGEVYEELNKLHNEYLEKFEVVKGYLEEINGLIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087