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The map label for this gene is asnB [C]

Identifier: 15669303

GI number: 15669303

Start: 1056676

End: 1058310

Strand: Reverse

Name: asnB [C]

Synonym: MJ1116

Alternate gene names: 15669303

Gene position: 1058310-1056676 (Counterclockwise)

Preceding gene: 15669308

Following gene: 15669302

Centisome position: 63.56

GC content: 34.8

Gene sequence:

>1635_bases
GTGAGAGACATGTGCTCTATAAGTGGAATAATTGTTAAAGACAATCAAATATCTGCTAAATACTCCATAGATATGATGAA
GATTTTAAAGCACAGAGGGAGAGATAACTCTGGGCTGTTGTTGGATGATGAAGTTATATATTTTAACGATTTTGAGGATG
TTGAGGATTTAGAGGAGGAGATGATTGGAAACTTAAGCTTGGCTCATAATAGATTGGCAATTGTTGGGAGGTATGGAGTT
CAACCTATTCCAAATGAGGATGAAACTATATGGTTAGTTTGTAATGGAGAGATTTACAATTATATTGAGTTGAGGGAATA
TCTAAAACAAAATCATGAATTTAGGACAGACAGTGATAATGAGGTTATAATTCATCTATATGAAGAGGAGAAGTTGGAAG
AGTTGGATGGAGACTATGCCTTTGCCATATATGATAAATCTAAGAATGTTGTGAGGTTGGCAAGGGATATGTTTGGAGTT
AAGCCATTATTTTATGTAGATAGGGATAAATACTTTGCCTTTGCCTCTGAAAGAAAGGCGTTGTGGCATCTACTTATAAA
TATCGATGGCTGTGAGAGAGATTTAGATGAGCTAAATAGCAAAATCAAAACATTGAAGCCAAATTCACAGTTGATTTATT
ATTTAGATGATAATAGGTTTGAAATTATTGAAGGCTTTAAAAAGTTGGAGTTAAATTACATGAAAGAGAGGAGTTATGAG
GAGGCTAAGGAGTATTTAGATAGAGCATTGAAAAACTCTGTTTTAAAGAGGGTCAGGGGTTTGGACAAAGTTGGAATTAT
ATGCTCTGGAGGAGTTGATAGCTCATTGATTGCTAAATTAGCATCTCTATACTGTGAAGTTATATTGTATGCCGTTGGAA
CTGAAAATAGTGAAGATTTAATCTATGCTGAAAGATTGGCTAAAGATTTAAATTTAAAGCTAAGGAAGAAGATTATTTCA
GAAGAGGAGTATGAGGAGTATGTGTTTAAGGTAGCTAAGGCAATAGATGAAGTTGATTTAATGAAGATTGGAGTTGGAAT
CCCTATCTATGTAGCTTCAGAGATGGCAAATGAGGATGGATTAAAGGTTGTTCTATCTGGGCAAGGAGCTGATGAGTTAT
TTGGAGGCTATGCAAGGCATGAGAGAATTTATAGGGAGAGAGGGGAGGAGGAGCTGAAAAAAGAGCTATTGAAGGATGTT
TATAATTTATATAAGGTAAATTTAGAGAGAGATGACCACTGTACAATGGCTAATGGTGTTGAGTTGAGAGTTCCTTTCTT
AGATGAGGAGGTTGTTGAAATTGCTTTATCAATTCCTATTGAATATAAGATGTCTGAACTTAGTAACAGACCTTACGCAG
AGTCTAATATTTCATTGAAAAGTGAGCCCATAAATGGGCTCAAAAATACCAATTTAAATATAAAGTGCGTAAGGTCTGTT
AGAAAGAAGATTTTGAGGGATGTTGCTTCCCAGTATTTGCCAGATTATATTGCCTATAGACCAAAGAAAGCCGCACAGTA
TGGAAGTGGTGGGGAGAAGATGATTTATAAGGTTGCTAAGAAATATGGATTTTCAAAGAAGAGAATTAATGAGTTTTTAG
ATATGTTGAAGAGGAAGATTGTTAGTGAATTTTAA

