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The map label for this gene is trpB

Identifier: 15669226

GI number: 15669226

Start: 968341

End: 969555

Strand: Direct

Name: trpB

Synonym: MJ1037

Alternate gene names: 15669226

Gene position: 968341-969555 (Clockwise)

Preceding gene: 15669225

Following gene: 15669227

Centisome position: 58.16

GC content: 38.11

Gene sequence:

>1215_bases
GTGTCCATCTTGAAGAAATACAAAGACATGTATCCAGATGAAAATGGAAAGTTTGGCATTTACGGAGGTAAATTTGTTCC
AGAAACATTGATGCCAGCCATAGCTGAATTGGAAGAAGCATTTAAGAGATTTTGGATAAACAATGAAGGAAACTTTAGAG
AAGAGTTCTATGCTTTATTGAGGGATTACGTTGGAAGACCAACACCATTATATTATGCTGAGAGATTGAGTGAAGAACTT
GGTTGTAAAGTTTATTTAAAGAGAGAAGATTTAGCACACTTAGGAGCTCATAAAATAAACAACGCCTTAGGACAAGCTTT
GTTAGCTAAAAAGATGGGTAAAAAGAGAGTTATAGCTGAAACTGGAGCTGGACAGCATGGAGTTGCTACTGCAGCAGCAT
GTGCAAAGCTTGGATTAGAATGCATAATATACATGGGAGCTAAAGACGTTGAAAGGCAAAAATTAAATGTTTTTAGGATG
GAGTTGATGGGAGCTAAGGTTATTCCAGTATTTGGAGGCTCACAGACATTAAAAGATGCTGTAAATGAAGCTTTGAGAGA
TTGGACAACCAACGTTAGAACTACCTACTATTTGCTTGGTTCTGCCTTAGGACCTCATCCATACCCAATGATGGTTAGAG
AATTCCAAAGAGTTATTGGTAAAGAACTTAAAGAGCAGATATTAGAAAAAGAGGGAAGATTGCCAGATGTTATCGTTGCA
TGTGTTGGAGGAGGAAGTAATGCAATAGGGGCATTTTATGAGTTTTTGGATGATGACGTAGAGCTTTACGCTGTTGAGGC
TGGAGGTAAAGGGATAGAAACTGGAATGCATGGAGCTTCATTATGTGCTGGAGAGGTTGGAGTTTTGCATGGAGCTAAGA
TTTATGTTAAAGAGGATGAGTTTGGGCAGATAGAAGAAAGTTACAGTATTTCAGCAGGTTTAGATTATCCTGGAGTAGGA
CCTGAGCTTTCATTTTTAAAGGATGAGGGAAGGATTAAAGCAGTTTGTGTAACTGATGACGAAGCTTTAGAGGCATTTCA
ATTGCTATGTAGGTTAGAAGGTATTTTACCAGCATTGGAAAGTTCTCATGCCTTAGCTTATGCTGTTAAATTGGCTGATA
AATTAGATAAAGATGATATAATGGTTATTAATTTGTCTGGAAGAGGGGATAAGGACGTTCAGACTGTTGCTAAGGCATTG
GGAAGGGAGATTTAA

Upstream 100 bases:

>100_bases
TGGGCTATATATTGGTTGGAATCATTGCATTGCATTAAGTTCAAAACGTATAAGTATTACTACCAAATACTATTTCAGAA
GCTAAAATTATCTTTTTGGG

Downstream 100 bases:

>100_bases
AGACATATTCGATTACTTGAGACAATCTTAAGAGACATTAAAGGGACTAAAAGCCCTTTAATGTATCCGTTCTGGTCAAG
ACACCATAGGACTTCGCCCT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSILKKYKDMYPDENGKFGIYGGKFVPETLMPAIAELEEAFKRFWINNEGNFREEFYALLRDYVGRPTPLYYAERLSEEL
GCKVYLKREDLAHLGAHKINNALGQALLAKKMGKKRVIAETGAGQHGVATAAACAKLGLECIIYMGAKDVERQKLNVFRM
ELMGAKVIPVFGGSQTLKDAVNEALRDWTTNVRTTYYLLGSALGPHPYPMMVREFQRVIGKELKEQILEKEGRLPDVIVA
CVGGGSNAIGAFYEFLDDDVELYAVEAGGKGIETGMHGASLCAGEVGVLHGAKIYVKEDEFGQIEESYSISAGLDYPGVG
PELSFLKDEGRIKAVCVTDDEALEAFQLLCRLEGILPALESSHALAYAVKLADKLDKDDIMVINLSGRGDKDVQTVAKAL
GREI

Sequences:

