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The map label for this gene is trpA [H]

Identifier: 15669227

GI number: 15669227

Start: 969732

End: 970586

Strand: Direct

Name: trpA [H]

Synonym: MJ1038

Alternate gene names: 15669227

Gene position: 969732-970586 (Clockwise)

Preceding gene: 15669226

Following gene: 15669228

Centisome position: 58.24

GC content: 34.39

Gene sequence:

>855_bases
TTGGAGTGGATGATTATGAAATTAGCAGAAAAATTTGAAGAGTTAAAAAACAAAGGAGAAAAGGCATTTGTTGCATTTTA
TGTGGGAGGAGACCCAAACTTGGAAATTTCAGAAAAGGCATTAGAAGTTATTTGTAAGCATGCTGATATTGTTGAGATAG
GTATTCCATTTTCTGACCCTGTTGCAGATGGAATTACAATACAAAAAGCAGATGTTAGAGCTTTAAACAGTGGCATGAAT
CCATTGAAGGCATTTGAGTTGGCTAAAAAATTGAATGAAAAAGCTCCAAATGTTCCAAAGGTGTTCTTAACCTACTATAA
CATAATATTTAAGATGGGAGAGGAGGAGTTCGTTAAAAAATGTAAAGAGGCTGGAGTTTCTGGGATTATAGTTCCAGATT
TGCCAATTGAAGAGGCTGATAGCTTATACAATTACTGTAAAAAGTATGGAGTTGATTTAATATTTTTAGTTGCCCCAACA
ACACCTGATGAAAGGTTAAAGAAAATTTTAGAAAAGTGTAGTGGGTTTGTTTATGTTGTTTCTGTAACTGGGATTACTGG
AGCGAGAGAGAAAGTAGCTGAAGAGACGAAAGAGTTAATTAAGAGAGTTAAAAAGTTTTCAAAAATCCCTGCCTGTGTAG
GGTTTGGAATCTCAAAGAGGGAGCATGTTGAGGAAATAACTGAAATAGCTGATGGAGCTATTGTAGGAAGTGCGATTGTT
AAGATTGTTGAAAAACATTTAGATGAGAATGGACAAATAAAGGATGAGGAAAAATTCCTAAAGGAGTTGGAGGAGTTTGT
TAAAAATCTAAAGGAAGGGACTAAAAAGAAGGCTAAAGTAGCTATAAAAAATTAA

Upstream 100 bases:

>100_bases
CAAGACACCATAGGACTTCGCCCTATTGGGATACCCATTTAAATTAAGATTTTGATCAACCTTTTCTAAAAAGGTTGTTC
GGGCAGCTTTTACTAAAAAG

Downstream 100 bases:

>100_bases
CAGAATATTCAAATAAAGAAATATTTTTATAGGACAATAAAAGTAAAAAATAAATATGAGAAAAAAATATTTGTAAGGAT
GCATCACATTTATTACATCA

Product: tryptophan synthase alpha subunit TrpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MEWMIMKLAEKFEELKNKGEKAFVAFYVGGDPNLEISEKALEVICKHADIVEIGIPFSDPVADGITIQKADVRALNSGMN
PLKAFELAKKLNEKAPNVPKVFLTYYNIIFKMGEEEFVKKCKEAGVSGIIVPDLPIEEADSLYNYCKKYGVDLIFLVAPT
TPDERLKKILEKCSGFVYVVSVTGITGAREKVAEETKELIKRVKKFSKIPACVGFGISKREHVEEITEIADGAIVGSAIV
KIVEKHLDENGQIKDEEKFLKELEEFVKNLKEGTKKKAKVAIKN

Sequences:

>Translated_284_residues
MEWMIMKLAEKFEELKNKGEKAFVAFYVGGDPNLEISEKALEVICKHADIVEIGIPFSDPVADGITIQKADVRALNSGMN
PLKAFELAKKLNEKAPNVPKVFLTYYNIIFKMGEEEFVKKCKEAGVSGIIVPDLPIEEADSLYNYCKKYGVDLIFLVAPT
TPDERLKKILEKCSGFVYVVSVTGITGAREKVAEETKELIKRVKKFSKIPACVGFGISKREHVEEITEIADGAIVGSAIV
KIVEKHLDENGQIKDEEKFLKELEEFVKNLKEGTKKKAKVAIKN
>Mature_284_residues
MEWMIMKLAEKFEELKNKGEKAFVAFYVGGDPNLEISEKALEVICKHADIVEIGIPFSDPVADGITIQKADVRALNSGMN
PLKAFELAKKLNEKAPNVPKVFLTYYNIIFKMGEEEFVKKCKEAGVSGIIVPDLPIEEADSLYNYCKKYGVDLIFLVAPT
TPDERLKKILEKCSGFVYVVSVTGITGAREKVAEETKELIKRVKKFSKIPACVGFGISKREHVEEITEIADGAIVGSAIV
KIVEKHLDENGQIKDEEKFLKELEEFVKNLKEGTKKKAKVAIKN

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=36.3295880149813, Blast_Score=185, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321412, Length=276, Percent_Identity=37.6811594202899, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 31717; Mature: 31717

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWMIMKLAEKFEELKNKGEKAFVAFYVGGDPNLEISEKALEVICKHADIVEIGIPFSDP
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCC
VADGITIQKADVRALNSGMNPLKAFELAKKLNEKAPNVPKVFLTYYNIIFKMGEEEFVKK
CCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHH
CKEAGVSGIIVPDLPIEEADSLYNYCKKYGVDLIFLVAPTTPDERLKKILEKCSGFVYVV
HHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEE
SVTGITGAREKVAEETKELIKRVKKFSKIPACVGFGISKREHVEEITEIADGAIVGSAIV
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
KIVEKHLDENGQIKDEEKFLKELEEFVKNLKEGTKKKAKVAIKN
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHEECCC
>Mature Secondary Structure
MEWMIMKLAEKFEELKNKGEKAFVAFYVGGDPNLEISEKALEVICKHADIVEIGIPFSDP
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCC
VADGITIQKADVRALNSGMNPLKAFELAKKLNEKAPNVPKVFLTYYNIIFKMGEEEFVKK
CCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHH
CKEAGVSGIIVPDLPIEEADSLYNYCKKYGVDLIFLVAPTTPDERLKKILEKCSGFVYVV
HHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEE
SVTGITGAREKVAEETKELIKRVKKFSKIPACVGFGISKREHVEEITEIADGAIVGSAIV
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
KIVEKHLDENGQIKDEEKFLKELEEFVKNLKEGTKKKAKVAIKN
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]