Upstream 100 bases:

>100_bases
TAATCTTTATTAAGATATTTAAATTAAAATTTTGCAAATATTTAGCATAAAATGTTAAGTTGTGTTAGCTAATATTAAAA
AATAAGGTATATTTAAAAAT

Downstream 100 bases:

>100_bases
AATTATAAGCTAGTGTGATACCTATGTTTAAGAAAAAACCATTGATTGGGATGGTTCATCTAAAACCATTGCCTGGTAGT
TATCATTACAATGACAACTT

Product: asparagine synthetase AsnB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MRDMCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMIGNLSLAHNRLAIVGRYGV
QPIPNEDETIWLVCNGEIYNYIELREYLKQNHEFRTDSDNEVIIHLYEEEKLEELDGDYAFAIYDKSKNVVRLARDMFGV
KPLFYVDRDKYFAFASERKALWHLLINIDGCERDLDELNSKIKTLKPNSQLIYYLDDNRFEIIEGFKKLELNYMKERSYE
EAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKLASLYCEVILYAVGTENSEDLIYAERLAKDLNLKLRKKIIS
EEEYEEYVFKVAKAIDEVDLMKIGVGIPIYVASEMANEDGLKVVLSGQGADELFGGYARHERIYRERGEEELKKELLKDV
YNLYKVNLERDDHCTMANGVELRVPFLDEEVVEIALSIPIEYKMSELSNRPYAESNISLKSEPINGLKNTNLNIKCVRSV
RKKILRDVASQYLPDYIAYRPKKAAQYGSGGEKMIYKVAKKYGFSKKRINEFLDMLKRKIVSEF

Sequences:

>Translated_544_residues
MRDMCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMIGNLSLAHNRLAIVGRYGV
QPIPNEDETIWLVCNGEIYNYIELREYLKQNHEFRTDSDNEVIIHLYEEEKLEELDGDYAFAIYDKSKNVVRLARDMFGV
KPLFYVDRDKYFAFASERKALWHLLINIDGCERDLDELNSKIKTLKPNSQLIYYLDDNRFEIIEGFKKLELNYMKERSYE
EAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKLASLYCEVILYAVGTENSEDLIYAERLAKDLNLKLRKKIIS
EEEYEEYVFKVAKAIDEVDLMKIGVGIPIYVASEMANEDGLKVVLSGQGADELFGGYARHERIYRERGEEELKKELLKDV
YNLYKVNLERDDHCTMANGVELRVPFLDEEVVEIALSIPIEYKMSELSNRPYAESNISLKSEPINGLKNTNLNIKCVRSV
RKKILRDVASQYLPDYIAYRPKKAAQYGSGGEKMIYKVAKKYGFSKKRINEFLDMLKRKIVSEF
>Mature_544_residues
MRDMCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMIGNLSLAHNRLAIVGRYGV
QPIPNEDETIWLVCNGEIYNYIELREYLKQNHEFRTDSDNEVIIHLYEEEKLEELDGDYAFAIYDKSKNVVRLARDMFGV
KPLFYVDRDKYFAFASERKALWHLLINIDGCERDLDELNSKIKTLKPNSQLIYYLDDNRFEIIEGFKKLELNYMKERSYE
EAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKLASLYCEVILYAVGTENSEDLIYAERLAKDLNLKLRKKIIS
EEEYEEYVFKVAKAIDEVDLMKIGVGIPIYVASEMANEDGLKVVLSGQGADELFGGYARHERIYRERGEEELKKELLKDV
YNLYKVNLERDDHCTMANGVELRVPFLDEEVVEIALSIPIEYKMSELSNRPYAESNISLKSEPINGLKNTNLNIKCVRSV
RKKILRDVASQYLPDYIAYRPKKAAQYGSGGEKMIYKVAKKYGFSKKRINEFLDMLKRKIVSEF