>Translated_404_residues
MSILKKYKDMYPDENGKFGIYGGKFVPETLMPAIAELEEAFKRFWINNEGNFREEFYALLRDYVGRPTPLYYAERLSEEL
GCKVYLKREDLAHLGAHKINNALGQALLAKKMGKKRVIAETGAGQHGVATAAACAKLGLECIIYMGAKDVERQKLNVFRM
ELMGAKVIPVFGGSQTLKDAVNEALRDWTTNVRTTYYLLGSALGPHPYPMMVREFQRVIGKELKEQILEKEGRLPDVIVA
CVGGGSNAIGAFYEFLDDDVELYAVEAGGKGIETGMHGASLCAGEVGVLHGAKIYVKEDEFGQIEESYSISAGLDYPGVG
PELSFLKDEGRIKAVCVTDDEALEAFQLLCRLEGILPALESSHALAYAVKLADKLDKDDIMVINLSGRGDKDVQTVAKAL
GREI
>Mature_403_residues
SILKKYKDMYPDENGKFGIYGGKFVPETLMPAIAELEEAFKRFWINNEGNFREEFYALLRDYVGRPTPLYYAERLSEELG
CKVYLKREDLAHLGAHKINNALGQALLAKKMGKKRVIAETGAGQHGVATAAACAKLGLECIIYMGAKDVERQKLNVFRME
LMGAKVIPVFGGSQTLKDAVNEALRDWTTNVRTTYYLLGSALGPHPYPMMVREFQRVIGKELKEQILEKEGRLPDVIVAC
VGGGSNAIGAFYEFLDDDVELYAVEAGGKGIETGMHGASLCAGEVGVLHGAKIYVKEDEFGQIEESYSISAGLDYPGVGP
ELSFLKDEGRIKAVCVTDDEALEAFQLLCRLEGILPALESSHALAYAVKLADKLDKDDIMVINLSGRGDKDVQTVAKALG
REI

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=388, Percent_Identity=60.8247422680412, Blast_Score=467, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321412, Length=397, Percent_Identity=57.1788413098237, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_METJA (Q60179)

Other databases:

- EMBL:   L77117
- PIR:   D64429
- RefSeq:   NP_248031.1
- ProteinModelPortal:   Q60179
- SMR:   Q60179
- GeneID:   1451934
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1037
- NMPDR:   fig|243232.1.peg.1068
- TIGR:   MJ1037
- HOGENOM:   HBG303148
- OMA:   TFENTHY
- ProtClustDB:   PRK04346
- BioCyc:   MJAN243232:MJ_1037-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 44365; Mature: 44233

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILKKYKDMYPDENGKFGIYGGKFVPETLMPAIAELEEAFKRFWINNEGNFREEFYALL
CCHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RDYVGRPTPLYYAERLSEELGCKVYLKREDLAHLGAHKINNALGQALLAKKMGKKRVIAE
HHHCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TGAGQHGVATAAACAKLGLECIIYMGAKDVERQKLNVFRMELMGAKVIPVFGGSQTLKDA
CCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHH
VNEALRDWTTNVRTTYYLLGSALGPHPYPMMVREFQRVIGKELKEQILEKEGRLPDVIVA
HHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
CVGGGSNAIGAFYEFLDDDVELYAVEAGGKGIETGMHGASLCAGEVGVLHGAKIYVKEDE
EECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHCCHHEECCCEEEEECCC
FGQIEESYSISAGLDYPGVGPELSFLKDEGRIKAVCVTDDEALEAFQLLCRLEGILPALE
CCCHHHCCCCCCCCCCCCCCCCHHHEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHC
SSHALAYAVKLADKLDKDDIMVINLSGRGDKDVQTVAKALGREI
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SILKKYKDMYPDENGKFGIYGGKFVPETLMPAIAELEEAFKRFWINNEGNFREEFYALL
CHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RDYVGRPTPLYYAERLSEELGCKVYLKREDLAHLGAHKINNALGQALLAKKMGKKRVIAE
HHHCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TGAGQHGVATAAACAKLGLECIIYMGAKDVERQKLNVFRMELMGAKVIPVFGGSQTLKDA
CCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHH
VNEALRDWTTNVRTTYYLLGSALGPHPYPMMVREFQRVIGKELKEQILEKEGRLPDVIVA
HHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
CVGGGSNAIGAFYEFLDDDVELYAVEAGGKGIETGMHGASLCAGEVGVLHGAKIYVKEDE
EECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHCCHHEECCCEEEEECCC
FGQIEESYSISAGLDYPGVGPELSFLKDEGRIKAVCVTDDEALEAFQLLCRLEGILPALE
CCCHHHCCCCCCCCCCCCCCCCHHHEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHC
SSHALAYAVKLADKLDKDDIMVINLSGRGDKDVQTVAKALGREI
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087