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=556, Percent_Identity=27.6978417266187, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI168229252, Length=556, Percent_Identity=27.6978417266187, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI168229250, Length=556, Percent_Identity=27.6978417266187, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI296010848, Length=496, Percent_Identity=28.0241935483871, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI296010852, Length=452, Percent_Identity=27.4336283185841, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI296010850, Length=452, Percent_Identity=27.4336283185841, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI9506697, Length=172, Percent_Identity=33.1395348837209, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786889, Length=529, Percent_Identity=29.1115311909263, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71993933, Length=522, Percent_Identity=29.3103448275862, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25147557, Length=522, Percent_Identity=29.3103448275862, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17560178, Length=523, Percent_Identity=28.4894837476099, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25147560, Length=320, Percent_Identity=28.4375, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=464, Percent_Identity=29.0948275862069, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6325403, Length=469, Percent_Identity=28.3582089552239, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6323542, Length=501, Percent_Identity=26.7465069860279, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45553209, Length=538, Percent_Identity=29.368029739777, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 63058; Mature: 63058

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDMCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEE
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCEEEECCCHHHHHHHHH
MIGNLSLAHNRLAIVGRYGVQPIPNEDETIWLVCNGEIYNYIELREYLKQNHEFRTDSDN
HHCCHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
EVIIHLYEEEKLEELDGDYAFAIYDKSKNVVRLARDMFGVKPLFYVDRDKYFAFASERKA
CEEEEEECHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCEEEEHHHHHE
LWHLLINIDGCERDLDELNSKIKTLKPNSQLIYYLDDNRFEIIEGFKKLELNYMKERSYE
EEEEEEECCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHH
EAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKLASLYCEVILYAVGTENSEDL
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
IYAERLAKDLNLKLRKKIISEEEYEEYVFKVAKAIDEVDLMKIGVGIPIYVASEMANEDG
HHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCC
LKVVLSGQGADELFGGYARHERIYRERGEEELKKELLKDVYNLYKVNLERDDHCTMANGV
CEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCEECCCC
ELRVPFLDEEVVEIALSIPIEYKMSELSNRPYAESNISLKSEPINGLKNTNLNIKCVRSV
EEEECCCCHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RKKILRDVASQYLPDYIAYRPKKAAQYGSGGEKMIYKVAKKYGFSKKRINEFLDMLKRKI
HHHHHHHHHHHHCCHHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSEF
HHCC
>Mature Secondary Structure
MRDMCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEE
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCEEEECCCHHHHHHHHH
MIGNLSLAHNRLAIVGRYGVQPIPNEDETIWLVCNGEIYNYIELREYLKQNHEFRTDSDN
HHCCHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
EVIIHLYEEEKLEELDGDYAFAIYDKSKNVVRLARDMFGVKPLFYVDRDKYFAFASERKA
CEEEEEECHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCEEEEHHHHHE
LWHLLINIDGCERDLDELNSKIKTLKPNSQLIYYLDDNRFEIIEGFKKLELNYMKERSYE
EEEEEEECCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHH
EAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKLASLYCEVILYAVGTENSEDL
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
IYAERLAKDLNLKLRKKIISEEEYEEYVFKVAKAIDEVDLMKIGVGIPIYVASEMANEDG
HHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCC
LKVVLSGQGADELFGGYARHERIYRERGEEELKKELLKDVYNLYKVNLERDDHCTMANGV
CEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCEECCCC
ELRVPFLDEEVVEIALSIPIEYKMSELSNRPYAESNISLKSEPINGLKNTNLNIKCVRSV
EEEECCCCHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RKKILRDVASQYLPDYIAYRPKKAAQYGSGGEKMIYKVAKKYGFSKKRINEFLDMLKRKI
HHHHHHHHHHHHCCHHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSEF
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]